• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functional analysis and molecular design of glycosyltransferases in the biosynthesis of plant triterpenoids

Research Project

Project/Area Number 19H02921
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38060:Applied molecular and cellular biology-related
Research InstitutionOsaka University

Principal Investigator

MURANAKA TOSHIYA  大阪大学, 工学研究科, 教授 (60342862)

Co-Investigator(Kenkyū-buntansha) 平賀 勧  国立研究開発法人農業・食品産業技術総合研究機構, 作物研究部門, 上級研究員 (30332472)
石本 政男  国立研究開発法人農業・食品産業技術総合研究機構, 次世代作物開発研究センター, 研究領域長 (20355134)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2021: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords合成生物学 / ゲノム編集 / 代謝工学 / バイオプロダクション / 糖転移酵素 / 植物特化代謝物 / 代謝デザイン / 植物バイオテクノロジー / 植物特化代謝 / トリテルペノイド / グリチルリチン / ダイズ / 出芽酵母 / サポニン / セルロース合成酵素 / カンゾウ / 機能性物質
Outline of Research at the Start

自ら動くことができない植物は多種多様な化合物を作り、環境に適応して巧みに生存しています。植物がつくる化合物のうち、「トリテルペノイドサポニン」はさまざまな生理活性を有する重要な物質群です。本研究では、トリテルペノイドサポニンが植物の中でどのようにつくられ、どのような働きを示すか?またより高機能なトリテルペノイドサポニンを人工的にデザインできないかについて研究を行います。これにより将来、より環境に強い植物を作り出すことや、健康機能性が高い植物を作ることが期待できます。

Outline of Final Research Achievements

Triterpenoids, which have a 30-carbon skeleton, are biosynthesized as triterpenoid saponins with diverse biological activities, undergoing site-specific modification by glycosyltransferases. In this study, one of a group of enzymes similar to cellulose synthases was first identified as an enzyme that transfers UDP-glucuronic acid to the C-3 position of oleanane-type triterpenoids, and was named CSyGT. Furthermore, CSyGT is localized in the endoplasmic reticulum, and by introducing a total of seven plant-derived genes involved in glycyrrhizin biosynthesis in budding yeast, a biosynthetic system up to glycyrrhizin could be reconstructed.

Academic Significance and Societal Importance of the Research Achievements

これまでトリテルペノイドにUDP糖を転移する酵素は、UDP糖依存型糖転移酵素(UDG)に分類される酵素群であると信じられてきたが、発想を転換し、遺伝子共発現解析と、より広義な糖転移酵素群に検索枠を広げた結果、これまで予想もつかなかった、CSyGTがオレナン型トリテルペノイドアグリコンのC-3位にグルクロン酸を転移する酵素であるという、画期的な成果を得ることができた学術的意義は大きい。また、本酵素遺伝子を用いてゲノム編集技術を駆使することにより、酵母のみならず植物細胞・組織によるバイオプロダクションの道筋を示すことができ社会的意義も大きい。

Report

(3 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (41 results)

All 2021 2020 2019 Other

All Int'l Joint Research (4 results) Journal Article (10 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 10 results,  Open Access: 6 results) Presentation (23 results) (of which Int'l Joint Research: 6 results,  Invited: 10 results) Remarks (1 results) Patent(Industrial Property Rights) (3 results) (of which Overseas: 1 results)

  • [Int'l Joint Research] エクアドル IKIAM(その他の国・地域)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] ITB(インドネシア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] エクアドル IKIAM(その他の国・地域)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ITB(インドネシア)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Allylic Hydroxylation Activity Is a Source of Saponin Chemodiversity in the Genus Glycyrrhiza2021

    • Author(s)
      Fanani Much Z、Sawai Satoru、Seki Hikaru、Ishimori Masato、Ohyama Kiyoshi、Fukushima Ery O、Sudo Hiroshi、Saito Kazuki、Muranaka Toshiya
    • Journal Title

      Plant and Cell Physiology

      Volume: in press Issue: 2 Pages: 262-271

    • DOI

      10.1093/pcp/pcaa173

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Insights into the diversification of subclade IVa bHLH transcription factors in Fabaceae2021

    • Author(s)
      Suzuki Hayato、Seki Hikaru、Muranaka Toshiya
    • Journal Title

      BMC Plant Biology

      Volume: 21 Issue: 1 Pages: 109-109

    • DOI

      10.1186/s12870-021-02887-w

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A cellulose synthase-derived enzyme catalyses 3-O-glucuronosylation in saponin biosynthesis2020

    • Author(s)
      Chung Soo Yeon、Seki Hikaru、Fujisawa Yukiko、Shimoda Yoshikazu、Hiraga Susumu、Nomura Yuhta、Saito Kazuki、Ishimoto Masao、Muranaka Toshiya
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 5664-5664

    • DOI

      10.1038/s41467-020-19399-0

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Production of the bioactive plant‐derived triterpenoid morolic acid in engineered Saccharomyces cerevisiae2020

    • Author(s)
      Srisawat Pisanee、Yasumoto Shuhei、Fukushima Ery O.、Robertlee Jekson、Seki Hikaru、Muranaka Toshiya
    • Journal Title

      Biotechnology and Bioengineering

      Volume: 117 Issue: 7 Pages: 2198-2208

    • DOI

      10.1002/bit.27357

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] ダイズサポニンの生合成と機能 (特集 ダイズ成分の機能と育種)2020

    • Author(s)
      石本政男、塚本知玄
    • Journal Title

      アグリバイオ

      Volume: 4 Pages: 1049-1053

    • NAID

      40022425837

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of oxidosqualene cyclases from the medicinal legume tree Bauhinia forficata: a step toward discovering preponderant α-amyrin-producing activity2019

    • Author(s)
      Srisawat P, Fukushima EO, Yasumoto S, Robertlee J, Suzuki H, Seki H, Muranaka T
    • Journal Title

      New Phytol.

      Volume: 224 Issue: 1 Pages: 352-366

    • DOI

      10.1111/nph.16013

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Functional specialization of UDP-glycosyltransferase 73P12 in licorice to produce a sweet triterpenoid saponin, glycyrrhizin2019

    • Author(s)
      Nomura Y, Seki H, Suzuki T, Ohyama K, Mizutani M, Kaku T, Tamura K, Ono E, Horikawa M, Sudo H, Hayashi H, Saito K, Muranaka T
    • Journal Title

      Plant J.

      Volume: 99 Issue: 6 Pages: 1127-1143

    • DOI

      10.1111/tpj.14409

    • NAID

      120006779844

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lotus japonicus triterpenoid profile and characterization of CYP716A51 and LjCYP93E1 genes involved in its biosynthesis in planta2019

    • Author(s)
      Suzuki H, Fukushima EO, Shimizu Y, Seki H, Fujisawa Y, Ishimoto M, Osakabe K, Osakabe Y, Muranaka T
    • Journal Title

      Plant Cell Physiol.

      Volume: 60 Pages: 2496-2509

    • DOI

      10.1093/pcp/pcz145

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular basis of C-30 product regioselectivity of legume oxidasesinvolved in high-value triterpenoid biosynthesis2019

    • Author(s)
      Fanani MZ, Fukushima EO, Sawai S, Tang J, Ishimori M, Sudo H, Ohyama K, Seki H, Saito K, Muranaka T
    • Journal Title

      Front. Plant Sci.

      Volume: 10 Pages: 1520-1520

    • DOI

      10.3389/fpls.2019.01520

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structure?Activity Relationships of Pentacyclic Triterpenoids as Inhibitors of Cyclooxygenase and Lipoxygenase Enzymes2019

    • Author(s)
      Vo Nhu Ngoc Quynh、Nomura Yuhta、Muranaka Toshiya、Fukushima Ery Odette
    • Journal Title

      Journal of Natural Products

      Volume: 82 Issue: 12 Pages: 3311-3320

    • DOI

      10.1021/acs.jnatprod.9b00538

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 甘草はどのようにして甘いサポニンの生産能を獲得したのか?2021

    • Author(s)
      村中俊哉、關光
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 共発現解析によるサポニンの生合成に関わる新規配糖化酵素の発見2021

    • Author(s)
      石本政男, 藤澤由紀子, Soo Yeon Chung, 關 光, 下田 宜司, 平賀 勧, 野村勇太, 斉藤和季, 村中俊哉
    • Organizer
      日本育種学会第139会講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Redesign of triterpenoid biosynthetic pathway in plants by genome editing2020

    • Author(s)
      Muranaka T
    • Organizer
      2020 International Conference of the Korean Society of Plant Biologists
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Plant Genome Engineering to Minimize Anti-Nutritional Metabolites2020

    • Author(s)
      Muranaka T
    • Organizer
      Institute of Food Technologists
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] マメ科におけるサブクレードIVa bHLH転写因子の多様化への洞察2020

    • Author(s)
      鈴木隼人、關光、村中俊哉
    • Organizer
      第84回日本植物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ミヤコグサのベツリン酸生合成を制御するbHLH型転写因子の同定2020

    • Author(s)
      鈴木隼人、高橋宏和、福島エリオデット、關光、中園幹夫、村中俊哉
    • Organizer
      第30回イソプレノイド研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] トリテルペノイドのダイズ二次通気組織における生理機能の解明2020

    • Author(s)
      安保知里、牛来智香、柳川麻子、加賀秋人、鈴木隼人、關光、村中俊哉、中園幹夫、高橋宏和
    • Organizer
      第27回育種学会中部地区談話会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ダイズにおけるルペオール合成酵素の機能解析2020

    • Author(s)
      安保知里、牛来智香、柳川麻子、鈴木隼人、富崎由佳理、關光、加賀秋人、村中俊哉、中園幹夫、高橋宏和
    • Organizer
      第138回日本育種学会秋季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] トリテルペノイドサポニン生合成におけるセルロースシンターゼライクの機能解析2020

    • Author(s)
      Soo Yeon Chung, 關光、村中俊哉
    • Organizer
      第30回 イソプレノイド研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] A cellulose synthase-derived enzyme catalyses 3-O-glucuronosylation in saponin biosynthesis2020

