| Budget Amount *help |
¥44,850,000 (Direct Cost: ¥34,500,000、Indirect Cost: ¥10,350,000)
Fiscal Year 2023: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2022: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2021: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2020: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2019: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Outline of Final Research Achievements |
Eukaryotic chemotaxis plays an important role as a cellular function to control the direction of cell motility in environmental exploration of unicellular organisms and in development and immune responses of multicellular organisms. In this study, we analyzed the intracellular spatiotemporal dynamics of signaling molecules involved in chemotaxis using super-resolution and single-molecule microscopy to elucidate the gradient sensing mechanism. We revealed the mechanism by which the concentration gradient is recognized over a wide concentration range, which includes not only phosphorylation of GPCR-type chemoattractant receptors, but also regulation of the activity as well as the membrane-cytosol shuttling of G proteins and lateral diffusion on the membrane of G proteins. In addition, novel findings were obtained on the excitable system that acts to amplify the gradient signals, and we proposed a new mechanism of 1/0 signal formation, which we termed "dynamic partitioning".
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