• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of a non-invasive cellular thermometer using Raman spectroscopy

Research Project

Project/Area Number 19H02744
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 34020:Analytical chemistry-related
Research InstitutionAoyama Gakuin University (2020-2022)
The University of Tokyo (2019)

Principal Investigator

Shimada Rintaro  青山学院大学, 理工学部, 助教 (70548940)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2021: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2020: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2019: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Keywordsラマン分光 / 温度計測 / 多変量解析 / 分光画像計測
Outline of Research at the Start

本研究は、生体分子そのものとレーザー光の相互作用により発生するラマン散乱光をもとに、プローブ分子などを用いず非侵襲的に細胞内物質の温度を直接計測する新たな細胞内部温度可視化手法の開発を行う。自家蛍光などの背景信号の強い生体試料においても正確かつ高速に温度分布計測が可能な測定法を確立し、もって多様な分子種が共存する複雑な化学環境である細胞内の化学反応場の理解に資することを目的とする。

Outline of Final Research Achievements

The present research was conducted to develop a new microscope that uses Raman spectroscopy to visualize the distribution of substances and temperature inside cells without staining or tagging. We developed a novel microscopic imaging system capable of high-speed observation of Raman scattering over a wide (-2000 to +2000 cm-1) spectral range. Using the low-frequency Raman signal, we visualized the distribution of proteins, lipids, and water inside living cells. Furthermore, we developed a new noise reduction technique for signal analysis. Using the forged instrument, we performed temperature distribution imaging of living cells and obtained results suggesting the existence of temperature inhomogeneity inside the cells.

Academic Significance and Societal Importance of the Research Achievements

本研究ではラマン分光を基盤技術として用い、細胞内部の物質分布や温度分布を非染色で可視化する新しい分光顕微鏡の開発を目的として研究を行った。本研究で開発した装置は細胞内化学環境を侵襲することなく温度計測を行う新たな方法論を提供するものであり、細胞機能や細胞活性の評価に利用可能であると期待される。

Report

(4 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (16 results)

All 2023 2022 2021 2020 2019

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (12 results) (of which Int'l Joint Research: 2 results,  Invited: 4 results)

  • [Journal Article] Controlling Diradical Character of Photogenerated Colored Isomers of Phenoxyl-Imidazolyl Radical Complexes2022

    • Author(s)
      Nishijima Moe、Mutoh Katsuya、Shimada Rintaro、Sakamoto Akira、Abe Jiro
    • Journal Title

      Journal of the American Chemical Society

      Volume: 144 Issue: 37 Pages: 17186-17197

    • DOI

      10.1021/jacs.2c07562

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of intracellular lipid metabolism in brown adipocytes by time-lapse ultra-multiplex CARS microspectroscopy with an onstage incubator2021

    • Author(s)
      Takei Yuki、Hirai Rie、Fukuda Aya、Miyazaki Shinichi、Shimada Rintaro、Okamatsu-Ogura Yuko、Saito Masayuki、Leproux Philippe、Hisatake Koji、Kano Hideaki
    • Journal Title

      The Journal of Chemical Physics

      Volume: 155 Issue: 12 Pages: 125102-125102

    • DOI

      10.1063/5.0063250

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Parallelized shifted‐excitation Raman difference spectroscopy for fluorescence rejection in a temporary varying system2019

    • Author(s)
      Shimada Rintaro、Nakamura Takashi、Ozawa Takeaki
    • Journal Title

      Journal of Biophotonics

      Volume: 12 Issue: 12

    • DOI

      10.1002/jbio.201960028

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comprehensive modeling of bloodstain aging by multivariate Raman spectral resolution with kinetics2019

    • Author(s)
      Takamura Ayari、Watanabe Daisuke、Shimada Rintaro、Ozawa Takeaki
    • Journal Title

      Communications Chemistry

      Volume: 2 Issue: 1 Pages: 115-115

    • DOI

      10.1038/s42004-019-0217-1

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Mining Raman Big Data with Independent Component Analysis Based Blind Source Separation Algorithm2023

    • Author(s)
      Rintaro Shimada
    • Organizer
      日本化学会第103春季年会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] メカニカルストリークカメラを用いた顕微ラマン分光計による高速低波数ラマンイメージング2022

    • Author(s)
      島田 林太郎、坂本 章
    • Organizer
      分子科学討論会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 顕微ラマンイメージングを用いたメダカのウロコの構造解析2022

    • Author(s)
      中川 瑳子, 島田 林太郎, 奈良 雅之, 坂本 章
    • Organizer
      日本分光学会年次講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ラマンビッグデータの時代にむけて2022

    • Author(s)
      島田 林太郎
    • Organizer
      日本分光学会年次講演会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 顕微ラマン分光イメージングのマルチプレックス化とその生細胞への応用2022

    • Author(s)
      柳沼 達也, 島田 林太郎, 坂本 章
    • Organizer
      分子科学討論会
    • Related Report
      2021 Annual Research Report
  • [Presentation] メカニカルストリークカメラを用いた 高速広帯域顕微ラマンイメージング2021

    • Author(s)
      島田 林太郎
    • Organizer
      日本分光学会生細胞分光部会シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] 独立成分分析による多変量ラマン信号成分分解の検討2021

    • Author(s)
      島田 林太郎
    • Organizer
      日本化学会第102春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Mining Large Raman Spectroscopic Data Beyond the Shot Noise Limit2020

    • Author(s)
      Rintaro Shimada, Takeaki Ozawa
    • Organizer
      Asian Spectroscopy Conference 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] メカニカルストリークカメラを用いた顕微ラマン分光計2020

    • Author(s)
      島田林太郎、小澤岳昌
    • Organizer
      日本光学会年次学術講演会 Optics & Photonics Japan
    • Related Report
      2020 Annual Research Report
  • [Presentation] 大規模ラマンハイパースペクトルデータの生物応用にむけて2019

    • Author(s)
      島田林太郎,小澤岳昌
    • Organizer
      分光学会生細胞分光部会シンポジウム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Raman Microscopy with a Mechanical Streak Camera2019

    • Author(s)
      Shimada, Rintaro; Chiu, Liang-da; Ozawa, Takeaki,
    • Organizer
      International Conference on Advanced Vibrational Spectroscopy
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 分散安定化によるラマンハイパースペクトルデータの行列分解成分数の改善2019

    • Author(s)
      島田林太郎,小澤岳昌
    • Organizer
      日本分析化学会第68年会
    • Related Report
      2019 Annual Research Report

URL: 

Published: 2019-04-18   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi