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Development of a method for identification of QTLs for photosynthesis in rice by direct measurement of leaf gas exchange

Research Project

Project/Area Number 19H02939
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39020:Crop production science-related
Research InstitutionTokyo University of Agriculture and Technology (2020-2022)
Ibaraki University (2019)

Principal Investigator

Adachi Shunsuke  東京農工大学, (連合)農学研究科(研究院), 准教授 (30717103)

Co-Investigator(Kenkyū-buntansha) 松岡 信  福島大学, 食農学類附属発酵醸造研究所, 特任教授 (00270992)
田中 佑  京都大学, 農学研究科, 助教 (50634474)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2022: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2021: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
KeywordsMIC-100 / イネ / CO2同化速度 / GWAS / ゲノム / 光合成 / 閉鎖系 / ジャポニカパネル / 品種間差 / 在来品種 / 新型測定装置 / QTL
Outline of Research at the Start

葉の光合成能力に関わる遺伝要因の解明は作物生産性の向上のために重要であるが、従来のQTL解析手法とガス交換測定手法には網羅性と迅速性に欠点があり、研究の進展を妨げる要因となっていた。そこで本研究では、QTL検出の新手法である網羅性と迅速性の高いゲノムワイド関連解析と、申請者らが最近開発した新型迅速光合成測定装置を組み合わせることによって、イネ光合成能力の自然変異に関わる原因遺伝子の同定を目指す。本研究成果によって、光合成の自然変異遺伝子の解明が大きく進むと期待される。

Outline of Final Research Achievements

Using the new rapid photosynthesis measurement system MIC-100, which reduces the measurement time per leaf to less than 1/10 of the conventional method, we analyzed the variation in CO2 assimilation rates of 198 rice varieties bred in Japan. The results showed that there is a wide range of variation in the CO2 assimilation rate of Japanese rice varieties comparable to the world panel of rice varieties, and that the CO2 assimilation rate has not been improved during the modern breeding process in Japan. Genome wide association study with integrated three years of measurement data identified candidate genomic regions on chromosomes 1, 8, and 11. Of these, the chromosome 1 region was in close proximity to the QTL detected in the Nihonbaru/Hajikuroho F2 population.

Academic Significance and Societal Importance of the Research Achievements

かつてない大規模な光合成評価により、日本で育成されたイネの光合成速度の多様性が網羅的に示された。特に日本のイネを利用した光合成改良の可能性が高いことが示された。またMIC-100を利用した光合成解析とGWASの融合は、CO2同化速度に関わるゲノム領域をきわめて省力的に特定できることが明らかとなった。MIC-100によるガス交換直接測定法は、近年開発された間接評価法とともに作物光合成の制御因子の解明に役立つと期待される。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (23 results)

All 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (3 results) Journal Article (12 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 12 results,  Open Access: 6 results) Presentation (6 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Int'l Joint Research] University of Toronto(カナダ)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] University of Toronto(カナダ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] トロント大学(カナダ)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] 葉のCO2同化速度の簡便迅速測定システムの開発2023

    • Author(s)
      本田爽太郎・大久保智司・新家寿建・秋山重之・青木直史・田中佑・安達俊輔
    • Journal Title

      日本作物学会紀事

      Volume: -

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Towards improved dynamic photosynthesis in C3 crops by utilising natural genetic variation2022

    • Author(s)
      Sakoda Kazuma、Adachi Shunsuke、Yamori Wataru、Tanaka Yu
    • Journal Title

      Journal of Experimental Botany

      Volume: not determined Issue: 10 Pages: 3109-3121

    • DOI

      10.1093/jxb/erac100

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anisohydric characteristics of a rice genotype ‘ <scp>ARC</scp> 11094’ contribute to increased photosynthetic carbon fixation in response to high light2022

    • Author(s)
      Taniyoshi Kazuki、Tanaka Yu、Adachi Shunsuke、Shiraiwa Tatsuhiko
    • Journal Title

      Physiologia Plantarum

      Volume: 174 Issue: 6

    • DOI

      10.1111/ppl.13825

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Evolution of C4 Photosynthesis in Flaveria (Asteraceae): Insights from the Flaveria linearis Complex2022

    • Author(s)
      Adachi Shunsuke、Stata Matt、Martin Duncan G、Cheng Shifeng、Liu Hongbing、Zhu Xin-Guang、Sage Rowan F
    • Journal Title

      Plant Physiology

      Volume: 191 Issue: 1 Pages: 233-251

    • DOI

      10.1093/plphys/kiac467

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Two bHLH transcription factors affect sprouting by regulating the level of ABA2022

    • Author(s)
      Yoshida Hideki、Matsuoka Makoto
    • Journal Title

      Nature Genetics

      Volume: 54 Issue: 12 Pages: 1772-1773

    • DOI

      10.1038/s41588-022-01238-1

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alternating Red/Blue Light Increases Leaf Thickness and Mesophyll Cell Density in the Early Growth Stage, Improving Photosynthesis and Plant Growth in Lettuce2021

    • Author(s)
      OHTAKE Noriko、JU Yao、ISHIKURA Masaharu、SUZUKI Hiroshi、ADACHI Shunsuke、YAMORI Wataru
    • Journal Title

