• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of the molecular mechanism how deficiency of deglycosylation in the cytosol causes abnormalities in organism.

Research Project

Project/Area Number 19H02926
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38060:Applied molecular and cellular biology-related
Research InstitutionTokyo Metropolitan Institute of Medical Science

Principal Investigator

YOSHIDA Yukiko  公益財団法人東京都医学総合研究所, 基礎医科学研究分野, 主席研究員 (90271543)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2021: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2020: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
KeywordsNGLY1 / F-boxタンパク質 / ユビキチンリガーゼ / 糖鎖 / タンパク質品質管理 / プロテアソーム / NGLY1欠損症 / Fbx2/Fbxo6 / 小胞体関連分解 / Fbs2 / 細胞質糖鎖
Outline of Research at the Start

小胞体で正しい立体構造形成に失敗した糖タンパク質は、細胞質へ逆行輸送され、ユビキチン化を受け、細胞質にある糖鎖除去酵素NGLY1によりN型糖鎖をはずされた後、プロテアソームにより分解を受ける。NGLY1欠損症はNGLY1の変異による希少疾患であり、NGLY1のKOマウスは胎生致死となる。代表者は、細胞質にある糖鎖認識ユビキチンリガーゼの基質認識サブユニットFbs2の遺伝子欠損がNGLY1-KOマウスの胎生致死を回避し、全く正常に生育することを見出した。本研究は、細胞質におけるN型糖鎖除去システムの破綻が、Fbs2依存的にマウスの致死性を引き起こす分子機構を解明することを目的とする。

Outline of Final Research Achievements

Peptide:N-glycanase (NGLY1) is responsible for removing N-glycans from glycoprotein in the cytosol. Mutations in the human NGLY1 gene cause multisystemic symptoms, but the molecular mechanism underlying pathogenesis remains unknown. In mice, Ngly1 knockout (KO) is embryonic lethal. We found that deletion of Fbs2, which encodes a SCF-type ubiquitin ligase subunit that recognizes N-glycans, rescued the lethality and progressive defects in Ngly1-KO mice. In NGLY1-KO cells, Fbs2 expression caused cytotoxicity. Nrf1, an endoplasmic reticulum-associated transcriptional factor for the proteasome, was ubiquitinated by SCFFbs2 in NGLY1-KO cells, resulting in its retention in the cytosol. The cytotoxicity caused by Fbs2 expression was restored by overexpression of glycan-less Nrf1 mutations, or by the deletion of Nrf1 in NGLY1-KO cells. Thus, the proteasome dysfunction caused by accumulation of NRF1 ubiquitinated by SCFFbs2 causes cytotoxicity in NGLY1 deficiency.

Academic Significance and Societal Importance of the Research Achievements

多くの多細胞生物において、細胞質でN型糖鎖を除去する酵素NGLY1遺伝子の不活化変異は様々な不具合を生じるが、その機序は不明であった。今回の研究成果は、細胞質における2つのN型糖鎖関連タンパク質NGLY1とFbs2の遺伝的相互作用に着目し、細胞質でNGLY1により糖鎖が除去されない場合にどのように個体に異常を引き起こすのか、その分子機構を明確に示した。
また、Fbs2がこれまで治療法のなかったヒト希少疾患NGLY1欠損症の有望な治療ターゲットとなり得ることを示した。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (15 results)

All 2022 2021 2020 2019 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results,  Open Access: 5 results) Presentation (7 results) (of which Int'l Joint Research: 4 results,  Invited: 5 results) Remarks (1 results)

  • [Journal Article] Ever-expanding NGLY1 biology.2022

    • Author(s)
      Tadashi Suzuki, Yukiko Yoshida
    • Journal Title

      J. Biochem.

      Volume: 171 Issue: 2 Pages: 141-143

    • DOI

      10.1093/jb/mvab134

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Loss of peptide: N -glycanase causes proteasome dysfunction mediated by a sugar-recognizing ubiquitin ligase2021

    • Author(s)
      Yoshida Yukiko、Asahina Makoto、Murakami Arisa、Kawawaki Junko、Yoshida Meari、Fujinawa Reiko、Iwai Kazuhiro、Tozawa Ryuichi、Matsuda Noriyuki、Tanaka Keiji、Suzuki Tadashi
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 118 Issue: 27

