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Genetic control of long term storage in melons

Research Project

Project/Area Number 19H02948
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39030:Horticultural science-related
Research InstitutionOkayama University

Principal Investigator

Ushijima Koichiro  岡山大学, 環境生命自然科学学域, 教授 (20379720)

Co-Investigator(Kenkyū-buntansha) 中野 龍平  京都大学, 農学研究科, 准教授 (70294444)
池田 和生  山形大学, 農学部, 准教授 (80555269)
門田 有希  岡山大学, 環境生命科学学域, 准教授 (30646089)
Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2023: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2021: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2019: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Keywordsメロン / エチレン / GWAS / 変異体 / エチレン合成 / 果実成熟 / 果肉軟化 / 成熟 / 果実 / エチレン生合成 / 果実軟化 / 棚持ち性
Outline of Research at the Start

果実として非常に人気の高いメロンであるが,日本人が好むメルティング質でジューシーな果肉の品種の棚持ち性は良いとは言いがたい.メロン品種は様々な変異に富んでおり,果肉軟化が非常に緩慢で棚持ち性の高い品種が存在する.本申請では,棚持ち性の良い品種を親とした分離集団を利用して,GWAS解析などの遺伝解析を通じて,原因遺伝子座の特定を行う.マーカー作出に加え,最終的には遺伝子組換えなどで原因遺伝子の特定に繋げたい.

Outline of Final Research Achievements

The melon is a juicy and popular fruit. Melons are climacteric fruits and their ripening is controlled by ethylene. This causes rapid ripening and poor shelf-life. This study was conducted to identify mutations in Honeydew and B2, melon varieties with good shelf-life. Segregated populations were created and genetic and GWAS analyses were conducted. Multiple loci were suggested to be involved for the defect in flesh softening trait in B2, but the loci could not be identified. For the defective ethylene synthesis trait in Honeydew, a single locus was predicted to be responsible, and GWAS analysis suggested the presence of the responsible gene on chromosome 8. There are no genes suggested to be involved in ethylene synthesis in this region, which may lead to the discovery of novel genes.

Academic Significance and Societal Importance of the Research Achievements

現在のクライマクテリック型果実の成熟機構に関する研究は成熟期におけるエチレン信号伝達そのものではなく,緑熟期における最初の自己触媒エチレンの合成を行う因子に焦点が移っている.しかし,成熟によって変化する形質は実際にはエチレン信号の下流で働く因子に制御されており,その因子を直接的に操作することが望ましい.本研究では下流で働く未知の因子の存在を示唆するものとなった.

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (4 results)

All 2023 2021 2020

All Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] メロンにおける改良 MIG-seq 利用の検討とエチレン合成関連遺伝子座の再評価2023

    • Author(s)
      影山 竜一,西村 和紗,中野 龍平,池田 和生,加藤 鎌司,久保 康隆,赤木 剛士,牛島 幸一郎
    • Organizer
      日本育種学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] メロンの成熟エチレン合成に関わる遺伝子座の同定とトランスクリプトーム解析2021

    • Author(s)
      小林和真・合田駿平・池田和生・赤木剛士・ 久保康隆・中野龍平・牛島幸一郎
    • Organizer
      園芸学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] メロン軟化遅延系統の遺伝的制御とトランスクリプトーム解析2021

    • Author(s)
      田駿平,池田和生,小林和真,赤木剛士,加藤鎌司,久保康孝,中野龍平,牛島幸一郎
    • Organizer
      園芸学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] The Genetic Control of Long Shelf Life in Melon (Cucumis melo L.)2020

    • Author(s)
      Shumpei Goda, Toshiki Hatanaka, Takashi Akagi, Yasutaka Kubo, Ryohei Nakano and Koichiro Ushijima
    • Organizer
      Plant and Animal Genome Conference XXVIII
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research

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Published: 2019-04-18   Modified: 2025-01-30  

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