Elucidation of Molecular Mechanism for Activation of High-Valent Metal-Oxo species through their interaction with G-Quadruplexes and Creation of Novel DNAzymes

• Project/Area Number: 19H02824
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 37010:Bio-related chemistry
• Research Institution: University of Tsukuba
• Principal Investigator: Yamamoto Yasuhiko 筑波大学, 数理物質系, 教授 (00191453)
• Co-Investigator(Kenkyū-buntansha): 鈴木 秋弘 長岡工業高等専門学校, 物質工学科, 教授 (60179190) ; 百武 篤也 筑波大学, 数理物質系, 准教授 (70375369)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2021: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2019: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: 四重鎖DNA / Gカルテット / ヘム / 酸化触媒作用 / 機能性核酸 / G-カルテット / 分子認識 / 触媒機構 / テロメア / 軸配位子 / 高原子価金属オキソ種

Outline of Research at the Start

四重鎖DNAまたは四重鎖RNAとヘムの複合体が示す酸化触媒作用の分子機構を、反応中間体として生成するオキソ鉄４価ポルフィリンπカチオンラジカル錯体の反応性の制御機構の解明を通して明らかにする研究である。そして、高温および有機溶媒との混合水溶液中でも利用できると共に、簡便に大量合成可能な画期的酵素を創製するために有用な知見を得る。本研究を通して、ヘムをもつ核酸酵素の酸化触媒活性の発現で鍵となる反応中間体である高酸化状態の鉄ポルフィリン錯体が生成する分子機構が解明されることになり、生物無機化学における革新的ブレークスルーにつながる研究成果が得られる。

Outline of Final Research Achievements

Heme(Fe3+) binds to non-canonical G-quadruplex DNAs to form complexes (heme-DNAzymes) that exhibit peroxidase and peroxygenase activities. We found that the catalytic cycle of the heme-DNAzyme involves an iron(IV)oxo porphyrin π-cation radical intermediate known as Compound I formed through heterolytic O-O bond cleavage of an Fe-bound hydroperoxo ligand (Fe-OOH) in Compound 0. We also found that heme(Fe3+) binds selectively to the 3’-terminal G-quartet of the DNA, and a water molecule (AxH2O) sandwiched between the heme and G-quartet planes is coordinated to the heme Fe atom as an axial ligand. The polarization of AxH2O is thought to be considerably greater than that of an ordinary H2O molecule. Such unique electronic nature of AxH2O was found to be responsible for the catalytic activity of the heme-DNAzyme. We also found that the peroxidase activity of the heme-DNAzyme can be controlled through the local heme environment.

Academic Significance and Societal Importance of the Research Achievements

本研究により、ヘムDNA酵素におけるヘムの機能の調節機構が解明された。ヘムDNA酵素の触媒サイクルでは、酵素ペルオキシダーゼとは異なる分子機構で、オキソ鉄４価ポルフィリンπカチオンラジカル錯体（Compound I）が反応中間体として生成することが明らかになった。ヘムDNA酵素のヘム鉄の軸配位子である水分子が、Compound Iの生成に重要な役割を担っていること、ヘムDNA酵素が示すヘムタンパク質類似の様々な分光学的性質をもたらしていることを発見した研究成果は、水分子の新しい機能および疎水性空間で孤立する水分子のユニークな物理化学的性質が解明されたという観点から、学術的な意義は大きい。

Research Products

• [Journal Article] Nature of a H2O Molecule Confined in the Hydrophobic Interface between the Heme and G-Quartet Planes in a Heme-DNA Complex (2022)
  • Author(s): Nakajima Yusuke、Momotake Atsuya、Suzuki Akihiro、Neya Saburo、Yamamoto Yasuhiko
  • Journal Title: Biochemistry Volume: － Issue: 7 Pages: 523-534
  • DOI: 10.1021/acs.biochem.1c00751
  • Peer Reviewed
• [Journal Article] Effects of Heme Electronic Structure and Local Heme Environment on Catalytic Activity of a Peroxidase-Mimicking Heme-DNAzyme (2021)
  • Author(s): Hagiwara Shota、Momotake Atsuya、Ogura Takashi、Yanagisawa Sachiko、Suzuki Akihiro、Neya Saburo、Yamamoto Yasuhiko
  • Journal Title: Inorganic Chemistry Volume: 60 Issue: 15 Pages: 11206-11213
  • DOI: 10.1021/acs.inorgchem.1c01179
  • Peer Reviewed
• [Journal Article] Molecular recognition of G-quadruplex DNA by Pheophorbide a (2021)
  • Author(s): Okamoto China、Momotake Atsuya、Kobayashi Masami、Yamamoto Yasuhiko
  • Journal Title: Chemistry Letters Volume: - Issue: 6 Pages: 1278-1281
  • DOI: 10.1246/cl.210130
  • NAID: 130008053396
  • Peer Reviewed
• [Journal Article] A cationic copolymer as a cocatalyst for a peroxidase-mimicking heme-DNAzyme (2021)
  • Author(s): Araki Haruka、Hagiwara Shota、Shinomiya Ryosuke、Momotake Atsuya、Kotani Hiroaki、Kojima Takahiko、Ochiai Takuro、Shimada Naohiko、Maruyama Atsushi、Yamamoto Yasuhiko
  • Journal Title: Biomaterials Science Volume: 9 Issue: 18 Pages: 6142-6152
  • DOI: 10.1039/d1bm00949d
  • Peer Reviewed
• [Journal Article] Structural and functional characterization of complexes between heme and dimeric parallel G-quadruplex DNAs (2021)
  • Author(s): Okamoto China、Momotake Atsuya、Yamamoto Yasuhiko
  • Journal Title: Journal of Inorganic Biochemistry Volume: 216 Pages: 111336-111336
  • DOI: 10.1016/j.jinorgbio.2020.111336
  • Peer Reviewed
• [Journal Article] Stepwise binding of a cationic phthalocyanine derivative to an all parallel-stranded tetrameric G-quadruplex DNA (2020)
  • Author(s): Uchiyama Mami、Okamoto China、Momotake Atsuya、Ikeue Takahisa、Yamamoto Yasuhiko
  • Journal Title: Journal of Inorganic Biochemistry Volume: 213 Pages: 111270-111270
  • DOI: 10.1016/j.jinorgbio.2020.111270
  • Peer Reviewed
• [Journal Article] Effect of the Electron Density of the Heme Fe Atom on the Nature of Fe-O2 Bonding in Oxy Myoglobin (2020)
  • Author(s): Yamamoto Yasuhiko、Hasegawa Kazuyasu、Shibata Tomokazu、Momotake Atsuya、Ogura Takashi、Yanagisawa Sachiko、Neya Saburo、Suzuki Akihiro、Kobayashi Yasuhiro、Saito Makina、Seto Makoto、Ohta Takehiro
  • Journal Title: Inorganic Chemistry Volume: 60 Issue: 2 Pages: 1021-1027
  • DOI: 10.1021/acs.inorgchem.0c03123
  • Peer Reviewed
• [Journal Article] Photogeneration of Reactive Oxygen Species from Water-Soluble Phthalocyanine Derivatives Bound to a G-Quadruplex DNA (2020)
  • Author(s): Uchiyama Mami、Momotake Atsuya、Ikeue Takahisa、Yamamoto Yasuhiko
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 93 Issue: 12 Pages: 1504-1508
  • DOI: 10.1246/bcsj.20200178
  • NAID: 130007955892
  • Peer Reviewed
• [Journal Article] Characterization of Structure and Catalytic Activity of a Complex between Heme and an All Parallel-Stranded Tetrameric G-Quadruplex Formed from DNA/RNA Chimera Sequence d(TTA)r(GGG)dT (2020)
  • Author(s): Hayasaka Kosuke、Shibata Tomokazu、Sugahara Aya、Momotake Atsuya、Matsui Toru、Neya Saburo、Ishizuka Takumi、Xu Yan、Yamamoto Yasuhiko
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 93 Issue: 5 Pages: 621-629
  • DOI: 10.1246/bcsj.20200013
  • NAID: 130007842975
  • Peer Reviewed
• [Journal Article] Specific Binding of an Anionic Phthalocyanine Derivative to G-Quadruplex DNAs (2020)
  • Author(s): Uchiyama Mami、Momotake Atsuya、Kobayashi Nagao、Yamamoto Yasuhiko
  • Journal Title: Chemistry Letters Volume: 49 Issue: 5 Pages: 530-533
  • DOI: 10.1246/cl.200110
  • NAID: 130007840180
  • Peer Reviewed
• [Journal Article] Multimodal Multiphoton Imaging of the Lipid Bilayer by Dye-Based Sum-Frequency Generation and Coherent Anti-Stokes Raman Scattering (2020)
  • Author(s): Mizuguchi Takaha、Momotake Atsuya、Hishida Mafumi、Yasui Masato、Yamamoto Yasuhiko、Saiki Toshiharu、Nuriya Mutsuo
  • Journal Title: Analytical Chemistry Volume: 92 Issue: 8 Pages: 5656-5660
  • DOI: 10.1021/acs.analchem.0c00673
  • Peer Reviewed
• [Journal Article] Monitoring the morphological evolution of giant vesicles by azo dye-based sum-frequency generation (SFG) microscopy (2020)
  • Author(s): Momotake Atsuya、Mizuguchi Takaha、Hishida Mafumi、Yamamoto Yasuhiko、Yasui Masato、Nuriya Mutsuo
  • Journal Title: Colloids and Surfaces B: Biointerfaces Volume: 186 Pages: 110716-110716
  • DOI: 10.1016/j.colsurfb.2019.110716
  • Peer Reviewed / Open Access
• [Journal Article] Identification of Intermediates in Peroxidase Catalytic Cycle of a DNAzyme Possessing Heme (2019)
  • Author(s): Shinomiya Ryosuke、Araki Haruka、Momotake Atsuya、Kotani Hiroaki、Kojima Takahiko、Yamamoto Yasuhiko
  • Journal Title: Bulletin of the Chemical Society of Japan Volume: 92 Issue: 10 Pages: 1729-1736
  • DOI: 10.1246/bcsj.20190157
  • NAID: 130007728639
  • Peer Reviewed
• [Presentation] 四重鎖DNAのキラターゼ活性 (2022)
  • Author(s): 鳥越果林，岡本千奈，百武篤也，山本泰彦
  • Organizer: 日本化学会第102春季年会(2022)
• [Presentation] フェオホルバイドaとグアニン四重鎖DNAの相互作用 (2022)
  • Author(s): 岡本千奈，百武篤也，小林正美，山本泰彦
  • Organizer: 日本化学会第102春季年会(2022)
• [Presentation] ヘム-DNA酵素におけるヘムとG-カルテットの接触界面に存在する水分子の解析 (2022)
  • Author(s): 中島優輔，百武篤也，鈴木秋弘，根矢三郎，山本泰彦
  • Organizer: 日本化学会第102春季年会(2022)
• [Presentation] アニオン性フタロシアニン-四重鎖DNA複合体の解析 (2022)
  • Author(s): 小田原佑典，百武篤也，山本泰彦
  • Organizer: 日本化学会第102春季年会(2022)
• [Presentation] 熱安定性が高いヘム-DNA酵素のペルオキシダーゼ活性の解析 (2022)
  • Author(s): 深澤大輝，百武篤也，山本泰彦
  • Organizer: 日本化学会第102春季年会(2022)
• [Presentation] Characterization of a Complex Between Heme(Fe3+) and a G-quadruplex DNA (2021)
  • Author(s): Y. Nakajima, S. Neya, A. Suzuki, A. Momotake, and Y. Yamamoto
  • Organizer: Joint Conference of 22nd ISMAR, 9th APNMR, 60th NMRSJ(2021), and 60th SEST
  • Int'l Joint Research
• [Presentation] Molecular Recognition of G-Quadruplex DNAs by Tetrapyrrole Macrocyclic Ligands (2021)
  • Author(s): C. Okamoto, A. Momotake, and Y. Yamamoto
  • Organizer: Joint Conference of 22nd ISMAR, 9th APNMR, 60th NMRSJ(2021), and 60th SEST
