| Budget Amount *help |
¥45,110,000 (Direct Cost: ¥34,700,000、Indirect Cost: ¥10,410,000)
Fiscal Year 2023: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2022: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2021: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2020: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2019: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
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| Outline of Final Research Achievements |
This study aimed to elucidate the functions of the pollen S determinant SFB, which plays a crucial role in the self-incompatibility response, and the general inhibitor inactivating pistil S determinant S-RNase in Prunus fruit trees. Additionally, it sought to clarify the function of pollen part modifier MGST in Prunus. Through this research, we were able to experimentally demonstrate that SFB functions as a pollen S determinant. On the other hand, we found no evidence from any of the experiments for the polyubiquitination of S-RNase by SLFLs or SFBL, which were hypothesized to be general inhibitor inactivating S-RNase, indicating the need to reconsider our working hypothesis. The experimental results using antisense oligonucleotides not only demonstrated the functions of MGST and SFB but also suggested potential methods for artificially breaking self-incompatibility.
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