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Reproduction, predation and recruitment of fisheries resources evaluated by environmental DNA

Research Project

Project/Area Number 19H03031
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40030:Aquatic bioproduction science-related
Research InstitutionKyoto University

Principal Investigator

MASUDA Reiji  京都大学, フィールド科学教育研究センター, 教授 (60324662)

Co-Investigator(Kenkyū-buntansha) 源 利文  神戸大学, 人間発達環境学研究科, 教授 (50450656)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2022: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2021: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2020: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
KeywordsEnvironmental DNA / spawning / predation / recruitment / jack mackerel / Japanese anchovy / sea cucumber / black sea bream / 環境DNA / 被食 / 繁殖 / マナマコ / マアジ / 魚類 / 産卵 / 加入 / 沿岸加入 / 水産資源生物 / ニホンウナギ / クラゲ / クロダイ / 種特異的プライマー
Outline of Research at the Start

環境DNAは生物が水中に放出するDNAの総称であり,生物は平常時にも一定量を放出するとともに,繁殖行動に際しては,ミトコンドリアDNAに比べて多量の核DNAを放出する.また,被食個体は大量の体液を放出し,ここには長鎖のDNA断片が相対的に多いと予想される.そこで本研究では,主要な水産生物の飼育実験により,核DNAとミトコンドリアDNAの比で繁殖を,また長鎖と短鎖ミトコンドリアDNAの比で被食を,それぞれ検知できる可能性を探る.続いて沿岸から沖合までおよび河川の定点で採水し,環境DNA分析を行うとともに,卵や仔魚の採集も試み,環境DNA分析と従来の手法の結果を照合する.

Outline of Final Research Achievements

Environmental DNA technology for the quantification of marine species is developed targeting jack mackerel Trachurus japonicus, Japanese anchovy Engraulis japonicus, red-spotted grouper Epinephelus akaara, black sea bream Acanthopagrus shlegelii, and Japanese sea cucumber Apostichopus japonicus. Basic information such as effects of biomass, body size and interspecific mixing were also tested in tank experiments. High concentration of eDNA after the spawning was detected in jack mackerel and Japanese sea cucumber. Predation also induced eDNA peaks when Japanese anchovy, jack mackerel, and Japanese sea cucumber were preyed upon by their predators. Sampling in Tango Bay, off Kyoto, revealed that eDNA of black sea bream is detectable in 19 km offshore only in their spawning season (June) while it was detectable within 3 km offshore in other season. Environmental DNA is thus applicable to detect seasonal change of distribution in target species.

Academic Significance and Societal Importance of the Research Achievements

一連の水槽実験により、環境DNAを用いた水圏生物の定量が十分に可能であること、また環境DNAを用いた繁殖や被食の検出も可能である反面、これらは対象生物を定量する上でのノイズともなることが示された。またクロダイの分布を調べたフィールド調査でも、従来の曳網調査と矛盾しない結果が得られ、従来の調査よりも高い精度で行うことができた。環境DNAに基づく定量に関わる諸問題を解決し、海域のマクロ生物を対象とした調査で環境DNAを応用する道を拓いたことは、十分な学術的意義を持つ。一連の成果はまた、水産資源管理や生態系保全といった分野へ直接応用が可能であり、社会的な意義もすこぶる大きい。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (64 results)

All 2023 2022 2021 2020 2019 Other

All Journal Article (34 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 33 results,  Open Access: 23 results) Presentation (26 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Evaluation of community science monitoring with environmental DNA for marine fish species: “Fish survey project using environmental DNA”2023

    • Author(s)
      Suzuki‐Ohno Yukari、Tanabe Akifumi S.、Kasai Akihide、Masuda Reiji、Seino Satoquo、Dazai Akihiro、Suzuki Shota、Abe Takuzo、Kondoh Michio
    • Journal Title

      Environmental DNA

      Volume: NA Issue: 3 Pages: 613-623

    • DOI

      10.1002/edn3.425

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Estimating the spawning activity of fish species using nuclear and mitochondrial environmental DNA concentrations and their ratios.2023

    • Author(s)
      Wu, L., Wu, Q., Inagawa, T., Okitsu, J., Sakamoto, S., Minamoto, T.
    • Journal Title

