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Methodological developments of the connectivity analysis in marine benthos with an extremely dispersible planktonic larval stage

Research Project

Project/Area Number 19H03028
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40030:Aquatic bioproduction science-related
Research InstitutionThe University of Tokyo

Principal Investigator

Irie Takahiro  東京大学, 大気海洋研究所, 助教 (30549332)

Co-Investigator(Kenkyū-buntansha) 狩野 泰則  東京大学, 大気海洋研究所, 准教授 (20381056)
福森 啓晶  東北大学, 生命科学研究科, 助教 (60746569)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2022: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2021: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2020: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywords浮遊幼生 / 海産ベントス / 貝殻 / 石灰化 / 一塩基多型 / 種内変異 / 分散 / 海産無脊椎動物 / コネクティビティー / 元素比 / メタ個体群 / 次世代シーケンス / 幼生殻 / 微量元素比 / 酸素安定同位体比 / 温度 / EPMA / ICP-MS / 飼育実験 / カルシウム / ストロンチウム / WDS / 検量線 / 酸素安定同位体 / アラゴナイト / 腹足類
Outline of Research at the Start

水産生物の資源量を決める要因のひとつに、加入量があります。この研究では、その推定精度を高くするための技術を開発することを目指します。個体発生の初期に浮遊幼生期を持つ海産ベントスを対象に、インド-太平洋に広がる分布域の各地点からDNA試料を集め、最新の次世代シーケンス技術を用いて解析します。さらに、着底に成功した個体から得られた幼生殻の元素分析と海洋モデルの数値計算からの結果をベイズ統計学に基づくモデルに組み込むことで、移動率を高精度に推定するための枠組みを作ります。

Outline of Final Research Achievements

The goal of this project is to establish a technique for estimating the dispersal history of marine benthos during the planktonic larval stage, which is an important factor for understanding the recruitment dynamics from individual-derived information. The chemical composition of larval shells provides essential information for reconstructing the temperature history experienced by individuals prior to their settlement onto the benthic habitat. In this study, we succeeded in demonstrating that the elemental ratio (Sr/Ca), which can be analyzed at a lower cost than the oxygen stable isotope ratio, is significantly affected by intraspecific genotypic variation. We also established a calibration line to eliminate the influence of systematic errors in the determination of Sr/Ca in carbonate using EPMA. Furthermore, we proceeded to obtain nuclear genome information, which is necessary to estimate the connectivity among populations from the viewpoint of population genomics.

Academic Significance and Societal Importance of the Research Achievements

水産生物の加入量変動を決定する要因や原理の理解は、資源量評価や資源管理を進める上で、極めて重要な要素である。浮遊幼生期を持つ多くの水産生物では、個体発生初期の動態に関するデータを取得することが難しく、炭酸塩硬組織やゲノム情報の利用が必要不可欠である。また、生物起源の炭酸塩に含まれる元素比を左右する要因を定量的に理解することは、基礎科学における意義も大きい。炭酸塩中の元素比は、これまで熱力学的な過程が支配的であるというモデルで理解されてきた。今回、遺伝子型の個体差による影響が無視できない規模であることを実証できたことは、この分野でなされる今後の研究の方向性に一定の影響があるだろう。

Report

(5 results)
  • 2023 Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (14 results)

All 2024 2023 2022 2021 2020 2019

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (9 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results)

  • [Journal Article] Rearing in strontium-enriched water induces vaterite otoliths in the Japanese rice fish, Oryzias latipes2023

    • Author(s)
      Murase Iki、Kawamoto Tatsuhiko、Akizawa Norikatsu、Irie Takahiro
    • Journal Title

      Royal Society Open Science

      Volume: 10 Issue: 6

    • DOI

      10.1098/rsos.230410

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Population differentiation of the endangered salt-marsh snail <i>Ellobium chinense</i> in Japan (Gastropoda: Ellobiidae)2022

    • Author(s)
      Inoue Karin, Yahagi Takuya, Kimura Taeko, Kano Yasunori
    • Journal Title

