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Morphology controls and function emergence of self-organized biomolecular motor networks through controls of external environments

Research Project

Project/Area Number 19H02106
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 20020:Robotics and intelligent system-related
Research InstitutionGifu University

Principal Investigator

Nitta Takahiro  岐阜大学, 工学部, 准教授 (20402216)

Co-Investigator(Kenkyū-buntansha) 平塚 祐一  北陸先端科学技術大学院大学, 先端科学技術研究科, 准教授 (10431818)
森島 圭祐  大阪大学, 工学研究科, 教授 (60359114)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2020: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2019: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Keywords生体分子モーター / マイクロロボット / 自己組織化 / 機能発現
Outline of Research at the Start

細胞内の生体分子モーターと細胞骨格は置かれた環境に応じて様々なネットワーク形態をとることにより,多様な機能を実現している.この設計指針を応用することにより,少ない種類のパーツで環境に応じた多様な機能を発現するマイクロロボットの実現が期待される.本研究では,外部環境を制御し,生体分子モーターと細胞骨格のネットワーク形態を誘導することにより,マイクロロボットに必要な多様な機能を発現させる方法論の確立を目指す.このために,生体分子モーターと細胞骨格を様々な形状のマイクロウェルやマイクロ構造体と相互作用させ,ネットワーク形態を制御し機能発現を行う.

Outline of Final Research Achievements

Inspired by the principle which biological organisms use proteins and protein assemblies to build up higher order structures, the purpose of this study is to establish a methodology of inducing network morphology having designated emerging functions via external controls. To this end, experimental and computational studies were performed on pattern formations of networks consisting of biomolecular motors and cytoskeletal filaments confined in microfabricated chambers. Mechanical properties of microtubules, which are main components of the networks, were studied. In addition, to utilize the networks for electronics, microtubules were conjugated with gold nanoparticles.

Academic Significance and Societal Importance of the Research Achievements

本研究は,生体分子モーターを用いることにより高機能のマイクロロボットの開発を目指すものであり,実験およびシミュレーションによりこの可能性を探った.本研究で発展させた手法や得られた知見は,今後の細胞骨格と生体分子モーターからなるネットワークをマイクロロボットに導入する際に有益であるとともに,マイクロロボットの高機能化に資するものであると考える.

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (11 results)

All 2022 2021 2020 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Int'l Joint Research] McGill University(カナダ)

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Linking path and filament persistence lengths of microtubules gliding over kinesin2022

    • Author(s)
      Sweet May、Kang’iri Samuel Macharia、Nitta Takahiro
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 3081-3081

    • DOI

      10.1038/s41598-022-06941-x

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of defective motors on the active transport in biosensors powered by biomolecular motors2022

    • Author(s)
      Kang'iri Samuel Macharia、Salem Andrew、Nicolau Dan V.、Nitta Takahiro
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 203 Pages: 114011-114011

    • DOI

      10.1016/j.bios.2022.114011

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Motility resilience of molecular shuttles against defective motors2022

    • Author(s)
      Kang'iri Samuel Macharia、Nitta Takahiro
    • Journal Title

      IEEE Transactions on NanoBioscience

      Volume: 印刷中 Issue: 3 Pages: 439-444

    • DOI

      10.1109/tnb.2022.3170562

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Mathematical Model Predicting Gliding Speed of Actin Molecular Shuttles Over Myosin Motors in the Presence of Defective Motors2021

    • Author(s)
      Kang’iri Samuel Macharia、Nitta Takahiro
    • Journal Title

      Lecture Notes of the Institute for Computer Sciences, Social Informatics and Telecommunications Engineering

      Volume: 403 Pages: 207-214

    • DOI

      10.1007/978-3-030-92163-7_17

    • ISBN
      9783030921620, 9783030921637
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detachment of Microtubules Driven by Kinesin Motors from Track Surfaces Under External Force2021

    • Author(s)
      Sweet May、Nitta Takahiro
    • Journal Title

      Lecture Notes of the Institute for Computer Sciences, Social Informatics and Telecommunications Engineering

      Volume: 403 Pages: 199-206

    • DOI

      10.1007/978-3-030-92163-7_16

    • ISBN
      9783030921620, 9783030921637
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Presentation] Analyzing a printable actuator composed of an engineered kinesins by a computer simulation2021

    • Author(s)
      Yurino Aoyama, Yuichi Hiratsuka,Takahiro Nitta
    • Organizer
      日本生物物理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] A Mathematical Model Predicting Gliding Speed of Actin Molecular Shuttles Over Myosin Motors in the Presence of Defective Motors2021

    • Author(s)
      Kang’iri Samuel Macharia、Nitta Takahiro
    • Organizer
      BICT2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Detachment of Microtubules Driven by Kinesin Motors from Track Surfaces Under External Force2021

    • Author(s)
      Sweet May、Nitta Takahiro
    • Organizer
      BICT2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生体分子モーターの工学利用:バイオセンサー,人工筋肉2020

    • Author(s)
      新田高洋
    • Organizer
      第32回「電磁力関連のダイナミクス」シンポジウム(SEAD32)
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Computer simulation of printable artificial muscles composed of engineered kinesins and microtubules2020

    • Author(s)
      Yurino Aoyama, Yuichi Hiratsuka, Takahiro Nitta
    • Organizer
      日本生物物理学会第58回年会
    • Related Report
      2020 Annual Research Report

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Published: 2019-04-18   Modified: 2023-01-30  

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