    • DOI

      10.1073/pnas.2102902118

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] MIND bomb 2 prevents RIPK1 kinase activity-dependent and -independent apoptosis through ubiquitylation of cFLIPL2021

    • Author(s)
      Nakabayashi Osamu、Takahashi Hirotaka、Moriwaki Kenta、Komazawa-Sakon Sachiko、Ohtake Fumiaki、Murai Shin、Tsuchiya Yuichi、Koyahara Yuki、Saeki Yasushi、Yoshida Yukiko、Yamazaki Soh、Tokunaga Fuminori、Sawasaki Tatsuya、Nakano Hiroyasu
    • Journal Title

      Communications Biology

      Volume: 4 Issue: 1 Pages: 80-80

    • DOI

      10.1038/s42003-020-01603-y

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lectin-Type Ubiquitin Ligase Subunits: Fbs Proteins and Their Applications for Use2020

    • Author(s)
      Yukiko Yoshida
    • Journal Title

      Methods Mol Biol.

      Volume: 2132 Pages: 215-224

    • DOI

      10.1007/978-1-0716-0430-4_22

    • ISBN
      9781071604298, 9781071604304
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High expression of FOXM1 critical for sustaining cell proliferation in mitochondrial DNA-less liver cancer cells2020

    • Author(s)
      Masato Higurashi, Tsuyoshi Maruyama, Yusuke Nogami, Fumihiro Ishikawa, Yukiko Yoshiea, Kazuhori Mori, Kenichi Fujita, Motoko Shibanuma.
    • Journal Title

      Exp. Cell Res.

      Volume: 389 Pages: 11889-11889

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sugar-Recognizing Ubiquitin Ligases: Action Mechanisms and Physiology.2019

    • Author(s)
      Yoshida Y, Mizushima T, Tanaka K.
    • Journal Title

      Front Physiol.

      Volume: 10 Pages: 104-104

    • DOI

      10.3389/fphys.2019.00104

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Detection of ubiquitination activity and identification of ubiquitinated substrates using TR-TUBE2019

    • Author(s)
      Yoshida, Y., Saeki, Y., Tsuchiya, H., and Tanaka, K.
    • Journal Title

      Methods Enzymol

      Volume: 618 Pages: 135-147

    • DOI

      10.1016/bs.mie.2018.12.032

    • ISBN
      9780128163597
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] プロテアソーム転写誘導因子NRF1の分解機構の解析2021

    • Author(s)
      木下大生、吉田雪子、松田憲之、田中啓二
    • Organizer
      第94回日本生化学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] SCFFBS2/FBXO6 causes proteasome dysfuncition in NGLY1 deficiency.2021

    • Author(s)
      Yukiko Yoshida
    • Organizer
      8thGlobal NGLY1 Conference
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Ubiquitination of alpha-synuclein is not necessary for fibril formation and transmission in cultured cells.2021

    • Author(s)
      Yukiko Yoshida, Arisa Murakami, Junko Kawawaki, Meari Yoshida, Katsumata, Ryu, Masato Hasegawa
    • Organizer
      Pacifichem2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional genetic interaction between NGLY1 and a glycoprotein-specific F-box protein FBS2.2020

    • Author(s)
      吉田雪子
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Functional genetic interaction between Ngly1 and a lectin-type ubiquitin ligase in mice2020

    • Author(s)
      Yukiko Yoshida
    • Organizer
      RIKEN-International Symposim 'Glyco-lipidologue Symposium'
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Loss of a lectin-type E3 ligase gene rescues the lethality and defects of NGLY-KO mice.2019

    • Author(s)
      Yukiko Yoshida
    • Organizer
      CIFAR Genetic Networks Program Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Ngly1と糖鎖認識ユビキチンリガーゼSCFFbxo6のマウスにおける遺伝的相互作用2019

    • Author(s)
      吉田雪子
    • Organizer
      第38回日本糖質学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Remarks] 研究内容紹介ページ

    • URL

      http://www.igakuken.or.jp/protein/jpn/research/yoshida-team.html

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report 2019 Annual Research Report

URL: 

Published: 2019-04-18   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi