Regulation of virulence-related gene expression by Gas/Rsm-mediated quorum sensing in plant pathogenic bacteria

• Project/Area Number: 19H02956
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 39040:Plant protection science-related
• Research Institution: Okayama University
• Principal Investigator: Ichinose Yuki 岡山大学, 環境生命科学学域, 教授 (50213004)
• Co-Investigator(Kenkyū-buntansha): 松井 英譲 岡山大学, 環境生命科学学域, 准教授 (20598833)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2021: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2019: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: snRNA / rsmX / GacS/GacA二成分制御系 / 菌体密度感知機構 / ２成分制御系 / 非コードRNA / 翻訳制御 / 運動能 / Gac / Rsm / Small non-coding RNA / Gas / small non-coding RNA

Outline of Research at the Start

P. syringae pv. tomato DC3000 (PtoDC3000)は、菌体密度感知分子であるアシルホモセリンラクトン（AHL）を生産しない。本菌を高菌体密度で培養すると、タンパク質をコードしないsmall RNAであるrsmXやrsmYの発現が高まる。また、これらの発現はGacS/GacA２成分制御系の支配下にあることを明らかにしたが、AHLに代わる菌体密度感知機構は不明である。本研究では、PtoDC3000がAHL生産を廃止した理由を明らかにし、新規菌体密度感知分子の同定と、病原力関連遺伝子のrsmXやrsmYによる転写後発現制御機構を解明する。

Outline of Final Research Achievements

Pseudomonas syringae pv. tomato DC3000 (PtoDC3000) induces the expression of rsmX and rsmY at high cell density, which are small non-coding RNAs (snRNA) and is thought to control translation. The single snRAN deletion mutants we generated showed no remarkable difference from wild type (WT) strain. However, if we delete rsmX1, 2, 3, 4, 5 together, the mutant reduced the expression of flagella-related genes and type 3 secretion system (T3SS)-related genes. Furthermore, the mutant reduced the levels of swimming ability and virulence to host Arabidopsis thaliana. These results indicate that at low cell density RsmA (translation regulator) captures flagella- and T3SS-related mRNA and inhibit their expression, at high cell density newly synthesized rsmX RNA binds RsmA and activates these translation.

Academic Significance and Societal Importance of the Research Achievements

植物病原細菌には様々な病原性・病原力に関連した遺伝子が存在しているが、それらの遺伝子は常に発現しているのではなく、感染のステージに沿って必要な遺伝子を発現させる。本研究では病原力に関連する遺伝子発現がどのように制御されているかを解明しようとしたものであり、この分野の先端研究の一つである。本研究により非コード短鎖RNA分子が運動能や病原力に関係した遺伝子群の発現制御をしている可能性が示された。病原力の制御機構の解明は、その抑制機構への応用に道を開くものとして意義深い。

