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Dissecting molecular mechanisms of gonadal sexual plasticity by combining experimental fish models with single-cell analysis

Research Project

Project/Area Number 19H03049
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionKyushu University

Principal Investigator

Ohta Kohei  九州大学, 農学研究院, 教授 (10585764)

Co-Investigator(Kenkyū-buntansha) 荻野 由紀子  九州大学, 農学研究院, 准教授 (00404343)
Chakraborty Tapas (C Tapas)  九州大学, 農学研究院, 助教 (70715440)
Mohapatra Sipra  九州大学, 農学研究院, 学術研究員 (80715441)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2021: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2020: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2019: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Keywords性的可塑性 / 生殖幹細胞 / 魚類 / 性転換 / シングルセル解析 / シングルセル / エピジェネティクス / q
Outline of Research at the Start

魚類の性を自在に統御するには、それぞれの種が有する性的可塑性の特徴を細胞レベルで理解する必要がある。本研究では、研究代表者らは、自らが構築した性転換魚のモデル系を用い、生殖腺の性転換の起点となる2種の細胞集団(“生殖幹細胞”と“未分化体細胞”)に焦点を絞り、シングルセル解析系を導入して 生殖腺の転換の仕組みを解析する。さらに、雌雄異体魚との比較を行い、将来、全ての魚類において性を自在に統御するために必要な“細胞の共通原理”に関する情報を得る。

Outline of Final Research Achievements

To understand the molecular and cellular mechanisms of gonadal sexual plasticity, we investigated the germ line stem cells (GSCs) and undifferentiated somatic cells (SSCs) in the gonad of a sex-changing fish. These GSCs and SSCs have been suggested to be the origin of testicular tissue during sex change from female to male. In this study, we performed single-cell RNA-seq analysis of GSCs and SSCs. As a result, we revealed the heterogeneity of GSCs and SSCs, respectively, and clarified the change in their gene expression patterns during gonadal sex change. More interestingly, we also found that SSCs switch the pattern of differentiation between ovarian somatic cells and testicular somatic cells. On the other hand, using gonochoristic fish, the involvement of oct4 gene in the maintenance of GSCs was demonstrated. Moreover, the result of in vitro culture experiment suggests the differences in sexual plasticity of gonadal somatic cells among fish species.

Academic Significance and Societal Importance of the Research Achievements

性転換魚のモデル系などを用いて魚類生殖腺の性的可塑性のメカニズムを解析した結果、卵巣から精巣への転換に伴う生殖幹細胞の連続的な遺伝子発現変化を明らかにするとともに、未分化体細胞が卵巣型から精巣型へと分化パターンを切りかえることを初めて見出した。また、生殖幹細胞で発現するoct4遺伝子が幹細胞性の維持に関与すること、魚種間で生殖腺体細胞の性的可塑性に違いがあることなどを解明した。今後、これらの成果をもとに、生殖腺全体や個体での性を統御する機構へと理解を深めることが可能となり、脊椎動物一般の性的可塑性の分子・細胞機構の研究に貢献するとともに、水産における新たな性統御法の開発にも繋がると期待される。

Report

(4 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (26 results)

All 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (3 results) Journal Article (6 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 6 results,  Open Access: 4 results) Presentation (15 results) (of which Int'l Joint Research: 8 results) Book (1 results) Remarks (1 results)

  • [Int'l Joint Research] Southwest University(中国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Southwest University(中国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Southwest University(中国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Evolutionary differentiation of androgen receptor is responsible for sexual characteristic development in a teleost fish2023

    • Author(s)
      Y. Ogino, S. Ansai, E. Watanabe, M. Yasugi, Y. Katayama, H. Sakamoto, K. Okamoto, K. Okubo, Y. Yamamoto, I. Hara, T. Yamazaki, A. Kato, Y. Kamei, K. Naruse, K. Ohta, H. Ogino, T. Sakamoto, S. Miyagawa, T. Sato, G. Yamada, M. Baker & T. Iguchi
    • Journal Title

      Nature communications

      Volume: 14 Issue: 1 Pages: 1428-1428

    • DOI

      10.1038/s41467-023-37026-6

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Divulging the social sex change mechanism in a unique model system for studying the sexual plasticity of protogynous hermaphrodite fish, three bamboo leaf wrasse (Pseudolabrus sieboldi)2022

