• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of energetics of biomolecular motor F1-ATPase from the perspective of physical plausibility

Research Project

Project/Area Number 19H01864
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 13040:Biophysics, chemical physics and soft matter physics-related
Research InstitutionTohoku University

Principal Investigator

Nakayama Yohei  東北大学, 工学研究科, 助教 (20757728)

Co-Investigator(Kenkyū-buntansha) 中村 壮伸  国立研究開発法人産業技術総合研究所, 材料・化学領域, 主任研究員 (10642324)
Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2023: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Keywords分子モーター / 非平衡熱力学 / 非平衡統計力学 / 生物物理
Outline of Research at the Start

生体分子モーターF1-ATPaseが持っているとされる、内部散逸が極めて小さいという性質を、反応座標の選び方によらない手法で一分子実験を行うことで検討し直す。
これまでの研究で、F1-ATPaseが回転する際に生じる熱を議論するために使われてきた関係式は、どのような変数に着目するかに応じて結果が変わってしまう、という意味で物理的に妥当でないことがわかっている。
顕微鏡観察によってF1-ATPaseの運動を一分子レベルで測定するときに、着目している変数がどのような性質を持っているかは一般には明らかではないので、この問題を解決した関係式を用いて、F1-ATPaseの内部散逸の測定を行う。

Outline of Final Research Achievements

A biomolecular motor F1-ATPase may operate not only as a molecular motor driven by the hydrolysis of adenosine triphosphate (ATP), but also as a part of ATP synthase. We clarified that these two operations are not simply the reverse processes of each other, but are qualitatively quite different. A key idea was to reconsider the physical validity of a formula used in a previous research. In addition, we elucidated mechanisms behind an asymmetry between the two operations.

Academic Significance and Societal Importance of the Research Achievements

明らかになったメカニズムは、分子モーター・ATP合成酵素のどちらかとして動作している場合のみに働く制御機構とみなせる。ATPは細胞内のエネルギー通貨と呼ばれる分子であるため、その合成を担うF1-ATPaseの制御は精密になされていると期待できる。そのため、今回得られた成果は他の生体分子モーターや人工分子モーターに制御機構を実装する上での指針になる、という意義がある。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (12 results)

All 2024 2023 2022 2021 2020

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (6 results) (of which Invited: 1 results)

  • [Journal Article] Derivation of the Invariant Free-Energy Landscape Based on Langevin Dynamics2024

    • Author(s)
      Nakamura Takenobu
    • Journal Title

      Physical Review Letters

      Volume: 132 Issue: 13 Pages: 137101-137101

    • DOI

      10.1103/physrevlett.132.137101

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optimal Rectification without Forward-Current Suppression by Biological Molecular Motor2021

    • Author(s)
      Nakayama Yohei、Toyabe Shoichi
    • Journal Title

      Physical Review Letters

      Volume: 126 Issue: 20 Pages: 208101-208101

    • DOI

      10.1103/physrevlett.126.208101

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stochastic order parameter dynamics for phase coexistence in heat conduction2021

    • Author(s)
      Sasa Shin-ichi、Nakagawa Naoko、Itami Masato、Nakayama Yohei
    • Journal Title

      Physical Review E

      Volume: 103 Issue: 6 Pages: 062129-062129

    • DOI

      10.1103/physreve.103.062129

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effective Langevin equations leading to large deviation function of time-averaged velocity for a nonequilibrium Rayleigh piston2021

    • Author(s)
      Itami Masato、Nakayama Yohei、Nakagawa Naoko、Sasa Shin-ichi
    • Journal Title

      Physical Review E

      Volume: 103 Issue: 2 Pages: 022125-022125

    • DOI

      10.1103/physreve.103.022125

    • NAID

      120007164841

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tight Chemomechanical Coupling of the F1 Motor Relies on Structural Stability2020

    • Author(s)
      Tanaka Mana、Kawakami Tomohiro、Okaniwa Tomoaki、Nakayama Yohei、Toyabe Shoichi、Ueno Hiroshi、Muneyuki Eiro
    • Journal Title

      Biophysical Journal

      Volume: 119 Issue: 1 Pages: 48-54

    • DOI

      10.1016/j.bpj.2020.04.039

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiplicative Langevin equation to reproduce long-time properties of nonequilibrium Brownian motion2020

    • Author(s)
      Seya Atsumasa、Aoyagi Tatsuya、Itami Masato、Nakayama Yohei、Nakagawa Naoko
    • Journal Title

      Journal of Statistical Mechanics: Theory and Experiment

      Volume: 2020 Issue: 1 Pages: 013201-013201

    • DOI

      10.1088/1742-5468/ab54bc

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] F1-ATPaseが示すATP合成/分解の間で非対称な基質濃度依存性のTASAMによる解析2023

    • Author(s)
      中山洋平
    • Organizer
      日本物理学会 2023年春季大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] F1-ATPaseの反応速度のATP分解/合成とで非対称な基質濃度依存性2022

    • Author(s)
      中山洋平,鳥谷部祥一
    • Organizer
      日本物理学会 第77回年次大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 生体分子モーターF1のATP合成という目的に合った設計原理2021

    • Author(s)
      中山洋平
    • Organizer
      日本物理学会 2021年秋季大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 自由エネルギーの浪費を抑えるF1-ATPaseの速度論的制御機構2020

    • Author(s)
      中山洋平,鳥谷部祥一
    • Organizer
      日本物理学会 2020年秋季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Kinetic ratchet mechanism of F1-ATPase selectively suppresses hydrolysis of ATP2020

    • Author(s)
      中山洋平,鳥谷部祥一
    • Organizer
      The 58th Annual Meeting of the BSJ
    • Related Report
      2020 Annual Research Report
  • [Presentation] F1_-ATPaseにおけるATP合成/分解の非対称性2020

    • Author(s)
      中山洋平, 鳥谷部祥一
    • Organizer
      日本物理学会 第75回年次大会
    • Related Report
      2019 Annual Research Report

URL: 

Published: 2019-04-18   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi