静水圧メカノシグナル制御とその組織工学への応用

• Project/Area Number: 19H01173
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Medium-sized Section 90:Biomedical engineering and related fields
• Research Institution: The University of Tokyo
• Principal Investigator: 牛田 多加志 東京大学, 大学院工学系研究科(工学部), 名誉教授 (50323522)
• Co-Investigator(Kenkyū-buntansha): 上田 太郎 早稲田大学, 理工学術院, 教授 (90356551) ; 古川 克子 東京大学, 大学院工学系研究科(工学部), 准教授 (90343144) ; 伊藤 弓弦 筑波大学, 生命環境系, 教授 (30500079) ; 佐藤 正人 東海大学, 医学部, 教授 (10056335)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥44,460,000 (Direct Cost: ¥34,200,000、Indirect Cost: ¥10,260,000); Fiscal Year 2022: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000); Fiscal Year 2021: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000); Fiscal Year 2020: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000); Fiscal Year 2019: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
• Keywords: 静水圧 / 軟骨細胞 / シグナル / メカノシグナル / 再生軟骨 / シグナル伝達

Outline of Research at the Start

軟骨細胞がいかに静水圧を受容するか，そのメカニズムについては依然不明のままである．本研究においては，そのメカニズムの解明のために再構築系，細胞実験系およびトランスクリトーム解析系の３つの系を用いて，軟骨細胞の静水圧メカノシグナル制御機構を分子レベルで検証する．さらに，本研究においては，軟骨細胞の静水圧メカノシグナル制御機構に基づいた最適な静水圧負荷技術を開発し， in vitro実験，動物移植実験を通じて，静水圧負荷の有効性を評価することより，組織工学への応用を目指した軟骨細胞分化制御および軟骨組織再生の基盤技術の開発を行う．

Outline of Annual Research Achievements

Rasの活性化をFRET強度の変化でモニターできるRaichuを用いて，Ras活性化の静水圧感受性の検出とメカニズム解明を目指した研究を進めた．これまでRaichuの単離精製とこれを用いたRas活性化の測定ができるようになっていたが，Raichu 内のCFPとYFPが弱く相互作用しこれが圧力感受性を示すことが判明した．そこでGFPの二量体化を阻害する点変異をRaichu内のCFPとYFPに導入したmRaichuを作成した。mRaichuは，Raichuに比してCFPとYFPの相互作用が弱く、Rasの圧力感受性についてのより定量的な測定が可能になった．
軟骨細胞に対し0 - 18 MPaの静水圧負荷下でリアルタイム観察を行い，阻害剤を用いることで上流のシグナル伝達機構の解明を目指した．結果として，静水圧の大きさに依存して相違が見られるような細胞内Ca2+濃度変化波形が得られた．さらに，阻害剤を投与した際の応答にも差が生じ，静水圧の大きさ依存的に機能する感知機構が異なる可能性や機能する感知機構の反応度合いが変化する可能性が示唆された．
軟骨細胞に関して静水圧処理群と無処理群を比較し，軟骨組織再生時にメカノシグナル負荷により亢進／抑制される因子を同定した．細胞内のみならずエクソソームも解析することで，軟骨組織内における誘導因子送達機構も含めたメカニズム解明を進めた．
これまでに日本白色家兎並びにラットを用いた異種同所性移植モデルによるin vivo有効性評価法を確立し，両動物種での修復再生効果の同等性を確認し，本研究における動物実験系をラットに決定した．移植方法および手技について検討を実施した．そして動物実験におけるポジティブコントロールとなる多指症由来軟骨組織より作製した細胞シートを用いて，静水圧負荷技術により作製された再生軟骨組織を用いた場合の移植方法や手技を確立した．

Research Progress Status

令和4年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和4年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] University of Helsinki(フィンランド)
• [Journal Article] Alternative Model to Animal Experiments by Reproducing the Physical Environment of Living Cartilage (2023)
  • Author(s): Minki Chang, Kevin Montagne, Yuma Omuro, Masashi Yasuno, Noriyasu Masumoto, Takashi Ushida, Katsuko S Furukawa
  • Journal Title: AATEX Volume: 28 Issue: 4 Pages: 1-7
  • DOI: 10.1111/aor.14713
  • Peer Reviewed
• [Journal Article] Intracellular calcium ion transients evoked by cell poking independently of released autocrine ATP in Madin-Darby canine kidney cells (2023)
  • Author(s): Minki Chang , Kevin Montagne , Katsuko S Furukawa , Takashi Ushida
  • Journal Title: Cell Biochem Funct Volume: 41 Issue: 7 Pages: 845-856
  • DOI: 10.1002/cbf.3834
  • Peer Reviewed
• [Journal Article] Simultaneous Hydrostatic and Compressive Loading System for Mimicking the Mechanical Environment of Living Cartilage Tissue (2023)
  • Author(s): Minki Chang, Yosuke Takahashi, Kyosuke Miyahira, Yuma Omuro, Kevin Montagne, Ryusei Yamada, Junki Gondo, Yu Kambe, Masashi Yasuno, Noriyasu Masumoto, Takashi Ushida, Katsuko S. Furukawa
  • Journal Title: micromachines Volume: 14 Issue: 8 Pages: 1632-1647
  • DOI: 10.3390/mi14081632
