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Functional analysis of the proteins for the endoplasmic reticulum in the intracellular storage of substance in rice seed and the use in breeding

Research Project

Project/Area Number 19H02933
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39010:Science in plant genetics and breeding-related
Research InstitutionKyushu University

Principal Investigator

Kumamaru Toshihiro  九州大学, 農学研究院, 教授 (00284555)

Co-Investigator(Kenkyū-buntansha) 久保 貴彦  九州大学, 農学研究院, 准教授 (00370148)
牛島 智一  摂南大学, 農学部, 講師 (50815058)
藤田 直子  秋田県立大学, 生物資源科学部, 教授 (90315599)
Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2022: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2021: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2020: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywords貯蔵タンパク質 / イネ / 種子 / デンプン / オルガネラ / 機能解析 / 澱粉 / 突然変異
Outline of Research at the Start

貯蔵タンパク質の細胞内輸送や蓄積に異常をきたしている突然変異体では、貯蔵タンパク質組成の変化とともに異常な澱粉粒の形成が認められる。植物の物質貯蔵機能の理解のために、小胞体機能タンパク質の貯蔵タンパク質の輸送・蓄積と澱粉合成代謝における機能、小胞体機能タンパク質と加工特性等との関係を解明する。本研究の成果は、優れた加工特性や新奇機能性を有するコメの開発に寄与する。

Outline of Final Research Achievements

The causal genes of esp1, lcrp, and esp3 mutations for the prolamine accumulation encoded the eRF1, SHMT4, and the factor for the amino acid metabolism, respectively. The causal gene of glup7 mutation accumulating the proglutelin encoded the factor for the transportation of the proglutelin from the endoplasmic reticulum (ER). The starch synthesis related enzymes and the starch properties in the seed of mutants for the storage proteins were analyzed, resulting that those in mutants didn't indicate the significant difference compared to the wild type. Koshihikari breeding line with the esp2 mutant allele, which accumulats the proglutelin within the ER and defects the PDIL1-1, indicated the good processing properties for food.

Academic Significance and Societal Importance of the Research Achievements

植物の物質貯蔵機能の理解のために、小胞体関連機能タンパク質(以下小胞体タンパク質)の貯蔵タンパク質の輸送・蓄積と澱粉合成代謝における機能、小胞体タンパク質と加工特性等との関係を明らかにすることは重要である。本研究の成果は、植物における種子貯蔵タンパク質の輸送・蓄積系のモデル構築を担うと共に、植物の澱粉、脂質等の物質貯蔵における小胞体タンパク質の機能解明の一助となる。加えて、イネ種子の小胞体タンパク質と加工特性及び機能性付与との関係が明らかになり、優れた加工特性や新奇機能性を有するコメの開発に寄与する。

Report

(4 results)
  • 2023 Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (17 results)

All 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (1 results) Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (10 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Int'l Joint Research] ワシントン州立大学(米国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Whole-Genome Sequencing of Rice Mutant Library Members Induced by N-Methyl-N-Nitrosourea Mutagenesis of Fertilized Egg Cells2022

    • Author(s)
      Kubo Takahiko、Yamagata Yoshiyuki、Matsusaka Hiroaki、Toyoda Atsushi、Sato Yutaka、Kumamaru Toshihiro
    • Journal Title

      Rice

      Volume: 15 Issue: 1 Pages: 38-38

    • DOI

      10.1186/s12284-022-00585-1

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic Background Negates Improvements in Rice Flour Characteristics and Food Processing Properties Caused by a Mutant Allele of the PDIL1-1 Seed Storage Protein Gene2022

    • Author(s)
      Hori Kiyosumi、Okunishi Tomoya、Nakamura Kenji、Iijima Ken、Hagimoto Masahiro、Hayakawa Katsuyuki、Shu Koka、Ikka Takashi、Yamashita Hiroto、Yamasaki Masanori、Takeuchi Yoshinobu、Koyama Shota、Tsujii Yoshimasa、Kayano Toshiaki、Ishii Takuro、Kumamaru Toshihiro、Kawagoe Yasushi、Yamamoto Toshio
    • Journal Title

      Rice

      Volume: 15 Issue: 1 Pages: 13-13

    • DOI

      10.1186/s12284-022-00560-w

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Zipcode RNA-Binding Proteins and Membrane Trafficking Proteins Cooperate to Transport Glutelin mRNAs in Rice Endosperm2020

    • Author(s)
      Li Tian, Kelly A. Doroshenk, Laining Zhang, Masako Fukuda, Haruhiko Washida, Toshihiro Kumamaru, Thomas Okita
    • Journal Title

      Plant Cell

      Volume: 32 Issue: 8 Pages: 2566-2581

    • DOI

      10.1105/tpc.20.00111

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] mRNA Localization in Plant Cells2020

    • Author(s)
      Tian L, Chou HL, Fukuda M, Kumamaru T, Okita TW.
    • Journal Title

      Plant Physiology

      Volume: 182 Issue: 1 Pages: 97-109

    • DOI

      10.1104/pp.19.00972

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] イネ変異体オンラインスクリーニングシステムの構築と公開 ,2023

    • Author(s)
      久保 貴彦 , 山形 悦透 , 松坂 弘明 , 佐藤 豊 ,熊丸 敏博
    • Organizer
      日本育種学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Serine hydroxymethyltransferase (SHMT)はイネ種子のシステイン高含有貯蔵タンパク質の合成に関与する2021

    • Author(s)
      福田真子,松坂弘明,長嶺愛,熊丸敏博
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] イネのTILLING 変異系統を用いたOsHKT1;4 Na+ 輸送体の塩ストレス下における生理機能の解明2020

    • Author(s)
      河村敏貴,小林奈通子,グエンタンハオ,石川亮,且原真木,田野井慶太朗,松坂弘明,熊丸敏博
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Golgi Transport 1 (GOT1B)は貯蔵タンパク質RNAの特定小胞体領域への局在及びグルテリンとα-グロブリンの小胞体からの効率的な輸送に必要である2020

    • Author(s)
      福田真子,熊丸敏博,Thomas W. Okita
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] イネデンプン構造変異系統の胚乳特性解析および原因遺伝子探索2020

    • Author(s)
      永松 志朗,和田 卓也,松島 良,藤田 直子,三浦 聡子,クロフツ 尚子,保坂 優子,熊丸 敏博
    • Organizer
      日本育種学会第137回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 超軟質米の作出に向けた Isoamylase1活性低下変異の単離および解析2019

    • Author(s)
      中村哲洋, Tho Nguyen, 久保貴彦, 熊丸敏博
    • Organizer
      日本育種学会第14回九州育種談話会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Identification of genomic region harboring Endosperm Storage Protein (ESP) 3 gene regulating the Cysteine-rich prolamine by MutMap.2019

    • Author(s)
      Tran Hong Quan, Hiroaki Matsusaka, Tomokazu Ushijima,Takahiko Kubo, Toshirhiro Kumamaru
    • Organizer
      日本育種学会第14回九州育種談話会
    • Related Report
      2019 Annual Research Report
  • [Presentation] GOT1B is required for localization of storage protein RNA and for export of proglutelin from ER2019

    • Author(s)
      Masako Fukuda, Toshihiro Kumamaru, Thomas W. Okita
    • Organizer
      American Society of Plant Biologist, Plant Biology 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of a new gene involved in starch metabolism of rice2019

    • Author(s)
      グエン テイ フオング トー, 中村 哲洋, 熊丸 敏博, 久保 貴彦
    • Organizer
      日本育種学会第136回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 難消化性澱粉を保有するイネ澱粉構造変異系統の胚乳特性解析2019

    • Author(s)
      和田卓也, 松島良, 藤田直子, 三浦聡子, クロフツ尚子, 保坂優子,永松志朗, 熊丸敏博
    • Organizer
      第68回日本応用糖質科学会大会
    • Related Report
      2019 Annual Research Report
  • [Remarks] 九州大学研究者情報(熊丸敏博)

    • URL

      https://hyoka.ofc.kyushu-u.ac.jp/search/details/K001628/research.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] 九州大学農学研究院 植物遺伝子資源分野

    • URL

      http://www.agr.kyushu-u.ac.jp/lab/pgr/

    • Related Report
      2019 Annual Research Report

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Published: 2019-04-18   Modified: 2025-01-30  

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