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Elucidation of gradient sensing mechanism in chemotactic signaling system

Research Project

Project/Area Number 19H00982
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
Research InstitutionOsaka University

Principal Investigator

Ueda Masahiro  大阪大学, 大学院生命機能研究科, 教授 (40444517)

Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥44,850,000 (Direct Cost: ¥34,500,000、Indirect Cost: ¥10,350,000)
Fiscal Year 2023: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2022: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2021: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2020: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2019: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Keywords走化性 / GPCR / 三量体Gタンパク質 / アレスチン / 適応 / 細胞性粘菌 / 極性形成 / 1分子イメージング / 三量体G蛋白質
Outline of Research at the Start

細胞は誘引物質の濃度勾配を認識し、濃度の高い方に向かって移動できます。単細胞からヒト細胞まで広く見られ、走化性と呼ばれます。最近我々は、三量体G蛋白質が細胞質と細胞膜の間で局在を変えることによって走化性シグナルを伝達するという、これまでに全く知られていない仕組みを発見しました。本研究ではその仕組みを解明し「G蛋白質局在制御によるGPCRシグナリングの調節」という新しいコンセプトの確立を目指します。

Outline of Final Research Achievements

Eukaryotic chemotaxis plays an important role as a cellular function to control the direction of cell motility in environmental exploration of unicellular organisms and in development and immune responses of multicellular organisms. In this study, we analyzed the intracellular spatiotemporal dynamics of signaling molecules involved in chemotaxis using super-resolution and single-molecule microscopy to elucidate the gradient sensing mechanism. We revealed the mechanism by which the concentration gradient is recognized over a wide concentration range, which includes not only phosphorylation of GPCR-type chemoattractant receptors, but also regulation of the activity as well as the membrane-cytosol shuttling of G proteins and lateral diffusion on the membrane of G proteins. In addition, novel findings were obtained on the excitable system that acts to amplify the gradient signals, and we proposed a new mechanism of 1/0 signal formation, which we termed "dynamic partitioning".

Academic Significance and Societal Importance of the Research Achievements

本研究を通して明らかとなった広い濃度域にわたる濃度勾配認識の仕組みやダイナミックパーティショニングの仕組みは、下等真核生物である細胞性粘菌において初めて発見された仕組みであり、現在のところ普遍性は明らかになっていない。しかしながら、走化性シグナル伝達系はヒト免疫細胞を含む様々な真核生物で広く保存されていることから、他の細胞種においても同様の制御機構が働く可能性が示唆される。またGPCRは細胞内シグナル伝達において中心的な役割を果たしていることから、新たな制御メカニズムの基礎的な発見と解明は、医科学・創薬などにおける疾患の原因究明や治療薬の開発を進める上で不可欠な意義を有する。

Report

(7 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Comments on the Screening Results   Annual Research Report
  • Research Products

    (98 results)

All 2024 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (5 results) Journal Article (26 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 17 results,  Open Access: 19 results) Presentation (56 results) (of which Int'l Joint Research: 10 results,  Invited: 18 results) Remarks (8 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 2 results)

  • [Int'l Joint Research] ジョンズホプキンス大学/NIH(米国)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] ジョンズホプキンス大学/NIH(米国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] ジョンズホプキンス大学/NIH(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Johns Hopkins University/NIH(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Johns Hopkins University/NIH(米国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Cholesterol suppresses spontaneous activation of EGFR-mediated signal transduction2024

    • Author(s)
      Takayama Miri、Maeda Sakura、Watanabe Daisuke、Takebayashi Kazutoshi、Hiroshima Michio、Ueda Masahiro
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 704 Pages: 149673-149673

    • DOI

      10.1016/j.bbrc.2024.149673

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Left-right Myosin-Is, Myosin1C, and Myosin1D exhibit distinct single molecule behaviors on the plasma membrane of Drosophila macrophages2024

    • Author(s)
      Utsunomiya Sosuke、Takebayashi Kazutoshi、Yamaguchi Asuka、Sasamura Takeshi、Inaki Mikiko、Ueda Masahiro、Matsuno Kenji
    • Journal Title

      Genes to Cells

      Volume: 29 Issue: 5 Pages: 380-396

    • DOI

      10.1111/gtc.13110

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spontaneous signal generation by an excitable system for cell migration2024

    • Author(s)
      Matsuoka Satomi、Iwamoto Koji、Shin Da Young、Ueda Masahiro
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 12 Pages: 1373609-1373609

    • DOI

      10.3389/fcell.2024.1373609

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Automated single-molecule imaging for drug discovery2024

    • Author(s)
      Hiroshima M., Ueda M
    • Journal Title

      Proc. of SPIE.

      Volume: 12853 Pages: 1285308-1285308

    • DOI

      10.1117/12.3009718

    • Related Report
      2023 Annual Research Report
  • [Journal Article] Sphingomyelin metabolism underlies Ras excitability for efficient cell migration and chemotaxis2023

    • Author(s)
      Shin Da Young、Takagi Hiroaki、Hiroshima Michio、Matsuoka Satomi、Ueda Masahiro
    • Journal Title

      Cell Structure and Function

      Volume: 48 Issue: 2 Pages: 145-160

    • DOI

      10.1247/csf.23045

    • ISSN
      0386-7196, 1347-3700
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A dynamic partitioning mechanism polarizes membrane protein distribution2023

    • Author(s)
      Banerjee Tatsat、Matsuoka Satomi、Biswas Debojyoti、Miao Yuchuan、Pal Dhiman Sankar、Kamimura Yoichiro、Ueda Masahiro、Devreotes Peter N.、Iglesias Pablo A.
    • Journal Title

      Nature Communications

      Volume: 14 Issue: 1 Pages: 7909-7909

    • DOI

      10.1038/s41467-023-43615-2

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Single-molecule tracking-based drug screening2023

    • Author(s)
      Watanabe Daisuke、Hiroshima Michio、Ueda Masahiro
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/2023.11.12.566743

    • Related Report
      2023 Annual Research Report
    • Open Access
  • [Journal Article] RasGEFX triggers spontaneous Ras excitation with RasGEFB/M/U for random cell migration2023

    • Author(s)
      Iwamoto Koji、Matsuoka Satomi、Ueda Masahiro
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/2023.08.13.553116

    • Related Report
      2023 Annual Research Report
    • Open Access
  • [Journal Article] Field model for multistate lateral diffusion of various transmembrane proteins observed in living Dictyostelium cells2023

    • Author(s)
      Takebayashi, K., Kamimura, Y. and Ueda M.
    • Journal Title

      Journal of Cell Science

      Volume: 136 Issue: 4

    • DOI

      10.1242/jcs.260280

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The specific core fucose-binding lectin Pholiota squarrosa lectin (PhoSL) inhibits hepatitis B virus infection in vitro2023

    • Author(s)
      3.Ouchida, T., Maeda, H., Akamatsu, Y., Maeda, M., Takamatsu, S., Kondo, J., Misaki, R., Kamada, Y., Ueda, M., Ueda, K. and Miyoshi, E.
    • Journal Title

      Scientific Report

      Volume: 13 Issue: 1 Pages: 6175-6175

    • DOI

      10.1038/s41598-023-28572-6

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 総論 生命システムにおけるゆらぎの階層性2023

    • Author(s)
      上田 昌宏
    • Journal Title

      細胞

      Volume: 55 Pages: 2-3

    • Related Report
      2022 Annual Research Report
  • [Journal Article] 1分子イメージングの全自動化と大規模解析への応用2023

    • Author(s)
      廣島通夫, 上田昌宏
    • Journal Title

      季刊誌 『生産と技術』

      Volume: 75 Pages: 77-81

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Calcium responses to external mechanical stimuli in the multicellular stage of Dictyostelium discoideum2022

    • Author(s)
      Hashimura Hidenori、Morimoto Yusuke V.、Hirayama Yusei、Ueda Masahiro
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 12428-12428