  • Peer Reviewed
• [Journal Article] microRNAs slow translating ribosomes to prevent protein misfolding in eukaryotes (2023)
  • Author(s): Sako Hiroaki、Youssef Mohieldin、Elisseeva Olga、Akimoto Takayuki、Suzuki Katsuhiko、Ushida Takashi、Yamamoto Tadashi
  • Journal Title: The EMBO Journal Volume: 42 Issue: 18
  • DOI: 10.15252/embj.2022112469
  • Peer Reviewed / Open Access
• [Journal Article] Effect of pressure conditions in uterine decellularization using hydrostatic pressure on structural protein preservation (2023)
  • Author(s): Dongzhe Wang, Narintadeach Charoensombut, Kinyoshi Kawabata, Tsuyoshi Kimura, Akio Kishida, Takashi Ushida, Katsuko S. Furukawa
  • Journal Title: Bioengineering Volume: 10 Issue: 7 Pages: 814-825
  • DOI: 10.3390/bioengineering10070814
  • Peer Reviewed
• [Journal Article] Cyclic stretch modulates cell morphology transition under geometrical confinement by covalently immobilized gelatin (2023)
  • Author(s): Kun Fang, Stefan Mueller, Motoki Ueda, Yasuhiro NAKAGAWA, Katsuko Furukawa, Takashi Ushida, Toshiyuki Ikoma and Yoshihiro Ito
  • Journal Title: Journal of Materials Chemistry B Volume: 11 Issue: 38 Pages: 9155-9162
  • DOI: 10.1039/d3tb00421j
  • Peer Reviewed
• [Journal Article] Polydactyly-derived allogeneic chondrocyte cell-sheet transplantation with high tibial osteotomy as regenerative therapy for knee osteoarthritis (2022)
  • Author(s): Hamahashi K, Toyoda E, Ishihara M, Mitani G, Takagaki T, Kaneshiro N, Maehara M, Takahashi T, Okada E, Watanabe A, Nakamura Y, Kato R, Matoba R, Takagi T, Akutsu H, Umezawa A, Kobayashi H, Akamatsu T, Yamato M, Okano T, Watanabe M, Sato M
  • Journal Title: npj Regenerative Medicine Volume: 7 Issue: 1
  • DOI: 10.1038/s41536-022-00272-1
  • Peer Reviewed / Open Access
• [Journal Article] Internal radial perfusion bioreactor promotes decellularization and recellularization of rat uterine tissue (2022)
  • Author(s): Charoensombut Narintadeach、Kawabata Kinyoshi、Kim Jeonghyun、Chang Minki、Kimura Tsuyoshi、Kishida Akio、Ushida Takashi、Furukawa Katsuko S.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 133 Issue: 1 Pages: 83-88
  • DOI: 10.1016/j.jbiosc.2021.09.007
  • NAID: 40022799393
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 変形性膝関節症に対する関節軟骨再生医療 自己・同種軟骨細胞シート移植の安全性と有効性について (2021)
  • Author(s): 浜橋恒介, 佐藤正人
  • Journal Title: 整形外科Surgical Technique Volume: 11(3) Pages: 117-121
  • NAID: 40022619868
  • Peer Reviewed
• [Journal Article] Candidates for Intra-Articular Administration Therapeutics and Therapies of Osteoarthritis (2021)
  • Author(s): Toyoda Eriko、Maehara Miki、Watanabe Masahiko、Sato Masato
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Issue: 7 Pages: 3594-3594
  • DOI: 10.3390/ijms22073594
  • Peer Reviewed / Open Access
• [Journal Article] Potential of Exosomes for Diagnosis and Treatment of Joint Disease: Towards a Point-of-Care Therapy for Osteoarthritis of the Knee (2021)
  • Author(s): Maehara Miki、Toyoda Eriko、Takahashi Takumi、Watanabe Masahiko、Sato Masato
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Issue: 5 Pages: 2666-2666
  • DOI: 10.3390/ijms22052666
  • Peer Reviewed / Open Access
• [Journal Article] Hydrostatic pressure prevents chondrocyte differentiation through heterochromatin remodeling (2020)
  • Author(s): Maki Koichiro、Nava Michele M.、Villeneuve Clementine、Chang Minki、Furukawa Katsuko S.、Ushida Takashi、Wickstrom Sara A.
