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Phsyiological study in developmental synaptic competition and elimination

Research Project

Project/Area Number 19H03343
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 46030:Function of nervous system-related
Research InstitutionTokyo Women's Medical University

Principal Investigator

Miyata Mariko  東京女子医科大学, 医学部, 教授 (70281631)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2021: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Keywords視床 / シナプス刈り込み / プレシナプス / シナプス刈込み / 感覚経験依存的 / 神経伝達物質放出機能 / 発達 / 内側毛帯シナプス / シナプス刈込 / シナプス前機能 / シナプス競合 / 開口放出
Outline of Research at the Start

発達期の脳では、発達初期に冗長で未熟なシナプス結合を作った後、経験によって必要なものが強められて残存し、不要なものが弱められ(シナプス競合)やがては除去される(シナプス刈り込み)過程を経て、精巧で成熟した神経回路が完成する。シナプス刈り込みの研究は、機能的解析を必要とするシナプス競合のメカニズムは依然として不明な点が多い。とりわけ、刈り込まれる対象である軸索のシナプス前部(プレシナプス)の機能は未だ皆無である。そこで本研究は、経験依存的なシナプス刈り込み現象を定量的に解析できる視床ニューロンの感覚線維シナプスに着目し、発達過程におけるシナプス前部後部両者の機能変化をシナプス選択的に解明する。

Outline of Final Research Achievements

For the maturation of brain neuronal circuit, surviving of necessary synapses and elimination of redundant synapses are crucial, but functional differentiations during the process remain elusive. Here, we found distinct developments of transmitter release kinetics at surviving and eliminating presynaptic terminals. At surviving terminals, total number of releasable vesicles enlarges first, and capacity for rapid exocytosis is established experience-dependently thereafter. At eliminating terminals, not only transmitter release mechanism, but also calcium current and terminal size exhibit no developmental maturations. By contrast, developmental changes of action potential waveforms are indistinguishable between surviving or eliminating terminals, and showed no experience-independence. Thus, we reveal pathway-specific, experience-dependent, and experience-independent developmental presynaptic maturation, which will lead to deeper understandings of brain neuronal circuit establishment.

Academic Significance and Societal Importance of the Research Achievements

今まで、発達期シナプス刈り込みにおいては、生存するシナプスと刈り込まれるシナプスが存在するが、これらのシナプス小胞の放出能に関しては、未知であった。今回、中枢神経系において世界ではじめてこれらを明らかにし、PNASに発表した。

Report

(3 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • Research Products

    (32 results)

All 2022 2021 2020 2019

All Journal Article (13 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 12 results,  Open Access: 7 results) Presentation (19 results) (of which Int'l Joint Research: 6 results,  Invited: 5 results)

  • [Journal Article] Dysfunction of parvalbumin-expressing cells in the thalamic reticular nucleus induces cortical spike-and-wave discharges and an unconscious state2022

    • Author(s)
      Abdelaal Manal S.、Midorikawa Mitsuharu、Suzuki Toru、Kobayashi Kenta、Takata Norio、Miyata Mariko、Mimura Masaru、Tanaka Kenji F.
    • Journal Title

      Brain Communications

      Volume: 4 Issue: 2 Pages: 1-17

    • DOI

      10.1093/braincomms/fcac010

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 感情クオリア構造とその神経基盤の解明に向けて.2022

    • Author(s)
      土谷尚嗣 宮田麻理子
    • Journal Title

      生体の科学

      Volume: 73 (1) Pages: 64-69

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Facial nerve regeneration with bioabsorbable collagen conduits filled with collagen filaments: an experimental study2021

    • Author(s)
      Nami Hayakawa, Hajime Matsumine, Kaori Fujii, Hironobu Osaki, Yoshifumi Ueta, Wataru Kamei, Yosuke Niimi, Mariko Miyata, Hiroyuki Sakurai
    • Journal Title

      Regenerative Therapy

      Volume: 18 Pages: 302-308

    • DOI

      10.1016/j.reth.2021.08.006

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] FoxG1 regulates the formation of cortical GABAergic circuit during an early postnatal critical period resulting in autism spectrum disorder-like phenotypes2021

    • Author(s)
      Miyoshi Goichi、Ueta Yoshifumi、Natsubori Akiyo、Hiraga Kou、Osaki Hironobu、Yagasaki Yuki、Kishi Yusuke、Yanagawa Yuchio、Fishell Gord、Machold Robert P、Miyata Mariko
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 3773-3773

    • DOI

      10.1038/s41467-021-23987-z

    • NAID

      120007125060

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Electrophysiological and anatomical characterization of synaptic remodeling in the mouse whisker thalamus2021

