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Molecular characterization of virulence mechanisms of shrimp pathogenic virus WSSV

Research Project

Project/Area Number 19H00949
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

Hirono Ikuo  東京海洋大学, 学術研究院, 教授 (00270926)

Co-Investigator(Kenkyū-buntansha) 近藤 秀裕  東京海洋大学, 学術研究院, 教授 (20314635)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥45,630,000 (Direct Cost: ¥35,100,000、Indirect Cost: ¥10,530,000)
Fiscal Year 2021: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2020: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2019: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Keywordsクルマエビ / 病原ウイルス / ホワイトスポット病 / 化石ウイルスゲノム / ホワイトスポット病ウイルス / WSSV / 化石ウイルス
Outline of Research at the Start

本研究ではクルマエビの病原ウイルスであるWSSVの類似化石ウイルスを復元し、その復元化石ウイルスを用いてWSSVの病原性メカニズムの解明を目的とし、①クルマエビ類ゲノムに存在するWSSV類似化石ウイルスの復元、②WSSV特異的遺伝子を化石ウイルスに導入したキメラウイルスによる病原性メカニズムの解明および③WSSV類似化石ウイルスゲノムに存在する遺伝子から転写されるmRNAの機能について解明を行う。さらに、これらの研究を通してクルマエビ類のWSSV感染症防除法開発の基盤技術の構築に努める。

Outline of Final Research Achievements

White spot syndrome virus (WSSV) and WSSV like fossilized virus genomes were amplified by the long PCR method to obtain a region covering the entire genome as seven DNA fragments. We attempted to introduce foreign DNA into the cells of the shrimp using various reagents and electroporation methods, but no expression of the introduced genes was observed. We also tried to introduce DNA into primary cultured cells of the shrimp, but were unable to do so. It is necessary to examine whether there is a problem in the expression of the introduced gene. Comparison of genomes of WSSV of different isolates showed differences in sequences where amino acid substitutions occur, suggesting that several genes are associated with virulence intensity. We obtained sequences transcribed from genes encoded in WSSV like fossilized virus genomes.

Translated with www.DeepL.com/Translator (free version)

Academic Significance and Societal Importance of the Research Achievements

WSSV感染症はクルマエビ類養殖における最大の脅威の一つである。WSSV感染症の克服はクルマエビ類養殖の持続的な発展に重要な課題である。本研究ではクルマエビ類を始めとする甲殻類のゲノム中にWSSV類似ウイルスのゲノムが化石化してマルチコピーで存在することを明らかにした。WSSVに類似の現存するウイルスの存在は知られていなかったが、ズワイガニから分離されたウイルスのゲノムを解読し、WSSV近縁のウイルスであることを明らかにした。分離年や地域の異なるWSSVは病原性が異なることがわかり、それらのゲノムを比較解析したところ、WSSVの病原性に関連する可能性がある遺伝子を見つけることができた。

Report

(5 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Comments on the Screening Results   Annual Research Report
  • Research Products

    (19 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (3 results) Journal Article (7 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 7 results,  Open Access: 3 results) Presentation (9 results) (of which Int'l Joint Research: 5 results,  Invited: 4 results)

  • [Int'l Joint Research] Kasetsart University/Walailak University/タイ水産局(タイ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Kasetsart University/Walailak University/タイ水産局(タイ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Kasetsart University/Department of Fisheries(タイ)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Transcriptome profiling reveals the novel immunometabolism-related genes against WSSV infection from Fenneropenaeus merguiensis2022

    • Author(s)
      Jaree Phattarunda、Boonchuen Pakpoom、Thawonsuwan Jumroensri、Kondo Hidehiro、Hirono Ikuo、Somboonwiwat Kunlaya
    • Journal Title

      Fish & Shellfish Immunology

      Volume: 120 Pages: 31-44

    • DOI

      10.1016/j.fsi.2021.11.006

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Bacterial and eukaryotic communities in pond water of whiteleg shrimp Litopenaeus vannamei and the bacterial communities of their stomach and midgut2022

    • Author(s)
      Imaizumi Kentaro、Molex Wanlapha、Jitnavee Chakrit、Direkbusarakom Sataporn、Kondo Hidehiro、Hirono Ikuo
    • Journal Title

      Aquaculture

      Volume: 554 Pages: 738139-738139

    • DOI

      10.1016/j.aquaculture.2022.738139

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Genome and transcriptome assemblies of the kuruma shrimp, <i>Marsupenaeus japonicus</i>2021

