| Budget Amount *help |
¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2022: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2021: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2020: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Outline of Final Research Achievements |
This study aimed to explain the correlation between genome reduction and growth rate in Escherichia coli by the chromosomal periodicity of gene expression. We comprehensively measured E. coli growth, experimental evolution, genome mutation, and gene expression analysis. As a result, we found that the chromosomal periods of gene expression maintained conserved; however, the periodic amplitude and phase differed from that of the full-length genome (wild type). Such divergence led to the decreased growth rates of reduced genomes. Experimental evolution restored the growth rates of genome-reduced strains, correlated to the recovery of the chromosomal periodicity of gene expression. In summary, bacterial growth was globally determined by the periodic order of the transcriptome.
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