| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2022: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Outline of Final Research Achievements |
Upstream open reading frames (uORFs) are elements found in the 5'-untranslated regions of eukaryotic transcripts that can affect translation of the downstream protein-coding sequences. In this study, we discovered that during translation of the uORF of an Arabidopsis gene, the translation complex containing the uORF-encoded nascent peptide directly senses magnesium to cause ribosome stalling, thereby repressing translation of the protein-coding sequence. Our results demonstrated that this magnesium-responsive translational regulation contributes to maintaining magnesium homeostasis in plant cells. Additionally, we identified a non-AUG-initiated uORF involved in the feedback regulation of the expression of a key enzyme of polyamine biosynthesis in plants.
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