| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2023: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2022: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2021: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2020: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2019: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Outline of Final Research Achievements |
The aim of the project was to characterize enzymes involved in biosynthesis of plant cell wall pectin. In this study, glycosyltransferases and acetyltransferases involved in pectin biosynthesis were characterized. The galacturonosyltransferases involved in the synthesis of the pectic rhamnogalacturonan I (RG-I) were found to have eight genes in the Arabidopsis genome. The galactoryltransferase involved in synthesis of RG-I side chain was detected for the first time and given the enzyme number EC 2.4.1.375. The enzymatic activity of RG-I: acetyltransferase was detected for the first time in TBL7. In addition, a substrate for RG-II: Kdo transferase, CMP-Kdo, was prepared.
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