Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2023: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2021: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2020: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2019: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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Outline of Final Research Achievements |
The cDNAs of Spider(Araneus ventricosus) dragline silk protein, MaSp1, MaSp2, and MaSp3, were cloned and sequenced. Each were 3-9 kbp in length, which were close to their full length. Furthermore, we established a genetically modified silkworm in which these genes are expressed as fusion proteins with the silkworm fibroin H chain. Mechanical properties of those genetically modified silkworm cocoon silks result in most of the strains showed the same or lower breaking strength as that of non-recombinant cocoon silks. The introduction of these spider silk proteins did not improve the mechanical properties of silk.
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