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Genetic differentiation of plants between upper and lower elevations and the role of pollinating insects in maintaining differentiation

Research Project

Project/Area Number 19H03300
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 45040:Ecology and environment-related
Research InstitutionShinshu University

Principal Investigator

Itino Takao  信州大学, 学術研究院理学系, 教授 (20176291)

Co-Investigator(Kenkyū-buntansha) 陶山 佳久  東北大学, 農学研究科, 教授 (60282315)
Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2023: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2022: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2021: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Keywords標高種分化 / 草本植物 / 送粉昆虫 / 遺伝分化 / 生態型 / MIG-seq解析 / 適応進化 / 生物間相互作用
Outline of Research at the Start

申請者らは最近、山地性植物サラシナショウマにおいて、標高上下間で遺伝的分化が起こっており、遺伝的に異なる3つの生態型の間で花形質や送粉者が異なっていることを見いだした。本研究ではこの知見をふまえ、広い標高域にまたがって分布する約50種の植物種について、まず標高上下間での遺伝的分化の有無を確認し、さらに分化の維持メカニズムを追究する。本研究によって、標高上下間での遺伝的な分化が広く示されれば、従来よりも細かい保全単位で山地性植物を保全すべきであるといった政策の見直しに発展させることが可能となり、また標高上下での側所的な種分化が明らかになれば「標高種分化」の初めての実証例となる。

Outline of Final Research Achievements

We tested whether adaptive differentiation between upper and lower elevations leads to genetic differentiation in four bumblebee-pollinated herbaceous species distributed in the Japanese Alps.
In Prunella vulgaris, Lamium album and Aquilegia buergeriana, flower size corresponded to inter-population variation in pollinator size due to differences in the main pollinators at different elevations. No genetic differentiation between upper and lower elevations was detected in these three species, and genetic differentiation was detected among mountain ranges.
In Impatiens noli-tangere, the "early-flowering type" and the "late-flowering type," in which the flowering time and other morphological traits were clearly differentiated between the upper and lower elevations in a narrow range, were parapatrically distributed, and the two types were genetically differentiated. In I. noli-tangere, adaptive differentiation to altitude was associated with genetic differentiation.

Academic Significance and Societal Importance of the Research Achievements

標高に伴う側所的な2型の分化が本研究成果により示された。繁殖時期(花期)の違いが2型の側所分化を維持する機構として重要であることも示され、標高間種分化という未開拓の研究分野にとって嚆矢とも見なすべき成果が得られたといえる。この標高間分化の発見は、従来よりも細かい保全単位で山地性植物を保全すべきであるといった政策の見直しにつながりうる基礎情報となる。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (26 results)

All 2023 2022 2021 2020 2019

All Journal Article (13 results) (of which Peer Reviewed: 12 results,  Open Access: 2 results) Presentation (13 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Sympatric co-existence of two ecotypes of Impatiens noli-tangere (Balsaminaceae) with different morphology and flowering phenology2023

    • Author(s)
      Toji Tsubasa、Yamamoto Tetsuya、Kondo Hikaru、Shinohara Yoshinori、Itino Takao
    • Journal Title

      Journal of Plant Research

      Volume: 136 Issue: 3 Pages: 323-331

    • DOI

      10.1007/s10265-023-01444-w

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measurement of inbreeding depression in Rhododendron kaempferi: seed production, germination, juvenile survival, and growth.2022

    • Author(s)
      Takahashi K and Itino T
    • Journal Title

      Botany

      Volume: 100 Issue: 4 Pages: 359-365

    • DOI

      10.1139/cjb-2021-0038

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intraspecific independent evolution of floral spur length in response to local flower visitor size in Japanese <i>Aquilegia</i> in different mountain regions2022

    • Author(s)
      Toji Tsubasa、Hirota Shun K.、Ishimoto Natsumi、Suyama Yoshihisa、Itino Takao
    • Journal Title

      Ecology and Evolution

      Volume: 12 Issue: 3

    • DOI

      10.1002/ece3.8668

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intraspecific convergence of floral size correlates with pollinator size on different mountains: a case study of a bumblebee-pollinated Lamium (Lamiaceae) flowers in Japan.2021

    • Author(s)
      Toji T, Ishimoto N, Egawa S, Nakase Y, Hattori M and Itino T
    • Journal Title

      BMC Ecol Evol

      Volume: 21 Issue: 1

    • DOI

      10.1186/s12862-021-01796-8

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of pollinator size on seed set in Lamium album var. barbatum.2021

    • Author(s)
      Hattori M, Tamada Y and Itino T
    • Journal Title

      Plant Ecology and Evolution

      Volume: 154 Issue: 2 Pages: 201-206

    • DOI

      10.5091/plecevo.2021.1642

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differences in the flower visitor behaviour on female and hermaphroditic flowers of <i>Cimicifuga simplex</i>2021

    • Author(s)
      Toji Tsubasa、Itino Takao
    • Journal Title

      Journal of Natural History

      Volume: 55 Issue: 19-20 Pages: 1289-1298

    • DOI

      10.1080/00222933.2021.1946611

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Discovery of cryptic diversity in phytophagous gall midges (Diptera: Cecidomyiidae) associated with different ecotypes of the perennial herb Cimicifuga simplex2021

    • Author(s)
      Toji Tsubasa、Tokuda Makoto、Yamamoto Tetsuya、Nakase Yuta、Itino Takao
    • Journal Title

