| Budget Amount *help |
¥45,370,000 (Direct Cost: ¥34,900,000、Indirect Cost: ¥10,470,000)
Fiscal Year 2023: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2022: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2021: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2020: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Fiscal Year 2019: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
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| Outline of Final Research Achievements |
Neutron crystallographic analysis of a standard [NiFe]-hydrogenase in the oxidized form was successfully performed. The active site was found to contain a planar square Ni(II). A low-barrier hydrogen bond at the ligand (S) of the proximal Fe-S was found, suggesting that the Fe-S functions as an electron donor when O2 invades. Based on the crystal structure of the CO-bound form of the weakly oxygen-tolerant hydrogenase, a positive correlation between oxygen and CO tolerance was proposed.
FT-IR analysis of the oxidized NAD+-reducing [NiFe]-hydrogenase was successfully performed, and it was concluded that the Ni(IV) structure of the active site contributes to oxygen tolerance. X-ray analysis of the formate dehydrogenase from one of the plant symbiotic bacteria revealed its evolutionary relationship with other energy-metabolizing enzymes.
We also developed a Raman method to assay hydrogenases, and found that the enzyme is catalytically active mainly at the gas-liquid interface.
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