  • Journal Title: Journal of Cell Science Volume: 134 Issue: 2 Pages: 247643-247643
  • DOI: 10.1242/jcs.247643
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 機械的刺激負荷により惹起された細胞内PLC-IP3シグナル応答のFRETを用いたリアルタイムイメージング (2020)
  • Author(s): 張 珉箕，林恵範，古川 克子，牛田多加志
  • Journal Title: 日本臨床バイオメカニクス学会誌 Volume: 41 Pages: 283-286
• [Journal Article] Osteogenic differentiation of murine mesenchymal stem cells by combination of surface topography and uniaxial stress (2020)
  • Author(s): JEONG Heonuk、YANG Xiaoguang、PEI Zihan、USHIDA Takashi、FURUKAWA Katsuko S.
  • Journal Title: Journal of Biomechanical Science and Engineering Volume: 15 Pages: 20-00009
  • NAID: 130007879754
  • Peer Reviewed
• [Journal Article] Suppressed osteogenic differentiation of murine mesenchymal stem cell by narrow microgrooves. (2020)
  • Author(s): Heonuk Jeong, Xiaoguang Yang, Takashi Ushida, Katsuko S Furukawa
  • Journal Title: Japanese Journal of Clinical Biomechanics Volume: 41 Pages: 293-298
  • NAID: 40022375385
  • Peer Reviewed
• [Journal Article] A mismatch between local chondrocyte death and mechanical properties of porcine articular cartilage exposed to injurious compression. (2020)
  • Author(s): Wenjing Huang, Hikaru Sasaki, Katsuko S Furukawa, and Takashi Ushida
  • Journal Title: Japanese Journal of Clinical Biomechanics Volume: 41 Pages: 305-312
  • NAID: 40022375428
  • Peer Reviewed
• [Journal Article] Acquired contractile ability in human endometrial stromal cells by passive loading of cyclic tensile stretch (2020)
  • Author(s): Kim Jeonghyun、Ushida Takashi、Montagne Kevin、Hirota Yasushi、Yoshino Osamu、Hiraoka Takehiro、Osuga Yutaka、Furuakwa Katsuko S.
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 9014-9014
  • DOI: 10.1038/s41598-020-65884-3
  • Peer Reviewed / Open Access
• [Journal Article] Regenerative effects of human chondrocyte sheets in a xenogeneic transplantation model using immune‐deficient rats (2020)
  • Author(s): Takizawa Daichi、Sato Masato、Okada Eri、Takahashi Takumi、Maehara Miki、Tominaga Ayako、Sogo Yasuyuki、Toyoda Eriko、Watanabe Masahiko
  • Journal Title: Journal of Tissue Engineering and Regenerative Medicine Volume: 14(9) Pages: 1296-1306
  • DOI: 10.1002/term.3101
  • Peer Reviewed / Open Access
• [Journal Article] Local Strain Distribution and Increased Intracellular Ca2+ Signaling in Bovine Articular Cartilage Exposed to Compressive Strain. (2020)
  • Author(s): Wenjing Huang, Minami Nagasaka, Katsuko S. Furukawa , Takashi Ushida
  • Journal Title: Journal of Biomechanical Engineering Volume: 142(6) Issue: 6
  • DOI: 10.1115/1.4045807
  • Peer Reviewed
• [Journal Article] Stretching of fibroblast cells on micropatterned gelatin on silicone elastomer (2020)
  • Author(s): Stefan Mueller, Motoki Ueda, Takashi Isoshima, Takashi Ushida, Yoshihiro Ito
  • Journal Title: Journal of Materials Chemistry B Volume: 8 Pages: 416-425
  • Peer Reviewed
• [Journal Article] K336I mutant actin alters the structure of neighbouring protomers in filaments and reduces affinity for actin-binding proteins (2019)
  • Author(s): Umeki N., Shibata K., Noguchi TQP, Hirose K., Sako Y., Uyeda TQP.
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 5353-5353
  • DOI: 10.1038/s41598-019-41795-w
  • Peer Reviewed / Open Access
• [Journal Article] Actin-binding domains mediate the distinct distribution of two Dictyostelium Talins through different affinities to specific subsets of actin filaments during directed cell migration (2019)
  • Author(s): Tsujioka, M., Uyeda, T.Q.P., Iwadate, Y., Patel, H., Shibata, K., Yumoto, T. and Yonemura, S.
  • Journal Title: PloS one Volume: 14 Issue: 4 Pages: e0214736-e0214736
  • DOI: 10.1371/journal.pone.0214736
  • NAID: 120006812012
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Multilineage-differentiating stress-enduring (Muse)-like cells exist in synovial tissue (2019)
  • Author(s): oyoda E, Sato M, Takahashi T, Maehara M, Nakamura Y, Mitani G, Takagaki T, Hamahashi K, Watanabe M
  • Journal Title: Regenerative Therapy Volume: 10 Pages: 17-26
  • Peer Reviewed
• [Journal Article] Transcriptomic and Proteomic Analyses Reveal the Potential Mode of Action of Chondrocyte Sheets in Hyaline Cartilage Regeneration. (2019)
  • Author(s): Nonaka K, Kawaguchi Y, Watanabe M
  • Journal Title: International Journal of Moleculer Sciences Volume: 21(1)
  • Peer Reviewed
• [Presentation] HP dissociates Ras-GAP complex and releasing from transient HP enhances Ras activation (2023)
  • Author(s): 松田瑛彦、Minki Chang, 古川克子、牛田多加志、上田太郎
  • Organizer: 第61回生物物理学会年会
• [Presentation] iPS細胞由来軟骨様組織と生体由来軟骨細胞から作製した硝子軟骨再生作用を示す細胞シートの共通発現タンパク質の同定 (2023)
  • Author(s): 豊田恵利子，佐藤正人，内山綾香，前原美樹 小川真梨奈, 田中竜実, 赤松正, 渡辺雅彦
  • Organizer: 第35回日本軟骨代謝学会
• [Presentation] 同種細胞シートの有効性関連因子の軟骨分化における機能解析 (2023)
  • Author(s): 豊田恵利子，佐藤正人，内山綾香，前原美樹 小川真梨奈, 田中竜実, 赤松正, 渡辺雅彦
  • Organizer: 第22回日本再生医療学会総会
• [Presentation] 異なる原料細胞から作製された軟骨細胞シートにおける細胞外小胞内miRNA 発現プロファイルの比較解析 (2023)
  • Author(s): 前原美樹、豊田恵利子、内山綾香、小川真、田中竜実、赤松正、渡辺雅彦、佐藤正人
  • Organizer: 第22回日本再生医療学会総会
• [Presentation] In vitro measurement of Ras activity under hydrostatic pressure (2022)
  • Author(s): 松田瑛彦、Minki Chang, 古川克子、牛田多加志、上田太郎
  • Organizer: 第34回 日本機械学会 バイオエンジニアリング講演会
• [Presentation] Ras activity measurement under hydrostatic pressure using improved Raichu (2022)
  • Author(s): Teruhiko Matsuda, Minki Chang, Katsuko Furukawa, Takashi Ushida, Taro QP Uyeda
  • Organizer: 第60回日本生物物理学会年会
• [Presentation] メカノバイオエンジニアリング技術による組織再生 (2021)
  • Author(s): 古川克子
  • Organizer: 第21回日本再生医療学会総会
• [Presentation] Establishment of in vitro system to measure Ras activity under hydrostatic pressure (2021)
  • Author(s): 松田 瑛彦, 張 箕, 古川 克子, 牛田 多加志, 上田 太郎
  • Organizer: 第59回日本生物物理学会年会
• [Presentation] Effect ofcombined stimulation of uniaxial compressive stress and hydrostatic pressure on a three-dimensional cartilage damage model (2021)
  • Author(s): Minki Chang, Yuma Omuro, Yosuke Takahashi, Kyosuke Miyahira, Noriyasu Masumoto, Masashi Yasuno, Takashi Ushida, Katsuko S. Furukawa
  • Organizer: The 11th Asian-Pacic Conference on Biomechanics
