• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Versatile molecular functions of the ribosomal stalk protein in translation cycle

Research Project

Project/Area Number 19H03155
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 43010:Molecular biology-related
Research InstitutionNiigata University

Principal Investigator

Uchiumi Toshio  新潟大学, 自然科学系, フェロー (50143764)

Co-Investigator(Kenkyū-buntansha) 伊東 孝祐  新潟大学, 自然科学系, 准教授 (20502397)
西川 周一  新潟大学, 自然科学系, 教授 (10252222)
石野 園子  九州大学, 農学研究院, 准教授 (80399740)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2021: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2020: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywordsリボソーム / リボソーム蛋白質 / リボソームストーク / 翻訳因子 / 蛋白質合成 / EF1A / EF1B / YchF / リボソームストーク蛋白質 / 翻訳 / タンパク質合成 / リボソームタンパク質
Outline of Research at the Start

遺伝情報の翻訳反応(蛋白質合成)は、リボソームと様々な翻訳因子の相互作用を介して正確に進行する。リボソームを構成する蛋白質の一部である“ストーク蛋白質”は、この相互作用の効率と調節に関わることが知られているが、その詳細な分子機構は明確にされていない。本研究では、翻訳反応の材料となるアミノ酸をtRNAを介してリボソームにもたらす因子であるaEF1Aとストーク蛋白質間相互作用とその調節機構、さらに新規に見出された機能未知の因子aYchFとストーク蛋白質間の相互作用とその役割について解明し、これまで得られている知見と併せて、ストーク蛋白質の動的で多彩なはたらきの全貌を分子レベルで解明する。

Outline of Final Research Achievements

Translation of genetic information proceeds on the ribosome with amino acids being brought efficiently to the ribosome by the translation factor EF1A one after another. There are many unclear points about the mechanism for achieving the high efficiency. In this project, we have demonstrated that the ribosomal stalk protein contributes to translation efficiency by binding, via its C-terminal region, to two different conformations of EF1A, that is, the active EF1A-GTP form that carries the aminoacyl-tRNA to the ribosome and the inactive EF1A-GDP form that is dissociated from the ribosome, and that the interaction between the stalk and EF1A is disrupted by anothor factor EF1B which promote nucleotide exchange from EF1A-GDP to EF1A-GTP. Furthermore, we also detected ability of the stalk to bind to a stress-response factor, YchF. These results represent the versatile functions of the ribosomal stalk in translation efficiency and control.

Academic Significance and Societal Importance of the Research Achievements

本研究では一部のリボソーム蛋白質(ストーク)が翻訳因子EF1Aと直接相互作用し、リボソームに効率よくアミノ酸・tRNAを次々に運搬するはたらきを担うこと、また他の因子EF1BがストークとEF1A間相互作用を調節し、不活性型EF1A・GDPから活性型EF1A・GTPに変換することを示し、ストーク/EF1A/EF1Bの連携作用が蛋白質合成の効率化をもたらすことを証明した。また、ストークがストレス応答因子YchFのリボソーム結合にも関与することを立証し、蛋白質合成機構へのストークの多彩なはたらきが示された。蛋白質合成制御の研究ばかりでなく基礎医学や応用生命科学の分野にも広く影響を与える内容である。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (23 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (2 results) Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 5 results) Presentation (11 results) (of which Invited: 3 results) Remarks (2 results)

  • [Int'l Joint Research] The Chinese University of Hong Kong(中国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] The chinese University of Hong Kong(中国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] The flexible N-terminal motif of uL11 unique to eukaryotic ribosomes interacts with P-complex and facilitates protein translation2022

    • Author(s)
      L. Yang, K.-M. Lee, C. W.-H. Yu, H. Imai, K. Ito, T. Uchiumi, K.-B. Wong et al.
    • Journal Title

      Nucleic Acids Res.

      Volume: -

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A novel ribosome-dimerization protein found in the hyperthermophilic archaeon Pyrococcus furiosus using ribosome-associated proteomics2022

    • Author(s)
      C. Yaeshima, N. Murata, S. Ishino, I. Sagawa, K. Ito, T. Uchiumi
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 593 Pages: 116-121

    • DOI

      10.1016/j.bbrc.2022.01.043

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural insights into the switching off of the interaction between the archaeal ribosomal stalk and aEF1A by nucleotide exchange factor aEF1B.2021

    • Author(s)
      Suzuki,T., Ito, K., Miyoshi, T., Murakami, R., Uchiumi, T.
    • Journal Title

      J. Mol. Biol.

      Volume: - Issue: 15 Pages: 167046-167046

    • DOI

      10.1016/j.jmb.2021.167046

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mood Disorder in Systemic Lupus Erythematosus Induced by Antiribosomal P Protein Antibodies Associated with Decreased Serum and Brain Tryptophan.2021

