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Geminivirus resistance in peppers and resistant breeding

Research Project

Project/Area Number 19H02950
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39030:Horticultural science-related
Research InstitutionKindai University

Principal Investigator

Koeda Sota  近畿大学, 農学部, 准教授 (00629066)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2022: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2021: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Keywordsトウガラシ・ピーマン / ジェミニウイルス / ベゴモウイルス / ウイルス抵抗性 / 抵抗性育種 / ゲノム解析 / ナス科 / 抵抗性遺伝子 / 野菜 / トウガラシ / 黄化葉巻病 / 抵抗性 / 育種 / 品種改良 / RADseq / Radseq / 全ゲノムリシーケンス / Rad-Seq / 耐病性
Outline of Research at the Start

ジェミニウイルスはタバココナジラミ媒介性のDNAウイルスであり,熱帯から温帯までの広範囲で園芸生産における脅威となっている.なかでも香辛料や野菜として世界的に需要の大きいトウガラシ属においては,生産現場で強く求められているジェミニウイルス耐病性品種が未だ報告されていない.本研究では,複数のジェミニウイルス耐病性トウガラシ系統を分子生物学的手法で解析し,交雑後代を用いたマッピングにより耐病性遺伝子の単離を試みる.本研究の成果が新たなジェミニウイルス耐病性品種の開発につながれば,トウガラシ生産における莫大な経済的損失が軽減できると期待される.

Outline of Final Research Achievements

Pepper yellow leaf curl disease (PepYLCD) is caused by viruses belonging to the Begomovirus genus of the Geminiviridae family. Over the past three decades, PepYLCD have caused substantial yield losses in peppers in many tropical and subtropical regions of the world. However, breeding of resistance to begomovirus is much less advanced in pepper than in tomato, and as of yet, there are no commercial pepper cultivars carrying resistance to begomovirus infection. In this study, genetic mapping of begomovirus resistance genes were conducted. Our study revealed that begomovirus resistance of GR1 (Capsicum chinense) is controlled by QTL. On the other hand, fine-mapping clarified that the recessive resistance gene pepy-1 encodes Pelota in BaPep-5 (Capsicum annuum) and the dominant resistance gene Pepy-2 encodes RNA dependent RNA polymerase (RDR) in PG1-1 (C. annuum). This is the first study to identify begomovirus resistance gene in peppers.

Academic Significance and Societal Importance of the Research Achievements

本研究では複数のベゴモウイルス抵抗性トウガラシを解析し,2つの抵抗性遺伝子を単離し,QTLの存在も明らかにした.これはトマト以外の植物種における初めての抵抗性遺伝子単離であり,学術的新規性がある.また,異なる抵抗性遺伝子を単離できたことから,抵抗性育種においても品種の多様化が可能であり,さらに複数の抵抗性遺伝子を有することで,より強い抵抗性を持つ品種を開発できる可能性が期待される.

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (50 results)

All 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (3 results) Journal Article (10 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 10 results,  Open Access: 3 results) Presentation (33 results) (of which Invited: 1 results) Remarks (4 results)

  • [Int'l Joint Research] 国立シアクアラ大学(インドネシア)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Syiah Kuala University(インドネシア)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Syiah Kuala University(インドネシア)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Pepper (<i>Capsicum annuum</i>) Plants Harboring the Begomovirus Resistance Gene <i>pepy-1</i> Show Delayed Symptom Progress and High Productivity Under Natural Field Conditions2023

    • Author(s)
      Pohan Nadya Syafira、Alfan Gian、Khalil Munawar、Bahagia Putra、Hayati Rayhan、Haidar Yusuf、Hadisah Nurul、Onouchi Mika、Shirono Ryosuke、Kohno Yoshinori、Hamada Ayane、Maruishi Tae、Hachisu Shinkai、Homma Kanami、Zakaria Sabaruddin、Kesumawati Elly、Koeda Sota
    • Journal Title

      The Horticulture Journal

      Volume: 92 Issue: 1 Pages: 36-46

    • DOI

      10.2503/hortj.QH-015

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identification of natural sources of resistance to bipartite begomovirus TYLCKaV in Solanum melongena2023

