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Quantitative Meta-barcoding for Rapid and Accurate Assessment of Species Diversity: Application to Community and eDNA samples

Research Project

Project/Area Number 19H02276
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 22060:Environmental systems for civil engineering-related
Research InstitutionEhime University

Principal Investigator

Watanabe Kozo  愛媛大学, 沿岸環境科学研究センター, 教授 (80634435)

Co-Investigator(Kenkyū-buntansha) 加藤 幹男  大阪府立大学, 高等教育推進機構, 教授 (30204499)
竹門 康弘  京都大学, 防災研究所, 准教授 (50222104)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2021: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2020: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2019: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Keywords河川 / 生物多様性 / メタバーコーディング / 底生動物 / 環境DNA
Outline of Research at the Start

次世代シーケンシングを活用したDNAメタバーコーディングにより,河川底生動物の種多様性を正確かつ迅速に評価する新規的技術を開発する。研究目的は以下3つである。
1)DNAメタバーコーディングの正確性を向上させるため,DNAデータベース上の登録種数やDNAマーカー数を迅速に増やす次世代シーケンシングに基づく手法を開発する。
2)従来は困難とされていた種ごとの個体数や湿重量の定量評価を可能にする定量DNAメタバーコーディング法を開発する。
3)「河川水が伏流する砂州上流端の河床には多様な種の環境DNAが濃縮される」という仮説を検証し,砂州フィルタリング機能を活用した環境DNAの高効率回収技術を開発する。

Outline of Final Research Achievements

We explored a method to accurately and rapidly assess the species diversity of riverine benthic fauna by DNA metabarcoding utilizing next-generation sequencing, and confirmed that DNA metabarcoding can be used to assess the relative abundance and wet weight of each species. We tested the hypothesis that environmental DNA of a variety of species is enriched in the riverbed at the upstream end of a gravel bar where river water is submerged, and found no significant differences in species diversity in the porewater at the upstream end of the gravel bar, the downstream end, and the intermediate sites. The species detected at these three sites also differed significantly.

Academic Significance and Societal Importance of the Research Achievements

安価・迅速・正確な生態系モニタリングを実現させるために必要なDNAメタバーコーディング法の提案に繋がる重要な発見をいくつか実現した。DNAメタバーコーディングに必要なDNAデータベースの拡充にも大きく貢献した。河川水から抽出した環境DNAと採取した群集から抽出したDNAの違いや類似性を明確にすることで、今後のDNAメタバーコーディングを活用した生態系モニタリングのあるべき方向性を示すことができた。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (51 results)

All 2022 2021 2020 2019

All Journal Article (25 results) (of which Int'l Joint Research: 19 results,  Peer Reviewed: 24 results,  Open Access: 23 results) Presentation (26 results) (of which Int'l Joint Research: 19 results,  Invited: 4 results)

  • [Journal Article] Spatially Varying Trophic Effects of Reservoir-Derived Plankton on Stream Macroinvertebrates Among Heterogeneous Habitats Within Reaches2022

    • Author(s)
      Shinji Takahashi, Yasuhiro Takemon, Tatsuo Omura, Kozo Watanabe
    • Journal Title

      Hydrobiologia

      Volume: accepted

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Implications of taxonomic and numerical resolution on DNA metabarcoding-based inference of benthic macroinvertebrate responses to river restoration2022

    • Author(s)
      Joeselle M. Serrana, Bin Li, Tetsuya Sumi, Yasuhiro Takemon, Kozo Watanabe
    • Journal Title

      Ecological Indicators

      Volume: 135 Pages: 108508-108508

    • DOI

      10.1016/j.ecolind.2021.108508

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Notes on the Taxonomic Status and Distribution of Some Cylindrotomidae (Tipuloidea, Diptera), with Emphasis on Japanese Species2022

    • Author(s)
      Levente-Peter Kolcsar, Nikolai Paramonov, Yume Imada, Daichi Kato, Maribet Gamboa, Dai Shinoka, Makoto Kato, Kozo Watanabe
    • Journal Title

      Zookeys

      Volume: 1083 Pages: 13-88

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Sediment-associated Microbial Community Profiling: Sample Pre-processing through Sequential Membrane Filtration for 16s rDNA Amplicon Sequencing2022