    • Author(s)
      Chakraborty Tapas、Yamamoto Yume、Hanai Shoma、Hirano Mayumi、Mohapatra Sipra、Yamaguchi Akihiko、Takeda Tatsusuke、Matsuyama Michiya、Ohta Kohei
    • Journal Title

      Frontiers in Marine Science

      Volume: 9 Pages: 1048506-1048506

    • DOI

      10.3389/fmars.2022.1048506

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sex Lethal Gene Manipulates Gonadal Development of Medaka, Oryzias latipes, through Estrogenic Interventions2022

    • Author(s)
      Chakraborty Tapas、Mohapatra Sipra、Matsuyama Michiya、Nagahama Yoshitaka、Ohta Kohei
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 23 Issue: 24 Pages: 15496-15496

    • DOI

      10.3390/ijms232415496

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sex determination, gonadal sex differentiation, and plasticity in vertebrate species2021

    • Author(s)
      Nagahama Yoshitaka、Chakraborty Tapas、Paul-Prasanth Bindhu、Ohta Kohei、Nakamura Masaru
    • Journal Title

      Physiological Reviews

      Volume: 101 Issue: 3 Pages: 1237-1308

    • DOI

      10.1152/physrev.00044.2019

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Estrogen and estrogen receptors chauffeur the sex-biased autophagic action in liver2020

    • Author(s)
      Sipra Mohapatra, Tapas Chakraborty, Sonoko Shimizu, Kayoko Ohta, Yoshitaka Nagahama, Kohei Ohta
    • Journal Title

      Cell Death & Differentiation

      Volume: NA Issue: 11 Pages: 3117-3130

    • DOI

      10.1038/s41418-020-0567-3

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Estrogen receptor β2 oversees germ cell maintenance and gonadal sex differentiation in medaka, Oryzias latipes2019

    • Author(s)
      2.Tapas Chakraborty, Sipra Mohapatra, Lin yan Zhou, Takahiro Matsubara, Taisen Iguchi, Yoshitaka Nagahama
    • Journal Title

      Stem Cell Reports

      Volume: 13 Issue: 2 Pages: 419-433

    • DOI

      10.1016/j.stemcr.2019.07.013

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Gonadal stem cell: a possible solution of futuristic aquaculture2023

    • Author(s)
      Tapas Chakraborty, Sota Kawao, Kodai mizumura, Akira Eguchi, Sipra Mohapatra, Takehiko Itoh, Atsushi Toyoda, Naoki Nagano, Kohei Ohta, Michiya Matsuyama
    • Organizer
      令和5年度日本水産学会春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Involvement of the pituitary gland in social-environment-dependent sex change2022

    • Author(s)
      Yume Yamamoto, Shoma Hanai, Tapas Chakraborty, Astushi Toyoda, Takehiko Itoh, Michiya Matsuyama, Yoshitaka Nagahama, Kohei Ohta
    • Organizer
      International Conference on Responsible Aquaculture and Sustainable Fisheries Interact
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gonadal stem cells in a sex changing fish, Pseudolabrus sieboldi2022

    • Author(s)
      Kodai Mizumura, Tapas Chakraborty, Kazuki Tateyama, Shoma Hanai, Yoshihara Yuya, Yume Yamamoto, Sipra Mohapatra, Yumiko Yamasaki, Atsushi Toyota, Takehiko Itoh, Michiya Matsuyama, Yoshitaka Nagahama, Kohei Ohta
    • Organizer
      The International Symposium “Totipotency and Germ cell Development”
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 海産魚における生殖幹細胞の長期培養法2022

    • Author(s)
      水村航大, Chakraborty Tapas, 八尋逸清, 太田耕平, 長濱嘉孝, 松山倫也
    • Organizer
      令和4年度日本水産学会秋季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 社会環境依存型の性転換における脳下垂体の関与2022