      Plankton and Benthos Research

      Volume: 17 Issue: 1 Pages: 66-75

    • DOI

      10.3800/pbr.17.66

    • NAID

      130008163148

    • ISSN
      1880-8247, 1882-627X
    • Year and Date
      2022-02-22
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Negative temperature dependence of statolith Sr/Ca and its intraspecific variability in experimentally maintained spear squid <i>Heterololigo bleekeri</i>2022

    • Author(s)
      Shota Hosono, Takahiro Irie, Jun Yamamoto, Mitsuhiro Nakaya, Yasunori Sakurai, Tomohiko Kawamura and Yoko Iwata
    • Journal Title

      Journal of the Marine Biological Association UK

      Volume: 102 Issue: 5 Pages: 315-321

    • DOI

      10.1017/s0025315422000546

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Snails associated with the coral-killing sponge Terpios hoshinota in Okinawa Island, Japan.2021

    • Author(s)
      Yamashiro H, Fukumori H, Aini SN, Hirose Y.
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 20709-20709

    • DOI

      10.1038/s41598-021-00185-x

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] High temperature stress does not distort the geochemical thermometers based on biogenic calcium carbonate: Stable oxygen isotope values and Sr/Ca ratios of gastropod shells in response to rearing temperature2020

    • Author(s)
      Irie Takahiro、Suzuki Atsushi
    • Journal Title

      Geochimica et Cosmochimica Acta

      Volume: 288 Pages: 1-15

    • DOI

      10.1016/j.gca.2020.07.044

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 経験温度の復元を目的としたWDS検量線法の確立と腹足類幼生殻の元素比測定2024

    • Author(s)
      入江貴博・村瀬偉紀
    • Organizer
      日本生態学会第71回全国大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Statistical properties of X-ray intensity output for strontium in the analysis of aragonitic shells using EPMA with WDS.2023

    • Author(s)
      Iki Murase and Takahiro Irie
    • Organizer
      SEB Annual Conference 2023
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Multidimensional extension of the Fisher's principle explains the evolutionary stability of gender-role alternation in simultaneous hermaphrodites.2023

    • Author(s)
      Takahiro Irie and Seiji Arakaki
    • Organizer
      日本生態学会第70回全国大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Protandrous hermaphroditism with male regression: An explanation based on evolutionarily stable mixed strategies.2022

    • Author(s)
      Takahiro Irie and Seiji Arakaki
    • Organizer
      SEB Annual Conference 2022
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 炭酸塩酸素安定同位体比の温度依存性:気づいたこと・気をつけたいこと2022

    • Author(s)
      入江貴博
    • Organizer
      第17回バイオミネラリゼーションワークショップ
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Evaluation of statolith Sr/Ca as a thermometer by rearing experiments in spear squid <i>Heterololigo bleekeri</i>.2022

    • Author(s)
      Shota Hosono, Takahiro Irie, Jun Yamamoto, Mitsuhiro Nakaya, Yasunori Sakurai, Tomohiko Kawamura, Yoko Iwata
    • Organizer
      Cephalopod International Advisory Council 2022
    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Protandrous hermaphroditism with male regression: Is it evolutionarily stable or suboptimal exempt from natural selection?2022

    • Author(s)
      Takahiro IRIE and Seiji Arakaki
    • Organizer
      The 69th Annual Meeting of the Ecological Society of Japan
    • Related Report
      2020 Annual Research Report
  • [Presentation] Estimation of the variance component due to the temperature-size rule in wild populations of an intertidal gastropod with determinate growth.2019

    • Author(s)
      Takahiro Irie、Atsushi Suzuki
    • Organizer
      SEB 2019 Annual Meeting. Satellite Symposium: Is global warming causing animals to shrink? Evidence, Mechanisms and Models.
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dispersal footprint on water: Availability of Sr concentration of larval shells in widely distributed gastropods.2019

    • Author(s)
      Takahiro Irie
    • Organizer
      54th European Marine Biology Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research

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Published: 2019-04-18   Modified: 2025-01-30  

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