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Regulation of T-cell activation and cellular immune response by intracellular activation of complement C3 in fish

Research Project

Project/Area Number 19H03050
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionKyushu University

Principal Investigator

NAKAO Miki  九州大学, 農学研究院, 教授 (50212080)

Co-Investigator(Kenkyū-buntansha) 杣本 智軌  九州大学, 農学研究院, 准教授 (40403993)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2021: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2020: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords補体 / C3 / 細胞内活性化 / コイ / 白血球 / 魚類 / カテプシンL / エンドサイトーシス / プロテアーゼ / 遺伝子ノックダウン / 上皮細胞 / 断片化 / アイソタイプ / T細胞
Outline of Research at the Start

本研究は、コイおよびギンブナをモデルとして用い、魚類血球の細胞内におけるC3の活性化機構と、この活性化による免疫応答の制御機構を解明し、補体を介した細胞性免疫応答の強化と制御法を見出すことを目的とする。具体的には、細胞内C3活性化が起こる細胞種の特定および各細胞種においてC3の活性化を担うプロテアーゼの同定を進める。さらに細胞内C3活性化が各細胞種によるサイトカイン産生プロファイルに及ぼす影響を精査する。

Outline of Final Research Achievements

Recently, physiological effects of intracellular complement activation in mammals have attracted much attention, but its phylogenetic development has not been investigated at all. To elucidate the phylogeny and physiological function of intracellular complement activation, this study aimed to detect activated fragments of the complement component C3 in carp leukocyte cells and to identify proteases involved in intracellular C3 fragmentation. The results suggested that C3 is present in carp leukocytes and undergoes limited hydrolysis, which is different from complement activation, on a permanent basis, and that little remains in the unactivated form; that this limited hydrolysis is caused by proteases other than cathepsin L, unlike that reported for human cells; and that C3 may be taken up and fragmented from the extracellular C3 from outside the cell and fragmentation.

Academic Significance and Societal Importance of the Research Achievements

本研究では、プロテアーゼの特定には至らなかったものの、コイPBL内C3の存在と断片化が示された。本研究成果は、学術的には細胞内補体系の系統発生と生理機能の理解を深める重要な知見であるだけでなく、細胞内補体活性化を指標とした、魚類の生体防御能レベルのモニタリング法の開発などの応用につながると期待される。

Report

(4 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (21 results)

All 2022 2021 2020 2019

All Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Open Access: 3 results) Presentation (12 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Journal Article] Development of a filter device for the prevention of aquatic bacterial disease using a single- chain variable fragment (scFv)-conjugated affinity silk2022

    • Author(s)
      Prakash H, Sato M, Kojima K, Sato A, Maruyama S, Nagasawa T, Nakao M, Somamoto T
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 4 Pages: 9475-9475

    • DOI

      10.3390/jmse11040707

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 補体の多面性:生物の進化から探る2022

    • Author(s)
      中尾実樹
    • Journal Title

      日本臨牀

      Volume: 80 Pages: 1723-1727

    • Related Report
      2021 Annual Research Report
  • [Journal Article] 魚類の粘膜免疫と補体2022

    • Author(s)
      中尾実樹
    • Journal Title

      補体

      Volume: 59 Pages: 108-113

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Homeostatic functions of Tecrem, a CD46-like regulatory protein of complement activation, on epithelial cells in carp fish2021

    • Author(s)
      Prakash H, Motobe S, Nagasawa T, Somamoto T, Nakao M
    • Journal Title

      Journal of Marine Science and Engineering

      Volume: 9 Issue: 7 Pages: 687-687

    • DOI

      10.3390/jmse9070687

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Local immune responses to two stages of Ichthiophthirius multifiliis in ginbuna crucian carp2021

    • Author(s)
      Shiota K, Sukeda M, Prakash H, Kondo M, Nakanishi T, Nagasawa T, Nakao M, Somamoto T
    • Journal Title

      Fish & Shellfish Immunology

      Volume: 118 Pages: 19-24

    • DOI

      10.1016/j.fsi.2021.08.013

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lactobacillus paraplantarum L34b-2 derived from fermented food improves the growth, disease resistance and innate immunity in Pangasium bocourti2021

