Mechanism of regulation of gene expression levels enabled by diversity in histone composition.
| Project/Area Number |
19H03158
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 43010:Molecular biology-related
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2021: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2020: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2019: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
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| Keywords | ヒストンバリアント / クロマチン / ホッジ分解 / エネルギー地形 |
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| Outline of Research at the Start |
本研究では、ホッジ分解と呼ばれる数理的手法を応用した一細胞データに内在する「多層的な時間構造」を抽出する技術を開発する。これを独自に開発した少数細胞エピゲノムプロファイル法により取得した骨格筋の分化・再生過程の一細胞データに時間構造を与える。そして、データ駆動的にクロマチン構造変化のダイナミクスをエネルギー地形として再構築する。これらのアプローチにより、細胞分化におけるクロマチン構造変化を時空間的に理解することで、ヒストン組成の多様性が可能とする遺伝子発現量制御機構の解明を目指す。
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| Outline of Final Research Achievements |
We established a genome-wide chromatin profiling technique for small tissue sections by optimizing ChIL technology to analyze the relationship between histone composition and differentiation process in muscle tissue in detail. We also developed a data analysis method to reconstruct energy landscapes from single cell data. We further developed the ChIL method and extended it to a method using two antibodies simultaneously. We are attempting to apply this method to the extraction of chromatin dynamics during differentiation by looking at the two different molecules in the same cell. The analyses of novel histone variants were published mainly through collaborative research.
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| Academic Significance and Societal Importance of the Research Achievements |
細胞分化におけるクロマチン構造変化を時空間的に解析する技術を確立することで、ヒストン組成の多様性が可能とする遺伝子発現量制御機構の解明が期待できる。データ駆動的なダイナミクス抽出は、他分野のデータ解析に応用可能である。また、単一細胞遺伝子発現データを対象とする新規細胞プロファイリング法は、従来の個別かつ大量の遺伝子の統計的検定では見えなかった複数遺伝子間の依存関係を含む新しい遺伝子機能の発見が期待できる。
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Relayed signaling between mesenchymal progenitors and muscle stem cells ensures adaptive stem cell response to increased mechanical load.2021
Author(s)
Kaneshige A, Kaji T, Zhang L, Saito H, Nakamura A, Kurosawa T, Ikemoto-Uezumi M, Tsujikawa K, Seno S, Hori M, Saito Y, Matozaki T, Maehara K, Ohkawa Y, Potente M, Watanabe S, Braun T, Uezumi A, Fukada SI.
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Journal Title
Cell Stem Cell.
Volume: 29
Issue: 2
Pages: 265-280
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Modeling population size independent tissue epigenomes by ChIL-seq with single thin sections.2021
Author(s)
Maehara K, Tomimatsu K, Harada A, Tanaka K, Sato S, Fukuoka M, Okada S, Handa T, Kurumizaka H, Saitoh N, Kimura H, Ohkawa Y.
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Journal Title
Mol Syst Biol.
Volume: 17
Related Report
Peer Reviewed / Open Access
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[Journal Article] Sustained expression of HeyL is critical for the proliferation of muscle stem cells in overloaded muscle.2019
Author(s)
Fukuda S, Kaneshige A, Kaji T, Noguchi YT, Takemoto Y, Zhang L, Tsujikawa K, Kokubo H, Uezumi A, Maehara K, Harada A, Ohkawa Y, Fukada SI.
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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