    • Author(s)
      山本優芽, 花井匠磨, Chakraborty Tapas, 豊田敦, 伊藤武彦, 松山倫也, 長濱嘉孝, 太田耕平
    • Organizer
      令和4年度日本水産学会秋季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Molecular mechanism of sexual plasticity in fish: a viewpoint from a natural sex changing fish2022

    • Author(s)
      Kohei Ohta, Tapas Chakraborty, Kazuki Tateyama, Shoma Hanai, Kodai Mizumura, Yume Yamamoto, Yuya Yoshihara, Sipra Mohapatra, Michiya Matsuyama, Yoshitaka Nagahama
    • Organizer
      Joint Conference of the 30th European Society for Comparative Endocrinology and the 9th International Society for Fish Endocrinology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Oct4 and sexual plasticity in fish gonad2022

    • Author(s)
      Tapas Chakraborty, Sipra Mohapatra, Kohei Ohta, Michiya Matsuyama, Yoshitaka Nagahama
    • Organizer
      Joint Conference of the 30th European Society for Comparative Endocrinology and the 9th International Society for Fish Endocrinology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Autophagy: an important player in germ cell maintenance and gonadal sexuality2022

    • Author(s)
      Sipra Mohapatra, Tapas Chakraborty, Naoki Nagano, Yoshitaka Nagahama, Kohei Ohta, Michiya Matsuyama
    • Organizer
      Joint Conference of the 30th European Society for Comparative Endocrinology and the 9th International Society for Fish Endocrinology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] カタクチイワシ Engraulis japonicus をモデルとした生殖腺の性差に関わるエピジェネティック修飾の解析法2022

    • Author(s)
      江藤悠平・Tapas Chakraborty・北野 載・ 豊田 敦・伊藤武彦・ 松山倫也・太田耕平
    • Organizer
      令和4年度日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ホシササノハベラ生殖腺の性転換に伴う細胞増殖動態と血中ステロイドの分泌パターン2022

    • Author(s)
      花井匠磨・Tapas Chakraborty・松山倫也・長濱嘉孝・ 太田耕平
    • Organizer
      令和3年度日本水産学会九州支部大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] マサバ Scomber japonicus の体側筋内に移植した生殖腺組織片における配偶子形成2021

    • Author(s)
      八尋逸清・Tapas Chakraborty・太田耕平・松山倫也
    • Organizer
      日本動物学会・九州沖縄植物学会・日本生態学会 合同福岡例会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 新奇海産モデル魚カタクチイワシを利用した不妊化-妊性回復技術の開発2019

    • Author(s)
      甲斐公士.坂口圭史.太田耕平.松山倫也.
    • Organizer
      日本水産増殖学会第 18 回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Luteinizing hormone β-subunit gene knockout in Japanese anchovy ( Engraulis japonicus ) by TALEN-based genome editing2019

    • Author(s)
      Satoshi Kai, Keishi Sakaguchi, Kohei Ohta, Michiya Matsuyama.
    • Organizer
      Marine Biotechnology Conference 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comparative transcriptomics and Edwardsiellosis detection in fish: a study using medaka and Red Sea bream2019

    • Author(s)
      Tapas Chakraborty, Sipra Mohapatra, Sonoko Shimizu, Takahiro Matsubara, Kiyoshi Naruse, Kohei Ohta.
    • Organizer
      10th International conference on Fisheries & Aquaculture, Toronto, 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sex and sex steroids are eminent regulators of fish autophagy.2019

    • Author(s)
      Sipra Mohapatra, Tapas Chakraborty, Kohei Ohta.
    • Organizer
      10th International conference on Fisheries & Aquaculture, Toronto, 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Book] Genome Editing in Fish Reproduction. In: Pandey, P.K., Parhi, J. (eds) Advances in Fisheries Biotechnology.2022

    • Author(s)
      Sipra Mohapatra, Tapas Chakraborty
    • Total Pages
      20
    • Publisher
      Springer Nature Singapore Pte Ltd.
    • ISBN
      9789811632150
    • Related Report
      2020 Annual Research Report
  • [Remarks] 九州大学農学研究院海洋生物学分野HP

    • URL

      https://www.agr.kyushu-u.ac.jp/lab/marinebiology/

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report 2019 Annual Research Report

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Published: 2019-04-18   Modified: 2024-01-30  

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