| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2022: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2021: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2020: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Outline of Final Research Achievements |
In this study, we aimed to elucidate the fundamental mechanisms of piecemeal autophagy of chloroplasts and achieved the following results. (1) We established a method for isolating Rubisco-containing bodies (RCBs) and attempted to identify novel receptors, thereby gaining deeper insights into the selective recognition mechanism of chloroplast envelopes by autophagic isolation membranes. (2) We clarified the membrane dynamics involved in the formation and vacuolar transport of RCBs and plastid bodies, focusing on the dynamics of ATG8. (3) We elucidated the role of ATG8 isoforms in the RCB pathway through gene and protein expression analysis and the analysis of knockout mutants.
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