      Environment Control in Biology

      Volume: 59 Issue: 2 Pages: 59-67

    • DOI

      10.2525/ecb.59.59

    • NAID

      130008031277

    • ISSN
      1880-554X, 1883-0986
    • Year and Date
      2021-04-01
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] MIC-100, a new system for high-throughput phenotyping of instantaneous leaf photosynthetic rate in the field2021

    • Author(s)
      Yu Tanaka, Kazuki Taniyoshi, Ayumu Imamura, Ryo Mukai, Shun Sukemura, Kazuma Sakoda, Shunsuke Adachi
    • Journal Title

      Functional Plant Biology

      Volume: 49 Issue: 6 Pages: 1-9

    • DOI

      10.1071/fp21029

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Maintaining higher leaf photosynthesis after heading stage could promote biomass accumulation in rice2021

    • Author(s)
      Honda Sotaro、Ohkubo Satoshi、San Nan Su、Nakkasame Anothai、Tomisawa Kazuki、Katsura Keisuke、Ookawa Taiichiro、Nagano Atsushi J.、Adachi Shunsuke
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 7579-7579

    • DOI

      10.1038/s41598-021-86983-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Continuous estimation of rice (Oryza sativa (L.)) canopy transpiration realized by modifying the heat balance model2021

    • Author(s)
      Kondo Rintaro、Tanaka Yu、Katayama Hiroto、Homma Koki、Shiraiwa Tatsuhiko
    • Journal Title

      Biosystems Engineering

      Volume: 204 Pages: 294-303

    • DOI

      10.1016/j.biosystemseng.2021.01.016

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of Upper-Limb Assist Suit for Reduction Physical Load in Leaf Photosynthesis Measurement2020

    • Author(s)
      ARAIE Toshitake, IKEDA Tomozumi, KAKIMOTO Akira, ADACHI Shunsuke
    • Journal Title

      International Journal of Modeling and Optimization

      Volume: 10(6) Pages: 185-190

    • DOI

      10.7763/ijmo.2020.v10.768

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rice Cultivar Takanari Has Higher Photosynthetic Performance Under Fluctuating Light Than Koshihikari, Especially Under Limited Nitrogen Supply and Elevated CO22020

    • Author(s)
      Ohkubo Satoshi、Tanaka Yu、Yamori Wataru、Adachi Shunsuke
    • Journal Title

      Frontiers in Plant Science

      Volume: 11 Pages: 1308-1308

    • DOI

      10.3389/fpls.2020.01308

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic determination for source capacity to support breeding of high-yielding rice (Oryza sativa)2020

    • Author(s)
      Shunsuke Adachi, Satoshi Ohkubo, Nan Su San, Toshio Yamamoto
    • Journal Title

      Molecular Breeding

      Volume: 40 Issue: 2 Pages: 20-20

    • DOI

      10.1007/s11032-020-1101-5

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] Maintaining higher leaf photosynthesis after heading stage contributes to higher biomass accumulation in rice2021

    • Author(s)
      Sotaro Honda, Satoshi Ohkubo, Nan Su San, Anothai Nakkasame, Kazuki Tomisawa, Keisuke Katsura, Taiichiro Ookawa, Atsushi J. Nagano, Shunsuke Adachi
    • Organizer
      The 10th Asian Crop Science Association Conference
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 日本で育成されたイネ品種の光合成関連形質と育成年次との関係2020

    • Author(s)
      Nakkasame Anothai, 速水 健太郎, San Nan, 桂 圭佑, 松岡 信, 大川 泰一郎, 安達 俊輔
    • Organizer
      第250回日本作物学会講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] イネ個葉光合成速度の迅速多検体測定によるゲノムワイド関連解析2020

    • Author(s)
      関佳彬・速水健太郎・野村知宏・大川泰一郎・松岡信・田中佑・安達俊輔
    • Organizer
      日本作物学会関東支部会第109回講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 変動光条件の光合成速度に対するCO2濃度と施肥窒素量の相互作用2019

    • Author(s)
      大久保智司・田中佑・矢守航・安達俊輔
    • Organizer
      第10回日本光合成学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ドキュメントスキャナによるイネ葉身画像取得法の確立2019

    • Author(s)
      本田爽太郎・大久保智司・大川泰一郎・安達俊輔
    • Organizer
      日本作物学会関東支部第108回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] イネ止葉のCO2同化速度に関わるゲノムワイド関連解析2019

    • Author(s)
      速水健太郎・野村知宏・関佳彬・平野恒・松岡信・大川泰一郎・安達俊輔
    • Organizer
      第249回日本作物学会講演会
    • Related Report
      2019 Annual Research Report
  • [Remarks] 茨城大学研究者情報総覧

    • URL

      https://info.ibaraki.ac.jp/Profiles/108/0010720/profile.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] 茨城大学作物学研究室

    • URL

      http://cropscience.agr.ibaraki.ac.jp/wp/

    • Related Report
      2019 Annual Research Report

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Published: 2019-04-18   Modified: 2024-01-30  

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