  • Int'l Joint Research
• [Presentation] Characterization of Myoglobins Where Exogenous Ligands Replace the Proximal Histidine (2021)
  • Author(s): R. Murata, T. Sugita, T. Matsuo, S. Hirota, S. Yanagisawa, S. Neya, A. Suzuki, O. Shoji, Y. Watanabe, A. Momotake, and Y. Yamamoto
  • Organizer: Joint Conference of 22nd ISMAR, 9th APNMR, 60th NMRSJ(2021), and 60th SEST
  • Int'l Joint Research
• [Presentation] Heme Electronic Structure and Local Heme Environment of a Peroxidase-Mimicking Heme-DNAzyme (2021)
  • Author(s): S. Hagiwara, A. Sugahara, R. Shinomiya, S. Neya, A. Suzuki, A. Momotake, and Y. Yamamoto
  • Organizer: Joint Conference of 22nd ISMAR, 9th APNMR, 60th NMRSJ(2021), and 60th SEST
  • Int'l Joint Research
• [Presentation] Interaction between Heme and Non-Standard Base Inosine(I)-Quartet (2021)
  • Author(s): A. Momotake, A. Sugahara, K. Torigoe, Y. Nakajima, S. Neya, A. Suzuki, and Y. Yamamoto
  • Organizer: Joint Conference of 22nd ISMAR, 9th APNMR, 60th NMRSJ(2021), and 60th SEST
  • Int'l Joint Research
• [Presentation] Interaction between Zn(II) Phthalocyanine Derivatives and a G-Quadruplex DNA (2021)
  • Author(s): S. Homma, M. Uchiyama, A. Momotake, T. Ikeue, and Y. Yamamoto
  • Organizer: Joint Conference of 22nd ISMAR, 9th APNMR, 60th NMRSJ(2021), and 60th SEST
  • Int'l Joint Research
• [Presentation] Elucidation of the Regulatory Mechanism for Peroxidase Activity of DNAzyme Possessing Heme (2021)
  • Author(s): S. Hagiwara, R. Shinomiya, A. Momotake, S. Neya, A. Suzuki, and Y. Yamamoto
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2021
  • Int'l Joint Research
• [Presentation] Characterization of a Complex Consisting of Paramagnetic Heme(Fe3+) and G-Guadruplex DNA Formed from d(TAGGGTGGGTTGGGTGIG) (2021)
  • Author(s): Y. Nakajima, S. Neya, A. Suzuki, A. Momotake, and Y. Yamamoto
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2021
  • Int'l Joint Research
• [Presentation] Study of Myoglobin Reconstituted with a Chemically-Modified Heme Cofactor Possessing a Trifluoro Methyl Group at 5-Meso Position (2021)
  • Author(s): R. Murata, A. Momotake, A. Suzuki, T. Matsuo, S. Hirota, S. Neya, M. Senda, T. Senda, and Y. Yamamoto
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2021
  • Int'l Joint Research
• [Presentation] Interaction between Heme and I-quartet in a Heme-DNAzyme (2021)
  • Author(s): T. Torigoe, A. Momotake, S. Neya, S. Yanagisawa, T. Ogura, A. Suzuki, and Y. Yamamoto
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2021
  • Int'l Joint Research
• [Presentation] Study on the Effect of G-Quadruplex DNA on Photochemical Behaviors of Cationic Phthalocyanine Derivatives (2021)
  • Author(s): M. Uchiyama, A. Momotake, T. Ikeue, and Y. Yamamoto
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2021
  • Int'l Joint Research
• [Presentation] Molecular Recognition of G-Quadruplex DNA by Pheophorbide a (2021)