  • Int'l Joint Research
• [Presentation] 静水圧作用下における軟骨細胞のクロマチンリモデリングが細胞周期に及ぼす効果 (2021)
  • Author(s): 牧 功一郎, Nava Michele, Villeneuve Clementine, 張 箕, 古川 克子,牛田 多加志, Wickstrom Sara
  • Organizer: 日本機械学会第33回バイオエンジニアリング講演会
• [Presentation] 三次元軟骨損傷モデルに対する一軸圧縮応力と静水圧の複合刺激の効果 (2021)
  • Author(s): 張 箕, 大室祐磨, 高橋洋介, 宮平恭輔, 古川克子, 牛田多加志
  • Organizer: 日本機械学会第33回バイオエンジニアリング講演会
• [Presentation] 組織工学におけるDNAse Ⅰと高静水圧の相互作用の応用 (2021)
  • Author(s): Charoensombut Narintadeach,王東, Ushida Takashi, Furukawa Katsuko
  • Organizer: 日本機械学会第33回バイオエンジニアリング講演会
• [Presentation] Enzymatic high hydrostatic pressure for uterine tissue decellularization (2021)
  • Author(s): Narintadaech Charoensombut1, Dongzhe Wang, Kinyoshi Kawabata, Jeong Hyun Kim, Tsuyoshi Kimura, Akio Kishida, Takashi Ushida, Katsuko S. Furukawa
  • Organizer: TERMIS 6th World Congress
  • Int'l Joint Research
• [Presentation] Study of the effects of pressure conditions in high hydrostatic pressure uterine decellularization (2021)
  • Author(s): Dongzhe WANG, Narintadeach CHAROENSOMBUT, Tsuyoshi KIMURA, Akio KISHIDA, Takashi USHIDA, Katsuko FURUKAWA
  • Organizer: The 11th Asian-Pacic Conference on Biomechanics
  • Int'l Joint Research
• [Presentation] Cell surface markers expressed by autologous and allogeneic chondrocyte sheets. (2021)
  • Author(s): 1.Takahashi T, Uchiyama R, Okada E, Toyoda E, Maehara M, Kawaguchi Y, Morioka M, Yamashita A, Akamatsu T, Tsumaki N, Watanabe M, Sato M
  • Organizer: ISSCR/JSRM 2021 Tokyo International Symposium
  • Int'l Joint Research
• [Presentation] 細胞シートを用いた関節軟骨再生医療～先進医療までの道のりと今後の展開 (2021)
  • Author(s): 3.佐藤正人
  • Organizer: 第19回日本組織移植学会
  • Invited
• [Presentation] 細胞シート移植による変形性膝関節症の再生医療 (2021)
  • Author(s): 5.佐藤正人
  • Organizer: 第36回日本整形外科学会基礎学術集会
• [Presentation] 再生医療の現状とスポーツ医学への応用 (2021)
  • Author(s): 佐藤正人
  • Organizer: 第2回埼玉県健康スポーツ医会
  • Invited
• [Presentation] Cell Therapyは橋掛けと成り得るか？-早期OA介入としてのAPSの役割と可能性－. (2021)
  • Author(s): 佐藤正人
  • Organizer: 第一回日本Knee Osteotomy and Joint Preservation研究会
  • Invited
• [Presentation] 先進医療で実現する変形性膝関節症に対する軟骨細胞シート移植 (2021)
  • Author(s): 佐藤正人
  • Organizer: 第21回日本再生医療学会
• [Presentation] 細胞シートを用いた変形性膝関節症の再生医療 (2021)
  • Author(s): 佐藤正人
  • Organizer: 第21回日本再生医療学会
• [Presentation] Reducing pressure promotes in vivo regeneration of high hydrostatic pressure decellularized uterine (2021)
  • Author(s): 王東喆，Narintadeach Charoensombut，木村剛，岸田晶夫，牛田多加志，古川克子
  • Organizer: 第20回日本再生医療学会総会
• [Presentation] Enzymatic-high hydrostatic pressure for fabrication of decellularized esophagus and intestine scaffold (2021)
  • Author(s): Narintadeach Charoensombut，王東喆，木村剛，岸田晶夫，牛田多加志，古川克子
  • Organizer: 第20回日本再生医療学会総会
• [Presentation] Regenerative medicine for the treatment of osteoarthritis of the knee (2021)
  • Author(s): Sato M
  • Organizer: JAPAN HEALTHCARE UPDATE CONFERENCE
  • Int'l Joint Research
• [Presentation] PRP・APS療法はOA治療を変えるのか？ (2021)
  • Author(s): 佐藤正人.
  • Organizer: 第33回日本軟骨代謝学会
• [Presentation] Regenerative medicine through the transplantation of chondrocyte sheets for the treatment of osteoarthritis of the knee (2020)
  • Author(s): Sato M
  • Organizer: JOSKAS-JOSSM2020
  • Int'l Joint Research / Invited
• [Presentation] 膝関節軟骨修復に用いる多指症軟骨由来細胞の収集 (2020)
  • Author(s): 岡田恵里, 佐藤正人, 前原美樹, 豊田恵利子, 高橋匠, 渡部綾子, 白砂早織, 和才志帆, 赤松正, 渡辺雅彦.
  • Organizer: 第19回日本再生医療学会総会
• [Presentation] 運動器の再生医療 (2020)
  • Author(s): 佐藤正人
  • Organizer: 第19回日本再生医療学会総会
• [Presentation] 細胞シートを用いた変形性膝関節症に対する再生医療 (2020)
  • Author(s): 佐藤正人, 濱橋恒介, 三谷玄弥, 高垣智紀, 金城永俊, 芹ケ野健司, 赤松正, 渡辺雅彦
  • Organizer: 第93回日本整形外科学会学術総会
• [Presentation] リウマチ治療への再生医療の活用 (2020)
  • Author(s): 佐藤正人
  • Organizer: 第49回日本リウマチの外科学会
• [Presentation] Direction of the cooperative cluster formation of fimbrin along actin filaments (2019)
  • Author(s): Naoki Hosokawa, Masahiro Kuragano, Keitaro Shibata, Taro Q.P. Uyeda, Kiyotaka Tokuraku
  • Organizer: 第57回日本生物物理学会
• [Presentation] Self-regulatory mechanisms for the segregation of actin binding proteins on actin network (2019)
  • Author(s): Yosuke Yamazaki, Taro QP Uyeda
  • Organizer: 第57回日本生物物理学会
• [Presentation] Establishment of observation system to evaluate whether two different actin binding proteins bind to actin mutually or accommodative (2019)
  • Author(s): Tenji Yumoto, Taro QP Uyeda
  • Organizer: 第57回日本生物物理学会
• [Presentation] reatment of knee osteoarthritis with the APS Kit: the Japanese experience (2019)
  • Author(s): Sato M
  • Organizer: ICRS2019
  • Int'l Joint Research / Invited
• [Presentation] Evaluation of Allogeneic Chondrocytes Derived from Polydactyly Surgery for the Regenerative Treatment of Knee Joint Cartilage. (2019)
  • Author(s): Okada E, Sato M, Maehara M, Toyoda E, Takahashi T, Watanabe A, Shirasuna S, Wasai S, Akamatsu T, Watanabe M
  • Organizer: 7th Asian Biomaterials Congress (ABMC)
  • Int'l Joint Research
• [Presentation] Articular cartilage regeneration using polydactyly-derived chondrocyte sheets cryopreserved by vitrification method. (2019)
  • Author(s): Maehara M, Sato M, Toyoda E, Takahashi T, Okada E, Akamatsu T, Matsumura K, Suong-Hyu S, Nagashima H, Watanabe M
  • Organizer: Tissue Engineering and Regenerative Medicine International Society Asia Pacific Chapter (TERMIS-AP)
  • Int'l Joint Research
• [Book] 長引く膝の痛みに対する治療戦略, 膝周囲骨切り術と軟骨細胞シートによる変形性膝関節症治療の可能性 (2020)
  • Author(s): 浜橋恒介, 佐藤正人
  • Total Pages: 232
  • Publisher: 日本医事新報社
  • ISBN: 9784784957804