• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Physiological mechanisms of carbohydrate utilization by N fixing endophyte for improving N fixation capacity in rice

Research Project

Project/Area Number 19H02941
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39020:Crop production science-related
Research InstitutionNagoya University

Principal Investigator

Kondo Motohiko  名古屋大学, 生命農学研究科, 教授 (00355538)

Co-Investigator(Kenkyū-buntansha) 杉浦 大輔  名古屋大学, 生命農学研究科, 講師 (50713913)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2021: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2020: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2019: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Keywordsイネ / 窒素固定 / エンドファイト / 炭水化物 / 品種
Outline of Research at the Start

茎部NSCと窒素固定の関係を詳細に解析するとともに菌の基質利用性、酸素要求性の解明により窒素固定活性の促進を図る。多様な品種に加えて近年開発されてきている、茎部のNSC蓄積を増大させた変異体や組換え体も活用しながら炭素基質の供給の増強により窒素固定能力が高まるかを検証するとともに、有効な菌叢を明らかにする。さらにイネーエンドファイト間での光合成産物の動態や有効な菌叢の特性の解析ならびに窒素固定活性を向上させるために有用な遺伝要因、栽培条件を明らかにすることでイネの窒素獲得能のポテンシャルの向上を試みる。

Outline of Final Research Achievements

We analyzed the physiological and genetic factors related to the variation and improvement of nitrogen-fixing activity by nitrogen-fixing endophytes in rice plants. We found that stem nitrogen fixation activity is affected by the accumulation of NSCs, especially free sugars, and that activity is high in African rice CG14 and other rice cultivars. The involvement of sugars was also verified by analyzing starch synthesis mutant lines and by using shading. Furthermore, we analyzed the flora in the stem of rice cultivars with different nitrogen-fixing ability, and found that α- and β-proteobacteria were the main nitrogen-fixing bacteria. The results of the evaluation of the effect of nitrogen fertilization on the nitrogen fixation capacity in the field showed that the contribution to the total nitrogen absorption was relatively small, but nitrogen fertilization did not have a clear effect on the cumulative nitrogen fixation capacity.

Academic Significance and Societal Importance of the Research Achievements

これまでほとんど知見のなかったイネ地上部のエンドファイト窒素固定の変動要因や関与する窒素固定菌叢の一端を示すことができた。活性の品種差異の要因のひとつが窒素固定に必要なエネルギーを供給する基質である可能性は、今後の活性の遺伝的向上のひとつの方向を示すものと考えている。イネは世界において主要作物であり、窒素化学肥料の削減を実現できればそのインパクトは大きいと考える。現状ではエンドファイトの寄与は非常に小さいが、今後土壌および体内エンドファイトを統合的に向上させる要因をさらに解明することが有効であると考える。

Report

(4 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (8 results)

All 2021 2020 2019

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Genotypic Variation of Endophytic Nitrogen-Fixing Activity and Bacterial Flora in Rice Stem Based on Sugar Content2021

    • Author(s)
      Okamoto Takanori、Shinjo Rina、Nishihara Arisa、Uesaka Kazuma、Tanaka Aiko、Sugiura Daisuke、Kondo Motohiko
    • Journal Title

      Frontiers in Plant Science

      Volume: 12 Pages: 719259-719259

    • DOI

      10.3389/fpls.2021.719259

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comprehensive analysis of the mechanisms underlying enhanced growth and root N acquisition in rice by the endophytic diazotroph, Burkholderia vietnamiensis RS12020

    • Author(s)
      Shinjo R, Tanaka A, Sugiura D, Suzuki T, Uesaka K, Takebayashi Y, Kojima M, Sakakibara H, Takemoto D, Kondo M
    • Journal Title

      Plant and Soil

      Volume: 450 Issue: 1-2 Pages: 537-555

    • DOI

      10.1007/s11104-020-04506-3

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] 連用圃場におけるイネの体内エンドファイト窒素固定能への窒素施肥の影響2021

    • Author(s)
      堀田幸奈・岡本卓哲・杉浦大輔・近藤始彦
    • Organizer
      021年度日本土壌肥料学会中部支部・中部土壌肥料研究会例会
    • Related Report
      2021 Annual Research Report
  • [Presentation] What Factor Affects Genotypic Difference in Endophytic Nitrogen-fixing Ability in Rice?2021

    • Author(s)
      Takanori Okamoto, Rina Shinjo, Arisa Nishihara,Kazuma Uesaka, Aiko Tanaka, Daisuke Sugiura,Motohiko Kondo
    • Organizer
      The 10th Asian Crop Science Association Conference
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 茎部の非構造性炭水化物蓄積および窒素固定細菌叢2021

    • Author(s)
      岡本卓哲,堀田幸奈,新庄莉奈,西原亜理沙,上坂一馬,田中愛子,杉浦大輔,近藤始彦
    • Organizer
      日本作物学会第251回講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 非構造性炭水化物蓄積量の異なるイネ品種における茎部窒素固定エンドファイトの菌叢解明2019

    • Author(s)
      岡本卓哲,新庄莉奈,西原亜理沙,上坂一馬,田中愛子,杉浦大輔,近藤始彦
    • Organizer
      日本作物学会第249回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Major microflora of endophytic nitrogen-fixing bacteria as Affected by non-structural carbohydrate in rice genotypes2019

    • Author(s)
      Takanori Okamoto, Rina Shinjo, Aiko Tanaka, Daisuke Sugiura, Motohiko Kondo
    • Organizer
      21st International Conference on Nitrogen Fixation,
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Can nitrogen fixing activity in rice stem be enhanced by high sugar concentration and low nitrogen fertilizer?2019

    • Author(s)
      Takanori Okamoto, Rina Shinjo, Aiko Tanaka, Daisuke Sugiura, Michelle Anne Bunquin, Olivyn Angeles, Pauline Chivenge and Motohiko Kondo
    • Organizer
      5th Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation.
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research

URL: 

Published: 2019-04-18   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi