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Genetic basis of speciation process driven by adaptation to new oviposition substrate

Research Project

Project/Area Number 19H03276
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 45020:Evolutionary biology-related
Research InstitutionTokyo Metropolitan University

Principal Investigator

Takahashi Aya  東京都立大学, 理学研究科, 准教授 (90370121)

Co-Investigator(Kenkyū-buntansha) 上村 佳孝  慶應義塾大学, 商学部(日吉), 准教授 (50366952)
野澤 昌文  東京都立大学, 理学研究科, 准教授 (50623534)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2021: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2020: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2019: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Keywords種分化 / 交尾器 / 外部生殖器 / ショウジョウバエ / 遺伝子マッピング / ゲノムシークエンス / 生殖的隔離 / 産卵 / 生殖器
Outline of Research at the Start

環境への適応が引き金となって種が多様化するという考え方は、ダーウィンフィンチの例にあるように広く受け入れられている。しかし、環境へ適応するために進化した形質が生殖的隔離を直接引き起こした例は非常に少ない。これまでの研究により、多くの近縁種が成熟・発酵した果実に産卵する中で、未熟な固い果実に産卵するという新たな形質を獲得したオウトウショウジョウバエの産卵管の特異な形状が、同胞種との交尾の障壁になっていることがわかった。そこで本研究では、この産卵管や交尾の成功に影響する雄の交尾器の形態進化についてその遺伝的背景を明らかにし、生態学的種分化の過程で自然選択の標的となった遺伝的変異の実態を解明する。

Outline of Final Research Achievements

Not like most other Drosophila species, Drosophila suzukii lays its eggs into firm ripening fruits. Its ovipositor is elongated and have many bristles lined up on the edge to pierce the skin of those fruits. This morphological evolution affects not only egg-laying, but also the spatial configurations of male and female genitalia during copulation resulting in the reproductive isolation caused by the mechanical mismatch during interspecific mating. The morphology involved in such form of reproductive isolation between D. suzukii and its closely related species D. subpulchrella was investigated in detail. The genetic analyses of those morphological trait revealed that the DNA sequence regions with frequent structural changes were involved in such morphological evolution. Furthermore, strong candidate genes that may have been responsible for the genetic changes occurred between the two species were also identified.

Academic Significance and Societal Importance of the Research Achievements

本研究では、オウトウショウジョウバエ(Drosophila suzukii)とその近縁種D. subpulchrellaを使って、生物の種が分化していく際に種間での交配を妨げるような生殖器形態の進化が、ゲノム中の構造的な変化が激しい領域に起きる突然変異によって進んできたことが明らかとなった。この成果は、生物の進化を理解する上で、種が分化していく仕組みや昆虫などの動物で生殖器の形が速く進化していることの原因を探究していくために重要な情報となる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (35 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (3 results) Journal Article (8 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 7 results,  Open Access: 8 results) Presentation (22 results) (of which Int'l Joint Research: 9 results,  Invited: 3 results) Remarks (2 results)

  • [Int'l Joint Research] University of California, Berkeley/University of Pittsburgh/University of Hawaii, Manoa(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] University of Hawaii at Manoa/University of California, Berkeley/Stanford University(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Hawaii at Manoa/University of California, Berkeley/Stanford University(米国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] The role of the epidermis enhancer element in positive and negative transcriptional regulation of <i>ebony</i> in <i>Drosophila melanogaster</i>2022

    • Author(s)
      Akiyama Noriyoshi, Sato Shoma, Tanaka Kentaro M, Sakai Takaomi, Takahashi Aya
    • Journal Title

      G3 Genes|Genomes|Genetics

      Volume: 12 Issue: 3

    • DOI

      10.1093/g3journal/jkac010

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A standardized nomenclature and atlas of the female terminalia of Drosophila melanogaster2022

    • Author(s)
      Eden McQueen, (他22名、Takahashi Aya 17番目)
    • Journal Title

      Fly

      Volume: 16 Issue: 1 Pages: 128-151

    • DOI

      10.1080/19336934.2022.2058309

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Highly contiguous assemblies of 101 drosophilid genomes2021

    • Author(s)
      Kim Bernard Y, (他38名、Takahashi Aya 32番目)
    • Journal Title

      eLife

      Volume: 10

    • DOI

      10.7554/elife.66405

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Drosophila suzukii avoidance of microbes in oviposition choice2021

    • Author(s)
      Sato Airi, Tanaka Kentaro M. , Yew Joanne Y., Takahashi Aya
    • Journal Title

      R. Soc. open sci

      Volume: 8 Issue: 1 Pages: 201601-201601

    • DOI

      10.1098/rsos.201601

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Analysis of the genetic basis of speciation induced by adaptation to new oviposition substrates2020

    • Author(s)
      Takahashi Aya
    • Journal Title

      Impact

      Volume: 2020 Issue: 6 Pages: 62-64

    • DOI

      10.21820/23987073.2020.6.62

    • Related Report
      2020 Annual Research Report
    • Open Access
  • [Journal Article] Pleiotropic effects of ebony and tan on pigmentation and cuticular hydrocarbon composition in Drosophila melanogaster2019

    • Author(s)
      Massey Jonathan H., Akiyama Noriyoshi, Bien Tanja, Dreisewerd Klaus, Wittkopp Patricia J., Yew Joanne Y., Takahashi Aya
    • Journal Title

      Frontiers in Physiology

      Volume: 10 Pages: 518-518

    • DOI

      10.3389/fphys.2019.00518

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Starvation tolerance associated with prolonged sleep bouts upon starvation in a single natural population of Drosophila melanogaster2019

    • Author(s)
      Miura Mai, Takahashi Aya
    • Journal Title

      Journal of Evolutionary Biology

      Volume: 32 Issue: 10 Pages: 1117-1123

    • DOI

      10.1111/jeb.13514

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetics of speciation: Advantages and limitations of using <i>Drosophila melanogaster</i> as a model organism2019

    • Author(s)
      髙橋 文、田中 健太郎
    • Journal Title

      JAPANESE JOURNAL OF ECOLOGY

      Volume: 69 Issue: 3 Pages: 183-190

    • DOI

      10.18960/seitai.69.3_183

    • NAID

      130007771841

    • ISSN
      0021-5007, 2424-127X
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Mechanical incompatibility and reproductive isolation between a pair of fruit-damaging Drosophila species.2021

    • Author(s)
      Takahashi Aya
    • Organizer
      The 2nd AsiaEvo Conference 2021 (online)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Variation in ovarian diapause phenotype within a natural population of Drosophila suzukii.2021

    • Author(s)
      Fujichiika Takako, Asano Tsunaki, Takahashi Aya
    • Organizer
      The 2nd AsiaEvo Conference 2021 (online)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rapid evolution of ovipositor scale2021

    • Author(s)
      Tanaka Kentaro M, Takahashi Kanoko, Kamimura Yoshitaka, Takahashi Aya
    • Organizer
      4th Drosophila Genitalia Meeting (online)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Genital structures involved in mechanical incompatibility between Drosophila suzukii and D. subpulchrella.2021

    • Author(s)
      Muto Leona, Sakai Kyoka, Kumagai Tatsuyuki, Kamimura Yoshitaka, Tanaka Kentaro M, Takahashi Aya
    • Organizer
      4th Drosophila Genitalia Meeting (online)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ゲノム編集を用いたebony遺伝子シス発現制御因子の解析2021

    • Author(s)
      秋山礼良,佐藤翔馬,田中健太郎,坂井貴臣,高橋文
    • Organizer
      日本遺伝学会第93回大会(オンライン)
    • Related Report
      2021 Annual Research Report
  • [Presentation] オウトウショウジョウバエを用いた生殖休眠誘導時に発現変動する遺伝子の解析2021

    • Author(s)
      藤近敬子,田中健太郎,高橋文
    • Organizer
      日本遺伝学会第93回大会(オンライン)
    • Related Report
      2021 Annual Research Report
  • [Presentation] Drosophila suzukii avoid commensal acetic acid bacteria in oviposition choice.2021