    • Author(s)
      Joeselle M. Serrana, Kozo Watanabe
    • Journal Title

      BMC Microbiology

      Volume: 22(1) Issue: 1 Pages: 33-33

    • DOI

      10.1186/s12866-022-02441-0

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Loss of a larval generic character: an interesting and new description for Isoperla vevcianensis Ikonomov, 1980 (Plecoptera: Perlodidae) with updated adult characters2021

    • Author(s)
      D?VID MUR?NYI, TIBOR KOV?CS, MARIBET GAMBOA, KOZO WATANABE
    • Journal Title

      Zootaxa

      Volume: 5082(6) Issue: 6 Pages: 541-552

    • DOI

      10.11646/zootaxa.5082.6.2

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Profiling the Microbial Community Structure and Functional Diversity of a Dam-regulated River Undergoing Gravel Bar Restoration2021

    • Author(s)
      Joeselle M. Serrana, Bin Li, Tetsuya Sumi, Yasuhiro Takemon, Kozo Watanabe
    • Journal Title

      Freshwater Biology

      Volume: 66 (11) Issue: 11 Pages: 2170-2184

    • DOI

      10.1111/fwb.13824

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Carotenoid Coloration and Coloration-Linked Gene Expression in Red Nile Tilapia (Oreochromis niloticus) Tissues2021

    • Author(s)
      Cruz, K. G. J., E. P. Landingin, M. B. Gajeton, S. I. D. Fernando, and K. Watanabe
    • Journal Title

      BMC Veterinary Research

      Volume: 17 Issue: 1 Pages: 314-314

    • DOI

      10.1186/s12917-021-03006-5

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Contribution to the Knowledge of Limoniidae (Diptera: Tipuloidea): First Records of 244 Species from Various European Countries2021

    • Author(s)
      Kolcsar L-P, P. Oosterbroek, D. I. Gavryushin, K. M. Olsen, N. M. Paramonov, V. Pilipenko, J. Stary, A. Polevoi, E. Eiroa, M. Andersson, J. Salmela, C. Quindroit, M. C. dOliveira, E. G. Hancock, J. Mederos, P. Boardman, E. Viitanen and K. Watanabe
    • Journal Title

      Biodiversity Data Journal

      Volume: 9

    • DOI

      10.3897/bdj.9.e67085

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Biofilm Inhibition in Candida albicans by Biologically-synthesized Gold Nanoparticles2021

    • Author(s)
      Cruz, K. G. J., E. D. Alfonso, S. I. D. Fernando, and K. Watanabe
    • Journal Title

      Frontiers in Microbiology

      Volume: 12 Pages: 665113-665113

    • DOI

      10.3389/fmicb.2021.665113

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Detailed Description and Illustration of Larva, Pupa, and Imago of Holorusia mikado (Westwood, 1876) (Diptera: Tipulidae) from Japan2021

    • Author(s)
      Kolcsar, L-P, D. T. Nakamura, Kato, and K. Watanabe
    • Journal Title

      Biodiversity Data Journal

      Volume: 9

    • DOI

      10.3897/bdj.9.e58009

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Simulation Modeling Reveals the Evolutionary Role of Landscape Shape and Species Dispersal on Genetic Variation within a Metapopulation2020

    • Author(s)
      Chiu, M-C, K. Nukazawa, T. M. Carvajal, V. H. Resh, B. Li, and K. Watanabe
    • Journal Title

      Ecography

      Volume: 43 (12) Issue: 12 Pages: 1891-1901

    • DOI

      10.1111/ecog.05118

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Branching Networks Can Have Opposing Influences on Genetic Variation in Riverine Metapopulations2020

    • Author(s)
      Chiu, M-C, B. Li, K. Nukazawa, V. H. Resh, T. M. Carvajal, and K. Watanabe
    • Journal Title

      Diversity and Distributions

      Volume: 26 (12) Issue: 12 Pages: 1813-1824

    • DOI

      10.1111/ddi.13160

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Revision of Japanese species of Nipponomyia Alexander, 1924 (Diptera, Pediciidae)2020

    • Author(s)
      Kolcsar, L. P., D. Kato, M. Gamboa, and K. Watanabe
    • Journal Title