    • Author(s)
      Ueta Y., Miyata M.
    • Journal Title

      STAR Protocols

      Volume: 2 Issue: 3 Pages: 100743-100743

    • DOI

      10.1016/j.xpro.2021.100743

    • NAID

      120007141578

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Distinct nociresponsive region in mouse primary somatosensory cortex2021

    • Author(s)
      Hironobu Osaki, Moeko Kanaya, Yoshifumi Ueta, Mariko Miyata
    • Journal Title

      bioRxiv

      Volume: ePub

    • DOI

      10.1101/2021.04.14.439725

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prevention of denervated muscle atrophy with accelerated nerve-regeneration by babysitter procedure in rat facial nerve paralysis model2020

    • Author(s)
      Hashimoto Kazuki、Matsumine Hajime、Osaki Hironobu、Ueta Yoshifumi、Kamei Wataru、Shimizu Mari、Fujii Kaori、Niimi Yosuke、Miyata Mariko、Sakurai Hiroyuki
    • Journal Title

      Microsurgery

      Volume: 11 Issue: 1 Pages: 3-3

    • DOI

      10.1002/micr.30580

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Layer-specific sensory processing impairment in the primary somatosensory cortex after motor cortex infarction2020

    • Author(s)
      Fukui A, Osaki H, Ueta Y, Kobayashi K, Muragaki Y, Kawamata T, Miyata M
    • Journal Title

      Sci Rep

      Volume: 10 Issue: 1 Pages: 3771-3771

    • DOI

      10.1038/s41598-020-60662-7

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The metabotropic glutamate receptor subtype 1 regulates development and maintenance of lemniscal synaptic connectivity in the somatosensory thalamus2019

    • Author(s)
      Narushima M, Yagasaki Y, Takeuchi Y, Aiba A, Miyata M
    • Journal Title

      PLoS ONE

      Volume: 14 Issue: 12 Pages: 820-820

    • DOI

      10.1371/journal.pone.0226820

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dedifferentiated fat cells in polyglycolic acid-collagen nerve conduits promote rat facial nerve regeneration.2019

    • Author(s)
      Hiroshi Fujimaki†, Hajime Matsumine*, Hironobu Osaki, Yoshifumi Ueta, Wataru Kamei, Mari Shimizu, Kazuki Hashimoto, Kaori Fujii, Tomohiko Kazama, Taro Matsumoto, Yosuke Niimi, Mariko Miyata, Hiroyuki Sakurai
    • Journal Title

      Regenerative Therapy

      Volume: 11 Pages: 240-248

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ipsi- and contralateral corticocortical projection-dependent subcircuits in layer 2 of the rat frontal cortex2019

    • Author(s)
      Ueta Yoshifumi、Sohn Jaerin、Agahari Fransiscus Adrian、Im Sanghun、Hirai Yasuharu、Miyata Mariko、Kawaguchi Yasuo
    • Journal Title

      Journal of Neurophysiology

      Volume: 122 Issue: 4 Pages: 1461-1472

    • DOI

      10.1152/jn.00333.2019

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Layer-specific sensory processing impairment in the primary somatosensory cortex after motor cortex infarction2019

    • Author(s)
      Fukui Atsushi、Osaki Hironobu、Ueta Yoshifumi、Muragaki Yoshihiro、Kawamata Takakazu、Miyata Mariko
    • Journal Title

      bioRxiv

      Volume: XX

    • DOI

      10.1101/778167

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Utilization of Tablet Device in Elected Lecture (Medical Informatics) of Medical School2019

    • Author(s)
      佐藤 梓、辻野 賢治、加藤 砂織、田部 瑶子、上田 明子、中谷 充、藤原 吉希、宮田 麻理子、尾﨑 眞
    • Journal Title

      Research Report of Informatics Education

      Volume: 1 Issue: 0 Pages: 44-46

    • DOI

      10.24711/rrie.1.0_44

    • NAID

      130007651676

    • ISSN
      2434-3463
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] Social stress-induced remodeling of neuronal circuits in the sensory thalamus.2022

    • Author(s)
      Nakayama Hisako, Miyata Mariko
    • Organizer
      The 99th Annual Meeting of the Physiological Society of Japan
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 視床プレシナプス機能の発達2022

    • Author(s)
      宮田麻理子
    • Organizer
      学術変革領域班会議(脳の若返りによる生涯可塑性誘導-iPlasticity-臨界期機構の解明と操作)
    • Related Report
      2021 Annual Research Report
  • [Presentation] Development of presynaptic functions at surviving and eliminated synapses in the somatosensory thalamus.2021

    • Author(s)
      Midorikawa Mitsuharu, Miyata Mariko
    • Organizer
      第44回 日本神経科学大会 第1回 CJK 国際会議
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Postsynaptic activity dependence of selective strengthening and an establishment of mono-innervation at whisker-related afferents to thalamic neuronal synapses.2021

    • Author(s)
      Nakayama Hisako, Miyata Mariko
    • Organizer
      第44回日本神経科学大会/第1回 CJK 国際会議
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Optogenetic inhibition of the primary somatosensory cortex neurons reduces pain behavior in free-moving mice.2021

    • Author(s)
      Hironobu Osaki, Moeko Kanaya, Miyata Mariko
    • Organizer
      1st International Symposium on Hyper-Adaptability
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 異所痛を生み出す脳内神経回路改編メカニズム2021

    • Author(s)
      宮田麻理子
    • Organizer
      第43回日本疼痛学会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] マウス体性感覚視床における末梢神経損傷後のシナプス改編制御.2021

    • Author(s)
      植田禎史, 宮田麻理子
    • Organizer
      第68回中部日本生理学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 感覚によってつくられる視床の柔らかさ2021

    • Author(s)
      宮田麻理子
    • Organizer
      第44回日本神経科学大会
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Microglia in the brainstem induces synaptic remodeling and map reorganization in the thalamus after peripheral nerve injury.2021

    • Author(s)
      Ueta Yoshifumi, Miyata Mariko
    • Organizer
      The 44th Annual Meeting of the Japan Neuroscience Society
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Area- and layer-specific distribution of nociceptive neurons in the mouse primary somatosensory cortex.2020

    • Author(s)
      Osaki Hironobu, Kanaya Moeko, Ueta Yoshifumi, Miyata Mariko
    • Organizer
      International Workshop on Frontiers in Defensive Survival Circuit Research,
    • Related Report
      2019 Annual Research Report
  • [Presentation] Team-Based Learning(TBL)を用いた生理学教育2020

    • Author(s)
      宮田麻理子
    • Organizer
      第97回日本生理学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Role of the right frontal orienting field in visuospatial attention.2020

    • Author(s)
      Ishii Daisuke, Osaki Hironobu, Yozu Arito, Ishibashi Kiyoshige, Kawamura Kenta, Miyata Mariko, Kohno Yutaka
    • Organizer
      The 97th Annual Meeting of the Physiological Society of Japan,
    • Related Report
      2019 Annual Research Report
  • [Presentation] Microglia mediate peripheral nerve injury-induced plasticity to the thalamus.2020

    • Author(s)
      Ueta Yoshifumi,Sekino Sachie ,Katayama Yoko,Miyata Mariko
    • Organizer
      The 97th Annual Meeting of the Physiological Society of Japan
    • Related Report
      2019 Annual Research Report
  • [Presentation] Direct measurements of transmitter release kinetics at lemniscal ber terminals in the somatosensory thalamus.2020

    • Author(s)
      Midorikawa Mitsuharu, Miyata Mariko
    • Organizer
      The 97th Annual Meeting of the Physiological Society of Japan
    • Related Report
      2019 Annual Research Report
  • [Presentation] Neuronal activities underlying an experience-dependent synaptic remodeling in the developing sensory thalamus.2020

    • Author(s)
      Nakayama Hisako , Miyata Mariko
    • Organizer
      The 97th Annual Meeting of the Physiological Society of Japan
    • Related Report
      2019 Annual Research Report
  • [Presentation] The area- and layer-specific distribution of nociceptive neurons in the primary somatosensory cortex of mice.2019

    • Author(s)
      Osaki Hironobu, Ueta Yoshifumi, Miyata Mariko
    • Organizer
      The 42nd Annual Meeting of the Japan Neuroscience Society,
    • Related Report
      2019 Annual Research Report
  • [Presentation] Dynamic FoxG1 expression levels regulate autism associated behavioral circuit.2019

    • Author(s)
      Miyoshi Goichi, Ueta Yoshifumi, Yagasaki Yuki, Osaki Hironobu, Machold Rob, Fishell Gord, Miyata Mariko
    • Organizer
      The 42nd Annual Meeting of the Japan Neuroscience Society
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Direct measurements of transmitter release from lemniscal fiber terminals in the somatosensory thalamus.2019

    • Author(s)
      Midorikawa Mitsuharu, Miyata Mariko
    • Organizer
      The 42nd Annual Meeting of the Japan Neuroscience Society,
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Microglia regulate thalamic circuit reorganization induced by peripheral nerve injury.2019

    • Author(s)
      Ueta Yoshifumi, Miyata Mariko
    • Organizer
      The 42nd Annual Meeting of the Japan Neuroscience Society,
    • Related Report
      2019 Annual Research Report

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Published: 2019-04-18   Modified: 2023-03-23  

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