    • Author(s)
      Meidong R, Nakao M, Sakai K, Tongpim S
    • Journal Title

      Aquaculture

      Volume: 531 Pages: 735878-735878

    • DOI

      10.1016/j.aquaculture.2020.735878

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Mixed culture of Bacillus aerius B81e and Lactiplantibacillus paraplantarum L34b-2 derived from in vivo screening using hybrid catfish exhibits high probiotic effects on Pangasius bocourti2021

    • Author(s)
      Meidong R, Buatong A, Nakao M, Sakai K, Tongpim S
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 132 Issue: 4 Pages: 423-428

    • DOI

      10.1016/j.jbiosc.2021.06.006

    • NAID

      40022717523

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Innate cell-mediated cytotoxicity of CD8+ T cells against the protozoan parasite Ichthyophthirius multifiliis in the ginbuna crucian carp, Carassius auratus langsdorfii.2021

    • Author(s)
      Sukeda M, Shiota K, Kondo M, Nagasawa T, Nakao M, Somamoto T
    • Journal Title

      Developmental and Comparative Immunology

      Volume: 115 Pages: 103886-103886

    • DOI

      10.1016/j.dci.2020.103886

    • NAID

      120007188795

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Generation of virus-specific CD8+ T cells by vaccination with inactivated virus in the intestine of ginbuna crucian carp.2019

    • Author(s)
      Tajimi S, Kondo M, Nakanishi T, Nagasawa T, Nakao M, Somamoto T.
    • Journal Title

      Developmental and Comparative Immunology

      Volume: 93 Pages: 37-44

    • DOI

      10.1016/j.dci.2018.12.009

    • NAID

      120006819494

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Mucosal innate defense in carp fish, involving T cells, complement system, and epithelial cells2022

    • Author(s)
      Nakao M
    • Organizer
      The 2nd Congress of Asian Society of Developmental and Comparative Immunology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 細胞内補体活性化の系統発生学的検討:コイ末梢血白血球における補体C3の断片化2022

    • Author(s)
      又吉百音、長澤貴宏、杣本智軌、中尾実樹
    • Organizer
      日本比較免疫学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ドチザメ血清中の抗菌因子の探索と同定2022

    • Author(s)
      白山智恵、長澤貴宏、中尾実樹
    • Organizer
      日本水産学会春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 魚類におけるC3の細胞内活性化の可能性2022

    • Author(s)
      又吉百音、長澤貴宏、杣本智軌、中尾実樹
    • Organizer
      日本補体学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] コイ体表粘液中の補体成分と補体活性化2021

    • Author(s)
      吉迫郁子、長澤貴宏、杣本智軌、中尾実樹
    • Organizer
      日本補体学会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] コイ血清中におけるC3の二量体形成2021

    • Author(s)
      畠山紗矢香、長澤貴宏、杣本智軌、中尾実樹
    • Organizer
      日本補体学会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ドチザメ血清中のザイモサン結合レクチンの同定と発現解析2021

    • Author(s)
      朱 珈凝、友井千帆里、長澤貴宏、杣本智軌、中尾実樹
    • Organizer
      日本比較免疫学会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] イヌザメ血清レクチンの構造・機能分析2020

    • Author(s)
      鳥居爽花・長澤貴宏・中尾実樹
    • Organizer
      日本水産学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 魚類炎症反応におけるC5aの役割解明のための組換えゼブラフィッシュC5aの作製2019

    • Author(s)
      齋藤武尊・長澤貴宏・杣本智軌・中尾実樹
    • Organizer
      日本水産学会秋季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] アフィニティーシルクを用いた魚病細菌除去フィルターの開発2019

    • Author(s)
      Harsha Prakash、丸山真平、佐藤充、小島桂、佐藤巧視、長澤貴宏、中尾実樹、杣本智軌
    • Organizer
      日本魚病学会秋季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ドチザメ血清中のザイモサン結合タンパク質の同定, 日本比較免疫学会学術集会2019

    • Author(s)
      友井千帆里, 長澤貴宏, 濵 虹花, 杣本智軌, 中尾実樹
    • Organizer
      日本比較免疫学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 魚類補体研究の最近の進展と動向2019

    • Author(s)
      中尾実樹
    • Organizer
      日本補体学会学術集会
    • Related Report
      2019 Annual Research Report
    • Invited

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Published: 2019-04-18   Modified: 2024-01-30  

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