| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2021: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Outline of Final Research Achievements |
To develop artificial RNA-binding proteins, we focused on the R1'/ R8'domains at the N- and C-termini of the human Pumilio protein (hPUM), whose involvement in RNA recognition was unclear, and clarified their functions related to RNA binding. By preparing various variants of the domain and comparing their RNA binding characteristics with the wild type, we found that these domains, which do not directly interact with the base of the target RNA, makes an important contribution to RNA recognition of hPUM by stabilizing the entire protein structure.
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