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Delivery of cargo in bacterial membrane vesicles

Research Project

Project/Area Number 19H02866
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionUniversity of Tsukuba

Principal Investigator

Toyofuku Masanori  筑波大学, 生命環境系, 准教授 (30644827)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2022: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywordsメンブレンベシクル / 微生物間相互作用 / 細菌間コミュニケーション / ファージ / シグナル伝達 / 細胞外膜小胞 / 細菌間相互作用 / 膜小胞 / 細胞間相互作用 / 微生物間コミュニケーション
Outline of Research at the Start

細菌は多様な機能を持つ細胞外膜小胞(メンブレンベシクル(MV))を放出し、生物学的に重要な役割を果たすと共にワクチン開発にも応用されている。MVは細胞間での物質運搬にも関わっており、その積荷が細胞に渡されていることは、数多くの研究例で実証されている。しかしながら、その詳細な過程やメカニズムについては全く理解されていない。本研究は、MVによる物質受け渡しプロセスとそのメカニズムの解明を目指す。

Outline of Final Research Achievements

Most bacteria release extracellular membrane vesicles (membrane vesicles (MVs)) that have diverse functions. However, the details of the MV cargo uptake in bacteria have not been fully understood. In this project, we aimed to elucidate the mechanism of MV delivery. Given that the MV formation routes impact the MV cargos, we also analyzed the MV formation mechnisms along with the function of the MVs. As a result, we were able gain insights in to how MVs are formed and the cargos are delivery to the targetting cells.

Academic Significance and Societal Importance of the Research Achievements

MVはDNA、RNA、タンパク質、シグナル化合物などを運搬することで、遺伝子の水平伝播や細菌間コミュニケーション、抗生物質耐性等、多くの機能を併せ持ち、細菌の進化や生存戦略の根幹にも関わる。さらには、MVは生物材料としても非常に注目され、創薬などで新たな分野を切り拓いている。本課題の成果によって、MVに関する基礎的な知見が得られて、それをターゲットにした、微生物利用、制御分野に新たな切り口を提供することが期待される。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (43 results)

All 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (5 results) Journal Article (15 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 15 results,  Open Access: 11 results) Presentation (17 results) (of which Int'l Joint Research: 4 results,  Invited: 14 results) Book (1 results) Remarks (4 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] チューリッヒ大学(スイス)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] 高麗大学(韓国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] チューリッヒ大学(スイス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Nanyang Technological University(シンガポール)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] チューリッヒ大学(スイス)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Composition and functions of bacterial membrane vesicles2023

    • Author(s)
      Toyofuku Masanori、Schild Stefan、Kaparakis-Liaskos Maria、Eberl Leo
    • Journal Title

      Nature Reviews Microbiology

      Volume: in press Issue: 7 Pages: 415-430

    • DOI

      10.1038/s41579-023-00875-5

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The role of peptidoglycan hydrolases in the formation and toxicity of Pseudomonas aeruginosa membrane vesicles2022

    • Author(s)
      Chen Yi-Chi、Kalawong Ratchara、Toyofuku Masanori、Eberl Leo
    • Journal Title

      microLife

      Volume: 3 Pages: 1-6

    • DOI

      10.1093/femsml/uqac009

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Physical Properties and Shifting of the Extracellular Membrane Vesicles Attached to Living Bacterial Cell Surfaces2022

    • Author(s)
      Kikuchi Yousuke、Toyofuku Masanori、Ichinaka Yuki、Kiyokawa Tatsunori、Obana Nozomu、Nomura Nobuhiko、Taoka Azuma
    • Journal Title

      Microbiology Spectrum

      Volume: 10 Issue: 6 Pages: 02165-22

    • DOI

      10.1128/spectrum.02165-22

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Single Shot of Vesicles2022

    • Author(s)
      Toyofuku Masanori、Kikuchi Yousuke、Taoka Azuma
    • Journal Title

      Microbes and Environments

      Volume: 37 Issue: 6 Pages: n/a

    • DOI

      10.1264/jsme2.ME22083

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Controllable secretion of multilayer vesicles driven by microbial polymer accumulation2022

    • Author(s)
      Koh Sangho、Sato Michio、Yamashina Kota、Usukura Yuki、Toyofuku Masanori、Nomura Nobuhiko、Taguchi Seiichi
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 1-9