      ZooKeys

      Volume: 1000 Pages: 71-105

    • DOI

      10.3897/zookeys.1000.55021

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Spatial Genetic Structure of the Invasive Riparian Tree Robinia pseudoacacia to Determine Migration Patterns to Inform Best Practices for Riparian Restoration2020

    • Author(s)
      Yaegashi, S., T. Omura, and K. Watanabe
    • Journal Title

      AoB PLANTS

      Volume: 12(5) Issue: 5

    • DOI

      10.1093/aobpla/plaa043

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Synopsis of the genus Ulomorpha Osten Sacken, 1869 (Diptera, Limoniidae) in Japan2020

    • Author(s)
      Kato, D., K. Watanabe, and L-P Kolcs?r
    • Journal Title

      ZooKeys

      Volume: 999 Pages: 147-163

    • DOI

      10.3897/zookeys.999.52831

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Remarkable Anoxia Tolerance by Stoneflies from a Floodplain Aquifer2020

    • Author(s)
      Malison, R. L., B. K. Ellis, A. G. DelVecchia, H. N. Jacobson, B. K. Hand, G. Luikart, H. A. Woods, M. Gamboa, K. Watanabe, and J. A. Stanford
    • Journal Title

      Ecology

      Volume: 101 (10) Issue: 10

    • DOI

      10.1002/ecy.3127

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Detection of Adaptive Divergence in Populations of the Stream Mayfly Ephemera strigata with Machine Learning2020

    • Author(s)
      Li, B., S. Yaegashi, T. M. Carvajal, M. Gamboa and K. Watanabe
    • Journal Title

      Ecology and Evolution

      Volume: 10 (13) Issue: 13 Pages: 6677-6687

    • DOI

      10.1002/ece3.6398

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Quorum Sensing-linked agrA Expression by Ethno-synthesized Gold Nanoparticles in Tilapia Streptococcus agalactiae Biofilm Formation2020

    • Author(s)
      Fernando, S. I. D., K. G. J. Cruz and K. Watanabe
    • Journal Title

      BioNanoScience

      Volume: 10 (3) Issue: 3 Pages: 696-704

    • DOI

      10.1007/s12668-020-00758-6

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A Remarkable New Genus and Species of Nemourinae (Plecoptera, Nemouridae) from Sichuan, China, with Systematic Notes on the Related Genera2020

    • Author(s)
      Mo, R-R., M Gamboa, K. Watanabe, G-Q Wang, W-H Li, D Yang, D Mur?nyi
    • Journal Title

      PLoS ONE

      Volume: 15(3) Issue: 3 Pages: e0229120-e0229120

    • DOI

      10.1371/journal.pone.0229120

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A New Species of Protonemura Kempny, 1898 (Plecoptera: Nemouridae) from the Ishizuchi Range, Shikoku, Japan2020

    • Author(s)
      Mur?nyi, D., M. Gamboa, and K. Watanabe
    • Journal Title

      Zootaxa

      Volume: 4718 (1) Issue: 1 Pages: 57-66

    • DOI

      10.11646/zootaxa.4718.1.4

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Understanding Hurdles in the Implementation of Health Information System (HIS) Through an Institutional Framework: A Case of Bandung, West Java Province, Indonesia2020

    • Author(s)
      Faridah, L, F. Rinawan, N. Fauziah, W. Mayasari, A. Dwiartama, K. Watanabe
    • Journal Title

      International Journal of Environmental Research and Public Health

      Volume: 17(5) Issue: 5 Pages: 1795-1795

    • DOI

      10.3390/ijerph17051795

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Are bacteria potential sources of fish environmental DNA ?2020

    • Author(s)
      Nukazawa K., Akahoshi K., Suzuki Y.
    • Journal Title

      PLoS ONE

      Volume: 15 Issue: 3 Pages: e0230174-e0230174

    • DOI

      10.1371/journal.pone.0230174

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Unraveling the Genetic Structure of the Coconut Scale Insect Pest (Aspidiotus rigidus Reyne) Outbreak Populations in the Philippines2019

    • Author(s)
      Serrana, J. M., N. Ishitani, T. M. Carvajal, B. J. M. Almarinez, A. T. Barrion, D. M. Amalin, K. Watanabe
    • Journal Title

      Insects

      Volume: 10 (11) Issue: 11 Pages: 374-374

    • DOI

      10.3390/insects10110374

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Spatial and Phylogenetic Structure of DNA-species of Alpine Stonefly Community Assemblages across Seven Habitats2019

    • Author(s)
      Gamboa, M., J. Serrana, Y. Takemon, M. T. Monaghan, K. Watanabe
    • Journal Title

      bioRxiv

      Volume: -

    • DOI

      10.1101/765578

    • Related Report
      2019 Annual Research Report
    • Open Access
  • [Journal Article] Comparison of DNA Metabarcoding and Morphological Identification for Stream Macroinvertebrate Biodiversity Assessment and Monitoring2019

    • Author(s)
      Serrana, J. M., Y. Miyake, M. Gamboa and K. Watanabe
    • Journal Title

      Ecological Indicators

      Volume: 101 Pages: 963-972

    • DOI

      10.1016/j.ecolind.2019.02.008

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 高見川(奈良県)で採集した水生昆虫の次世代シーケンサーによる種判別2022

    • Author(s)
      稲井公二・脇村圭・渡辺幸三・加藤幹男
    • Organizer
      日本陸水学会近畿支部会第32回研究発表会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Phylogeny and taxonomic revision of Palearctic Cylindrotomidae (Diptera)2021

    • Author(s)
      Levente-Peter Kolcsar, Yume Imada, Kozo Watanabe
    • Organizer
      Hungarian Academy of Science: Zootaxonomy - A Memorial Conference of Laszlo Papp
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 吉野川(奈良県)で採集した水生昆虫のメタバーコーディング解析2021

    • Author(s)
      稲井公二・脇村圭・渡辺幸三・加藤幹男
    • Organizer
      日本陸水学会第85回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Haplotype-level DNA metabarcoding from freshwater macroinvertebrate community samples2021

    • Author(s)
      Joeselle M. Serrana and Kozo Watanabe
    • Organizer
      1st DNAQUA International Conference: Biotic Indices & Metrics - New trends in bioassessment of aquatic ecosystems: from organisms to DNA-based metrics
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Taxonomic Revision of Japanese Snow Crane Flies (Chionea, limoniidae): A First Step to Understand the Effect of Climate Change on Winter Active Insect2021

    • Author(s)
      Kolcsar L.P., Kato D. Watanabe K.
    • Organizer
      5th International Conference on Climate Change 2021
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Contrasting Adaptive Genetic Consequences of Stream Insects under Changing Climate2021

    • Author(s)
      Nukazawa K., Chiu M-C., Kazama S., Watanabe K.
    • Organizer
      5th International Conference on Climate Change 2021 (ICCC 2021)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Elevational Patterns of Genetic Diversity in Riverine Populations in Japan2021

    • Author(s)
      Chiu M. C, Watanabe K
    • Organizer
      5th International Conference on Climate Change 2021 (ICCC 2021)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Taxonomic Revision of Japanese Snow Crane Flies (Chionea, limoniidae): A First Step to Understand the Effect of Climate Change on Winter Active Insects2021

    • Author(s)
      Kolcsar L.-P, Kato D., Watanabe K.
    • Organizer
      5th International Conference on Climate Change 2021 (ICCC 2021)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 高見川(奈良県)で採取した水生昆虫の次世代シーケンサーによる種判別2021

    • Author(s)
      稲井公二,脇村圭,渡辺幸三,加藤幹男
    • Organizer
      陸水学会近畿支部会第32回研究発表
    • Related Report
      2020 Annual Research Report
  • [Presentation] Molecular Genetic Approaches to the Assessment of Dam Impact on Stream Macroinvertebrates and Dengue Virus Surveillance of Vector Mosquitoes2020

    • Author(s)
      Kozo Watanabe
    • Organizer
      47th Philippine Society of Biochemistry and Molecular Biology (PSBMB) Annual Convention
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Sediment-associated microbial community profiling: sample pre-processing through sequential membrane filtration for 16s rDNA amplicon sequencing2020

    • Author(s)
      Joeselle M. Serrana and Kozo Watanabe
    • Organizer
      Joint Meeting of the 3rd Annual Meeting of The eDNA Society and the 36th Annual Meeting of the Society of Population Ecology (Online)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Recurrent seasonal dynamics of the planktonic eukaryotic communities in the polymictic eutrophic Lake M?ggelsee (Germany).2020