  • Author(s): C. Okamoto, A. Momotake, M. Kobayashi, and Y. Yamamoto
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2021
  • Int'l Joint Research
• [Presentation] Interaction of Cationic Phthalocyanine Derivatives with Guanine Nucleobases (2021)
  • Author(s): S. Homma, M. Uchiyama, A. Momotake, T. Ikeue, and Y. Yamamoto
  • Organizer: The International Chemical Congress of Pacific Basin Societies 2021
  • Int'l Joint Research
• [Presentation] 生命金属錯体と核酸の組織化による機能創発と生命金属元素戦略解明への応用 (2021)
  • Author(s): 山本泰彦
  • Organizer: 新学術領域研究「生命金属科学」領域会議 第四回地方巡業（東京）
• [Presentation] Interaction between Tetrapyrrole Macrocycles and Quadruplex Nucleic Acids (2021)
  • Author(s): Y. Yamamoto
  • Organizer: 60th Annual Meeting of the Nuclear Magnetic Resonance Society of Japan (2021)
• [Presentation] 生命金属錯体と核酸の組織化による機能創発と生命金属元素戦略解明への応用 (2021)
  • Author(s): 山本泰彦
  • Organizer: 生命金属科学 ルスツ夏合宿
• [Presentation] 四重鎖 DNA とフタロシアニン誘導体の複合体の解析 (2020)
  • Author(s): 内山真見、岡本千奈、百武篤也、山本泰彦、池上崇久
  • Organizer: 第59回NMR討論会(2020)
• [Presentation] メソ位にトリフルオロメチル基を導入したヘムをもつミオグロビンの研究 (2020)
  • Author(s): 村田莉恵、百武篤也、山本泰彦、鈴木秋弘、松尾貴史、廣田俊、根矢三郎
  • Organizer: 日本化学会第100春季年会 (2020)
• [Presentation] 四重鎖DNAとフタロシアニン誘導体の複合体 (2020)
  • Author(s): 内山真見、柴田友和、百武篤也、池上崇久、松井亨、山本泰彦
  • Organizer: 日本化学会第100春季年会 (2020)
• [Presentation] ヘム－DNA 複合体のイミダゾール付加物の解析 (2020)
  • Author(s): 中島優輔、百武篤也、山本泰彦
  • Organizer: 日本化学会第100春季年会 (2020)
• [Presentation] カチオン性共重合体によるヘム-DNA 複合体のペルオキシダーゼ活性の増大 (2020)
  • Author(s): 荒木はるか、百武篤也、山本泰彦、落合拓郎、嶋田直彦、丸山厚
  • Organizer: 日本化学会第100春季年会 (2020)
• [Presentation] ヒトテロメア関連塩基配列のDNA が2 分子で形成する平行型四重鎖DNA およびそのヘムとの複合体の研究 (2020)
  • Author(s): 岡本千奈、篠宮僚介、山田果央、百武篤也、山本泰彦
  • Organizer: 日本化学会第100春季年会 (2020)
• [Presentation] ヘム-DNA 複合体のペルオキシダーゼ活性におけるPush 効果とPull 効果の相関関係の解析 (2020)
  • Author(s): 萩原翔太、篠宮僚介、百武篤也、鈴木秋弘、根矢三郎、山本泰彦
  • Organizer: 日本化学会第100春季年会 (2020)
• [Presentation] Deoxyribozyme Possessing Heme (2019)
  • Author(s): Y. Yamamoto, H. Araki, K. Hayasaka, S. Nakamura,T. Shibata, A. Momotake, and H. Hemmi
  • Organizer: 61st Experimental Nuclear Magnetic Resonance Conference
  • Int'l Joint Research
• [Presentation] Structures and Catalytic Activities of DNA Complexes Consisting of Chemically Modified Heme and Tetrameric G-Quadruplex DNA (2019)
  • Author(s): R. Shinomiya, A. Momotake, Y. Katahira, H. Araki, T. Shibata, S. Yanagisawa, T. Ogura, A. Suzuki, S. Neya, and Y. Yamamoto
  • Organizer: 15th International Symposium on Applied Bioinorganic Chemistry (ISABC15)
  • Int'l Joint Research
• [Presentation] Heme-DNAzyme (2019)
  • Author(s): Y. Yamamoto, R. Shinomiya, H. Araki, T. Shibata, A. Momotake, S. Yanagisawa, T. Ogura, A. Suzuki, S. Neya, and H. Hemmi
  • Organizer: Commemorative International Symposium of the Japan Society of Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Heme Meets DNA. (2019)
  • Author(s): Y. Yamamoto, R. Shinomiya, H. Kotani, H. Araki, A. Momotake, T. Shibata, S. Yanagisawa, T. Ogura, H. Hemmi, A. Suzuki, S. Neya, and T. Kojima
  • Organizer: 19th International Conference on Biological Inorganic Chemistry (ICBIC-19)
  • Int'l Joint Research / Invited
• [Presentation] DNAzyme Possessing Heme (2019)
  • Author(s): Y. Yamamoto, R. Shinomiya, H. Araki, T. Shibata, A. Momotake, S. Yanagisawa, T. Ogura, A. Suzuki, and S. Neya
  • Organizer: The 46th International Symposium on Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Enhancement of Peroxidase Activity of Heme-DNAzyme through Interaction with Cationic Copolymers (2019)
  • Author(s): H. Araki, A. Momotake, Y. Yamamoto, T. Ochiai, N. Shimada, and A. Maruyama
  • Organizer: The 46th International Symposium on Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Complexes between G-quadruplex DNA and Phthalocyanines (2019)
  • Author(s): M. Uchiyama, T. Shibata, A. Momotake, T. Ikeue, and Y. Yamamoto
  • Organizer: The 46th International Symposium on Nucleic Acids Chemistry
  • Int'l Joint Research
• [Presentation] Electronic Control of Myoglobin Function (2019)
  • Author(s): T. Sugita, T. Matsuo, S. Hirota, S. Yanagisawa, T. Ogura, O. Shoji, Y. Watanabe, A. Suzuki, S. Neya, A. Momotake, and Y. Yamamoto
  • Organizer: 17th International Symposium on Blood Substitutes & Oxygen Therapeutics (XVII-ISBS-2019)
  • Int'l Joint Research
• [Presentation] ヘムをもつ DNA 酵素 (2019)
  • Author(s): 山本泰彦、 篠宮僚介、小谷弘明、 荒木はるか、柴田友和、 百武篤也、 柳澤幸子、小倉尚志、逸見光、鈴木秋弘、根矢三郎、小島隆彦
  • Organizer: 第46回生体分子科学討論会
• [Presentation] 非線形光学イメージングに向けた無蛍光性分子プローブの開発と応用 (2019)
  • Author(s): 百武篤也、水口高翔、塗谷睦生、安井正人、山本泰彦
  • Organizer: 第46回生体分子科学討論会
• [Presentation] 四重鎖 DNA の形成様式がヘムをもつ DNA 酵素の性質に与える影響 (2019)
  • Author(s): 荒川和基、山田果央、片平裕弥、越智健太郎、中山優作、荒木はるか、篠宮僚介、百武篤也、山本泰彦
  • Organizer: 第46回生体分子科学討論会
• [Presentation] 3'末端に I-カルテットをもつ四重鎖 DNA とヘムの複合体の研究 (2019)
  • Author(s): 菅原綾、篠宮僚介、木下真志、柴田友和、百武篤也、山本泰彦、 柳澤幸子、小倉尚志、鈴木秋弘、根矢三郎
  • Organizer: 第46回生体分子科学討論会
• [Presentation] ヘム鉄の軸配位子としてイミダゾールをもつミオグロビンの研究 (2019)
  • Author(s): 杉田達郎、百武篤也、山本泰彦、松尾貴史、廣田俊、鈴木秋弘、根矢三郎、荘司長三、渡辺芳人
  • Organizer: 第46回生体分子科学討論会
• [Presentation] ヘムをもつDNA 酵素の酸化触媒機構 (2019)
  • Author(s): 山本泰彦、篠宮僚介、小谷弘明、荒木はるか、柴田友和、百武篤也、柳澤幸子、小倉尚志、鈴木秋弘、根矢三郎、小島隆彦
  • Organizer: 第46回生体分子科学討論会
• [Presentation] Structural characterization of deoxyribozyme possessing heme (2019)
  • Author(s): Y. Yamamoto, Y. Suzuki, Y. Nakajima, T. Shibata, A. Momotake, S. Neya, and A. Suzuki
  • Organizer: 第58回NMR討論会(2019)
• [Presentation] 四重鎖DNAとヘムの複合体の酸化触媒機構 (2019)
  • Author(s): 菅原綾、篠宮僚介、小谷弘明、荒木はるか、柴田友和、百武篤也、柳澤幸子、小倉尚志、鈴木秋弘、根矢三郎、小島隆彦、山本泰彦
  • Organizer: 第52回酸化反応討論会
• [Book] 核酸科学ハンドブック（核酸二重鎖結合分子） (2020)
  • Author(s): 山本泰彦、百武篤也、日本核酸化学会、杉本 直己 編
  • Total Pages: 576
  • Publisher: 講談社
  • ISBN: 9784065207864
• [Remarks] 筑波大学 数理物質系 生物無機化学研究室
  • URL: https://www.chem.tsukuba.ac.jp/yamamoto/gyouseki.php
• [Remarks] 筑波大学 数理物質系 生物無機化学研究室
  • URL: http://www.chem.tsukuba.ac.jp/yamamoto/