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Development of a microvascular circulation cultivation system and its application as an in vitro cancer simulator.

Research Project

Project/Area Number 19H02530
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 27040:Biofunction and bioprocess engineering-related
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Shinji Sugiura  国立研究開発法人産業技術総合研究所, 生命工学領域, 副連携研究室長 (10399496)

Co-Investigator(Kenkyū-buntansha) 松阪 諭  筑波大学, 医学医療系, 教授 (00372665)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2022: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2021: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Keywords血管 / 組織工学 / 生体模倣システム / 灌流培養 / マイクロ流体デバイス / 癌 / 相互作用 / Organ-on-a-chip / マイクロフルイディクス
Outline of Research at the Start

本研究では、我々が開発した圧力駆動型循環培養システムをベースとしてンプレスの微小血管組織循環培養系を構築することで、微小血管組織を生理学的な流れ刺激下で培養するとともに、癌の血管新生および浸潤評価へと応用する。将来的には、本研究で発する微小血管組織循環培養システムを癌のインビトロシミュレータとして病態解析や創薬に広く普及させることを目指す。

Outline of Final Research Achievements

This study proposed a new method for cultivating microvascular tissues in vitro using a pressure-driven biomimetic system (PD-MPS). By using the PD-MPS, blood vessels can be formed while the hydrogel is penetrated with the cultivation medium using air pressure. Human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) were embedded in fibrin gel and introduced into the PD-MPS. Perfusion of the cultivation solution at a pressure of 2 kPa resulted in spontaneous formation of blood vessels within the fibrin gel. An increase in flow rate was observed with the formation of the blood vessels. Blood vessel formation was not observed when cultivation was conducted without perfusion. These results suggest that the PD-MPS is a simpler and more effective method for promoting blood vessel formation in comparison to traditional methods.

Academic Significance and Societal Importance of the Research Achievements

この研究は、圧力駆動型の生体模倣システム(PD-MPS)を使用して微小血管組織の生体外培養において新たな方法を提案した。この手法は、血管組織が確実に形成された後に灌流を行わないと、血管組織が破壊される恐れのある従来の方法よりも簡便かつ効果的であることが示された。この手法は、将来的に血液脳関門モデルやオルガノイド培養、がんの転移試験等への利用が期待される微小血管の生体外培養系の開発に貢献することができる。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (4 results)

All 2022 2021 2020

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (2 results) (of which Invited: 1 results)

  • [Journal Article] Fabrication of hollow structures in photodegradable hydrogels using a multi-photon excitation process for blood vessel tissue engineering2020

    • Author(s)
      U. Watanabe, S. Sugiura, M. Kakehata, F. Yanagawa, T. Takagi, K. Sumaru, T. Satoh, M. Tamura, Y. Hosokawa and K. Torizuka
    • Journal Title

      Micromachines

      Volume: 11 Issue: 7 Pages: 679-679

    • DOI

      10.3390/mi11070679

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Stepwise construction of dynamic microscale concentration gradients around hydrogel-encapsulated cells in a microfluidic perfusion culture device2020

    • Author(s)
      S. Yamahira, T. Satoh, F. Yanagawa, M. Tamura, T. Takagi, E. Nakatani, Y. Kusama, K. Sumaru, S. Sugiura and T. Kanamori
    • Journal Title

      Royal Society Open Science

      Volume: 7 Issue: 7 Pages: 200027-200027

    • DOI

      10.1098/rsos.200027

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 圧力駆動型生体模倣システムによる微小血管組織の構築2022

    • Author(s)
      杉浦 慎治、進 和美
    • Organizer
      化学とマイクロ・ナノシステム学会 第46回研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 圧力駆動型生体模倣システム(PD-MPS)の開発2021

    • Author(s)
      杉浦 慎治
    • Organizer
      第17回 PhotoLIFEワークショップ
    • Related Report
      2021 Annual Research Report
    • Invited

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Published: 2019-04-18   Modified: 2024-01-30  

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