    • Author(s)
      Kawato Satoshi、Nishitsuji Koki、Arimoto Asuka、Hisata Kanako、Kawamitsu Mayumi、Nozaki Reiko、Kondo Hidehiro、Shinzato Chuya、Ohira Tsuyoshi、Satoh Noriyuki、Shoguchi Eiichi、Hirono Ikuo
    • Journal Title

      G3 Genes|Genomes|Genetics

      Volume: 11 Issue: 11

    • DOI

      10.1093/g3journal/jkab268

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding.2021

    • Author(s)
      Imaizumi K, Tinwongger S, Kondo H, Hirono I.
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 9936-9936

    • DOI

      10.1038/s41598-021-89415-w

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An oral delivery system for controlling white pot syndrome virus infection in shrimp using transgenic microalgae.2020

    • Author(s)
      Kiataramgul A, Maneenin S, Purton S, Areechon N, Hirono I, Brocklehurst TW, Unajak S.
    • Journal Title

      Aquaculture

      Volume: 521 Pages: 735022-735022

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] An oral delivery system for controlling white spot syndrome virus infection in shrimp using transgenic microalgae2020

    • Author(s)
      Kiataramgul Asama、Maneenin Sugunya、Purton Saul、Areechon Nontawith、Hirono Ikuo、Brocklehurst Thanyanan Wannathong、Unajak Sasimanas
    • Journal Title

      Aquaculture

      Volume: 521 Pages: 735022-735022

    • DOI

      10.1016/j.aquaculture.2020.735022

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A Hint of Primitive Mucosal Immunity in Shrimp through Marsupenaeus japonicus Gill C-Type Lectin2019

    • Author(s)
      Alenton Rod Russel R.、Koiwai Keiichiro、Nakamura Rika、Thawonsuwan Jumroensri、Kondo Hidehiro、Hirono Ikuo
    • Journal Title

      The Journal of Immunology

      Volume: 203 Issue: 8 Pages: 2310-2318

    • DOI

      10.4049/jimmunol.1900156

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Development of preventive methods for shrimp infectious diseases using safe and effective microorganisms.2021

    • Author(s)
      Ikuo Hirono
    • Organizer
      3rd International Control of Aquatic Animal Diseases (CAAD) Symposium 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] クルマエビのドラフトゲノム2021

    • Author(s)
      川戸智・西辻光希・有本飛鳥・久田香奈子・川満真由美・野崎玲子・近藤秀裕・新里宙也・大平剛・佐藤矩行・將口栄一・廣野育生
    • Organizer
      第21回マリンバイオテクノロジー学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Characterization of mitotic checkpoint genes and its expression profile during cellular arrest in Kuruma shrimp (Marsupenaeus japonicus) cell culture2021

    • Author(s)
      Thitiporn Thammasorn, Reiko Nozaki, Hidehiro Kondo, and Ikuo Hirono,
    • Organizer
      3rd International Control of Aquatic Animal Diseases (CAAD) Symposium 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] タイ王国のバナメイエビ養殖場における池水中およびエビ消化管中の微生物叢の動態2021

    • Author(s)
      今泉健太郎・近藤秀裕(海洋大)・Sataporn Direkbusarakom(ワライラック大)・廣野育生
    • Organizer
      令和3年度日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] High-throughput genetic screens for the development of continuous shrimp cell line from testicular tissue of Marsupenaeus japonicus2020

    • Author(s)
      Thitiporn Thammasorn、Reiko Nozaki、Hidehiro Kondo、Ikuo Hirono
    • Organizer
      令和2年度日本水産学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Studies on development of prevention methods against shrimp infections diseases2020

    • Author(s)
      Ikuo Hirono
    • Organizer
      International Symposium: The Control of Aquatic Animal Diseases
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 宿主ゲノムに残された足跡が語るエビ病原ウイルスの起源と進化2019

    • Author(s)
      川戸 智、近藤 秀裕、廣野 育生
    • Organizer
      第67回日本ウイルス学会学術集会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Genomic footprints unveil the evolution of obscure crustacean virus family2019

    • Author(s)
      Satoshi Kawato,Reiko Nozaki,Hidehiro Kondo,Ikuo Hirono
    • Organizer
      国際マリンバイオテクノロジー学会2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular characterization of shrimp hemocytes2019

    • Author(s)
      Koiwai K, Kondo H, Hirono, I.
    • Organizer
      The 14 th International Symposium of the Protein Society of Thailand
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2019-04-18   Modified: 2024-01-30  

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