      Journal of Asia-Pacific Entomology

      Volume: 24 Issue: 4 Pages: 1010-1016

    • DOI

      10.1016/j.aspen.2021.09.006

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 日本の中部山岳域におけるナギナタコウジュの分子系統:高山生態型の発見とユーラシア大陸産ナギナタコウジュとの遺伝的差異.2021

    • Author(s)
      田路翼, 市野隆雄
    • Journal Title

      信州大学環境科学年報

      Volume: 43 Pages: 28-36

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Differences in sex expression and mating systems in three pollination morphs of Cimicifuga simplex2020

    • Author(s)
      Toji Tsubasa、Itino Takao
    • Journal Title

      Plant Species Biology

      Volume: 35 Issue: 2 Pages: 112-119

    • DOI

      10.1111/1442-1984.12265

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Geographic changes in pollinator species composition affect the corolla tube length of self‐heal (Prunella vulgaris L.): Evidence from three elevational gradients2020

    • Author(s)
      Egawa Shin、Hirose Koyo、Itino Takao
    • Journal Title

      Ecological Research

      Volume: 35 Issue: 5 Pages: 819-825

    • DOI

      10.1111/1440-1703.12146

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of flower-visiting behaviour of bumblebees and swallowtail butterflies to the Japanese azalea (Rhododendron japonicum)2020

    • Author(s)
      Takahashi K and Itino T
    • Journal Title

      Journal of Pollination Ecology

      Volume: 26 Pages: 32-37

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal change of flower sex ratio and pollinator dynamics in three reproductive ecotypes of protandrous plant2020

    • Author(s)
      Toji Tsubasa、Ishimoto Natsumi、Itino Takao
    • Journal Title

      Ecosphere

      Volume: 11 Issue: 9

    • DOI

      10.1002/ecs2.3251

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 上高地におけるマルハナバチ属の体サイズの種間変異および標高間の種内変異2020

    • Author(s)
      江川信・市野隆雄
    • Journal Title

      New Entomologist

      Volume: 69 Pages: 39-47

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Presentation] 長野県安曇野市一山域で見られたキツリフネの生態型分化2023

    • Author(s)
      近藤輝留、田路翼、廣田峻、陶山佳久、市野隆雄
    • Organizer
      第55回種生物学会シンポジウム
    • Related Report
      2023 Annual Research Report
  • [Presentation] キツリフネの開花時期の分化を伴った局所適応:相互移植実験による検証.2022

    • Author(s)
      近藤輝留, 田路翼, 中瀬悠太, 市野隆雄
    • Organizer
      第54回種生物学会シンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] キツリフネのエコタイプ間での開花時期の分化:相互移植実験による検証2022

    • Author(s)
      近藤輝留, 田路翼, 中瀬悠太, 市野隆雄
    • Organizer
      2022年度日本生態学会中部地区大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] マルハナバチ属昆虫が駆動する花のサイズの山域間独立進化 -4種植物における共通点2022

    • Author(s)
      田路翼, 廣田峻, 石本夏海, 中瀬悠太, 江川信, 中村駿介, 服部充, 陶山佳久, 市野隆雄
    • Organizer
      日本昆虫学会第82回大会,
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 相互移植実験によって明らかになったキツリフネのエコタイプ間での形質分化2022

    • Author(s)
      近藤輝留, 田路翼, 中瀬悠太, 江川信, 塩見温士, 山本哲也, 市野隆雄
    • Organizer
      第70回日本生態学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 湿潤な沢と乾燥した尾根間の相互移植実験によって明らかになったキツリフネの局所適応.2021

    • Author(s)
      近藤輝留, 江川信, 塩見温士, 山本哲也, 田路翼, 中瀬悠太, 市野隆雄
    • Organizer
      第69回日本生態学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 一集団内において数年間維持されている花サイズの二峰性: 大小2種の送粉者への適応分化2021

    • Author(s)
      田路翼, 石本夏海, 江川信, 中瀬悠太, 服部充, 市野隆雄
    • Organizer
      第37回 個体群生態学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 送粉者サイズに応じた花サイズの山域間独立進化: 4種における実証2021

    • Author(s)
      田路翼, 廣田峻, 石本夏海, 中瀬悠太, 江川信, 中村駿介, 服部充, 陶山佳久, 市野隆雄
    • Organizer
      第53回 種生物学シンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] Bimodal flower size distribution caused by disruptive selection through pollinator flower size preferences2021

    • Author(s)
      Tsubasa Toji, Natsumi Ishimoto, Yuta Nakase, Shin Egawa, Mitsuru Hattori, Takao Itino
    • Organizer
      第69回 日本生態学会大会
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The effect of pollinator quality and quantity on sex ratio and self-breeding of three ecotypes of Cimicifuga simplex2020

    • Author(s)
      Toji T, Ishimoto N, Itino T
    • Organizer
      第67回日本生態学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] オドリコソウの花サイズ変異は送粉者相と送粉者体サイズの地理的変異に影響される2020

    • Author(s)
      石本夏海・田路翼・江川信・中瀬悠太・市野隆雄
    • Organizer
      第67回日本生態学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] オドリコソウの集団内・集団間における花筒長の変異と訪花者サイズの変異の関係2019

    • Author(s)
      田路翼・石本夏海・江川信・中瀬悠太・市野隆雄
    • Organizer
      日本生態学会中部地区大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 一集団内における送粉者の行動の違いがもたらす花サイズの二峰性-オドリコソウを例に2019

    • Author(s)
      田路翼・石本夏海・江川信・中瀬悠太・市野隆雄
    • Organizer
      第51回種生物学シンポジウム
    • Related Report
      2019 Annual Research Report

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Published: 2019-04-18   Modified: 2025-01-30  

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