    • DOI

      10.1038/s41598-022-07218-z

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phage Genes Induce Quorum Sensing Signal Release through Membrane Vesicle Formation2022

    • Author(s)
      Yasuda Marina、Yamamoto Tatsuya、Nagakubo Toshiki、Morinaga Kana、Obana Nozomu、Nomura Nobuhiko、Toyofuku Masanori
    • Journal Title

      Microbes and Environments

      Volume: 37 Issue: 1 Pages: n/a

    • DOI

      10.1264/jsme2.ME21067

    • NAID

      130008144944

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Autolysis‐mediated membrane vesicle formation in Bacillus subtilis2021

    • Author(s)
      Abe Kimihiro、Toyofuku Masanori、Nomura Nobuhiko、Obana Nozomu
    • Journal Title

      Environmental Microbiology

      Volume: - Issue: 5 Pages: 2632-2647

    • DOI

      10.1111/1462-2920.15502

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biogenesis of Outer Membrane Vesicles Concentrates the Unsaturated Fatty Acid of Phosphatidylinositol in Capnocytophaga ochracea2021

    • Author(s)
      Naradasu Divya、Miran Waheed、Sharma Shruti、Takenawa Satoshi、Soma Takamitsu、Nomura Nobuhiko、Toyofuku Masanori、Okamoto Akihiro
    • Journal Title

      Frontiers in Microbiology

      Volume: 12 Pages: 682685-682685

    • DOI

      10.3389/fmicb.2021.682685

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Mycolic acid-containing bacteria trigger distinct types of membrane vesicles through different routes2021

    • Author(s)
      Nagakubo Toshiki、Tahara Yuhei O.、Miyata Makoto、Nomura Nobuhiko、Toyofuku Masanori
    • Journal Title

      iScience

      Volume: 24 Issue: 1 Pages: 102015-102015

    • DOI

      10.1016/j.isci.2020.102015

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Diversity of physical properties of bacterial extracellular membrane vesicles revealed through atomic force microscopy phase imaging2020

    • Author(s)
      Kikuchi Yousuke、Obana Nozomu、Toyofuku Masanori、Kodera Noriyuki、Soma Takamitsu、Ando Toshio、Fukumori Yoshihiro、Nomura Nobuhiko、Taoka Azuma
    • Journal Title

      Nanoscale

      Volume: 12 Issue: 14 Pages: 7950-7959

    • DOI

      10.1039/c9nr10850e

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Involvement of membrane vesicles in long‐chain‐ AHL delivery in Paracoccus species2020

    • Author(s)
      Morinaga Kana、Nagakubo Toshiki、Nomura Nobuhiko、Toyofuku Masanori
    • Journal Title

      Environmental Microbiology Reports

      Volume: 12 Issue: 3 Pages: 355-360

    • DOI

      10.1111/1758-2229.12843

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sex Steroids Induce Membrane Stress Responses and Virulence Properties in Pseudomonas aeruginosa2020

    • Author(s)
      Vidaillac Celine、......Toyofuku Masanori、.....Henriques Normark Birgitta、Chotirmall Sanjay H.
    • Journal Title

      mBio

      Volume: 11 Issue: 5

    • DOI

      10.1128/mbio.01774-20

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cracking open bacterial membrane vesicles2020

    • Author(s)
      Nagakubo T, Nomura N, Toyofuku M.
    • Journal Title

      Frontiers in Microbiology

      Volume: 10 Pages: 3026-3026

    • DOI

      10.3389/fmicb.2019.03026

    • NAID

      120007132782

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Peculiarities of biofilm formation by Paracoccus denitrificans and associated factors2020

    • Author(s)
      Morinaga K, Yoshida K, Takahashi K, Nomura N, Toyofuku M
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 104 Issue: 6 Pages: 2427-2433

    • DOI

      10.1007/s00253-020-10400-w

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bacterial communication through membrane vesicles2019

    • Author(s)
      Toyofuku M
    • Journal Title

      Biotechnol Biochem

      Volume: 83 Issue: 9 Pages: 1599-1605

    • DOI

      10.1080/09168451.2019.1608809

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] Streptococcus mutansの微生物間コミュニケーションを介した膜小胞の産生2023