    • Author(s)
      Soo Yeon Chung, Hikaru Seki, Toshiya Muranaka
    • Organizer
      日本植物生理学会大阪大会サテライトシンポジウム
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ミヤコグサのトリテルペンプロファイルおよび植物体におけるCYP716A51とLjCYP93E1酵素遺伝子機能の解析2020

    • Author(s)
      鈴木隼人、福島エリオデット、清水裕子、關光、藤澤由紀子、石本政男、刑部敬史、刑部祐里子、村中俊哉
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 植物テルペノイド生合成の代謝エンジニアリング-生薬原料を酵母で創る、作物で創る2019

    • Author(s)
      村中俊哉
    • Organizer
      日本生薬学会北海道支部第43回例会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 植物テルペノイド生合成の代謝エンジニアリング-生薬原料を酵母で創る、作物で創る2019

    • Author(s)
      村中俊哉
    • Organizer
      日本試薬協会西部地区講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Genome editing to improve plant specialized metabolite pathway2019

    • Author(s)
      Toshiya Muranaka
    • Organizer
      Joint Symposium on Plant Science and Products 2019 (ITB, Indonesia)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Genome editing to improve plant specialized metabolite pathway2019

    • Author(s)
      Toshiya Muranaka
    • Organizer
      World Class Professor, International Seminar (ITB, Indonesia)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 植物テルペノイド代謝多様性の分子基盤と代謝工学2019

    • Author(s)
      村中俊哉
    • Organizer
      日本植物細胞分子生物学会年会(京都大会)
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ミヤコグサのウルサン型トリテルペノイド生合成遺伝子の同定2019

    • Author(s)
      鈴木隼人、福島エリオデット、關光、村中俊哉
    • Organizer
      日本植物細胞分子生物学会年会(京都大会)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Bioinformatics platform and in vivo functional analysis to reveal the key amino acid of CYP716 family in triterpenoid biosynthesis2019

    • Author(s)
      Jutapat Romsuk, Ery Odette Fukushima, Shuhei Yasumoto, Hikaru Seki, Toshiya Muranaka
    • Organizer
      日本植物細胞分子生物学会年会(京都大会)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Comparative analysis of plant NADPH-cytochrome P450 reductase towards heterologous triterpenoid production in transgenic yeast2019

    • Author(s)
      Pramesti Istiandari, Ery Odette Fukushima, Shuhei Yasumoto, Hikaru Seki, Toshiya Muranaka
    • Organizer
      日本植物細胞分子生物学会年会(京都大会)
    • Related Report
      2019 Annual Research Report
  • [Presentation] ヒヨコマメにおけるソヤサポゲノールB生合成酵素遺伝子の同定と機能解析2019

    • Author(s)
      橋本 彩希、Fanani Much Zaenal1、安本 周平、關 光、福島 エリオデット、村中 俊哉
    • Organizer
      日本植物細胞分子生物学会年会(京都大会)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 植物・植物組織培養による有用物質生産の新潮流2019

    • Author(s)
      村中俊哉
    • Organizer
      BioJapan2019
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Redesign of terpenoid biosynthetic pathway in plant by genome editing toward human health2019

    • Author(s)
      Toshiya MURANAKA
    • Organizer
      International Meeting on Plant Secondary Metabolism (Jingzhou, China)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 共発現解析に基づくダイズサポニン配糖化酵素の同定2019

    • Author(s)
      Krishnamurthy Panneerselvam, 藤澤由紀子, Soo-Yeon Chung, Hae-Reon Son, 下田宜司, 平賀勧, 加賀秋人, 穴井豊昭, 塚本知玄, 關光, 村中俊哉, 石本政男
    • Organizer
      日本育種学会2019年秋季大会(136回)
    • Related Report
      2019 Annual Research Report
  • [Remarks] 大阪大学大学院工学研究科 村中研究室

    • URL

      http://www.bio.eng.osaka-u.ac.jp/pl/index.html

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Patent(Industrial Property Rights)] グルクロン酸転移酵素、それをコードする遺伝子及びその利用方法2020

    • Inventor(s)
      石本政男、平賀勧、佐藤由紀子、村中俊哉、關光、チョンスヨン
    • Industrial Property Rights Holder
      石本政男、平賀勧、佐藤由紀子、村中俊哉、關光、チョンスヨン
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2020 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 植物形質転換体2020

    • Inventor(s)
      村中俊哉、關光、石本政男、佐藤由紀子、高木恭子、矢野亮一
    • Industrial Property Rights Holder
      村中俊哉、關光、石本政男、佐藤由紀子、高木恭子、矢野亮一
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-004640
    • Filing Date
      2020
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] グルクロン酸転移酵素、それをコードする遺伝子及びその利用方法2019

    • Inventor(s)
      石本政男、平賀勧、佐藤由紀子、村中俊哉、關光、チョン ス ヨン
    • Industrial Property Rights Holder
      石本政男、平賀勧、佐藤由紀子、村中俊哉、關光、チョン ス ヨン
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-190060
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report

URL: 

Published: 2019-04-18   Modified: 2025-03-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi