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Effects of wing and body colors on avoidance of interspecific hybridization and speciation in Odonata

Research Project

Project/Area Number 19H03287
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 45030:Biodiversity and systematics-related
Research InstitutionTokyo Metropolitan University

Principal Investigator

Hayashi Fumio  東京都立大学, 理学研究科, 教授 (40212154)

Co-Investigator(Kenkyū-buntansha) 二橋 亮  国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (50549889)
土畑 重人  東京大学, 大学院総合文化研究科, 准教授 (50714995)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2022: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2021: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2020: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywords交尾行動 / 形質置換 / 種分化 / 種内変異 / 種間変異 / 色彩変異 / 交雑 / 種間交雑 / 進化 / 性選択 / 色覚 / 形質痴漢
Outline of Research at the Start

トンボの成虫は基本的に視覚で相手を認識することから、体色や斑紋に著しい多様性が見られる。しかし、野外において種間交雑が起こることがある。種間交雑に伴う行動や形態の形質置換に関しては、生殖隔離の強度や近縁種間で体色が多様化した分子基盤は全く解明されていないのが現状である。そこで、本研究は、人為的交雑実験や集団遺伝学、遺伝子機能解析等の実験系を用いて、種間交雑を回避する生殖隔離の強度に地域差は見られるのか、近縁種間(あるいは近縁種の同所的集団と異所的集団間)で体色や色覚の分子基盤にどのような差異が生じているのか、という未解明の問いに取り組む。

Outline of Final Research Achievements

Speciation in insects includes morphological, physiological, and behavioral processes which differ among insect groups. In damselflies and dragonflies (Odonata), they fly in the daytime and use color visions in mating, resulting in diverse color patterns in wing and body marking patterns among species and also within the species. However, interspecific hybridization is suggested to occur in Odonata in the field. In such a situation of hybridization, the following three cases are theoretically predicted. First, the two closely related species become a single genetically mixed species by hybridization; second, character displacement occurs to avoid hybridization; and third, tow closely related species are maintained by already acquired differences not to mate with the opposite species. In this project, we examined how these three cases are common in Odonata using morphological comparisons and genetic analyses.

Academic Significance and Societal Importance of the Research Achievements

本研究の最大の特色は、体色や色覚に著しい多様性が見られるトンボ類について、形態だけでなく遺伝学的にもその機構を解明した点にある。特に、カワトンボ属では国内で種間の形態差が段階的に変化することに加えて、過去に交雑が生じていたことが申請者らの研究により明らかになってきたが、さらにそれを分布域全体で詳細に比較することによって、近縁種間の交雑に伴う形質置換だけでなく、雑種崩壊や完全な種分化を含む複雑な遺伝的・形態的構造をもつ集団からなることを明らかにした。つまり、昆虫類の体色が種分化に果たす役割は一律ではなく、流動的な生殖的隔離を内包することを明らかにした点で独創性の高い研究であると思われる。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (21 results)

All 2022 2021 2020 Other

All Int'l Joint Research (2 results) Journal Article (17 results) (of which Peer Reviewed: 16 results,  Open Access: 4 results) Presentation (2 results) (of which Invited: 2 results)

  • [Int'l Joint Research] China Agricultural University(中国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] 中国農業大学(中国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] 富山県からオゼイトトンボを初記録2022

    • Author(s)
      中田達哉・澤田研太・新堀修・二橋亮
    • Journal Title

      Tombo

      Volume: 65 Pages: 75-77

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 埼玉県におけるカワトンボ属の分布調査2022

    • Author(s)
      深谷航・二橋亮
    • Journal Title

      Tombo

      Volume: 65 Pages: 58-62

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] キトンボの淡翅色個体の記録2022

    • Author(s)
      中田達哉・二橋亮・堀繁久
    • Journal Title

      Tombo

      Volume: 65 Pages: 47-48

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 国内2 例目のオオイトトンボとムスジイトトンボの種間雑種を茨城県石岡市で採集2022

    • Author(s)
      森田倫太郎・渡邊貴弘・二橋亮
    • Journal Title

      Tombo

      Volume: 65 Pages: 44-46

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] シオカラトンボ属の黒化異常個体2022

    • Author(s)
      二橋亮
    • Journal Title

      Tombo

      Volume: 64 Pages: 27-32

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 岡山県から発見されたアサヒナカワトンボの褐色翅個体2022

    • Author(s)
      脇本浩・守安敦・二橋亮
    • Journal Title

      Tombo

      Volume: 64 Pages: 25-26

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 神奈川県におけるアサヒナカワトンボ♂の褐色翅個体の記録2022

    • Author(s)
      川島逸郎・二橋亮
    • Journal Title

      Tombo

      Volume: 64 Pages: 24-24

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 栃木県で得られたニホンカワトンボの橙色翅型と無色翅型の中間型2022

    • Author(s)
      深谷航・二橋亮
    • Journal Title

      Tombo

      Volume: 64 Pages: 21-23

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanisms underlying metamorphosis in the most-ancestral winged insect2022

    • Author(s)
      Okuda G, Moriyama M, Kawahara-Miki R, Yajima S, Fukatsu, T, Futahashi R
    • Journal Title

      PNAS

      Volume: 119 Pages: 1-12

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comprehensive comparative morphology and developmental staging of final instar larvae toward metamorphosis in the insect order Odonata2021

    • Author(s)
      Okude Genta、Fukatsu Takema、Futahashi Ryo
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 5164-5164

    • DOI

      10.1038/s41598-021-84639-2

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Electroporation-mediated RNA Interference Method in Odonata2021

    • Author(s)
      Okude Genta、Fukatsu Takema、Futahashi Ryo
    • Journal Title

      Journal of Visualized Experiments

      Volume: 168 Issue: 168 Pages: 1-20

    • DOI

      10.3791/61952

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pigmentation and color pattern diversity in Odonata2021

    • Author(s)
      Okude Genta、Futahashi Ryo
    • Journal Title

      Current Opinion in Genetics & Development

      Volume: 69 Pages: 14-20

    • DOI

      10.1016/j.gde.2020.12.014

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 札幌市で記録されたタイリクアキアカネの検証.2021

    • Author(s)
      横山透・二橋亮
    • Journal Title

      北海道トンボ研究会報

      Volume: 32 Pages: 11-12

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Diversity of UV Reflection Patterns in Odonata2020

    • Author(s)
      Futahashi Ryo
    • Journal Title

      Frontiers in Ecology and Evolution

      Volume: 8 Pages: 201-201

    • DOI

      10.3389/fevo.2020.00201

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Interspecific crossing between blue-tailed damselflies Ischnura elegans and I. senegalensis in the laboratory.2020

    • Author(s)
      Okude G., Fukatsu T., Futahashi R.
    • Journal Title

      Entomological Science

      Volume: 23 Issue: 2 Pages: 165-172

    • DOI

      10.1111/ens.12408

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interspecific hybrid between Paracercion sieboldii and P. melanotum from Japan (Odonata: Coenagrionidae).2020

    • Author(s)
      Okude G., Futahashi R.
    • Journal Title

      Notulae odonatologicae

      Volume: 9 Pages: 204-208

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 高知県でマイコアカネとマユタテアカネの種間雑種を採集.2020

    • Author(s)
      杉村光俊・二橋亮
    • Journal Title

      Tombo

      Volume: 62 Pages: 104-105

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] カワトンボ属2種の翅の複雑な色彩多型を交雑から読み解く2022

    • Author(s)
      林 文男
    • Organizer
      日本昆虫学会第82回大会シンポジウム「DNAから紐解く昆虫の不思議な世界―繁殖生殖の進化―」
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] トンボの不思議を追いかけて2022

    • Author(s)
      二橋亮
    • Organizer
      日本動物学会第93回大会本部企画シンポジウム
    • Related Report
      2022 Annual Research Report
    • Invited

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Published: 2019-04-18   Modified: 2024-01-30  

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