    • Author(s)
      Cho, T., Sato, H., Wakamatsu, A., Ohashi, R., Ajioka, Y., Uchiumi, T., Goto, S., Narita, I., Kaneko, Y.
    • Journal Title

      J. Immunol.

      Volume: 206 Issue: 8 Pages: 1729-1739

    • DOI

      10.4049/jimmunol.2000260

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Direct visualization of translational GTPase factor pool formed around the archaeal ribosomal P-stalk by high-speed AFM2020

    • Author(s)
      Imai Hirotatsu、Uchiumi Toshio、Kodera Noriyuki
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 117 Issue: 51 Pages: 32386-32394

    • DOI

      10.1073/pnas.2018975117

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Switch of the interactions between the ribosomal stalk and EF1A in the GTP- and GDP-bound conformations2019

    • Author(s)
      Maruyama K., Imai H., Kawamura M., Ishino S., Ishino Y., Ito K., Uchiumi T.
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 14761-14761

    • DOI

      10.1038/s41598-019-51266-x

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural and mutagenesis studies evince the role of the extended protuberant domain of ribosomal protein uL10 in protein translation2019

    • Author(s)
      Choi K.A., Yang L., Lee K.M., Yu C.W., Banfield D.K., Ito K., Uchiumi T., Wong K.B.
    • Journal Title

      Biochemistry

      Volume: 58 Issue: 36 Pages: 3744-3754

    • DOI

      10.1021/acs.biochem.9b00528

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] High-resolution crystal structure of peptidyl-tRNA hydrolase from Thermus thermophiles2019

    • Author(s)
      Matsumoto A., Uehara Y., Shimizu Y., Ueda T., Uchiumi T., Ito K.
    • Journal Title

      Proteins

      Volume: 87 Issue: 3 Pages: 226-235

    • DOI

      10.1002/prot.25643

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] リボソームストークタンパク質とEF1Aの相互作用はEF1Bにより解消される2022

    • Author(s)
      鈴木隆寛、伊東孝祐、三好智博、村上僚、内海利男
    • Organizer
      第16回無細胞生命科学研究会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Direct visualization of translational GTPase factor pool formed around the archaeal ribosomal P-stalk by high-speed AFM2021

    • Author(s)
      今井 大達, 内海 利男, 古寺 哲幸
    • Organizer
      第22回日本RNA学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Structural and functional analysis of the interaction between family D-DNA polymerase and CMG-like helicase in the replisome of Thermococcus kodakarensis2021

    • Author(s)
      沖 啓輔、永田麻梨子、山上 健、沼田倫征、石野園子、大山拓 次、石野良純
    • Organizer
      第44回日本分子生物学会年会 Workshop “What is life in a microbe?”
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 有性生殖過程の核融合を阻害する化合物の探索と解析2021

    • Author(s)
      小林那奈美、斎藤圭吾、佐藤綾人、伊丹健一郎、西川周一
    • Organizer
      新潟生化学懇話会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ペプチジルtRNA加水分解酵素Pth2・tRNA複合体の構造解析2021

    • Author(s)
      茅原真晃、今井大達、内海 利男、伊東 孝祐
    • Organizer
      第15回無細胞生命科学研究会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 高速原子間力顕微鏡による古細菌リボソームストーク複合体上に形成されたGTPase翻訳因子プールの可視化2020

    • Author(s)
      今井 大達、内海 利男、古寺 哲幸
    • Organizer
      第43回日本分子生物学学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Visualization of translational GTPase factor-pool formed around the archaeal ribosomal P-stalk by HS-AFM2020

    • Author(s)
      今井 大達、内海 利男、古寺 哲幸
    • Organizer
      日本生物物理学会第58回年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 有性生殖過程の核融合を阻害する化合物の探索2020

    • Author(s)
      小林那奈美、斉藤圭吾、佐藤綾人、伊丹健一郎、西川周一
    • Organizer
      植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] リボソームストークタンパク質の構造・機能研究2020

    • Author(s)
      伊東孝祐、丸山圭、今井大達、内海利男
    • Organizer
      第93回日本細菌学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] リボソーム・翻訳因子間の結合と解離に寄与するストーク蛋白質の構造可変性2019

    • Author(s)
      伊東孝祐、丸山圭、今井大達、内海利男
    • Organizer
      第42回日本分子生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 超好熱アーキアThermococcus kodakarensisのoriCおよびorc1/cdc6欠失変異体の単離とその性質2019

    • Author(s)
      石野園子、松見理恵、John N. Reeve、石野良純
    • Organizer
      第42回日本分子生物学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Remarks] Uchiumi-Ito Lab

    • URL

      https://www.sc.niigata-u.ac.jp/biologyindex/uchiumi-ito/

    • Related Report
      2021 Annual Research Report
  • [Remarks] Uchiumi-Ito Lab.

    • URL

      https://www.sc.niigata-u.ac.jp/biologyindex/uchiumi-ito/

    • Related Report
      2019 Annual Research Report

URL: 

Published: 2019-04-18   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi