| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2022: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Outline of Final Research Achievements |
Uniform droplets using microfluidic devices have become widely used in industry, but giant unilamellar vesicles (GUVs) made of lipid bilayers have not yet reached that stage. The main reason is the difficulty in producing uniform GUVs. In this work, we established a simple and highly efficient method for producing uniform bioreactors composed of nano- to picoliter-sized GUVs using microfluidics. We have also shown that this system can be used as a platform for protein synthesis reactions and membrane protein reconstitution, as well as for membrane fusion and nucleic acid amplification reactions for the detection of disease biomarkers.
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