Comprehensive analysis of cancer-specific antigens recognized by tumor-infiltrating T cells

• Project/Area Number: 19H03522
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 50020:Tumor diagnostics and therapeutics-related
• Research Institution: Japanese Foundation for Cancer Research
• Principal Investigator: KIYOTANI Kazuma 公益財団法人がん研究会, がんプレシジョン医療研究センター 免疫ゲノム医療開発プロジェクト, 主任研究員 (30433642)
• Co-Investigator(Kenkyū-buntansha): 黒田 大祐 東京大学, 大学院工学系研究科(工学部), 講師 (60756732)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2021: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2020: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2019: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 腫瘍浸潤T細胞 / ネオアンチゲン / T細胞受容体 / ネオ抗原

Outline of Research at the Start

がん局所に浸潤したT細胞(腫瘍浸潤T細胞) はsomatic変異由来のneoantigenなど様々ながん特異的抗原を認識し、抗腫瘍効果を示す。本研究では、腫瘍浸潤T細胞がneoantigenを含めどのような抗原を認識しているかを網羅的に解析し、その抗原とT細胞受容体を予測するシステムを開発することを目的としている。新鮮手術検体を用いて、1) 腫瘍浸潤T細胞レパトア解析によるがん特異的T細胞の同定、2) タンパク質の立体構造に基づく分子シミュレーションと機械学習によるがん特異的T細胞の予測、の二つの項目について解析する。

Outline of Final Research Achievements

The purpose of this study was to screen antigens of tumor-infiltrating T cells, and establish prediction system for antigen-TCR interactions. We collected fresh surgical tissues of colorectal cancer and isolated tumor-infiltrating T cells and patient-derived cancer cells (PDCs). We established the system to enrich tumor-reactive T cells by co-culture of tumor-infiltrating T cells and PDCs, and obtained TCRs from tumor-reactive T cells. Using TCR-engineered T cells, we confirmed that TCRs from tumor-reactive T cells are actually tumor-reactive, but not always neoantigen-reactive.

Academic Significance and Societal Importance of the Research Achievements

本研究では、腫瘍浸潤T細胞と患者由来がん細胞の共培養により、腫瘍反応性T細胞を増殖できる系を開発した。この方法は、腫瘍内の腫瘍反応性T細胞の同定、さらには腫瘍反応性T細胞を用いた治療法開発にもつながると考えられる。さらに、T細胞が認識する抗原の網羅的な探索系の開発も進めており、この系は様々な用途に利用可能であると考えている。