    • Author(s)
      Sato Airi, Tanaka Kentaro M, Yew Joanne Y, Takahashi Aya
    • Organizer
      62nd Annual Drosophila Research Conference
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] オウトウショウジョウバエの産卵基質選好性:基質上の微生物と硬さの影響2020

    • Author(s)
      佐藤愛莉,田中健太郎,Yew Joanne Y,高橋文
    • Organizer
      日本進化学会第22回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ショウジョウバエの生殖休眠の進化2020

    • Author(s)
      藤近敬子,田中健太郎,高橋文
    • Organizer
      日本進化学会第22回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 産卵基質上の微生物と産卵基質の硬度がオウトウショウジョウバエの産卵基質選好性に与える影響2020

    • Author(s)
      佐藤愛莉,田中健太郎,Yew Joanne Y,高橋文
    • Organizer
      日本動物学会第91回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] オウトウショウジョウバエの産卵基質選択時における微生物の忌避に関わる遺伝子の探索2020

    • Author(s)
      佐藤愛莉,田中健太郎,Yew Joanne Y,高橋文
    • Organizer
      日本遺伝学会第92回大会(要旨集出版による発表)
    • Related Report
      2020 Annual Research Report
  • [Presentation] オウトウショウジョウバエの生殖休眠に関与する遺伝子の解析2020

    • Author(s)
      藤近敬子,田中健太郎,高橋文
    • Organizer
      日本遺伝学会第92回大会(要旨集出版による発表)
    • Related Report
      2020 Annual Research Report
  • [Presentation] Ecologically triggered mechanical isolation between Drosophila suzukii and its sibling species.2020

    • Author(s)
      Takahashi Aya
    • Organizer
      The 5th Asia Pacific Drosophila Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Influence of microbial deposits from other individuals on oviposition site preference in Drosophila suzukii.2020

    • Author(s)
      Sato Airi, Yew Joanne Y, Takahashi Aya
    • Organizer
      The 5th Asia Pacific Drosophila Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Reproductive diapause in Drosophila suzukii.2020

    • Author(s)
      Fujichika Takako, Takahashi Aya
    • Organizer
      The 5th Asia Pacific Drosophila Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Reproductive diapause in Drosophila suzukii.2019

    • Author(s)
      Fujichika Takako, Takahashi Aya
    • Organizer
      International Symposium on Evolutionary Genomics and Bioinformatics 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Influence of signals from other Drosophila individuals on oviposition site preferences of Drosophila suzukii.2019

    • Author(s)
      Sato Airi, Yew Joanne Y, Takahashi Aya
    • Organizer
      日本進化学会第21回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Ovarian diapause in Drosophila suzukii.2019

    • Author(s)
      Fujichika Takako, Takahashi Aya
    • Organizer
      日本進化学会第21回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] CRISPR-Cas9システムによるシス発現調節機構の解析2019

    • Author(s)
      秋山礼良,高橋文
    • Organizer
      日本進化学会第21回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] オウトウショウジョウバエにおける産卵管形態進化の遺伝的基盤の解明2019

    • Author(s)
      高橋佳乃子, 上村佳孝, 田中健太郎, 高橋文
    • Organizer
      日本進化学会第21回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] オウトウショウジョウバエの特異な産卵機能に関する遺伝的基盤の解明2019

    • Author(s)
      高橋佳乃子, 上村佳孝, 田中健太郎, 高橋文
    • Organizer
      日本遺伝学会第91回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] キイロショウジョウバエにおける飢餓時の卵巣発達抑制機構に関わる遺伝子の解析2019

    • Author(s)
      藤近敬子、扇山魁人、高橋文
    • Organizer
      日本遺伝学会第91回大会
    • Related Report
      2019 Annual Research Report
  • [Remarks] Aya Takahashi HP

    • URL

      https://sites.google.com/view/aya-takahashi

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report 2019 Annual Research Report
  • [Remarks] 東京都立大学 理学研究科 生命科学専攻 進化遺伝学研究室

    • URL

      https://www.biol.se.tmu.ac.jp/labo.asp?ID=evogen

    • Related Report
      2021 Annual Research Report

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Published: 2019-04-18   Modified: 2023-01-30  

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