| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2021: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2020: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Outline of Final Research Achievements |
This study focused on RNA secondary structure using human somatic cells and human pluripotent stem cells and found that the 5' UTR regions of a specific gene contribute to pluripotent stem cell-specific translational regulation. Furthermore, we also developed a method to analyze the RNA secondary structure of individual RNA splicing variants with high precision. Using this method combined with our original reporter system, we identified splicing variants that show high translation efficiency only in pluripotent stem cells and determined their RNA secondary structures. We also extracted candidate regulators that could bind to the pluripotency-specific RNA secondary structures. This study demonstrates a link between the post-transcriptional RNA regulatory network and the maintenance of pluripotency.
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