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Evaluation of unique autothermal thermophilic digestion process showing no nitrification and denitrification

Research Project

Project/Area Number 19H02875
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionKyushu University

Principal Investigator

SAKAI KENJI  九州大学, 農学研究院, 教授 (50205704)

Co-Investigator(Kenkyū-buntansha) 田代 幸寛  九州大学, 農学研究院, 准教授 (90448481)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2021: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywords自家熱型高温好気消化(ATAD)法 / 複合微生物工学 / 液肥 / し尿処理 / SDGs / 総括酸素移動容量係数(KLa) / 剪断力 / 細菌群集構造変化 / 自家熱型高温好気消化 / 有機液肥製造 / 有機排水処理 / 複合微生物系 / メタゲノム解析 / 総括酸素移動容量係数 / 硝化脱窒 / 循環型社会 / 持続型生産 / ATAD / 高温好気消化 / 自吸式通気撹拌装置 / 液肥製造 / 持続型物質生産 / 新規ATAD / 有機廃液の循環利用 / 複合微生物系制御
Outline of Research at the Start

本研究ではユニークな自家熱型高温好気消化において「ベンチュリ式通気撹拌の酸素供給特性と大きな剪断力により,好適な一部細菌群の優先的増殖と感受性菌群の死滅が誘起され,槽内環境変化と連動した細菌叢の劇的遷移が生じる」ことを証明するために下記の3点を明らかにする.
(1) 最終フェーズで優勢となる難培養性好熱性細菌群の顕微蛍光マニピュレーターを用いた液系分離と諸性質の解明
(2) ラボスケールで設計したベンチュリ式通気撹拌装置が主要分離菌と硝化菌,脱窒菌の増殖と複合微生物系形成に及ぼす影響の解明
(3) ベンチュリ式通気撹拌装置の酸素供給や機械的剪断力等の培養工学的特性の解明

Outline of Final Research Achievements

A unique autothermal thermophilic aerobic digestion (ATAD), showing distinctive bacterial community transitions and producing high-nitrogen content and pathogen-free organic liquid fertilizer was investigated. Investigation of a lab-scale gas-inducing system suggested that shear stress and oxygen supply system would affected the ATAD performances. Among 28 gene categories obtained by shotgun metagenomic analyses, abundance of four gene categories changed. A decrease in the phage-related gene category and the presence of bacteriolysis factors in secondary metabolism may explain the drastic change in bacterial community structure. A potential increase in the gene category in sporulation would be correlated with the deterioration of growth conditions and stabilization processes. In addition, unusually stable accumulation of ammonia throughout the process can be explained by the presence/absence of related metabolic genes belonging to the respective predominant bacterial families.

Academic Significance and Societal Importance of the Research Achievements

本研究の結果、し尿から衛生的で高窒素含量の有機液肥を製造する際の制御因子とそのユニークさの理由が明らかになった。学術的には複合微生物工学を呼称できる好例と考える。これにより、プロセスのパフォーマンス最大化と使用エネルギー・コストの最小化を目指すことができ、畜産し尿処理-リサイクル法としての開発、国内外の適地への導入促進を行う技術的素地ができた。普及により、資源循環型農業生産が広がり、持続社会の創成、SDGsの推進に大いに貢献することを期待したい。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (10 results)

All 2022 2020 2019

All Journal Article (9 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 8 results,  Open Access: 2 results) Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Promotion of a green economy with the palm oil industry for biodiversity conservation: A touchstone toward a sustainable bioindustry2022

    • Author(s)
      Sakai Kenji、Hassan Mohd Ali、Vairappan Charles Santhanaraju、Shirai Yoshihito
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 133 Issue: 5 Pages: 414-424

    • DOI

      10.1016/j.jbiosc.2022.01.001

    • NAID

      210000188863

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lab-scale autothermal thermophilic aerobic digestion can maintain and remove nitrogen by controlling shear stress and oxygen supply system2022

