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Investigation of virulence mechanism of Gram-positive bacteria regulated by various RNA binding proteins

Research Project

Project/Area Number 19H03466
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 49050:Bacteriology-related
Research InstitutionOkayama University

Principal Investigator

KAITO Chikara  岡山大学, 医歯薬学域, 教授 (60420238)

Co-Investigator(Kenkyū-buntansha) 寺尾 豊  新潟大学, 医歯学系, 教授 (50397717)
古田 和幸  岡山大学, 医歯薬学域, 准教授 (50644936)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2021: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2020: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2019: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Keywords酸化ヌクレオチド / カイコプラズマ / RpoB / リファンピシン / エリスロマイシン / 病原性 / 8-oxo-GTP / 酸化ストレス / 病原性因子 / ヌクレオチドピロフォスファターゼ / RNA相互作用因子 / グラム陽性細菌 / 病原性制御
Outline of Research at the Start

本研究の目的は、細菌が宿主環境を認識し、病原因子を発現制御する過程におけるRNA相互作用因子群の役割を明らかにすることである。本課題では、代表者が確立した細菌の病原性評価系と、RNAとタンパク質の新しい解析技術を連携させ、病原性調節に働く新規RNA相互作用因子を網羅的に同定する。さらに、RNA相互作用因子が引き起こすRNAの質と量の調節機構を解明し、細菌の病原性発現における役割を解明する。

Outline of Final Research Achievements

Staphylococcus aureus and Streptococcus pneumoniae are pathogenic bacteria that cause various diseases. In order to establish new therapeutic strategies against drug-resistant bacteria, it is necessary to understand the mechanisms of pathogenesis of these bacteria. In this study, we analyzed the biochemical properties of a novel nucleotidase and demonstrated that this enzyme degrades oxidized nucleotides, leading to oxidative stress resistance in S. aureus, and functions in the development of pathogenicity. In the course of our research, we also discovered the reaction of aggregation formation by S. aureus in the plasma of silkworms. Analysis using gene-deficient strains suggested that the aggregation formation plays an important role in the development of pathogenicity of S. aureus.

Academic Significance and Societal Importance of the Research Achievements

本研究は,黄色ブドウ球菌の新規ヌクレオチド分解酵素を解析することにより,黄色ブドウ球菌が酸化ヌクレオチドを分解することにより酸化ストレス抵抗性を獲得することを発見した。細菌が多細胞生物への感染を可能とする新たな分子機構を明らかにした点で学術的意義がある。また,本酵素に対する阻害剤は新たな細菌感染症治療薬の創出につながる点で社会的意義がある。さらに,カイコプラズマ中における黄色ブドウ球菌の凝集塊形成の発見は,哺乳類と昆虫という異なる宿主間で保存された黄色ブドウ球菌の病原性発現ストラテジーの一端を明らかにした点で学術的意義がある。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (10 results)

All 2021 2020 2019

All Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 5 results) Presentation (2 results) (of which Invited: 2 results)

  • [Journal Article] Proteolytic cleavage of HLA class II by human neutrophil elastase in pneumococcal pneumonia2021

    • Author(s)
      Hisanori Domon, Tomoki Maekawa, Toshihito Isono, Kazuyuki Furuta, Chikara Kaito, Yutaka Terao
    • Journal Title

      Sci Rep

      Volume: 11 Issue: 1 Pages: 2432-2432

    • DOI

      10.1038/s41598-021-82212-5

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Total Syntheses and Chemical Biology Studies of Hymeglusin and Fusarilactone A, Novel Circumventors of β-Lactam Drug Resistance in Methicillin-Resistant Staphylococcus aureus2021

    • Author(s)
      Kanaida M, Kimishima A, Eguchi S, Iwatsuki M, Watanabe Y, Honsho M, Hirose T, Noguchi Y, Nonaka K, Sennari G, Matsui H, Kaito C, Hanaki H, Asami Y, Sunazuka T.
    • Journal Title

      ChemMedChem.

      Volume: in press. Issue: 13 Pages: 2106-2111

    • DOI

      10.1002/cmdc.202100219

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Animal infection models using non-mammals2020

    • Author(s)
      Chikara Kaito, Kanade Murakami, Lina Imai, Kazuyuki Furuta
    • Journal Title

      Microbiology and immunology

      Volume: 64 Issue: 9 Pages: 585-592

    • DOI

      10.1111/1348-0421.12834

    • NAID

      120006953505

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Epidemiological study on the relationship between toxin production and psm‐mec mutations in MRSA isolates in Thailand2020

    • Author(s)
      Tabuchi Fumiaki、Lulitanond Aroonlug、Lulitanond Viraphong、Thunyaharn Sudaluck、Kaito Chikara
    • Journal Title

      Microbiology and Immunology

      Volume: 64 Issue: 3 Pages: 219-225

    • DOI

      10.1111/1348-0421.12764

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Tree of motility : A proposed history of motility systems in the tree of life2020

    • Author(s)
      M Miyata, R C Robinson, T QP Uyeda, Y Fukumori, S Fukushima, S Haruta, M Homma, K Inaba, M Ito, C Kaito, K Kato, TKenri, Y Kinosita, S Kojima, T Minamino, H Mori, S Nakamura, D Nakane, K Nakayama, M Nishiyama, S Shibata, K Shimabukuro, M Tamakoshi, A Taoka, Y Tashiro, ITulum, HiWada, K Wakabayashi
    • Journal Title

      Genes to Cells

      Volume: 25 Issue: 1 Pages: 6-21

    • DOI

      10.1111/gtc.12737

    • NAID

      130007881664

    • URL

      https://ocu-omu.repo.nii.ac.jp/records/2019864

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dataset for de novo transcriptome assembly of the African bullfrog Pyxicephalus adspersus2020

    • Author(s)
      Yoshida N, Kaito C.
    • Journal Title

      Data Brief.

      Volume: 30 Pages: 105388-105388

    • DOI

      10.1016/j.dib.2020.105388

    • NAID

      120006866144

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Staphylococcus aureus aggregation in the plasma fraction of silkworm hemolymph2019

    • Author(s)
      Ryuno, H., Nigo, F., Naguro, I., Sekimizu, K., and Kaito, C.
    • Journal Title

      PLoS One

      Volume: 14 Issue: 5 Pages: e0217517-e0217517

    • DOI

      10.1371/journal.pone.0217517

    • NAID

      120006780367

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of 2H phosphoesterase superfamily proteins with 2′-CPDase activity2019

    • Author(s)
      Mitsutomi Shuhei、Akimitsu Nobuyoshi、Sekimizu Kazuhisa、Kaito Chikara
    • Journal Title

      Biochimie

      Volume: 165 Pages: 235

    • DOI

      10.1016/j.biochi.2019.08.008

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] 病原体進化の要因となる病原体宿主相互作用2021

    • Author(s)
      垣内力
    • Organizer
      第94回日本生化学会大会 シンポジウム(オンライン開催)
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] MRSAの病原性制御機構2020

    • Author(s)
      垣内力
    • Organizer
      第94回日本感染症学会総会 シンポジウム
    • Related Report
      2020 Annual Research Report
    • Invited

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Published: 2019-04-18   Modified: 2023-01-30  

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