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New strategies for controlling silage microbiota using integrated analysis of bacteriome and mycobiome

Research Project

Project/Area Number 19H03106
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 42010:Animal production science-related
Research InstitutionOkayama University

Principal Investigator

Nishino Naoki  岡山大学, 環境生命科学学域, 教授 (50237715)

Co-Investigator(Kenkyū-buntansha) 鶴田 剛司  岡山大学, 環境生命科学学域, 准教授 (90728411)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2022: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2021: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Keywords飼料 / サイレージ / 微生物 / 微生物叢 / アンプリコン解析 / 発酵 / 細菌叢 / 真菌叢
Outline of Research at the Start

アンプリコン解析、予測メタゲノム、ネットワーク解析という新しいアプローチで、サイレージの微生物制御に革新的な知見を提供する。実態調査を基本に研究を進め、細菌と真菌を組合わせた混合微生物製剤のパイロット試験まで実施する。微生物叢の統合的理解およびそれを基にしたサイレージ調製の高度化手法を提示することが到達目標である。

Outline of Final Research Achievements

The objectives of this study were 1) to determine whether understanding the metabolic functions that fluctuate with the bacterial and fungal microbiota can provide valuable breakthroughs to unresolved problems in silage production and utilization, 2) to determine whether the interactions between the bacterial and fungal microbiota can provide innovative insights into the prevention of aerobic deterioration, and 3) to develop a mixed microbial inoculant based on the integrated analyses of bacterial and fungal microbiota. We obtained novel and valuable results on the research for 1) and 2), but the results for 3) still needed to be improved in demonstrating the usefulness of integrated analyses. It was also reaffirmed that understanding practical-scale silos is more complicated than in laboratory silos, and we would like to continue this research to further improve the value of our research.

Academic Significance and Societal Importance of the Research Achievements

DNA情報を利用した微生物叢の解析技術は、過去20年ほどの間に著しく高度化ならびに汎用化した。収穫・梱包機械の改良も続けられており、総体的な品質管理技術は着実に向上していると言ってよい。一方、暖地型草種の酢酸発酵、好気的変敗の防止のように、未だに解決されていない問題もある。これらの技術的頭打ちに対し、本研究では細菌叢と真菌叢の統合的解析というアプローチでブレークスルーを試みた。暖地型牧草サイレージ、発酵TMR、新規乳酸菌製剤の評価には有効であったが、実規模のトウモロコシホールクロップサイレージの理解には不十分であった。さらなる改良、改善を加えて研究を継続したい。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (18 results)

All 2023 2022 2021 2020 2019

All Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 5 results) Presentation (10 results) (of which Int'l Joint Research: 7 results)

  • [Journal Article] Monitoring the milk composition, milk microbiota, and blood metabolites of Jersey cows throughout a lactation period.2023

    • Author(s)
      Gathinji, P.K., Yousofi, Z., Akada, K., Wali, A. & Nishino, N.
    • Journal Title

      Veterinary Sciences

      Volume: 10 Issue: 3 Pages: 226-226

    • DOI

      10.3390/vetsci10030226

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fecal, milk, uterine, airborne dust, and water microbiota in dairy farms in southern Vietnam: A pilot study.2022

    • Author(s)
      Tran, T.M.T., Tran, H.D, Nguyen, T.T., Acosta, T.J., Tsuruta, T., Nishino, N. & Duong, T.H.
    • Journal Title

      Advances of Animal and Veterinary Sciences

      Volume: 10 Issue: 7 Pages: 1525-1525

    • DOI

      10.17582/journal.aavs/2022/10.7.1525.1531

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Frequency of β-casein gene polymorphisms in Jersey cows in western Japan.2022

    • Author(s)
      Nuomin, Nguyen, Q.D., Aodaohu & Nishino, N.
    • Journal Title

      Animals

      Volume: 12 Issue: 16 Pages: 2076-2076

    • DOI

      10.3390/ani12162076

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bacterial and fungal microbiota of total mixed ration silage stored at various temperatures2022

    • Author(s)
      Wali, A., Hou, J., Tsuruta, T., and Nishino, N.
    • Journal Title

      Journal of Applied Microbiology

      Volume: - Issue: 2 Pages: 1-12

    • DOI

      10.1111/jam.15582

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bacterial and fungal microbiota of guinea grass silage shows various levels of acetic acid fermentation2022

    • Author(s)
      Hou, J. and Nishino, N.
    • Journal Title

      Fermentation

      Volume: 8 Issue: 1 Pages: 10-10

    • DOI

      10.3390/fermentation8010010

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bacterial and fungal microbiota associated with the ensiling of wet soybean curd residue under prompt and delayed sealing conditions2020

    • Author(s)
      Wali Ajmal, Nishino Naoki
    • Journal Title

      Microorganisms

      Volume: 8 Issue: 9 Pages: 1334-1334

    • DOI

      10.3390/microorganisms8091334

    • NAID

      120006900790

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Examination of milk microbiota, fecal microbiota, and blood metabolites of Jersey cows in cool and hot seasons2020

    • Author(s)
      Nguyen Dang Qui, Tsuruta Takeshi, Nishino Naoki, Q.D., Tsuruta, T., Nishino, N.
    • Journal Title

      Animal Science Journal

      Volume: 1 Issue: 1

    • DOI

      10.1111/asj.13441

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The relationship between uterine, fecal, bedding, and airborne dust microbiota from dairy cows and their environment: A pilot study2019

    • Author(s)
      Thuong T. Nguyen, Ayumi Miyake, Tu T.M. Tran, Takeshi Tsuruta, and Naoki Nishino
    • Journal Title

      Animals

      Volume: 9 Issue: 12 Pages: 1007-1007

    • DOI

      10.3390/ani9121007

    • NAID

      120006816635

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Inhibited aerobic spoilage by multiple lactic acid bacteria inhabiting total mixed ration silage.2022

    • Author(s)
      Wali, A., Hou, J., Tran, D.P., Ton, N.M.T. & Nishino, N.
    • Organizer
      Fourth International Conference on Sustainable Agriculture and Environment
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Milk microbiota, milk composition, and blood metabolites of Jersey cows throughout a lactation period.2022

    • Author(s)
      Gathinji, P.K., Zabialla, Y., Nguyen D.Q., Aodaofu & Nishino, N.
    • Organizer
      Fourth International Conference on Sustainable Agriculture and Environment
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The effect of protein sources on gut microbiota in mice with and without osmotic diarrhea.2022

    • Author(s)
      Nuomin, Baek, R., Tran, D.P., Ton, N.M.T., Tsuruta, T. & Nishino, N.
    • Organizer
      Fourth International Conference on Sustainable Agriculture and Environment
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Production of β-casomorphin from A1 milk, A2 milk, A1 yogurt and A2 yogurt2022

    • Author(s)
      Baek, R., Nuomin, Ton, N.M.T., Kiyochika, T., Tsuruta, T. & Nishino, N.
    • Organizer
      Fourth International Conference on Sustainable Agriculture and Environment
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 牧草類の嫌気的貯蔵に関わる細菌叢と真菌叢2022

    • Author(s)
      侯建建・Wali Ajmal・西野直樹
    • Organizer
      日本草地学会2022年度大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Bacterial and fungal microbiota of guinea grass silage stored at moderate and high ambient temperatures with and without wilting2021

    • Author(s)
      Hou, J., Nishino, N., and Tsuruta, T.
    • Organizer
      International Symposium on Animal Bioscience
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 酢酸型発酵を示したギニアグラスサイレージの細菌叢と真菌叢2021

    • Author(s)
      Hou JianJian・西野直樹
    • Organizer
      2021年度日本草地学会大会(第77回発表会)
    • Related Report
      2020 Annual Research Report
  • [Presentation] 貯蔵温度による発酵TMRの細菌叢および真菌叢の変動2021

    • Author(s)
      Wali Ajmal・西野直樹
    • Organizer
      2021年度日本草地学会大会(第77回発表会)
    • Related Report
      2020 Annual Research Report
  • [Presentation] Fermentation and microbiota of guinea grass silage stored at two different temperatures2020

    • Author(s)
      Hou JianJian, Wali Ajmal, Tsuruta Takeshi, Nishino Naoki
    • Organizer
      International Symposium on Symposium on Application of Advanced Technologies In Agriculture
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Acceptable storability and aerobic stability of by-products ensiled as a total mixed ration2020

    • Author(s)
      Wali Ajmal, Hou JianJian, Tsuruta Takeshi, Nishino Naoki
    • Organizer
      International Symposium on Symposium on Application of Advanced Technologies In Agriculture
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research

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Published: 2019-04-18   Modified: 2024-01-30  

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