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Studies on molecular mechanisms involved in selective toxin accumulation in pufferfish

Research Project

Project/Area Number 19H03051
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionNagasaki University

Principal Investigator

Arakawa Osamu  長崎大学, 水産・環境科学総合研究科(水産), 教授 (40232037)

Co-Investigator(Kenkyū-buntansha) 山田 明徳  長崎大学, 水産・環境科学総合研究科(水産), 准教授 (40378774)
高谷 智裕  長崎大学, 水産・環境科学総合研究科(水産), 教授 (90304972)
山口 健一  長崎大学, 水産・環境科学総合研究科(水産), 教授 (90363473)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2022: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2021: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywordsフグ / テトロドトキシン / サキシトキシン / フグ毒結合タンパク質(PSTBP) / トリブチルスズ結合タンパク質(TBT-bp2) / キタマクラ属 / Pao属 / プロテオフォーム / 麻痺性貝毒 / キタマクラ / オキナワフグ / トラフグ / フグ毒結合タンパク質 / PSTBP / TBT-bp / トリブチルスズ結合タンパク質 / Canthigaster属 / テトロドトキシン結合タンパク質 / マミズフグ / シマキンチャクフグ / TBT-bp2 / Pao cochinchinensis / Pao abei
Outline of Research at the Start

本研究は、海産フグにおいて毒の取り込み・蓄積に関わる血中輸送担体としての機能が示唆されているテトロドトキシン(TTX)/麻痺性貝毒(PST)結合性タンパク質アイソフォーム(Toxin-binding protein related; Tr)群に着目し、毒吸収/蓄積組織におけるTrの膜受容体・毒分子膜輸送体の存在を想定しつつ、海産/淡水フグの各組織を培養して毒の選択的取り込み・蓄積をex vivoで再現し、そこに関わる分子機構や分子進化、そこでのTrの役割を探索するものである。

Outline of Final Research Achievements

In various pufferfish species, the distribution profiles and selective uptake and accumulation abilities of tetrodotoxin (TTX)/saxitoxins (STXs) were investigated, and the distribution and phylogeny of pufferfish toxin binding protein (PSTBP)/tributyltin-binding protein (TBT-bp2) genes, and PSTBP proteoforms were examined. The results newly revealed toxin distribution/selective accumulation profiles for the genus Canthigaster/Pao and euryhaline pufferfish, suggesting that PSTBP is involved in TTX accumulation in pufferfish and TBT-bp2 in STXs accumulation. Furthermore, we were able to elucidate the basic proteoform of tiger pufferfish plasma PSTBP.

Academic Significance and Societal Importance of the Research Achievements

本研究は、フグが何故、如何にして毒をもつようになったのかという水圏生命科学上の根源的な疑問の解明を目指しており、フグ毒化の分子機構のみならず、海水と淡水で異なる毒を獲得してきたフグの進化の過程にも迫るものである。さらに、食品衛生上世界的な問題となっている魚介類のTTX/STXs汚染リスクを根本的に回避するうえでも、重要な知見を提供するだろう。今回得られた成果は、フグの毒化機構の解明に大きく貢献するものであり、将来的にはゲノム編集技術によるスーパー無毒フグ(遺伝的に毒を蓄積しないフグ)の作出などにも応用可能と考えられる。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (18 results)

All 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (2 results) Journal Article (11 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 8 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (1 results)

  • [Int'l Joint Research] University of Kratie(カンボジア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Gyeongsang National University(韓国)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Tetrodotoxin Profiles in Xanthid Crab Atergatis floridus and Blue-Lined Octopus Hapalochlaena cf. fasciata from the Same Site in Nagasaki, Japan2023

    • Author(s)
      Yuchengmin Zhang、Yuta Yamate、Takeshi Takegaki、Osamu Arakawa、Tomohiro Takatani
    • Journal Title

      Toxins

      Volume: 15 Issue: 3 Pages: 193-193

    • DOI

      10.3390/toxins15030193

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] フグ毒保有魚類の成長や成熟に伴う毒蓄積組織の変動2023

    • Author(s)
      辰野竜平、高谷智裕、荒川 修
    • Journal Title

      月刊海洋

      Volume: 55 Pages: 17-21

    • Related Report
      2022 Annual Research Report
  • [Journal Article] カニの毒 - フグ毒と麻痺性貝毒の蓄積, 分布, 起源2023

    • Author(s)
      高谷智裕、張宇騁旻、筒井英人、荒川 修
    • Journal Title

      月刊海洋

      Volume: 55 Pages: 43-46

    • Related Report
      2022 Annual Research Report
  • [Journal Article] フグにおけるフグ毒と麻痺性貝毒の選択性2023

    • Author(s)
      荒川 修、山田明徳、山口健一、高谷智裕
    • Journal Title

      月刊海洋

      Volume: 55 Pages: 70-75

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Complete mitochondrial genomes of the Southeast Asian freshwater pufferfishes, Pao abei (Roberts, 1998) and Pao suvattii (Sontirat and Soonthornsatit, 1985) (Tetraodontiformes: Tetraodontidae) and an insight into the taxonomic status of Pao species2021