    • Author(s)
      Joeselle M. Serrana, Kingsly Chuo Beng, Jusytna Wolinska, Kozo Watanabe and Michael T. Monaghan
    • Organizer
      Water and Environment Technology Conference 2020 Online (WET2020-Online)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microbial community diversity across hyporheic zoes of gravel bars: taxonomic and fucntio nal metrics2020

    • Author(s)
      Arnelyn Doloiras-Larano,Maribet Gamboa, Shinji Takahashi, Joeselle Serrana Yasuhiro Tekemon, Paul R. Johnston, Michael T. Monaghan, and Kozo Watanabe
    • Organizer
      Water and Environment Technology Conference 2020 Online (WET2020-Online)
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular Approaches to the Study of Freshwater Biodiversity: Genomic Adaptation and DNA Metabarcoding.2019

    • Author(s)
      Kozo Watanabe
    • Organizer
      International Workshop of Water Quality Monitoring
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Landscape shape predicts enclosed genetic variation within the metapopulation of a species with varied dispersal2019

    • Author(s)
      Ming-Chih Chiu, Kozo Watanabe
    • Organizer
      International Workshop of Water Quality Monitoring
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Sediment Microbial Diversity and Community Composition of Restored Gravel Bars in the Trinity River, California2019

    • Author(s)
      Joeselle Serrana, Bin Li, Tetsuya Sumi, Yasuhiro Takemon, Kozo Watanabe
    • Organizer
      Philippine Society for Microbiology 48th Annual Convention
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Spatial and Phylogenetic Structure of DNA-species of Alpine Stoneflies Community Assemblages across Seven Habitats2019

    • Author(s)
      Maribet Gamboa, Joeselle Serrana, Michael Monaghan, Yasuhiro Takemon, Kozo Watanabe
    • Organizer
      Water and Environment Technology Conference 2019 (WET2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genome-wide Transcriptome Analysis of stream stonefly along a climatic gradient in Japana2019

    • Author(s)
      Arnelyn Doloiras-Larano, Gotoh Yusuke, Maribet Gamboa and Kozo Watanabe
    • Organizer
      Water and Environment Technology Conference 2019(WET(2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comparison of DNA metabarcoding and morphological identification for stream macroinvertebrate biodiversity assessment and monitoring2019

    • Author(s)
      Joeselle Serrana, Yo Miyake, Maribet Gamboa, Kozo Watanabe
    • Organizer
      Pest Management Council of the Philippines 51st Anniversary and Annual Scientific Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Protein quantitative traits loci (pQTL) expression of the stonefly Nemoura ovocercia (Plecoptera) along nationwide region of Japan2019

    • Author(s)
      Maribet Gamboa, Maria Claret Tsuchiya, Shunsuke Yamano, Thaddeus Carvajal, Hisato Iwata, Kozo Watanabe
    • Organizer
      Evolution 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Species dispersal mediates opposing influences of a branching network on genetic variation in a metapopulation2019

    • Author(s)
      Ming-Chih Chiu, Bin Li, Kei Nukazawa, Thaddeus Carvajal, Kozo Watanabe
    • Organizer
      2019 Annual Meeting of the Society for Freshwater Science
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dynamics of stream environmental DNA focusing on transport, bacteria and particle size2019

    • Author(s)
      Nukazawa K., Akahoshi K., Suzuki Y.
    • Organizer
      Asia Oceania Geosciences Society (AOGS) 16th Annual Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 環境DNAバーコーディング解析を用いた河川水生昆虫の種多様性の解明:高知県四万十川を例として2019

    • Author(s)
      八重樫咲子,細川大樹,渡辺幸三
    • Organizer
      応用生態工学会第23回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 四万十川と仁淀川の環境DNA及び底生動物群集のメタバーコーディング解析2019

    • Author(s)
      石谷直渡,八重樫咲子,Serrana, J.M.,Fernando, S.I.,渡辺幸三
    • Organizer
      応用生態工学会第23回大会
    • Related Report
      2019 Annual Research Report
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Published: 2019-04-18   Modified: 2023-01-30  

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