    • DOI

      10.1038/s41598-022-16774-3

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 走化性のGPCRシグナリング2022

    • Author(s)
      上田 昌宏, 上村 陽一郎
    • Journal Title

      BIO Clinica

      Volume: 37 Pages: 26-30

    • Related Report
      2022 Annual Research Report
  • [Journal Article] SRC kinase activator CDCP1 promotes hepatocyte growth factor-induced cell migration/invasion of a subset of breast cancer cells2022

    • Author(s)
      Kawase Naoyuki、Sugihara Atsuya、Kajiwara Kentaro、Hiroshima Michio、Akamatsu Kanako、Nada Shigeyuki、Matsumoto Kunio、Ueda Masahiro、Okada Masato
    • Journal Title

      Journal of Biological Chemistry

      Volume: 298 Issue: 3 Pages: 101630-101630

    • DOI

      10.1016/j.jbc.2022.101630

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Heterotrimeric Gq proteins?act as a switch for GRK5/6 selectivity underlying β-arrestin transducer bias2022

    • Author(s)
      Kawakami Kouki、Yanagawa Masataka、Hiratsuka Suzune、Yoshida Misaki、Ono Yuki、Hiroshima Michio、Ueda Masahiro、Aoki Junken、Sako Yasushi、Inoue Asuka
    • Journal Title

      Nature Communications

      Volume: 13 Issue: 1 Pages: 870-870

    • DOI

      10.1038/s41467-022-28056-7

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 特集 生物物理学の進歩-生命現象の定量的理解へ向けて Ⅱ.細胞レベル 運動する細胞の前後極性形成のための自発シグナル生成メカニズム2021

    • Author(s)
      松岡 里実、上田 昌宏
    • Journal Title

      生体の科学

      Volume: 72 Issue: 3 Pages: 229-233

    • DOI

      10.11477/mf.2425201353

    • ISSN
      0370-9531, 1883-5503
    • Year and Date
      2021-06-15
    • Related Report
      2021 Annual Research Report
  • [Journal Article] Comparative Analysis of Single-Molecule Dynamics of TRPV1 and TRPV4 Channels in Living Cells2021

    • Author(s)
      Kuwashima Yutaro、Yanagawa Masataka、Abe Mitsuhiro、Hiroshima Michio、Ueda Masahiro、Arita Makoto、Sako Yasushi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 16 Pages: 8473-8473

    • DOI

      10.3390/ijms22168473

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Different Heterotrimeric G Protein Dynamics for Wide-Range Chemotaxis in Eukaryotic Cells2021

    • Author(s)
      Kamimura Yoichiro、Ueda Masahiro
    • Journal Title

      Frontiers in Cell and Developmental Biology

      Volume: 9 Pages: 724797-724797

    • DOI

      10.3389/fcell.2021.724797

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] GPCR signaling regulation in Dictyostelium chemotaxis2021

    • Author(s)
      Kamimura,Y. and Ueda, M
    • Journal Title

      Method in Molecular Biology

      Volume: in press

    • Related Report
      2020 Annual Research Report
  • [Journal Article] A sub-population of Dictyostelium discoideum cells shows extremely high sensitivity to cAMP for directional migration2021

    • Author(s)
      Ohtsuka, D., Ota, N., Amaya, S., Matsuoka, S., Tanaka, Y., and Ueda, M.
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 554 Pages: 131-137

    • DOI

      10.1016/j.bbrc.2021.03.095

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Talin B regulates collective cell migration via PI3K signaling in Dictyostelium discoideum mounds2020

    • Author(s)
      Shin-Ichi Yamazaki, Hidenori Hashimura, Yusuke V. Morimoto, Yukihiro Miyanaga, Satomi Matsuoka, Yoichiro Kamimura, Masahiro Ueda
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 525 Issue: 2 Pages: 372-377

    • DOI

      10.1016/j.bbrc.2020.02.060

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Single-molecule imaging of PI(4,5)P2 and PTEN in vitro reveals a positive feedback mechanism for PTEN membrane binding2020

    • Author(s)
      2.Yoshioka, D., Fukushima, S., Koteishi, H., Okuno, D., Ide, T., Matsuoka, S., and Ueda, M.
    • Journal Title