    • Author(s)
      Yamada Akinori、Hamaguchi Ayaka、Sakoda Hikari、Kakamu Motohiro、Doi Hiroyuki、Hasin Sasitorn、Arakawa Osamu
    • Journal Title

      Mitochondrial DNA Part B

      Volume: 6 Issue: 4 Pages: 1448-1450

    • DOI

      10.1080/23802359.2021.1911708

    • NAID

      120007163291

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Tetrodotoxin/Saxitoxins Selectivity of the Euryhaline Freshwater Pufferfish Dichotomyctere fluviatilis2021

    • Author(s)
      Zhu Hongchen、Sakai Towa、Nagashima Yuji、Doi Hiroyuki、Takatani Tomohiro、Arakawa Osamu
    • Journal Title

      Toxins

      Volume: 13 Issue: 10 Pages: 731-731

    • DOI

      10.3390/toxins13100731

    • NAID

      120007180584

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Geographic Variations in the Toxin Profile of the Xanthid Crab Zosimus aeneus in a Single Reef on Ishigaki Island, Okinawa, Japan2021

    • Author(s)
      Zhang Yuchengmin、Tsutsui Hideto、Yamawaki Nobuhiro、Morii Yasuhiro、Nishihara Gregory N.、Itoi Shiro、Arakawa Osamu、Takatani Tomohiro
    • Journal Title

      Marine Drugs

      Volume: 19 Issue: 12 Pages: 670-670

    • DOI

      10.3390/md19120670

    • NAID

      120007186851

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Co-occurrence of tetrodotoxin and saxitoxins and their intra-body distribution in the pufferfish Canthigaster valentini2020

    • Author(s)
      Zhu Hongchen, Sonoyama Takayuki、Yamada Misako、Gao Wei、Tatsuno Ryohei、Takatani Tomohiro、Arakawa Osamu
    • Journal Title

      Toxins

      Volume: 12 Issue: 7 Pages: 436-436

    • DOI

      10.3390/toxins12070436

    • NAID

      120006988125

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phylogeny and Toxin Profile of Freshwater Pufferfish (Genus Pao) Collected from 2 Different Regions in Cambodia.2020

    • Author(s)
      Zhu, H., Yamada, A., Goto, Y., Horn, L., Ngy, L., Wada, M., Doi, H., Lee, JS., Takatani, T., Arakawa, O
    • Journal Title

      Toxins

      Volume: 12 Issue: 11 Pages: 689-703

    • DOI

      10.3390/toxins12110689

    • NAID

      120006988469

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evaluation of the tetrodotoxin uptake ability of pufferfish Takifugu rubripes according to age using an in vitro tissue slice incubation method.2020

    • Author(s)
      W. Gao, M. Yamada, R. Ohki, Y. Nagashima, R. Tatsuno, K. Ikeda, K. Kawatsu, T. Takatani, O. Arakawa
    • Journal Title

      Toxicon

      Volume: 174 Pages: 8-12

    • DOI

      10.1016/j.toxicon.2019.11.014

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Contrasting Toxin Selectivity between the Marine Pufferfish Takifugu pardalis and the Freshwater Pufferfish Pao suvattii2019

    • Author(s)
      Gao Wei、Kanahara Yoko、Yamada Misako、Tatsuno Ryohei、Yoshikawa Hiroyuki、Doi Hiroyuki、Takatani Tomohiro、Arakawa Osamu
    • Journal Title

      Toxins

      Volume: 11 Issue: 8 Pages: 470-470

    • DOI

      10.3390/toxins11080470

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Phylogeny and toxin profile of two Cambodian freshwater pufferfish species of the genus Pao2022

    • Author(s)
      H. Zhu、A. Yamada、Y. Goto、J. Takeoka、L. Horn、L. Ngy、M. Wada、H. Doi、J. S. Lee、T. Takatani、O. Arakawa
    • Organizer
      The XVIth International Congress of Toxicology
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] フグ毒結合タンパク質アイソフォームのプロテオーム解析2022

    • Author(s)
      南 良直, Zhang Yafei, 山口健一, 大嶋雄治, 山田明徳, 高谷智裕, 荒川 修
    • Organizer
      令和4年度日本水産学会春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Tetrodotoxin/saxitoxin selectivity in pufferfish2021

    • Author(s)
      O. Arakawa, A. Yamada, K. Yamaguchi, T. Takatani
    • Organizer
      6th Asia-Pacific Symposium on Food Safety 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 人工種苗トラフグの孵化後初期におけるTTX量の変化2019

    • Author(s)
      五十川純太、鈴木重則、荒川 修、高谷智裕
    • Organizer
      令和元年度日本水産学秋季大会
    • Related Report
      2019 Annual Research Report
  • [Book] 無毒フグ肝を食用化する2022

    • Author(s)
      野口玉雄、濱田英嗣、荒川 修
    • Total Pages
      143
    • Publisher
      筑波書房
    • ISBN
      9784811906188
    • Related Report
      2021 Annual Research Report

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Published: 2019-04-18   Modified: 2024-01-30  

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