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Studies on plant signaling pathways involved in negative regulation of root nodule symbiosis

Research Project

Project/Area Number 19H03239
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionUniversity of Tsukuba

Principal Investigator

Suzaki Takuya  筑波大学, 生命環境系, 准教授 (40575825)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2021: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2020: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords根粒共生 / 植物微生物相互作用 / 転写因子 / ミヤコグサ / シグナル伝達 / マメ科植物 / 窒素 / 硝酸 / 根粒形成 / トランスポーター / 窒素応答 / 窒素栄養 / 長距離シグナル伝達 / 硝酸応答
Outline of Research at the Start

マメ科植物は、窒素栄養が十分量存在する時は根粒共生を抑制することで共生にともなう炭素源の不要な損失を防いでいる。本現象は、栄養環境に応答した植物の器官形成を端的に示す事例の1つと言えるが、その分子機構は不明な点が多い。本研究課題では、マメ科のモデル植物ミヤコグサを用いて本現象に関わる長距離移動性のペプチド性シグナル分子の特定および機能解明と、2つの転写因子を介した遺伝子発現制御機構の解明に関する研究を推進する。得られた知見から、窒素栄養に応答した根粒共生抑制機構を統合的に理解することを目指す。

Outline of Final Research Achievements

It is known that legumes prevent unnecessary loss of carbon source due to symbiosis by suppressing root nodule symbiosis (RNS) in the presence of sufficient nitrogen nutrients. Hoewever, the molecular basis of the mechanisms remains unclear. In this research project, using Lotus japonicus, we found that a signaling pathway mediated by two NLP transcription factors (LjNLP1/4) and the nitrate transporter LjNRT2.1 plays a central role in the negative regulation of RNS in response to nitrate. We also identified novel peptides that function in response to nitrogen and revealed that they play essential roles for the establishment of RNS. These findings have deepened our understanding of the mechanisms of plant adaptation to nitrogen environments.

Academic Significance and Societal Importance of the Research Achievements

持続可能な社会の実現に向けて、肥料に頼らない農業展開が世界的な喫緊の課題である。ダイズやエンドウマメなどのマメ科植物は窒素固定細菌の根粒菌と共生関係を築くことで、空中窒素を肥料として利用することができる。本研究課題は植物と根粒菌の共生の仕組み及び窒素栄養に対する植物の応答の仕組みの一端を明らかにした。得られた成果に基づいて今後研究を深めていくことによって、低施肥農業の実現や貧栄養の土地での農業実現が期待される。

Report

(4 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (43 results)

All 2023 2022 2021 2020 2019

All Journal Article (12 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 8 results) Presentation (31 results) (of which Int'l Joint Research: 6 results,  Invited: 14 results)

  • [Journal Article] Root nodule organogenesis: a unique lateral organogenesis in legumes2023

    • Author(s)
      Takuya Suzaki
    • Journal Title

      Breeding Science

      Volume: 73 Issue: 1 Pages: 70-75

    • DOI

      10.1270/jsbbs.22067

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] <i>Lotus japonicus</i> NLP1 and NLP4 transcription factors have different roles in the regulation of nitrate transporter family gene expression2022

    • Author(s)
      Hanna Nishida、Takuya Suzaki
    • Journal Title

      Genes & Genetic Systems

      Volume: 97 Issue: 5 Pages: 257-260

    • DOI

      10.1266/ggs.22-00104

    • ISSN
      1341-7568, 1880-5779
    • Year and Date
      2022-10-01
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nitrate transport via NRT2.1 mediates NIN-LIKE PROTEIN-dependent suppression of root nodulation in Lotus japonicus2022

    • Author(s)
      Misawa Fumika、Ito Momoyo、Nosaki Shohei、Nishida Hanna、Watanabe Masahiro、Suzuki Takamasa、Miura Kenji、Kawaguchi Masayoshi、Suzaki Takuya
    • Journal Title

      The Plant Cell

      Volume: 34 Issue: 5 Pages: 1844-1862

    • DOI

      10.1093/plcell/koac046

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Different DNA-binding specificities of NLP and NIN transcription factors underlie nitrate-induced control of root nodulation2021

    • Author(s)
      Nishida Hanna、Nosaki Shohei、Suzuki Takamasa、Ito Momoyo、Miyakawa Takuya、Nomoto Mika、Tada Yasuomi、Miura Kenji、Tanokura Masaru、Kawaguchi Masayoshi、Suzaki Takuya
    • Journal Title

      The Plant Cell

      Volume: online published Issue: 7 Pages: 2340-2359

    • DOI

      10.1093/plcell/koab103

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Editorial: Nutrient Dependent Signaling Pathways Controlling the Symbiotic Nitrogen Fixation Process2021

    • Author(s)
      Suzaki Takuya、Valkov Vladimir Totev、Chiurazzi Maurizio
    • Journal Title

      Frontiers in Plant Science

      Volume: 12 Pages: 744450-744450

    • DOI

      10.3389/fpls.2021.744450

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] MIR2111-5 locus and shoot-accumulated mature miR2111 systemically enhance nodulation depending on HAR1 in Lotus japonicus.2020

    • Author(s)
      Okuma N, Soyano T, Suzaki T, Kawaguchi, M.
    • Journal Title

      Nat Commun.

      Volume: 11 Issue: 1 Pages: 5192-5192

    • DOI

      10.1038/s41467-020-19037-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 微生物・植物相互作用2020

    • Author(s)
      別役重之,寿崎拓哉
    • Journal Title

      生物工学

      Volume: 98 Pages: 309-313

    • Related Report
      2020 Annual Research Report
  • [Journal Article] CLE-HAR1 systemic signaling and NIN-mediated local signaling suppress the increased rhizobial infection in the daphne mutant of Lotus japonicus2020

    • Author(s)
      Yoro E., Suzaki T., and Kawaguchi M.
    • Journal Title

      Molecular Plant-Microbe Interaction

      Volume: 33 Issue: 2 Pages: 320-327

    • DOI

      10.1094/mpmi-08-19-0223-r

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Autoregulation of nodulation pathway is dispensable for nitrate-induced control of rhizobial infection2020

    • Author(s)
      Nishida, H., Ito, M., Miura K., Kawaguchi, M. and Suzaki, T.
    • Journal Title

      Plant Signaling & Behavior

      Volume: 15 Issue: 3 Pages: 1733814-1733814

    • DOI

      10.1080/15592324.2020.1733814

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Agroinfiltration-based efficient transient protein expression in leguminous plants2019

    • Author(s)
      Suzaki T., Tsuda M., Ezura H., Day B., and Miura K.
    • Journal Title

      Plant Biotechnology

      Volume: 36 Issue: 2 Pages: 119-123

    • DOI

      10.5511/plantbiotechnology.19.0220b

    • NAID

      130007672672

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2019-06-25
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Autoregulation of legume nodulation by sophisticated transcription regulatory networks2019

    • Author(s)
      Suzaki T., and Nishida H.
    • Journal Title

      Molecular Plant

      Volume: 12 Issue: 9 Pages: 1179-1181

    • DOI

      10.1016/j.molp.2019.07.008

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 硝酸に応答して根粒共生を制御する植物の転写因子2019

    • Author(s)
      西田帆那、寿崎拓哉
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 77 Pages: 216-220

    • Related Report
      2019 Annual Research Report
  • [Presentation] Adaptive mechanisms of leguminous plants to nitrogen environment2023

    • Author(s)
      Suzaki Takuya
    • Organizer
      38th IPSR International Symposium
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 根粒形成の鍵転写因子NINの機能未知領域に関する研究2023

    • Author(s)
      野田 桃菜; 西田 帆那; 伊藤 百代; 壽崎 拓哉
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 根粒形成を促進する鍵転写因子のDNA結合特性に関する研究2023

    • Author(s)
      野崎 翔平; 野田 桃菜; 三浦 謙治; 壽崎 拓哉
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 硝酸態窒素による根粒形成制御に関わる自然変異の同定2023

    • Author(s)
      伊藤 百代; 番場 大; 陳 漢謀; 野崎 翔平; 田島 由理; 三浦 謙治; 佐藤 修正; 壽崎 拓哉
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 窒素栄養に応じた全身的・根局所的な根粒形成制御2022

    • Author(s)
      壽崎 拓哉
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 硝酸態窒素に応じた根粒共生制御の分子機構2022

    • Author(s)
      壽崎 拓哉
    • Organizer
      植物の栄養研究会第7回研究交流会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 根粒形成開始マスターレギュレーターNIN 転写因子の根粒成熟過程における機能2022

    • Author(s)
      野田 桃菜; 西田 帆那; 伊藤 百代; 壽崎 拓哉
    • Organizer
      植物の栄養研究会第7回研究交流会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 硝酸態窒素による根粒の早期老化における LjNLP4/1 の働き2022

    • Author(s)
      藤戸 冴佳; 伊藤 百代; 壽崎 拓哉
    • Organizer
      植物の栄養研究会第7回研究交流会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 硝酸存在下における遺伝子発現の正負の制御を介した根粒形成抑制2021

    • Author(s)
      野崎 翔平; 西田 帆那; 三澤 文香; 伊藤 百代; 鈴木 孝征; 宮川 拓也; 川口 正代司; 田之倉 優; 三浦 謙治; 壽崎 拓哉
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Molecular basis of nitrate-induced control of symbiotic gene expression during nodulation2021

    • Author(s)
      Suzaki Takuya
    • Organizer
      The 14th European Nitrogen Fixation Conference
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] マメ科植物の窒素栄養環境適応機構2021