    • Author(s)
      Min Zhang, Yukihiro Tashiro, Yuya Asakura, Natsumi Ishida, Kota Watanabe, Siyuan Yue, Maruyama-Nakashita Akiko, and Kenji Saka
    • Journal Title

      ournal of Bioscience and Bioengineering

      Volume: 132 Pages: 293-301

    • NAID

      40022688018

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Clarification of the Dynamic Autothermal Thermophilic Aerobic Digestion Process using Metagenomic Analysis2022

    • Author(s)
      Natsumi Ishida, Yoshihisa Kawano, Ryo Fukui, Min Zhang, Yukihiro Tashiro, Kenji Sakai
    • Journal Title

      Microbiology Spectrum

      Volume: Accepted Pages: 1-7

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Isolation and selection of plant growth promoting endophytic bacteria associated with healthy hevea brasiliensis for use as plant growth promoters in rubber seedlings under salinity stress2020

    • Author(s)
      Kulwadee Khotchanalekha、Weerasak Saksirirat、Supat Isarangkool Na Ayutthaya、酒井謙二、田代幸寛、奥川友紀、Saowanit Tongpim
    • Journal Title

      Chiang Mai Journal of Science

      Volume: 47 Pages: 39-48

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Methane production from food waste via mesophilic anaerobic digestion with ethanol pre-fermentation: Methanogenic pathway and microbial community analyses2020

    • Author(s)
      Hui Zou、Ming Gao、Miao Yu、Wenyu Zhang、Shuang Zhang、Chuanfu Wu、田代幸寛、Qunhui Wang
    • Journal Title

      Bioresource Technology

      Volume: 297 Pages: 122450-122450

    • DOI

      10.1016/j.biortech.2019.122450

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Semi-hydrolysate of paper pulp without pretreatment enables a consolidated fermentation system with in situ product recovery for the production of butanol.2019

    • Author(s)
      Tao Zhao, Kento Yasuda, Yukihiro Tashiro, Rizki Fitria Darmayanti, Kenji Sakai & Kenji Sonomoto
    • Journal Title

      Bioresource Technology

      Volume: 278 Pages: 57-65

    • DOI

      10.1016/j.biortech.2019.01.043

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Dynamics of Microbial Populations Responsible for Biodegradation during the Full-Scale Treatment of Palm Oil Mill Effluent2019

    • Author(s)
      Diana Mohd-Nor, Norhayati Ramli, Siti Suhailah Sharuddin, Mohd Ali Hassan, Nurul Asyifah Mustapha, Hidayah Ariffin, 酒井謙二、田代幸寛、白井義人、前田憲成
    • Journal Title

      Microbes and Environments

      Volume: 34 Issue: 2 Pages: 121-128

    • DOI

      10.1264/jsme2.ME18104

    • NAID

      130007668529

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Mode and Structure of the Bacterial Community on Human Scalp Hair2019

    • Author(s)
      渡辺康太、西英二、田代幸寛、酒井謙二
    • Journal Title

      Microbes and Environments

      Volume: 34 Issue: 3 Pages: 252-259

    • DOI

      10.1264/jsme2.ME19018

    • NAID

      130007711470

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Highly efficient continuous acetone-butanol-ethanol production from mixed sugars without carbon catabolite repression.2019

    • Author(s)
      Takahiro Kihara, Takuya Noguchi, Yukihiro Tashiro, Kenji Sakai & Kenji Sonomoto
    • Journal Title

      Bioresource Technology Reports

      Volume: 印刷中 Pages: 100185-100185

    • DOI

      10.1016/j.biteb.2019.03.017

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] Investigation of Two Aeration Systems in the Dynamics of Physicochemical and Bacterial Characteristics in the ATAD Process2019

    • Author(s)
      Ming Zhang
    • Organizer
      The 10th China-Japan Joint Conference on Material Recycling and Waste Management
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research

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Published: 2019-04-18   Modified: 2023-01-30  

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