    • Author(s)
      伊藤碧美、山本千佳、永沢亮、尾花望、野村暢彦、豊福雅典
    • Organizer
      日本農芸化学会2023年度大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Membrane vesicle formation is triggered by cell death via quorum sensing in Streptococcus mutans2022

    • Author(s)
      伊藤碧美、山本千佳、永沢亮、尾花望、野村暢彦、豊福雅典
    • Organizer
      International Symposium on Microbial Ecology
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Streptococcus mutansの微生物間コミュニケーションを介した膜小胞の形成2022

    • Author(s)
      伊藤碧美、山本千佳、永沢亮、尾花望、野村暢彦、豊福雅典
    • Organizer
      日本微生物生態学会第35回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Applying single cell imaging to understand bacterial membrane vesicle transport2022

    • Author(s)
      豊福雅典
    • Organizer
      日本農芸化学会2022年度大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] イメージングで捉える細菌の細胞外膜小胞輸送」2022

    • Author(s)
      豊福雅典
    • Organizer
      第95回 細菌学会総会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Bacterial Interactions Through Membrane Vesicles2021

    • Author(s)
      Masanori Toyofuku
    • Organizer
      FEMS-ASM World Microbe Forum
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Bacterial communication through MVs2021

    • Author(s)
      Masanori Toyofuku
    • Organizer
      EMBO workshop
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 膜小胞を介した細菌間コミュニケーションの研究2021

    • Author(s)
      豊福雅典
    • Organizer
      日本微生物学連盟 野本賞 受賞講演
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Bacterial Trafficking of Biomolecules Through Membrane Vesicles2021

    • Author(s)
      豊福雅典
    • Organizer
      Annual meeting of electrokinetic society Japan
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] メンブレンベシクル形成機構の多様性と普遍性2021

    • Author(s)
      豊福雅典
    • Organizer
      日本農芸化学会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 細菌における多様なMV形成機構2020

    • Author(s)
      豊福雅典
    • Organizer
      日本生化学会大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Bacterial membrane vesicle formation through cell death2020

    • Author(s)
      豊福雅典
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 微生物が放出する多様なベシクル2020

    • Author(s)
      豊福雅典
    • Organizer
      高分子と水・分離に関する研究会および2019年度界面動電現象研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] MV形成機構におけるパラダイムシフトとその応用2020

    • Author(s)
      豊福雅典
    • Organizer
      第93回日本細菌学会総会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 細菌のメンブレンベシクル形成機構と機能2019

    • Author(s)
      豊福雅典
    • Organizer
      第10回 愛媛微生物学ネットワーク
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ベシクルを介した細菌の化学コミュニケーション2019

    • Author(s)
      豊福雅典
    • Organizer
      第14回化学生態研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] How do bacteria “really” communicate2019

    • Author(s)
      Masanori Toyofuku
    • Organizer
      Marine Biotechnology Conference 2019,
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] Bacterial Membrane Vesicles2020

    • Author(s)
      Toyofuku M, Tashiro Y, Nomura N, Eberl L
    • Total Pages
      18
    • Publisher
      Springer
    • Related Report
      2019 Annual Research Report
  • [Remarks] 研究室ホームページ

    • URL

      https://sites.google.com/alumni.tsukuba.ac.jp/masatoyofuku/home

    • Related Report
      2022 Annual Research Report
  • [Remarks] 研究室HP

    • URL

      https://sites.google.com/alumni.tsukuba.ac.jp/masatoyofuku/home

    • Related Report
      2021 Annual Research Report
  • [Remarks] 研究者総覧

    • URL

      https://trios.tsukuba.ac.jp/researcher/0000003299

    • Related Report
      2020 Annual Research Report
  • [Remarks] 研究室HP

    • URL

      http://www.envr.tsukuba.ac.jp/~microbio/

    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 対象物質が封入された膜小胞の製造方法2020

    • Inventor(s)
      豊福雅典、野村暢彦、臼倉雄紀
    • Industrial Property Rights Holder
      豊福雅典、野村暢彦、臼倉雄紀
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2020 Annual Research Report

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Published: 2019-04-18   Modified: 2024-01-30  

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