    • Author(s)
      壽崎 拓哉
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] ミヤコグサにおけるENOD40ノックアウト個体の作出2021

    • Author(s)
      岩本 輝明; 壽崎 拓哉
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 硝酸による根粒共生抑制と炭素利用の関与2021

    • Author(s)
      渡部 将弘; 西田 帆那; 伊藤 百代; 壽崎 拓哉
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 根粒形成を介した窒素栄養獲得制御の頑健性と柔軟性2020

    • Author(s)
      壽崎 拓哉
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] ミヤコグサの MIR2111-5は地上部からの根粒形成の長距離フィードバック制御に必須である2020

    • Author(s)
      大熊直生; 征矢野敬; 壽崎 拓哉; 川口正代司
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 窒素と炭素栄養が根粒形成に及ぼす影響の解析2020

    • Author(s)
      渡部将弘; 伊藤百代; 壽崎 拓哉
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 根粒共生におけるオーキシンメチル化の役割2020

    • Author(s)
      後藤崇支; 征矢野敬; Meng Liu; 壽崎 拓哉; 川口正代司
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 窒素栄養環境下における 植物-根粒菌共生の制御機構2020

    • Author(s)
      壽崎 拓哉
    • Organizer
      筑波大学遺伝子実験センター 形質転換植物デザイン研究拠点 第58回研究セミナー
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 窒素栄養に応答した根粒形成遺伝子の発現制御2020

    • Author(s)
      壽崎 拓哉
    • Organizer
      令和2年度植物科学4拠点アライアンス交流会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 硝酸に応答した根粒共生の抑制と硝酸の取り込みを制御するミヤコグサ NRSYM3 の解析2020

    • Author(s)
      三澤文香;西田帆那;鈴木孝征;伊藤百代;野元美佳;多田安臣;川勝泰二;川口正代司;寿崎拓哉
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ミヤコグサ NLP 転写因子は硝酸に応答して根粒形成初期過程を制御する2020

    • Author(s)
      西田帆那;鈴木孝征;伊藤百代;野元美佳;西嶋遼;川勝泰二;多田安臣;川口正代司;寿崎拓哉
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] A peptide hormone signaling in nitrate-induced control of nodulation2019

    • Author(s)
      Takuya Suzaki
    • Organizer
      International Symposium: Principles of pluripotent stem cells underlying plant vitality
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 奨励賞受賞講演:根粒形成を正および負に制御する分子機構の解析2019

    • Author(s)
      寿崎拓哉
    • Organizer
      日本植物学会第 83 回大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] マメ科植物の窒素栄養応答の遺伝学2019

    • Author(s)
      寿崎拓哉
    • Organizer
      日本遺伝学会第91回大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ミヤコグサNLP転写因子による硝酸に応答した根粒共生抑制制御機構の解析2019

    • Author(s)
      西田帆那;野元美佳;伊藤百代;鈴木孝征;川勝泰二;西嶋遼;多田安臣;川口正代司;寿崎拓哉
    • Organizer
      日本育種学会 第136回講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 硝酸による根粒共生初期過程の抑制制御におけるミヤコグサ NLP 遺伝子の働き2019

    • Author(s)
      西田帆那;野元美佳;伊藤百代;鈴木孝征;川勝泰二;西嶋遼;多田安臣;川口正代司;寿崎拓哉
    • Organizer
      日本植物学会第 83 回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ミヤコグサ NRSYM3 は硝酸に応答した根粒共生の抑制と硝酸の取り込みを制御する2019

    • Author(s)
      三澤文香;西田帆那;鈴木孝征;伊藤百代;野元美佳;多田安臣;川口正代司;寿崎拓哉
    • Organizer
      植物微生物研究会 第 29 回研究交流会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Underlying mechanisms of nitrate-induced control of nodulation2019

    • Author(s)
      Takuya Suzaki
    • Organizer
      International symposium on plant stress and nitrogen fixation 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular link between nitrate inhibition of nodulation and autoregulation of nodulation2019

    • Author(s)
      Takuya Suzaki
    • Organizer
      21st International Congress on Nitrogen Fixation
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 窒素栄養環境下におけるマメ科植物-根粒菌共生の制御2019

    • Author(s)
      寿崎拓哉
    • Organizer
      第24回東京大学生物生産工学研究センターシンポジウム "植物-微生物相互作用 -農業生産性の視点から-”
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Lotus japonicus NRSYM3 is required for nitrate-induced control of nodulation2019

    • Author(s)
      Misawa, Fumika;Nishida, Hanna;Suzuki, Takamasa;Ito, Momoyo;Kawaguchi, Masayoshi;Suzaki, Takuya
    • Organizer
      Frontiers in plant environmental response research: local signaling, long-distance communication and memory for developmental plasticity
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research

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Published: 2019-04-18   Modified: 2024-01-30  

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