      Communications Biology

      Volume: 3 Issue: 1 Pages: 92-92

    • DOI

      10.1038/s42003-020-0818-3

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intracellular ATP levels influence cell fates in Dictyostelium discoideum differentiation2020

    • Author(s)
      Hiraoka Haruka、Nakano Tadashi、Kuwana Satoshi、Fukuzawa Masashi、Hirano Yasuhiro、Ueda Masahiro、Haraguchi Tokuko、Hiraoka Yasushi
    • Journal Title

      Genes to Cells

      Volume: - Issue: 5 Pages: 312-326

    • DOI

      10.1111/gtc.12763

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Large scale single-molecule imaging aided by artificial intelligence2020

    • Author(s)
      Hiroshima, M., Yasui, M., and Ueda, M.
    • Journal Title

      Microscopy

      Volume: 69 Issue: 2 Pages: 69-78

    • DOI

      10.1093/jmicro/dfz116

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 三量体G蛋白質シャトリング制御2020

    • Author(s)
      上村 陽一郎、上田 昌宏
    • Journal Title

      医学のあゆみ

      Volume: 272 Pages: 605-606

    • Related Report
      2019 Annual Research Report
  • [Presentation] RasGEFX は Ras の自発的興奮を誘発し、RasGEFB/M/U とともにランダムな細胞運動を駆動する2024

    • Author(s)
      岩本浩司、松岡里実、上田昌宏
    • Organizer
      生体運動研究2024合同班会議
    • Related Report
      2023 Annual Research Report
  • [Presentation] 真核生物の走化性においてβ-arrestinは三量体G蛋白質シグナルに抑制的に働く2024

    • Author(s)
      室本匡希、松岡里実、上田昌宏
    • Organizer
      生体運動研究2024合同班会議
    • Related Report
      2023 Annual Research Report
  • [Presentation] Automated single-molecule imaging for drug discovery2024

    • Author(s)
      Michio Hiroshima, Masahiro Ueda
    • Organizer
      SPIE 2024 Photonics West BiOS
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Field model for multistate lateral diffusion of various transmembrane proteins observed in living Dictyostelium cells2023

    • Author(s)
      Kazutoshi Takebayashi, Yoichiro Kamimura, Masahiro Ueda
    • Organizer
      Imaging Cell Dynamics Meeting
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Signal generation by an excitable system for cell migration”, STATPHYS28 Satellite Meeting: Statistical Physics and Information-Processing in Living Systems,2023

    • Author(s)
      Satomi Matsuoka, Masahiro Ueda
    • Organizer
      STATPHYS28 Satellite Meeting: Statistical Physics and Information-Processing in Living Systems
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] RasGEFX triggers spontaneous Ras excitation with RasGEFB/M/U for random cell migration2023

    • Author(s)
      Koji Iwamoto, Satomi Matsuoka, Masahiro Ueda
    • Organizer
      Dicty 2023, Annual International Dictyostelium Conference
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Beta-arrestin orchestrates the extension of the concentration ranges in eukaryotic chemotaxis2023

    • Author(s)
      Masaki Muromoto, Satomi Matsuoka, Masahiro Ueda
    • Organizer
      Dicty 2023, Annual International Dictyostelium Conference
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ield model for multistate lateral diffusion of various transmembrane proteins observed in living cells2023

    • Author(s)
      Kazutoshi Takebayashi, Yoichiro Kamimura, Masahiro Ueda
    • Organizer
      Dicty 2023, Annual International Dictyostelium Conference
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sphingomyelin metabolism underlies Ras excitability for efficient cell migration and chemotaxis2023

    • Author(s)
      Satomi Matsuoka, Da Young Shin, Masahiro Ueda
    • Organizer
      Dicty 2023, Annual International Dictyostelium Conference
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 1分子動態を指標とした薬剤スクリーニング法の開発2023

    • Author(s)
      渡邉大介、廣島通夫、上田昌宏
    • Organizer
      研究会「理論と実験」2023
    • Related Report
      2023 Annual Research Report
  • [Presentation] 真核生物の走化性におけるβ-arrestinの役割2023

