| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2021: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Outline of Final Research Achievements |
In this study, we developed a new metabolic engineering technology called PMPE (Parallel Metabolic Pathway Engineering). We used glucose and xylose as carbon sources for the production of target substances and cell growth, respectively, and designed the system so that the carbon derived from each sugar does not intersect within the metabolic system. After further examination of the culture medium and optimization of pH, MA was produced at 4.26 g/L after 80 hours of incubation, and the MA yield relative to consumed glucose reached 0.31 g/g after 80 hours of incubation. This yield was the highest in the world for MA production in batch operation, indicating that PMPE is an effective strategy.
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