    • Author(s)
      Kikkawa Kyohei、Tanaka Minami、Kesumawati Elly、Koeda Sota
    • Journal Title

      Euphytica

      Volume: 219 Issue: 5 Pages: 51-51

    • DOI

      10.1007/s10681-023-03180-7

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification of QTLs conferring resistance to begomovirus isolate of PepYLCIV in Capsicum chinense2022

    • Author(s)
      Mori Namiko、Hasegawa Shota、Takimoto Ryota、Horiuchi Ryo、Watanabe Chiho、Onizaki Daiki、Shiragane Hayato、Nagano Atsushi J.、Kesumawati Elly、Koeda Sota
    • Journal Title

      Euphytica

      Volume: 218 Issue: 2 Pages: 20-20

    • DOI

      10.1007/s10681-022-02970-9

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] PepYLCIV and PepYLCAV resistance gene Pepy-2 encodes DFDGD-Class RNA-dependent RNA polymerase in Capsicum2022

    • Author(s)
      Koeda Sota、Mori Namiko、Horiuchi Ryo、Watanabe Chiho、Nagano Atsushi J.、Shiragane Hayato
    • Journal Title

      Theoretical and Applied Genetics

      Volume: 135 Issue: 7 Pages: 2437-2452

    • DOI

      10.1007/s00122-022-04125-9

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lisianthus enation leaf curl virus, a begomovirus new to Japan, is more virulent than the prevalent tomato yellow leaf curl virus in Ty-gene-mediated resistant tomato cultivars2022

    • Author(s)
      Taniguchi Marina、Sekine Ken-Taro、Koeda Sota
    • Journal Title

      Journal of General Plant Pathology

      Volume: 89 Issue: 1 Pages: 35-46

    • DOI

      10.1007/s10327-022-01101-5

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Southeast Asian Isolate of the Tomato Leaf Curl New Delhi Virus Shows Higher Pathogenicity Against Tomato and Cucurbit Crops Compared to that of the Mediterranean Isolate2021

    • Author(s)
      Yamamoto Hiroto、Wakita Yuya、Kitaoka Toshiya、Fujishiro Kohei、Kesumawati Elly、Koeda Sota
    • Journal Title

      The Horticulture Journal

      Volume: 90 Issue: 3 Pages: 314-325

    • DOI

      10.2503/hortj.UTD-269

    • NAID

      130008068026

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A recessive gene pepy-1 encoding Pelota confers resistance to begomovirus isolates of PepYLCIV and PepYLCAV in Capsicum annuum2021

    • Author(s)
      Koeda Sota、Onouchi Mika、Mori Namiko、Pohan Nadya Syafira、Nagano Atsushi J.、Kesumawati Elly
    • Journal Title

      Theoretical and Applied Genetics

      Volume: 134 Issue: 9 Pages: 2947-2964

    • DOI

      10.1007/s00122-021-03870-7

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Ty-2 and Ty-3a Conferred Resistance are Insufficient Against Tomato Yellow Leaf Curl Kanchanaburi Virus from Southeast Asia in Single or Mixed Infections of Tomato2020

    • Author(s)
      Koeda Sota、Fujiwara Ikuya、Oka Yuki、Kesumawati Elly、Zakaria Sabaruddin、Kanzaki Shinya
    • Journal Title

      Plant Disease

      Volume: 104 Issue: 12 Pages: 3221-3229

    • DOI

      10.1094/pdis-03-20-0613-re

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Pepper vein yellows virus 9: A novel polerovirus species isolated from chili pepper in Indonesia2020

    • Author(s)
      Sota Koeda, Kanami Homma, Mari Kamitani, Atsushi J. Nagano, Marina Taniguchi, Nadya Pohan and Elly Kesumawati
    • Journal Title

      Archives of Virology

      Volume: 165 Issue: 12 Pages: 3017-3021

    • DOI

      10.1007/s00705-020-04838-6

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular Characterization of Begomoviruses Associated with Yellow Leaf Curl Disease in Solanaceae and Cucurbitaceae Crops from Northern Sumatra, Indonesia2020

    • Author(s)
      Elly Kesumawati, Shoko Okabe, Munawar Khalil, Gian Alfan, Putra Bahagia, Nadya Pohan, Sabaruddin Zakaria and Sota Koeda
    • Journal Title

      Horticulture Journal

      Volume: In press

    • NAID

      130007879273

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ベゴモウイルスToLCNDVに対して抵抗性を示すメロン育種素材2023

    • Author(s)
      西川 拓・川原 武・中山実季・清水智貴・溝浦大輝・山本千尋・山本浩登・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Tomato leaf curl New Delhi virusに対して抵抗性を示す新規キュウリ素材2023

    • Author(s)
      中村有希・山本千尋・山本浩登・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] トマトにおけるTy-3aによるtomato yellow leaf curl virus抵抗性の高温による崩壊2023

    • Author(s)
      北脇新大・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Ty遺伝子の組み合わせがトマトのベゴモウイルス抵抗性に与える影響2023

    • Author(s)
      霜出萌乃・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Pelotaのウイルス誘導性ジーンサイレンシングはキュウリ(Cucumis sativus)にベゴモウイルス抵抗性を付与する2022

    • Author(s)
      小野内美佳・岡本桃花・森 涼馬・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Tomato leaf curl New Delhi virus(ToLCNDV)抵抗性を有する新たな育種素材におけるQTLの同定2022

    • Author(s)
      山本千尋・山本浩登・藤代康平・永野 惇・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] pepy-1によるベゴモウイルス抵抗性はトウガラシの高い果実生産性に寄与する2022

    • Author(s)
      Nadya Syafira Pohan・Rayhan Hayati・Nurul Hadisah・Yusuf Haidar・Munawar Khalil・小野内美佳・Elly Kesumawati・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Tomato yellow leaf curl Kanchanaburi virusに抵抗性を持つナス素材の接木接種による抵抗性評価2022

    • Author(s)
      吉川恭平・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 沖縄で単離されたlisianthus enation leaf curl virusはトマトのTy-2およびTy-3aによる抵抗性を回避する2022

    • Author(s)
      谷口満理奈・関根健太郎・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] トマトのTy-3aによる抵抗性を抑制するpepper yellow leaf curl Aceh virusの病原性因子2022

    • Author(s)
      Nadya Syafira Pohan・奥野佳苗・Elly Kesumawati・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Ty-3aを有するトマト抵抗性品種はLELCVとTYLCV-ILの複合感染により発病し,生育が阻害される2022

    • Author(s)
      谷口満理奈・関根健太郎・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] トマトTy抵抗性遺伝子のベゴモウイルスに対する有効性調査2022

    • Author(s)
      霜出萌乃・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] TYLCKaVはナス(Solanum melongena)に感染する主要なベゴモウイルスである2022

    • Author(s)
      吉川恭平・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] シロイヌナズナ(Arabidopsis thaliana)のPelota変異体はbeet curly top virus抵抗性を示す2022

    • Author(s)
      小野内美佳・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] キュウリ(Cucumis sativus)におけるToLCNDVの最適な機械的接種法2022

    • Author(s)
      山本千尋・中村有希・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] RDRをコードするPepy-2はトウガラシ(Capsicum annuum)にベゴモウイルス抵抗性を付与する2022

    • Author(s)
      小枝壮太・森 菜美子・堀内 亮・渡邉智帆・永野 惇 ・白銀隼人
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ナス科植物におけるウイルス性病害の防除に向けた研究2022

    • Author(s)
      小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Pelotaをコードする劣性遺伝子pepy-1はトウガラシ(Capsicum annuum)にベゴモウイルス抵抗性を付与する2021

    • Author(s)
      小野内美佳・森 菜美子・Nadya Syafira Pohan・永野 惇・Elly Kesumawati・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Capsicum chinenseにおけるベゴモウイルスPepYLCIVに対する抵抗性遺伝子のマッピング2021

    • Author(s)
      森 菜美子・長谷川翔太・瀧本涼太・堀内 亮・渡邉知帆・鬼﨑大樹・白銀隼人・永野 惇・Elly Kesumawati・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] キュウリにおけるtomato leaf curl New Delhi virus(ToLCNDV)抵抗性に関与するQTLの同定2021

    • Author(s)
      山本千尋・山本浩登・藤代康平・永野 惇・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] pepy-1を有するトウガラシBaPep-5は圃場においてベゴモウイルス耐性を示す2021

    • Author(s)
      Nadya Syafira Pohan・Gian Alfan・Munawar Khalil・Putra Bahagia・小野内美佳・城野良介・甲野喜識・濱田彩音・丸石多恵・蓮 真海・本間鹿波・Elly Kesumawati・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 沖縄県における園芸作物に感染するポレロウイルスおよびベゴモウイルスの野外調査2021

    • Author(s)
      谷口満理奈・関根健太郎・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 二分節型ベゴモウイルスであるTYLCKaVに対して抵抗性を示すナス(Solanum melongena)素材の選抜2021

    • Author(s)
      吉川恭平・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Pelotaのウイルス誘導性ジーンサイレンシングはキュウリ(Cucumis sativus)にベゴモウイルス抵抗性を付与する2021

    • Author(s)
      小野内美佳・岡本桃花・森 涼馬・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Tomato leaf curl New Delhi virus(ToLCNDV)抵抗性を有する新たな育種素材におけるQTLの同定2021

    • Author(s)
      山本千尋・山本浩登・藤代康平・永野 惇・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] pepy-1によるベゴモウイルス抵抗性はトウガラシの高い果実生産性に寄与する2021

    • Author(s)
      Nadya Syafira Pohan・Rayhan Hayati・Nurul Hadisah・Yusuf Haidar・Munawar Khalil・小野内美佳・Elly Kesumawati・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Tomato yellow leaf curl Kanchanaburi virusに抵抗性を持つナス素材の接木接種による抵抗性評価2021

    • Author(s)
      吉川恭平・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 沖縄で単離されたlisianthus enation leaf curl virusはトマトのTy-2およびTy-3aによる抵抗性を回避する2021

    • Author(s)
      谷口満理奈・関根健太郎・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ベゴモウイルスPepYLCIVに対して抵抗性を示すトウガラシ(Capsicum annuum)素材2021

    • Author(s)
      森 菜美子・堀内 亮・渡邉智帆・白銀隼人・Elly Kesumawati・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ベゴモウイルスToLCNDVに対して抵抗性を示すキュウリ育種素材2020

    • Author(s)
      山本浩登・藤代康平・白銀隼人・神崎真哉・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ベゴモウイルスPepYLCIVに対して抵抗性を示すCapsicum chinense育種素2020

    • Author(s)
      森 菜美子・鬼﨑大樹・Kesumawati Elly・Zakaria Sabaruddin・白銀隼人・神崎真哉・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] トウガラシにおける新大陸在来ベゴモウイルスの接種および耐病性評価系の構築2019

    • Author(s)
      森 菜美子・岡部祥子・Kesumawati Elly・Zakaria Sabaruddin・白銀隼人・神崎真哉・小枝壮太
    • Organizer
      園芸学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ベゴモウイルスPepYLCAVはナス科作物においてPepYLCIVよりも高い複製効率を示す2019

    • Author(s)
      岡部祥子・Kesumawati Elly・Zakaria Sabaruddin・神崎真哉・小枝壮太.
    • Organizer
      園芸学会
    • Related Report
      2019 Annual Research Report
  • [Remarks] 研究者ホームページ

    • URL

      https://sites.google.com/site/sotakoeda/home?authuser=0

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report 2020 Annual Research Report 2019 Annual Research Report
  • [Remarks] 論文のプレスリリース

    • URL

      https://newscast.jp/news/0263422

    • Related Report
      2022 Annual Research Report
  • [Remarks] 論文のプレスリリース

    • URL

      https://newscast.jp/news/9299674

    • Related Report
      2022 Annual Research Report
  • [Remarks] 論文のプレスリリース

    • URL

      https://newscast.jp/news/4254865

    • Related Report
      2022 Annual Research Report

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Published: 2019-04-18   Modified: 2024-01-30  

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