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Measurement of mechanical properties of cells by real and virtual coupled resonatorsl

Research Project

Project/Area Number 19H02090
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 20010:Mechanics and mechatronics-related
Research InstitutionUniversity of Tsukuba

Principal Investigator

Yabuno Hiroshi  筑波大学, システム情報系, 教授 (60241791)

Co-Investigator(Kenkyū-buntansha) 山本 泰之  国立研究開発法人産業技術総合研究所, 計量標準総合センター, 主任研究員 (00398637)
松本 壮平  国立研究開発法人産業技術総合研究所, エレクトロニクス・製造領域, 副研究部門長 (70358050)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2021: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Keywordsレゾネータ / 弾性計測 / 共振 / 自励振動 / モード局在化 / 弱連成 / レゾネーター / バーチャル / 計測 / 高感度計測 / カンチレバー / 非線形 / マイクロレゾネーター / 生体試料 / バーチャルレゾネーター / FPGA / 原子間力顕微鏡
Outline of Research at the Start

細胞の特性を知るための指標として、その硬さがある。硬さを調べる方法はさまざま存在するが、細胞など微小な測定物の弾性を精度よく計測することは、従来法の延長では不可能である。そこでお互いに力を及ぼし合う2つの同一の振動系を用意する。何も力が作用していない場合は、二つの振動系の振幅比は1:1である。これに対して、2つの内の一つの振動体に力が加わると、たとえそれが極めて小さい力であっても、振幅比が1:1から大きくずれる。この現象を使った、高感度な弾性計測システムを本研究では構築していく。

Outline of Final Research Achievements

In order to measure the elastic force of a micro object with ultra-high sensitivity, we proposed a virtual resonator method, actually measured the microstiffness, and confirmed its effectiveness. A resonator is brought into contact with the measurement target and its rigidity is measured. It is known that weakly coupled resonators of the same kind as this resonator improve sensitivity. In order to realize such a resonator, we proposed a virtual method (a method of constructing it in a computer rather than using an actual resonator), measured the atomic force that can simulate the elastic force, and confirmed its effectiveness.

Academic Significance and Societal Importance of the Research Achievements

本研究によって、微小な弾性力の計測法が構築された。弾性力とは物体の硬さを意味する物理量である。とくに生体試料の硬さを調べることは、がん細胞などを識別したり、iPS細胞の優劣の識別など、医学的な分野への貢献度が高く、新しい生体工学の発展に大きく寄与するものと考える。すなわち、これまで化学的に行われてきた細胞の識別が、物理的に行われ、計測試料に影響を与えずにそれが可能になる。さらに、弾性力は質量と並ぶ基本的な物理量であり、微小弾性力計測の方法を確立したことは、物理学全般にわたり、これまで不可能であった、物性の特徴づけに新たな展開を生み出すことになる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (11 results)

All 2022 2021 2020 2019

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Experimental amplitude and frequency control of a self-excited microcantilever by linear and nonlinear feedback2022

    • Author(s)
      Higuchi Eisuke、Yabuno Hiroshi、Yamamoto Yasuyuki、Matsumoto Sohei
    • Journal Title

      Journal of Micromechanics and Microengineering

      Volume: 32 Issue: 3 Pages: 034001-034001

    • DOI

      10.1088/1361-6439/ac4643

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Amplitude control for sensorless self-excited oscillation of cantilever based on a piezoelectric device2022

    • Author(s)
      Urasaki Shinpachiro、Yabuno Hiroshi
    • Journal Title

      Nonlinear Dynamics

      Volume: 108 Issue: 1 Pages: 15-25

    • DOI

      10.1007/s11071-021-07181-w

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Highly sensitive measurements of perturbations in stiffness of a resonator by virtual coupling with a virtual resonator2021

    • Author(s)
      Uemori Masamichi、Yabuno Hiroshi、Ymamamoto Yasuyuki、Matsumoto Sohei
    • Journal Title

      Nonlinear Dynamics

      Volume: 107 Issue: 2 Pages: 1755-1764

    • DOI

      10.1007/s11071-021-06790-9

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification method for backbone curve of cantilever beam using van der Pol-type self-excited oscillation2020

    • Author(s)
      Urasaki Shinpachiro、Yabuno Hiroshi
    • Journal Title

      Nonlinear Dynamics

      Volume: 103 Issue: 4 Pages: 3429-3442

    • DOI

      10.1007/s11071-020-05945-4

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measurement of added mass for an object oscillating in viscous fluids using nonlinear self-excited oscillations2020

    • Author(s)
      Yang Jiahao、Yabuno Hiroshi、Yanagisawa Naoki、Yamamoto Yasuyuki、Matsumoto Sohei
    • Journal Title

      Nonlinear Dynamics

      Volume: 102 Issue: 4 Pages: 1987-1996

    • DOI

      10.1007/s11071-020-06087-3

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ultra-Sensitive Minute Mass Sensing Using a Microcantilever Virtually Coupled with a Virtual Cantilever2020

    • Author(s)
      Kasai, Y., Yabuno. H., Yamamoto, Y., and Matsumoto, S.
    • Journal Title

      Sensors

      Volume: 20 Issue: 7 Pages: 1823-1823

    • DOI

      10.3390/s20071823

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Highly sensitive AFM using self-excited weakly coupled cantilevers2019

    • Author(s)
      Lin, Y, Yabuno, H., Liu, X, Yamamoto, Y., and Matsumoto, S.
    • Journal Title

      Applied Physics Letters

      Volume: 115 Issue: 13

    • DOI

      10.1063/1.5115836

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] Destabilized Mode in the Fluid Conveying Pipe Depending on the Bending Stiffness2021

    • Author(s)
      Eisuke Higuchi, Hiroshi Yabuno and Kiyotaka Yamashita
    • Organizer
      The ASME 2021 Virtual International Design Engineering Technical Conferences & Computers and Information in Engineering Conference
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nonlinear Viscometer Based on a Cantilever Self- Excited in Rayliegh-Type Oscillation2021

    • Author(s)
      Zhou Keyu, Yabuno Hiroshi
    • Organizer
      The ASME 2021 Virtual International Design Engineering Technical Conferences & Computers and Information in Engineering Conference
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] : Experimental Validity of Highly Sensitive Atomic Force Microscope (AFM)2019

    • Author(s)
      Yubo Lin, Hiroshi Yabuno, Xuan Liu, Yasuyuki Yamamoto, and Sohei Matsumoto
    • Organizer
      Dynamical Systems―Theory and Applications (DSTA 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] , “Ultrasensitive mass sensing using a single cantilever coupled with a computational cantilever2019

    • Author(s)
      Y. Kasai, H. Yabuno, T. Ishine, Y. Yamamoto, & S. Matsumoto
    • Organizer
      Dynamical Systems―Theory and Applications (DSTA 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research

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Published: 2019-04-18   Modified: 2023-01-30  

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