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Study on the molecular mechanism of genotypic variation in the salt removal ability of rice leaf sheath

Research Project

Project/Area Number 19H02942
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39020:Crop production science-related
Research InstitutionNagoya University

Principal Investigator

MITSUYA Shiro  名古屋大学, 生命農学研究科, 准教授 (70432250)

Co-Investigator(Kenkyū-buntansha) カルタヘーナ ジョイス  名古屋大学, 生命農学研究科, 准教授 (10519929)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2021: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2019: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywordsイネ / 耐塩性 / 葉鞘 / 塩害 / 塩排除能 / 塩排除 / カリウム / 塩排出
Outline of Research at the Start

本研究の最終目的はイネの耐塩性を向上させることである。塩害下でイネは主に塩(NaCl)を吸収し、光合成を行う葉身に高濃度塩が蓄積すると光合成が阻害されて生育と収量が著しく減少する。そのため、イネの耐塩性には葉身の塩蓄積を増加させないため、塩排除能と呼ばれる機構が重要である。本研究ではイネの葉鞘が有する塩排除能に注目し、イネ葉鞘における塩排除能の向上の鍵となる遺伝子、分子機構を明らかにする。葉鞘における塩排除能に関わる遺伝子や品種間差を生じる分子機構が明らかになれば、葉鞘の塩排除能の活性を増加させる育種に繋がり、イネの耐塩性向上に繋がる。

Outline of Final Research Achievements

This study aimed to determine the molecular mechanism of salt removal ability in rice leaf sheath and to identify the causal gene and/or genetic loci. We used two methods, one is a genetic approach using genotypic variation, and another is a molecular approach using Tos17-inserted mutants. In the genetic approach, we used two rice varieties IR-44595 and 318 that showed differential ability regarding salt removal in leaf sheath. Using the F2 population between IR-44595 and 318, we successfully detected some QTL for Na removal ability. The QTL may contribute to improving rice salt tolerance by improving salt removal ability in leaf sheath. In addition, we found that OsNHX1 may be involved in the salt removal ability in leaf sheath by expressing in the peripheral vasculature.

Academic Significance and Societal Importance of the Research Achievements

本研究成果の学術的意義は、イネの耐塩性に重要な葉鞘の塩排除能の分子機構が解明された点である。イネの耐塩性は特に根の塩排除能により説明され、分子機構の解明も根の塩排除能に偏っていた。本研究では葉鞘の塩排除能の分子機構をさらに解明した点が重要である。
さらに社会的意義であるが、近年の気候変動により海水面が上昇し沿岸地域での塩害被害がより甚大になっている。アジアの沿岸地域では特に稲作が盛んであり塩害は解決すべき重要な課題である。本研究で見出した葉鞘の塩排除に係るQTLはイネの耐塩性を向上させることができるため、特に沿岸地域で見られる塩害によるイネ収量の減少を抑える方法を見出した点が社会的に意義がある。

Report

(4 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (24 results)

All 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (3 results) Journal Article (6 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 6 results,  Open Access: 4 results) Presentation (14 results) Book (1 results)

  • [Int'l Joint Research] 農林水産省(カンボジア)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] 国際イネ研究所(フィリピン)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] 国際イネ研究所(フィリピン)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] QTL mapping under salt stress in rice using a Kalarata-Azucena population.2022

    • Author(s)
      de Ocampo MP, Ho VT, Michael J. Thomson MJ, Mitsuya S, Yamauchi A, Ismail AM.
    • Journal Title

      Euphytica

      Volume: 218 Issue: 6 Pages: 74-74

    • DOI

      10.1007/s10681-022-03026-8

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Root sampling method for aquaporin gene expression analysis in rice2022

    • Author(s)
      Watanabe Y, Mitsuya S, Yamauchi A.
    • Journal Title

      Plant Root

      Volume: 16 Issue: 0 Pages: 11-20

    • DOI

      10.3117/plantroot.16.11

    • ISSN
      1881-6754
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] QTL analysis for sodium removal ability in rice leaf sheaths under salinity using an IR-44595/318 F2 population.2022

    • Author(s)
      Goto I, Neang S, Kuroki R, Reyes VP, Doi K, Skoulding NS, Taniguchi M, Yamauchi A, Mitsuya S.
    • Journal Title

      Frontiers in Plant Science

      Volume: 13 Pages: 1002605-1002605

    • DOI

      10.3389/fpls.2022.1002605

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Tissue-specific expression analysis of Na+ and Cl? transporter genes associated with salt removal ability in rice leaf sheath2020

    • Author(s)
      Neang Sarin、Goto Itsuki、Skoulding Nicola Stephanie、Cartagena Joyce A.、Kano-Nakata Mana、Yamauchi Akira、Mitsuya Shiro
    • Journal Title

      BMC Plant Biology

      Volume: 20 Issue: 1 Pages: 502-502

    • DOI

      10.1186/s12870-020-02718-4

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A GWAS approach to find SNPs associated with salt removal in rice leaf sheath2020

    • Author(s)
      Neang Sarin、de Ocampo Marjorie、Egdane James A、Platten John Damien、Ismail Abdelbagi M、Seki Masahide、Suzuki Yutaka、Skoulding Nicola Stephanie、Kano-Nakata Mana、Yamauchi Akira、Mitsuya Shiro
    • Journal Title

      Annals of Botany

      Volume: 126 Issue: 7 Pages: 1193-1202

    • DOI

      10.1093/aob/mcaa139

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Fundamental parenchyma cells are involved in Na+ and Cl- removal ability in rice leaf sheath2019

    • Author(s)
      5.Neang S., de Ocampo M., Egdane J.A., Platten J.D., Ismail A.M., Skoulding N.S., Kano-Nakata M., Yamauchi A., Mitsuya S.
    • Journal Title

      Functional Plant Biology

      Volume: 46 Issue: 8 Pages: 743-755

    • DOI

      10.1071/fp18318

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Evaluating salinity tolerance of rice varieties and soil salinity in coastal rice-cultivated areas of Cambodia. ミニシンポジウム:Challenges and Prospects of International Collaborative Studies in Asia (Coordinator: Mitsuya S).2023

    • Author(s)
      Neang S, Nget R, Kong K, Srun K, Kak B, Ken M, Heng S, Noeun S, Hong S, Yon S, Yamauchi A, Mitsuya S.
    • Organizer
      日本作物学会第255回講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] イネ葉鞘での塩応答遺伝子の網羅的解析とエピジェネティック制御の解明.2023

    • Author(s)
      浅原南, 谷口光隆, 三屋史朗.
    • Organizer
      日本作物学会第255回講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 乾燥ストレス後の再潅水がイネ器官間の代謝変動に与える影響.2022

    • Author(s)
      仲田(狩野)麻奈, 若山正隆, 門脇里恵, Deshabandu KHST, 山崎竜太朗, 三屋史朗, 山内章, 江原宏
    • Organizer
      日本作物学会第254回講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] イネの塩ストレス応答におけるアブシジン酸と過酸化水素蓄積の品種間差と耐塩性の関係.2022

    • Author(s)
      山本有美賀, 杉浦大輔, 山内章, 谷口光隆, 三屋史朗
    • Organizer
      日本作物学会第254回講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] OsAKT1遺伝子がイネ葉鞘でのカリウムイオン排除・蓄積に関わる.2022

    • Author(s)
      三宅拓郎, 谷口光隆, 山内章, 三屋史朗
    • Organizer
      日本作物学会第254回講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] イネに対する生育初期の一過的塩ストレスは生育中期の塩感受性を高める.2022

    • Author(s)
      鈴木海斗, 佐久間哲, 谷口光隆, 山内章, 三屋史朗
    • Organizer
      日本作物学会第254回講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Combining tolerance of salinity and submergence into IR64 background through marker-assisted backcrossing.2022

    • Author(s)
      De Ocampo MP, Egdane JA, Mitsuya S, Yamauchi A, Ismail AM.
    • Organizer
      日本作物学会第254回講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Attainable and actual yields, and yield-limiting constraints in irrigated rice growing areas in the Philippines.2022

    • Author(s)
      Perdiguerra KNC, Mitsuya S, Yamauchi A, Marajas IR, Cruz PCS.
    • Organizer
      日本作物学会第254回講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Analysis of molecular mechanisms of salt removal ability in leaf sheaths and the possibility of its application for molecular breeding in rice.2022

    • Author(s)
      Mitsuya S.
    • Organizer
      North Carolina Univ. - Nagoya Univ. Academic Consortium 21 Workshop.
    • Related Report
      2021 Annual Research Report
  • [Presentation] 葉鞘でのナトリウム排除能力の異なるイネ2品種におけるナトリウム輸送体の発現量解析2021

    • Author(s)
      後藤樹・山内章・三屋史朗
    • Organizer
      日本作物学会第251回講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 塩ストレストレーニングはストレス記憶により長期塩害条件で生育するイネの収量を向上する2021

    • Author(s)
      佐久間哲・山内章・三屋史朗
    • Organizer
      日本作物学会第251回講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 葉鞘の塩排除・隔離能の高いイネ品種の選抜と葉鞘内の塩分布2020

    • Author(s)
      後藤 樹, 山内 章, 三屋 史朗
    • Organizer
      日本作物学会第249回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Expression analysis of genes associated with the distribution of Na+ and Cl- in the internal leaf sheath tissues of rice2020

    • Author(s)
      Sarin Neang, Nicola Stephanie Skoulding, Mana Nakata-Kano, Akira Yamauchi and Shiro Mitsuya
    • Organizer
      日本作物学会第249回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Expression Analysis of Candidate Genes Associated with the Removal of Na+ and Cl- by Leaf Sheath in Rice2019

    • Author(s)
      Sarin Neang, Marjorie De Ocampo, James A. Egdane, John Damien Platten, Abdelbagi M. Ismail, Nicola Stephanie Skoulding, Mana Kano-Nakata, Akira Yamauchi, Shiro Mitsuya
    • Organizer
      日本作物学会第248回講演会
    • Related Report
      2019 Annual Research Report
  • [Book] 塩ストレスが植物に与える影響と適応機構(第1編 第2章 第6節, pp.77-87). 「バイオスティミュラントハンドブック:植物の生理活性プロセスから資材開発、適用事例まで(山内靖雄, 須藤修, 和田哲夫 監)」2022

    • Author(s)
      山根浩二, 大井崇生, 三屋史朗, 谷口光隆.
    • Total Pages
      460
    • Publisher
      エヌ・ティー・エス
    • Related Report
      2021 Annual Research Report

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Published: 2019-04-18   Modified: 2024-01-30  

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