      Freshwater Biology

      Volume: 68 Issue: 1 Pages: 103-114

    • DOI

      10.1111/fwb.14012

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An efficient early‐pooling protocol for environmental <scp>DNA</scp> metabarcoding2022

    • Author(s)
      Ushio Masayuki、Furukawa Saori、Murakami Hiroaki、Masuda Reiji、Nagano Atsushi J.
    • Journal Title

      Environmental DNA

      Volume: 4 Issue: 6 Pages: 1212-1228

    • DOI

      10.1002/edn3.337

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of the Persistence and Particle Size Distributional Shift of Sperm-Derived Environmental DNA to Monitor Jack Mackerel Spawning Activity2022

    • Author(s)
      Tsuji Satsuki、Murakami Hiroaki、Masuda Reiji
    • Journal Title

      Environmental Science &amp; Technology

      Volume: 56 Issue: 15 Pages: 10754-10763

    • DOI

      10.1021/acs.est.2c01904

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Environmental DNA analysis for macro-organisms: Species distribution and more.2022

    • Author(s)
      Minamoto, T.
    • Journal Title

      DNA Research

      Volume: 29

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spatiotemporal changes in environmental DNA concentrations caused by fish spawning activity.2022

    • Author(s)
      Wu, L., Yamamoto, Y., Yamaguchi, S., Minamoto, T.
    • Journal Title

      Ecological Indicators

      Volume: 142 Pages: 109213-109213

    • DOI

      10.1016/j.ecolind.2022.109213

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Seasonal changes in the distribution of black sea bream Acanthopagrus schlegelii estimated by environmental DNA2022

    • Author(s)
      Sasano Sachia、Murakami Hiroaki、Suzuki Keita W.、Minamoto Toshifumi、Yamashita Yoh、Masuda Reiji
    • Journal Title

      Fisheries Science

      Volume: 88 Issue: 1 Pages: 91-107

    • DOI

      10.1007/s12562-021-01572-z

    • NAID

      40022822024

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pre-centrifugation before DNA extraction mitigates extraction efficiency reduction of environmental DNA caused by the preservative solution (benzalkonium chloride) remaining in the filters2022

    • Author(s)
      Satsuki Tsuji, Ryohei Nakao, Minoru Saito, Toshifumi Minamoto ,Yoshihisa Akamatsu
    • Journal Title

      Limnology

      Volume: 23 Issue: 1 Pages: 9-16

    • DOI

      10.1007/s10201-021-00676-w

    • NAID

      40022800291

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Universal performance of benzalkonium chloride for the preservation of environmental <scp>DNA</scp> in seawater samples2021

    • Author(s)
      Jo Toshiaki、Sakata Masayuki K.、Murakami Hiroaki、Masuda Reiji、Minamoto Toshifumi
    • Journal Title

      Limnology and Oceanography: Methods

      Volume: 19 Issue: 11 Pages: 758-768

    • DOI

      10.1002/lom3.10459

    • NAID

      120007170799

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Utility of environmental DNA analysis for effective monitoring of invasive fish species in reservoirs.2021

    • Author(s)
      Jo T, Ikeda S, Fukuoka A, Inagawa T, Okitsu J, Katano I, Doi H, Nakai K, Ichiyanagi H, Minamoto T
    • Journal Title

      Ecosphere

      Volume: 12 Issue: 6 Pages: 0000-0000

    • DOI

      10.1002/ecs2.3643

    • NAID

      120007119350

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Complex interactions between environmental DNA (eDNA) state and water chemistries on eDNA persistence suggested by meta‐analyses2021

    • Author(s)
      Jo Toshiaki、Minamoto Toshifumi
    • Journal Title

      Molecular Ecology Resources

      Volume: 未定 Issue: 5 Pages: 1490-1503

    • DOI

      10.1111/1755-0998.13354

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Environmental DNA emission by two carangid fishes in single and mixed-species tanks2021

    • Author(s)
      Murakami Hiroaki、Masuda Reiji、Yamamoto Satoshi、Minamoto Toshifumi、Yamashita Yoh
    • Journal Title

      Fisheries Science

      Volume: 88 Issue: 1 Pages: 55-62

    • DOI

      10.1007/s12562-021-01565-y

    • NAID

      40022821990

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Environmental DNA preserved in marine sediment for detecting jellyfish blooms after a tsunami2021

    • Author(s)
      Ogata Mizuki、Masuda Reiji、Harino Hiroya、Sakata Masayuki K.、Hatakeyama Makoto、Yokoyama Katsuhide、Yamashita Yoh、Minamoto Toshifumi
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 16830-16830

    • DOI

      10.1038/s41598-021-94286-2

    • NAID

      120007141734

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular detection of giant snakeheads, Channa micropeltes (Cuvier, 1831), one of the most troublesome fish species.2021

    • Author(s)
      Osathanunkul M, Minamoto T
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 10 Pages: 9943-9943

    • DOI

      10.1111/ddi.13236

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intraspecific genetic variability and diurnal activity affect environmental DNA detection in Japanese eel2021

    • Author(s)
      Takahashi Sayaka、Takada Shingo、Yamanaka Hiroki、Masuda Reiji、Kasai Akihide
    • Journal Title

      PLOS ONE

      Volume: 16 Issue: 9 Pages: e0255576-e0255576

    • DOI

      10.1371/journal.pone.0255576

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Distribution of Japanese eel Anguilla japonica revealed by environmental DNA2021

    • Author(s)
      A. Kasai, A. Yamazaki, H. Ahn, H. Yamanaka, S. Kameyama, R. Masuda, N. Azuma, S. Kimura, Y. Yamashita
    • Journal Title

      Frontiers Ecology and Evolution

      Volume: 9 Pages: 1-11

    • DOI

      10.3389/fevo.2021.621461

    • NAID

      120006979765

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Simultaneous absolute quantification and sequencing of fish environmental DNA in a mesocosm by quantitative sequencing technique.2021

    • Author(s)
      Hoshino T, Nakao R, Doi H, Minamoto T
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 4372-4372

    • DOI

      10.1038/s41598-021-83318-6

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterizing the spatial and temporal occurrence patterns of the endangered botiid loach Parabotia curtus by environmental DNA analysis using a newly developed species-specific primer set.2021

    • Author(s)
      Sugiura K, Tomita S, Minamoto T, Mishina T, Iwata A, Abe T, Yamamoto S, Watanabe K
    • Journal Title

      Ichthyological Research

      Volume: 68 Issue: 1 Pages: 152-157

    • DOI

      10.1007/s10228-020-00756-4

    • NAID

      40022462123

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Settlement patterns of two sesarmid megalopae in the Sai River Estuary, Ishikawa Prefecture, Japan2020

    • Author(s)
      Matsumoto T, Arakawa H, Murakami T, Yanai S
    • Journal Title

      Plankton and Benthos Research

      Volume: 15 Issue: 4 Pages: 306-316

    • DOI

      10.3800/pbr.15.306

    • NAID

      130007938746

    • ISSN
      1880-8247, 1882-627X
    • Year and Date
      2020-11-18
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Determining an effective sampling method for eDNA metabarcoding: a case study for fish biodiversity monitoring in a small, natural river2020

    • Author(s)
      Sakata Masayuki K.、Watanabe Takeshi、Maki Nobutaka、Ikeda Kousuke、Kosuge Toshihiro、Okada Hiroaki、Yamanaka Hiroki、Sado Tetsuya、Miya Masaki、Minamoto Toshifumi
    • Journal Title

      Limnology

      Volume: 22 Issue: 2 Pages: 221-235

    • DOI

      10.1007/s10201-020-00645-9

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tropical fishes vanished after the operation of a nuclear power plant was suspended in the Sea of Japan2020

    • Author(s)
      Masuda Reiji
    • Journal Title

      PLOS ONE

      Volume: 15 Issue: 5 Pages: 0232065-0232065

    • DOI

      10.1371/journal.pone.0232065

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparing the efficiency of open and enclosed filtration systems in environmental DNA quantification for fish and jellyfish2020

    • Author(s)
      Takahashi Sayaka、Sakata Masayuki K.、Minamoto Toshifumi、Masuda Reiji
    • Journal Title

      PLOS ONE

      Volume: 15 Issue: 4 Pages: 0231718-0231718

    • DOI

      10.1371/journal.pone.0231718

    • NAID

      120006840121

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Selective collection of long fragments of environmental DNA using larger pore size filter2020

    • Author(s)
      Jo Toshiaki、Murakami Hiroaki、Masuda Reiji、Minamoto Toshifumi
    • Journal Title

      Science of The Total Environment

      Volume: 735 Pages: 139462-139462

    • DOI

      10.1016/j.scitotenv.2020.139462

    • NAID

      120006872998

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Estimating fish population abundance by integrating quantitative data on environmental DNA and hydrodynamic modelling2020

    • Author(s)
      Fukaya Keiichi、Murakami Hiroaki、Yoon Seokjin、Minami Kenji、Osada Yutaka、Yamamoto Satoshi、Masuda Reiji、Kasai Akihide、Miyashita Kazushi、Minamoto Toshifumi、Kondoh Michio
    • Journal Title

      Molecular Ecology

      Volume: NA Issue: 13 Pages: 3057-3067

    • DOI

      10.1111/mec.15530

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A molecular survey based on eDNA to assess the presence of a clown featherback (Chitala ornata) in a confined environment.2020

    • Author(s)
      Osathanunkul, M, Minamoto T
    • Journal Title

      PeerJ,

      Volume: 8 Pages: e10338-e10338

    • DOI

      10.7717/peerj.10338

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sedimentary DNA tracks decadal-centennial changes in fish abundance2020

    • Author(s)
      Kuwae Michinobu、Tamai Hiromichi、Doi Hideyuki、Sakata Masayuki K.、Minamoto Toshifumi、Suzuki Yoshiaki
    • Journal Title

      Communications Biology

      Volume: 3 Issue: 1 Pages: 1-12

    • DOI

      10.1038/s42003-020-01282-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of sampling season and location in fish environmental DNA metabarcording in dam reservoirs.2020

    • Author(s)
      Hayami K, Sakata MK, Inagawa T, Okitsu J, Katano I, Nakai K, Ichiyanagi H, Gotoh RO, Miya M, Sato H, Yamanaka H, Minamoto T.
    • Journal Title

      Ecology and Evolutions

      Volume: 10 Issue: 12 Pages: 5354-5367

    • DOI

      10.1002/ece3.6279

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] An illustrated manual for environmental DNA research: Water sampling guidelines and experimental protocols2020

    • Author(s)
      Minamoto Toshifumi、Miya Masaki、Sado Tetsuya、Seino Satoquo、Doi Hideyuki、Kondoh Michio、Nakamura Keigo、Takahara Teruhiko、Yamamoto Satoshi、Yamanaka Hiroki、Araki Hitoshi、Iwasaki Wataru、Kasai Akihide、Masuda Reiji、Uchii Kimiko
    • Journal Title

      Environmental DNA

      Volume: 3 Issue: 1 Pages: 8-13

    • DOI

      10.1002/edn3.121

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 海洋における環境DNA研究の現状と今後の展望.2020

    • Author(s)
      村上弘章・益田玲爾
    • Journal Title

      沿岸域学会誌

      Volume: 33 Pages: 44-56

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Limited adaptation to non-natal osmotic environments at high water temperature in euryhaline wanderer fishes.2020

    • Author(s)
      Nakamura M, Watanabe S, Kaneko T, Masuda R, Tsukamoto K, Otake T
    • Journal Title

      Environmental Biology of Fishes

      Volume: 103 Issue: 2 Pages: 137-145

    • DOI

      10.1007/s10641-019-00940-0

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biomass-dependent emission of environmental DNA in jack mackerel Trachurus japonicus juveniles.2019

    • Author(s)
      Horiuchi T, Masuda R*, Murakami H, Yamamoto S, Minamoto T
    • Journal Title

      Journal of Fish Biology

      Volume: 95 Issue: 3 Pages: 979-981

    • DOI

      10.1111/jfb.14095

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Particle size distribution of environmental DNA from the nuclei of marine fish.2019

    • Author(s)
      Jo T, Arimoto M, Murakami H, Masuda R, Minamoto T
    • Journal Title

      Environmental Science & Technology

      Volume: 53 Issue: 16 Pages: 9947-9956

    • DOI

      10.1021/acs.est.9b02833

    • NAID

      120007002586

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Seasonal and interannual variation in the density of visible Apostichopus japonicus (Japanese sea cucumber) in relation to sea water temperature.2019

    • Author(s)
      Minami K, Masuda R, Takahashi K, Sawada H, Shirakawa H, Yamashita Y.
    • Journal Title

      Estuarine, Coastal and Shelf Science

      Volume: 229 Pages: 106384-106384

    • DOI

      10.1016/j.ecss.2019.106384

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Estimating shedding and decay rates of environmental nuclear DNA with relation to water temperature and biomass2019

    • Author(s)
      Jo T, Murakami H, Masuda R, Minamoto T
    • Journal Title

      Environmental DNA

      Volume: 2 (2) Issue: 2 Pages: 140-151

    • DOI

      10.1002/edn3.51

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 環境DNA分析を用いた舞鶴湾におけるマアジ地先産卵の時期および場所の推定.2023

    • Author(s)
      辻冴月・村上弘章・益田玲爾
    • Organizer
      日本生態学会第70回全国大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 環境DNAから見たクロダイの河川進入の季節性と地域性.2023

    • Author(s)
      笹野祥愛・村上弘章・河合賢太郎・海野徹也・山下洋・益田玲爾
    • Organizer
      日本水産学会春季大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 潜水で見た舞鶴湾における魚類群集の過去21年間の変遷.2023

    • Author(s)
      益田玲爾
    • Organizer
      日本水産学会春季大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Temperature sensitivity of the interspecific interaction strength of coastal marine fish communities.2023

    • Author(s)
      Ushio M, Sado T, Fukuchi T, Sasano S, Masuda R, Osada Y, Miya M
    • Organizer
      The eDNA Society International Meeting 2023.
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The combinational analyses of environmental and stomach content DNA may reveal piscivory of the Japanese Spiny Lobster (Panulirus japonicus).2023

    • Author(s)
      Shi S, Masuda R
    • Organizer
      The eDNA Society International Meeting 2023.
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Horizontal and vertical distribution of environmental DNA of jack mackerel Trachurus japonicus and Japanese anchovy Engraulis japonicus in Maizuru Bay, Kyoto Prefecture.2023

    • Author(s)
      Murakami H, Fukaya K, Yamamoto S, Minamoto T, Minami K, Miyashita K, Kameoka T, Masuda R, Yamashita Y, Kondoh M.
    • Organizer
      The eDNA Society International Meeting 2023.
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Ocean as Core Biome.2022

    • Author(s)
      Masuda R.
    • Organizer
      日本地球惑星科学連合2022年大会
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 環境DNAメタバーコーディング解析で読み解く京都府由良川の魚類相の季節変化.2022

    • Author(s)
      村上弘章・笹野祥愛・益田玲爾・山下洋・笠井亮秀
    • Organizer
      環境DNA学会オンラインワークショップ
    • Related Report
      2022 Annual Research Report
  • [Presentation] 環境DNAで読み解く京都府由良川のスズキLateolabrax japonicusの分布と季節回遊.2021

    • Author(s)
      村上弘章・笹野祥愛・益田玲爾・笠井亮秀・山下洋
    • Organizer
      環境DNA学会第4回大会.北海道大学,札幌(オンライン).
    • Related Report
      2021 Annual Research Report
  • [Presentation] 産卵と被食に伴う核およびミトコンドリア環境DNAの動態:カタクチイワシとマアジを用いた水槽実験.2021

    • Author(s)
      上村真太郎・益田玲爾・村上弘章・徐寿明・源利文.
    • Organizer
      日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 環境DNA分析による全国の河川におけるニホンウナギ分布域の推定.2021

    • Author(s)
      笠井亮秀・山崎彩・安孝珍・山中裕樹・亀山哲・益田玲爾・東信行・木村伸吾・唐木達郎・黒川優子・山下洋.
    • Organizer
      日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 環境DNAで読み解く河川と沿岸域の魚類多様性とその生態.2021

    • Author(s)
      村上弘章・内藤隆慈・志水正敏・益田玲爾・笠井亮秀.
    • Organizer
      日本生態学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 長鎖・核DNAおよび海水サンプルを対象とした塩化ベンザルコニウムの環境DNA保存効果.2021

    • Author(s)
      徐寿明・村上弘章・益田玲爾・源利文.
    • Organizer
      日本生態学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 堆積物中における環境DNAの残存性とこれを応用した津波後のクラゲ類ブルームの検出.環2020

    • Author(s)
      尾形瑞紀・*益田玲爾・張野宏也・坂田雅之・畠山信・横山勝英・山下洋・源利文.
    • Organizer
      環境DNA学会第3回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 環境DNA分析によるマナマコ(Apostichopus japonicus)の繁殖期推定.2020

    • Author(s)
      竹下大輝・村上弘章・益田玲爾・源利文.
    • Organizer
      環境DNA学会第3回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 異種混合飼育が海産魚の環境DNA放出量に与える影響.2020

    • Author(s)
      村上弘章・益田玲爾・山本哲史・源利文・山下洋.
    • Organizer
      環境DNA学会第3回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ニホンウナギの環境DNA放出量に与える遺伝子変異と活動日周性の影響.2020

    • Author(s)
      高橋さやか・高田真吾・山中裕樹・益田玲爾・笠井亮秀.
    • Organizer
      環境DNA学会第3回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] キジハタの環境DNAに対する体サイズおよび活動量の影響.2020

    • Author(s)
      曽根高幹広・益田玲爾・徐寿明・竹下大輝・村上弘章・上村真太郎・源利文.
    • Organizer
      環境DNA学会第3回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 粗孔径フィルターを利用したマアジ長鎖環境DNAの効率的回収.2020

    • Author(s)
      徐寿明・村上弘章・益田玲爾・源利文
    • Organizer
      令和2年度日本水産学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] マナマコ浮遊幼生出現数からみた産卵と月周期の関係.2019

    • Author(s)
      澤田英樹・高橋宏司・益田玲爾・山下洋
    • Organizer
      2019年度プランクトンベントス学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Biomass-dependent emission of environmental DNA in jack mackerel Trachurus japonicus juveniles.2019

    • Author(s)
      Horiuchi T, *Masuda R, Murakami H, Yamamoto S, Minamoto T.
    • Organizer
      Fisheries Society of the British Isles Symposium 2019.
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Distributional pattern of black sea bream Acanthopagrus schlegelii estimated from environmental DNA: seasonal changes in marine and river waters.2019

    • Author(s)
      Sasano S, Murakami H, Suzuki KW, Minamoto T, Yamashita Y, Masuda R.
    • Organizer
      Fisheries Society of the British Isles Symposium 2019.
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マアジの核およびミトコンドリアに由来する環境DNAの水温・体サイズ依存.2019

    • Author(s)
      徐寿明・有本美於・村上弘章・益田玲爾・源利文.
    • Organizer
      令和元年度日本水産学会秋季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 舞鶴湾におけるマアジ環境DNA濃度の日周変化.2019

    • Author(s)
      村上弘章・益田玲爾・山下洋
    • Organizer
      第2回環境DNA学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] クロダイ受精卵および仔稚魚の個体あたり環境DNA放出量.2019

    • Author(s)
      笹野祥愛・益田玲爾・山下洋
    • Organizer
      第2回環境DNA学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 魚類の核およびミトコンドリアに由来する環境DNAの粒子径サイズ分布.2019

    • Author(s)
      徐寿明・有本美於・村上弘章・益田玲爾・源利文
    • Organizer
      2019年度日本分子生物学会大会
    • Related Report
      2019 Annual Research Report
  • [Book] 里海フィールド科学 京都の海に学ぶ人と自然のつながり2022

    • Author(s)
      山下洋・益田玲爾・甲斐嘉晃・鈴木啓太・高橋宏司・邉見由美編
    • Total Pages
      385
    • Publisher
      京都大学学術出版会
    • ISBN
      9784814004454
    • Related Report
      2022 Annual Research Report
  • [Book] 第6章 環境DNAを用いた大規模生態系観測.土居秀幸・近藤倫生編『環境DNA 生態系の真の姿を読み解く』2021

    • Author(s)
      近藤倫生・清野聡子・益田玲爾・笠井亮秀(2021)
    • Total Pages
      291
    • Publisher
      共立出版
    • ISBN
      9784320058163
    • Related Report
      2021 Annual Research Report
  • [Remarks] 堆積物の環境DNAで探る過去の出来事 -津波直後のクラゲ大発生を検知-

    • URL

      https://www.kyoto-u.ac.jp/ja/research-news/2021-08-23-0

    • Related Report
      2021 Annual Research Report
  • [Remarks] 環境DNA分析による個体数の推定法を実証 -汲んだ水から魚を数える-

    • URL

      https://www.kyoto-u.ac.jp/ja/research-news/2020-07-07-2

    • Related Report
      2021 Annual Research Report

URL: 

Published: 2019-04-18   Modified: 2024-01-30  

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