Research Products

• [Journal Article] Identification of Aerotaxis Receptor Proteins Involved in Host Plant Infection by <i>Pseudomonas syringae</i> pv. <i>tabaci</i> 6605 (2022)
  • Author(s): Tumewu, S. A., F., Matsui, H., Yamamoto, M., Noutoshi, Y., Toyoda, K., Ichinose, Y.
  • Journal Title: Microbes and Environments Volume: 37 Issue: 1 Pages: n/a
  • DOI: 10.1264/jsme2.ME21076
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access
• [Journal Article] HopAZ1, a type-III effector of Pseudomonas amygdali pv. tabaci, induces a hypersensitive response in tobacco wildfire-resistant Nicotiana tabacum cv. N509. (2022)
  • Author(s): Kashihara, S., Nishimura, T., Noutoshi, Y., Yamamoto, M., Toyoda, K., Ichinose, Y. and Matsui, H.
  • Journal Title: Molecular Plant Pathology Volume: 未定 Issue: 6 Pages: 885-894
  • DOI: 10.1111/mpp.13198
  • Peer Reviewed / Open Access
• [Journal Article] Complete Genome Sequence of Pseudomonas amygdali pv. tabaci Strain 6605, a Causal Agent of Tobacco Wildfire Disease (2021)
  • Author(s): Matsui Hidenori、Nishimura Takafumi、Asai Shuta、Masuda Sachiko、Shirasu Ken、Yamamoto Mikihiro、Noutoshi Yoshiteru、Toyoda Kazuhiro、Ichinose Yuki
  • Journal Title: Microbiology Resource Announcements Volume: 10 Issue: 28
  • DOI: 10.1128/mra.00405-21
  • Peer Reviewed / Open Access
• [Journal Article] Vfr targets promoter of genes encoding methyl-accepting chemotaxis protein in Pseudomonas syringae pv. tabaci 6605. (2021)
  • Author(s): Ogura, K., Matsui, H., Yamamoto, M., Noutoshi, Y., Toyoda, K., Taguchi, F. and Ichinose, Y.
  • Journal Title: Biochemistry and Biophysics Reports Volume: 26 Pages: 100944-100944
  • DOI: 10.1016/j.bbrep.2021.100944
  • Peer Reviewed / Open Access
• [Journal Article] Cluster II che genes of Pseudomonas syringae pv. tabaci 6605, orthologs of cluster I in Pseudomonas aeruginosa, are required for chemotaxis and virulence. (2021)
  • Author(s): Tumewu, S. A., Ogawa, Y., Okamoto, T., Sugihara, Y., Yamada, H., Taguchi, F., Matsui, H., Yamamoto, M., Noutoshi, Y., Toyoda, K. and Ichinose, Y.
  • Journal Title: Molecular Genetics and Genomics Volume: 296 Issue: 2 Pages: 299-312
  • DOI: 10.1007/s00438-020-01745-y
  • NAID: 120007182893
  • Peer Reviewed
• [Journal Article] Identification of chemoreceptor proteins for amino acids involved in host plant infection in Pseudomonas syringae pv. tabaci 6605. (2021)
  • Author(s): Tumewu, S. A., Matsui, H., Yamamoto, M., Noutoshi, Y., Toyoda, K., Ichinose, Y.
  • Journal Title: Microbiological Research Volume: 253 Pages: 126869-126869
  • DOI: 10.1016/j.micres.2021.126869
  • Peer Reviewed / Open Access
• [Journal Article] HopH1 effectors of Pseudomonas syringae pv. tomato DC3000 and pv. syringae B728a induce HR cell death in nonhost eggplant Solanum torvum (2020)
  • Author(s): Nahar Kamrun、Mukaihara Takafumi、Taguchi Fumiko、Matsui Hidenori、Yamamoto Mikihiro、Toyoda Kazuhiro、Noutoshi Yoshiteru、Shiraishi Tomonori、Ichinose Yuki
  • Journal Title: Journal of General Plant Pathology Volume: 87 Issue: 1 Pages: 24-29
  • DOI: 10.1007/s10327-020-00961-z
  • NAID: 120007163672
  • Peer Reviewed
• [Journal Article] PsyR, a transcriptional regulator in quorum sensing system, binds lux box-like sequence in psyI promoter without AHL quorum sensing molecule and activates psyI transcription with AHL in Pseudomonas syringae pv. tabaci 6605. (2020)
  • Author(s): Ichinose, Y., Tasaka, Y., Yamamoto, S., Inoue, Y., Takata, M., Nakatsu, Y., Taguchi, F., Yamamoto, M., Toyoda, K., Noutoshi, Y. and Matsui, H.
  • Journal Title: Journal of General Plant Pathology Volume: 86 Issue: 2 Pages: 124-133