    • Author(s)
      室本匡希、松岡里実、上田昌宏
    • Organizer
      第13回日本細胞性粘菌学会例会
    • Related Report
      2023 Annual Research Report
  • [Presentation] RasGEFX triggers spontaneous Ras excitation with RasGEFB/M/U for random cell migration2023

    • Author(s)
      岩本浩司、松岡里実、上田昌宏
    • Organizer
      第13回日本細胞性粘菌学会例会
    • Related Report
      2023 Annual Research Report
  • [Presentation] The Dictyostelium chemotaxis receptor cAR1 more tightly gathers on the front side of the cell2023

    • Author(s)
      三井敦弘、松岡里実、上田昌宏
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Identification of GAP that suppresses the excitatory Ras in spontaneous cell motility2023

    • Author(s)
      Guangyu Cheng, Satomi Matsuoka, Masahiro Ueda
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] β-arrestin acts as an inhibitor of trimeric G protein signaling in eukaryotic chemotaxis2023

    • Author(s)
      室本匡希、松岡里実、上田昌宏
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] RasGEFX triggers spontaneous Ras excitation with RasGEFB/M/U for random cell migration2023

    • Author(s)
      岩本浩司、松岡里実、上田昌宏
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Single-molecule analysis of EGFR behavior inducing signal transduction in the plasma membrane2023

    • Author(s)
      Michio Hiroshima, Masahiro Ueda
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Phosphatidylserine is an essential regulator of Ras excitability and cell motility2023

    • Author(s)
      Satomi Matsuoka, Da Young Shin, Hyeyun Jung, Hiroaki Takagi, Michio Hiroshima, Masahiro Ueda
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 細胞運動における興奮系による前後極性の自己組織化2023

    • Author(s)
      松岡里実、上田昌宏
    • Organizer
      第46回日本分子生物学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] G protein-coupled chemoattractant receptors activate, recruit and capture G proteins for wide range chemotaxis2023

    • Author(s)
      Masahiro Ueda
    • Organizer
      19th International Conference on Retinal Proteins
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 自動1分子イメージング法の開発と創薬基盤技術への展開2023

    • Author(s)
      上田 昌宏
    • Organizer
      第128回日本解剖学会総会・全国学術集会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 1分子イメージングを用いた薬剤スクリーニング法の開発2022

    • Author(s)
      上田 昌宏
    • Organizer
      理研DMP創薬セミナー
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 細胞膜機能の1分子解析と創薬基盤技術への展開2022

    • Author(s)
      上田 昌宏
    • Organizer
      AMED脂質領域 公開シンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] ゆらぎの階層性と生命機能2022

    • Author(s)
      上田 昌宏
    • Organizer
      生命機能研究科 創立20周年記念シンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] 生物におけるゆらぎの階層性と情報統合ダイナミクス2022

    • Author(s)
      上田 昌宏
    • Organizer
      TMDU数理生物学セミナー
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 細胞の走化性に学ぶゆらぎの階層性2022

    • Author(s)
      上田 昌宏
    • Organizer
      物性研ワークショップ「開放系トポロジーと生体・量子・統計物理」
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] NBRP細胞性粘菌2022

    • Author(s)
      上村陽一郎,桑山秀一,上田 昌宏
    • Organizer
      第95回日本薬理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 細胞の自発運動制御シグナルの生成に関与するRasGEFs2022

    • Author(s)
      岩本浩司, 松岡里実, 上田昌宏
    • Organizer
      生体運動合同班会議 2022
    • Related Report
      2021 Annual Research Report
  • [Presentation] Comprehensive analysis of GEFs involved in the regulation of a Ras excitable system2021

    • Author(s)
      Koji Iwamoto, Satomi Matsuoka,Masahiro Ueda
    • Organizer
      第44回日本分子生物学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 走化性シグナル伝達における三量体Gタンパク質活性制御2021

    • Author(s)
      上村陽一郎,上田昌宏
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] NBRP「細胞性粘菌」:多様な研究分野で利用されるモデル生物2021

    • Author(s)
      上村陽一郎,桑山秀一,上田 昌宏
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 運動する細胞の前後極性形成のための自発シグナル生成メカニズム2021

    • Author(s)
      松岡里実,上田昌宏
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Subpopulation of chemotactic cells with extremely high sensitivity2021

    • Author(s)
      松岡里実,上田昌宏
    • Organizer
      第59回日本生物物理学会年会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Comprehensive analysis of GEFs involved in the regulation of a Ras excitable system2021

    • Author(s)
      Koji Iwamoto, Satomi Matsuoka,Masahiro Ueda
    • Organizer
      第59回日本生物物理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Large-scale analysis of receptor behaviors with automated single-molecule imaging system2021

    • Author(s)
      廣島通夫, 渡邉大介, 上田昌宏
    • Organizer
      第59回日本生物物理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 膜タンパク質分子の拡散運動の細胞膜フィールドモデルの構築2021

    • Author(s)
      竹林和俊,上村陽一郎,上田昌宏
    • Organizer
      日本細胞性粘菌学会第11回例会
    • Related Report
      2021 Annual Research Report
  • [Presentation] GEF による興奮系 Ras の制御機構の解明2021

    • Author(s)
      岩本浩司, 松岡里実, 上田昌宏
    • Organizer
      日本細胞性粘菌学会第11回例会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 走化性シグナル伝達における三量体Gタンパク質活性制御因子Gip1, Ric8の役割2021

    • Author(s)
      上村陽一郎,上田昌宏
    • Organizer
      日本細胞性粘菌学会第11回例会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 運動する細胞における前後極性の自己組織化2021

    • Author(s)
      松岡里実,上田昌宏
    • Organizer
      第13回光塾
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 自己組織化による細胞極性形成の1分子粒度シミュレーション2020

    • Author(s)
      松岡 里実, 上田 昌宏
    • Organizer
      第58回日本生物物理学会年会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 細胞内 1 分子イメージングの自動化と細胞内シグナル伝達への適用2020

    • Author(s)
      廣島 通夫, 上田 昌宏
    • Organizer
      CBI学会2020大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 走化性濃度勾配センシングにおける三量体Gタンパク質制御2020

    • Author(s)
      上村 陽一郎,上田 昌宏
    • Organizer
      生体運動研究合同班会議
    • Related Report
      2020 Annual Research Report
  • [Presentation] 細胞内自己組織化現象の1分子計測に基づく1分子粒度シミュレーション2020

    • Author(s)
      松岡 里実, 上田 昌宏
    • Organizer
      日本化学会第100春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 自己組織化による細胞極性形成の1分子粒度シミュレーション2020

    • Author(s)
      松岡 里実, 上田 昌宏
    • Organizer
      第58回日本生物物理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 自己組織化による細胞極性形成の1分子粒度シミュレーション2020

    • Author(s)
      松岡 里実, 上田 昌宏
    • Organizer
      第10回日本細胞性粘菌学会例会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 走化性勾配認識における三量体Gタンパク質制御2020

    • Author(s)
      上村 陽一郎,上田 昌宏
    • Organizer
      第10回日本細胞性粘菌学会例会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Identification of GEFs regulating the excitability of Ras in motile cells2020

    • Author(s)
      Koji Iwamoto, Satomi Matsuoka,Masahiro Ueda
    • Organizer
      第58回日本生物物理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Investigation of automatic single-molecule tracking method for large-scale single-molecule imaging analysis2020

    • Author(s)
      Sotaro Mori, Masato Yasui, Satomi Matsuoka, Masahiro Ueda
    • Organizer
      第58回日本生物物理学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 走化性細胞の濃度勾配認識におけるRGSの役割2020

    • Author(s)
      黒岩 麟平, 上村 陽一郎, 上田 昌宏
    • Organizer
      日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Roles of Gip1 and Ric8 in activation of heterotrimeric G proteins for eukaryotic chemotaxis2020

    • Author(s)
      上村 陽一郎,上田 昌宏
    • Organizer
      日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] From single molecules to cellular polarity: how anterior-posterior polarity in PI3-kinase and PTEN localization is self-organized2020

