| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2021: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2019: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
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| Outline of Final Research Achievements |
Biosilicifying organisms form inorganic silica intracellularly. Long-chain polyamines (LCPAs) have been isolated from biosilica of phylogenetically distant biosilicifying organisms and are thought to be involved in biological silica formation. However, the biosynthetic pathway of LCPAs has not been clarified yet, which makes it difficult to investigate their physiological functions.
We previously found the genes that are essential for LCPA production in a mesophilic biosilicifying bacterium. In this study, we investigated the functions of the proteins encoded by these genes and found that one of them serves as the LCPA-synthesizing enzyme. We also developed a method to produce and isolate LCPAs from recombinant Escherichia coli cells. In vitro experiments showed that the purified LCPAs promotes silica formation from silicic acid.
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