  • DOI: 10.1007/s10327-019-00893-3
  • NAID: 120006900809
  • Peer Reviewed
• [Journal Article] Role of two sets of RND-type multidrug efflux pump transporter genes, mexAB-oprM and mexEF-oprN, in virulence of Pseudomonas syringae pv. tabaci 6605. (2020)
  • Author(s): Ichinose, Y., Nishimura, T., Harada, M., Kishiwagi, R., Yamamoto, M., Noutoshi, Y., Toyoda, K., Taguchi, F., Takemoto, D., Matsui, H.
  • Journal Title: Plant Pathology Journal Volume: 36 Issue: 2 Pages: 148-156
  • DOI: 10.5423/ppj.oa.11.2019.0273
  • Peer Reviewed / Open Access
• [Journal Article] Requirement of γ-Aminobutyric Acid Chemotaxis for Virulence of <i>Pseudomonas syringae</i> pv. <i>tabaci</i> 6605 (2020)
  • Author(s): Tumewu, S. A., Matsui, H., Yamamoto, M., Noutoshi, Y., Toyoda, K., Ichinose, Y.
  • Journal Title: Microbes and Environments Volume: 35 Issue: 4 Pages: n/a
  • DOI: 10.1264/jsme2.ME20114
  • NAID: 130007937288
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access
• [Presentation] タバコ野火病菌の Type III エフェクターが標的とする宿主因子の網羅的同定にむけて (2022)
  • Author(s): 黒江香那・樫原沙知・西村隆史・能年義輝・山本幹博・豊田和弘・中神弘史・一瀬勇規・松井英譲
  • Organizer: 令和４年度日本植物病理学会大会
• [Presentation] タバコ野火病菌の Type III effector HopAZ1の機能解析 (2022)
  • Author(s): 樫原沙知・西村隆史・能年義輝・山本幹博・豊田和弘・中神弘史・一瀬勇規・松井英譲
  • Organizer: 令和４年度日本植物病理学会大会
• [Presentation] Functional analysis of aerotaxis transducer genes in Pseudomonas syringae pv. tabaci 6605 (2021)
  • Author(s): Stephany Angelia Tumewu, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda and Yuki Ichinose
  • Organizer: 令和３年日本植物病理学会関西部会
• [Presentation] Pseudomonas syringae pv. tabaci 6605の走化性受容体遺伝子mcpの病原力への関与 (2021)
  • Author(s): 寺元 茜・金重 慎・Tumewu, S. A.・松井英譲・山本幹博・能年義輝・豊田和弘・一瀬勇規
  • Organizer: 令和3年度日本植物病理学会大会
• [Presentation] Identification of chemoreceptors for amino acids in Pseudomonas syringae pv. tabaci 6605 that involve in host plant infection (2021)
  • Author(s): Tumewu, S.A., Matsui, H., Yamamoto, M., Noutoshi, Y., Toyoda, K., and Ichinose, Y.
  • Organizer: 令和3年度日本植物病理学会大会
• [Presentation] The requirement of GABA chemotaxis for Pseudomonas syringae pv. tabaci 6605 virulence (2020)
  • Author(s): Tumewu, S. A.
  • Organizer: 12th Japan-US Seminar in Plant Pathology
  • Int'l Joint Research
• [Presentation] タバコ野火病菌の病徴進展に寄与するエフェクターの同定 (2020)
  • Author(s): 西村隆史・樫原沙知・能年義輝・山本幹博・豊田和弘・一瀬勇規・松井英譲
  • Organizer: 令和２年日本植物病理学会関西部会
• [Presentation] タバコ野火病抵抗性品種はタバコ野火病菌TTSSエフェクターを認識し，HRを誘導する (2020)
  • Author(s): 樫原沙知・西村隆史・能年義輝・山本幹博・豊田和弘・一瀬勇規・松井英譲
  • Organizer: 令和２年日本植物病理学会関西部会
• [Presentation] Requirement of GABA chemotaxis for virulence of Pseudomonas syringae pv. tabaci 6605 (2020)
  • Author(s): Stephany Angelia Tumewu, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda and Yuki Ichinose
  • Organizer: 令和２年日本植物病理学会関西部会
• [Presentation] The requirement of GABA chemotaxis for virulence of Pseudomonas syringae pv. tabaci 6605 (2020)
  • Author(s): Tumewu, S. A., Matsui, H., Yamamoto, M., Noutoshi, Y., Toyoda, K., and Ichinose, Y.
  • Organizer: 日本植物病理学会大会（大会は新型コロナウイルス 感染拡大防止のため中止となったが、発表は行われたこととして認められた）
• [Presentation] 植物病原性細菌のべん毛糖鎖を介した植物相互作用 (2019)
  • Author(s): 一瀬勇規
  • Organizer: 日本植物病理学会関西部会
  • Invited
• [Book] 植物病理学第２版（抗菌性物質分解酵素） (2020)
  • Author(s): 一瀬勇規
  • Total Pages: 347
  • Publisher: 文永堂
  • ISBN: 9784830041389
• [Book] 植物病理学第２版（細菌の病原性発現機構） (2020)
  • Author(s): 一瀬勇規・津下誠治
  • Total Pages: 347
  • Publisher: 文永堂
  • ISBN: 9784830041389
• [Book] 植物病理学第２版 (2020)
  • Author(s): 眞山滋志・土佐幸雄編（著者に一瀬勇規を含む）
  • Total Pages: 347
  • Publisher: 文永堂
  • ISBN: 9784830041389
• [Book] 植物たちの戦争 (2019)
  • Author(s): 日本植物病理学会編著（著者に一瀬勇規を含む）
  • Total Pages: 262
  • Publisher: 講談社
  • ISBN: 9784065152164