    • Author(s)
      Masahiro Ueda
    • Organizer
      Single Molecule Biophysics Les Houches 2020
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Automated single molecule imaging in living cells2019

    • Author(s)
      Masahiro Ueda
    • Organizer
      Quantitative Biology 2019: Dynamic Signaling in Cells and Embryos
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 細胞内1分子イメージング解析の自動化2019

    • Author(s)
      上田 昌宏
    • Organizer
      異分野融合による次世代光生物学研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Automated single-molecule imaging in living cells2019

    • Author(s)
      Masahiro Ueda
    • Organizer
      UCD-OU Joint Symposium ” Harnessing the Power of Biotechnology for Human and Planetary Health
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 細胞内1分子イメージング解析の自動化とシグナル伝達への適用2019

    • Author(s)
      上田 昌宏
    • Organizer
      日本顕微鏡学会 第62回シンポジウム 「AIを用いた顕微イメージングの将来」
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Automated single-cell and single-molecule imaging analysis2019

    • Author(s)
      Masahiro Ueda
    • Organizer
      The 42nd Annual Meeting of The Molecular Biology Society of Japan
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Remarks] 大阪大学大学院生命機能研究科1分子生物学研究室

    • URL

      https://www.fbs.osaka-u.ac.jp/ja/research_group/detail/2

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
  • [Remarks] 大阪大学大学院生命機能研究科1分子生物学研究室

    • URL

      https://www.fbs.osaka-u.ac.jp/labs/ueda/

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
  • [Remarks] 理化学研究所生命機能科学研究センター細胞シグナル動態研究チーム

    • URL

      http://www.qbic.riken.jp/csd/ja/index.html

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report 2021 Annual Research Report 2020 Annual Research Report 2019 Annual Research Report
  • [Remarks] Laboratory for Cell Signaling Dynamics, RIKEN BDR

    • URL

      https://www.bdr.riken.jp/en/research/labs/ueda-m/index.html

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
  • [Remarks] 命名“Dynamic partitioning” 膜タンパク質が局在化するしくみを解明

    • URL

      https://resou.osaka-u.ac.jp/ja/research/2024/20240110_2

    • Related Report
      2023 Annual Research Report
  • [Remarks] 大阪大学大学院生命機能研究科1分子生物学研究室

    • URL

      http://www.fbs.osaka-u.ac.jp/labs/ueda/

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report 2019 Annual Research Report
  • [Remarks] Laboratory for Cell Signaling Dynamics, RIKEN BDR

    • URL

      http://www.qbic.riken.jp/csd/en/index.html

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report 2019 Annual Research Report
  • [Remarks] 真核細胞の運動方向を決める分子基盤となる脂質-タンパク質相互作用を解明

    • URL

      http://www.fbs.osaka-u.ac.jp/jpn/events/achievement/ueda-20200228/

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 受容体型チロシンキナーゼの活性評価方法2024

    • Inventor(s)
      上田昌宏、廣島通夫、渡邉 大介
    • Industrial Property Rights Holder
      上田昌宏、廣島通夫、渡邉 大介
    • Industrial Property Rights Type
      特許
    • Filing Date
      2024
    • Related Report
      2023 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 1分子イメージングを用いた上皮成長因子受容体EGFRの活性評価に基づく薬剤スクリーニング法2023

    • Inventor(s)
      上田昌宏、廣島通夫、渡邉 大介
    • Industrial Property Rights Holder
      上田昌宏、廣島通夫、渡邉 大介
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2023-031358
    • Filing Date
      2023
    • Related Report
      2022 Annual Research Report
  • [Patent(Industrial Property Rights)] Autofocus device, and optical apparatus and microscope2021

    • Inventor(s)
      安井 真人,廣島 通夫,上田 昌宏
    • Industrial Property Rights Holder
      安井 真人,廣島 通夫,上田 昌宏
    • Industrial Property Rights Type
      特許
    • Filing Date
      2021
    • Related Report
      2020 Annual Research Report
    • Overseas

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Published: 2019-04-18   Modified: 2025-01-30  

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