Research Products

• [Journal Article] Perioperative circulating tumor DNA enables the identification of patients with poor prognosis in upper tract urothelial carcinoma (2022)
  • Author(s): Nakano K, Koh Y, Yamamichi G, Yumiba S, Tomiyama E, Matsushita M, Hayashi Y, Wang C, Ishizuya Y, Yamamoto Y, Kato T, Hatano K, Kawashima A, Ujike T, Fujita K, Kiyotani K, Katayama K, Yamaguchi R, Imoto S, Imamura R, Nonomura N, Uemura M
  • Journal Title: Cancer Science Volume: - Issue: 5 Pages: 1830-1842
  • DOI: 10.1111/cas.15334
  • Peer Reviewed
• [Journal Article] Current advances in biopharmaceutical informatics: guidelines, impact and challenges in the computational developability assessment of antibody therapeutics (2022)
  • Author(s): Khetan R, Curtis R, Deane CM, Hadsund JT, Kar U, Krawczyk K, Kuroda D, Robinson SA, Sormanni P, Tsumoto K, Warwicker J, Martin ACR.
  • Journal Title: mAbs Volume: 14 Issue: 1 Pages: 2020082-2020082
  • DOI: 10.1080/19420862.2021.2020082
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Oligo(N‐methylalanine) as a Peptide‐Based Molecular Scaffold with a Minimal Structure and High Density of Functionalizable Sites (2022)
  • Author(s): Yokomine Marin、Morimoto Jumpei、Fukuda Yasuhiro、Shiratori Yota、Kuroda Daisuke、Ueda Takumi、Takeuchi Koh、Tsumoto Kouhei、Sando Shinsuke
  • Journal Title: Angewandte Chemie International Edition Volume: 61 Issue: 14 Pages: 6994-7000
  • DOI: 10.1002/anie.202200119
  • Peer Reviewed
• [Journal Article] Precision Medicine for Colorectal Cancer with Liquid Biopsy and Immunotherapy. (2021)
  • Author(s): Nagayama S, Low SK, Kiyotani K, Nakamura Y.
  • Journal Title: Cancers Volume: 12 Issue: 19 Pages: 4803-4803
  • DOI: 10.3390/cancers13194803
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Personalized immunotherapy in cancer precision medicine (2021)
  • Author(s): Kiyotani K, Toyoshima Y, Nakamura Y
  • Journal Title: Cancer Biology and Medicine Volume: 18 Issue: - Pages: 955-965
  • DOI: 10.20892/j.issn.2095-3941.2021.0032
  • Peer Reviewed / Open Access
• [Journal Article] Efficacy of intranodal neoantigen peptide-pulsed dendritic cell vaccine monotherapy in patients for advanced solid tumors; retrospective analysis. (2021)
  • Author(s): Morisaki T, Morisaki T, Kubo M, Onishi H, Hirano T, Morisaki S, Eto M, Monji K, Takeuchi A, Nakagawa S, Tanaka H, Koya N, Umebayashi M, Tsujimura K, Yew PY, Yoshimura S, Kiyotani K, Nakamura Y
  • Journal Title: Anticancer Res Volume: 41(8) Issue: 8 Pages: 4101-4115
  • DOI: 10.21873/anticanres.15213
  • Peer Reviewed
• [Journal Article] Immunogenomics in personalized cancer treatments (2021)
  • Author(s): Kiyotani K, Toyoshima Y, Nakamura Y.
  • Journal Title: Journal of Human Genetics Volume: 66 Issue: 9 Pages: 901-907
  • DOI: 10.1038/s10038-021-00950-w
  • Peer Reviewed
• [Journal Article] Neoantigens elicit T cell responses in breast cancer (2021)
  • Author(s): Morisaki T, Kubo M, Umebayashi M, Yew PY, Yoshimura S, Park JH, Kiyotani K, Kai M, Yamada M, Oda Y, Nakamura Y, Morisaki T, Nakamura M.
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 13590-13590
  • DOI: 10.1038/s41598-021-91358-1
  • Peer Reviewed
• [Journal Article] Generation of neoantigen-specific T cells for adoptive cell transfer for treating head and neck squamous cell carcinoma (2021)
  • Author(s): Wei T, Leisegang M, Xia M, Kiyotani K, Li N, Zeng C, Deng C, Jiang J, Harada M, Agrawal N, Li L, Qi H, Nakamura Y, Ren L.
  • Journal Title: OncoImmunology Volume: 10 Issue: 1 Pages: 1929726-1929726
  • DOI: 10.1080/2162402x.2021.1929726
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genomic alterations in gynecological malignancies: histotype-associated driver mutations, molecular subtyping schemes, and tumorigenic mechanisms (2021)
  • Author(s): Mori S, Gotoh O, Kiyotani K, Low SK
  • Journal Title: Journal of Human Genetics Volume: 66 Issue: 9 Pages: 853-868
  • DOI: 10.1038/s10038-021-00940-y
  • Peer Reviewed
• [Journal Article] Genomic and Pathological Characterization of Multiple Renal Cell Carcinoma Regions in Patient With Tuberous Sclerosis Complex: A Case Report (2021)
  • Author(s): Yamamoto Tetsuya、Kato Taigo、Hatano Koji、Kawashima Atsunari、Ujike Takeshi、Fukuhara Shinichiro、Kiuchi Hiroshi、Imamura Ryoichi、Ibuki Naokazu、Kiyotani Kazuma、Kurashige Masako、Morii Eichi、Fujita Kazutoshi、Nonomura Norio、Uemura Motohide
  • Journal Title: Frontiers in Oncology Volume: 11 Pages: 691996-691996
  • DOI: 10.3389/fonc.2021.691996
  • Peer Reviewed
• [Journal Article] Monitoring epidermal growth factor receptor C797S mutation in Japanese non?small cell lung cancer patients with serial cell‐free DNA evaluation using digital droplet PCR (2021)
  • Author(s): Ariyasu Ryo、Uchibori Ken、Sasaki Takaaki、Tsukahara Mika、Kiyotani Kazuma、Yoshida Ryohei、Ono Yusuke、Kitazono Satoru、Ninomiya Hironori、Ishikawa Yuichi、Mizukami Yusuke、Yanagitani Noriko、Fujita Naoya、Nishio Makoto、Katayama Ryohei
  • Journal Title: Cancer Science Volume: - Issue: 6 Pages: 2371-2380
  • DOI: 10.1111/cas.14879
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Peptoid-based reprogrammable template for cell-permeable inhibitors of protein?protein interactions (2021)
  • Author(s): Fukuda Y, Yokomine M, Kuroda D, Tsumoto K, Morimoto J, Sando S.
  • Journal Title: Chemical Science Volume: 12 Issue: 40 Pages: 13292-13300
  • DOI: 10.1039/d1sc01560e
  • Peer Reviewed / Open Access
• [Journal Article] Delicate balance among thermal stability, binding affinity, and conformational space explored by single-domain VHH antibodies (2021)
  • Author(s): Ikeuchi E, Kuroda D, Nakakido M, Murakami A, Tsumoto K.
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 20624-20624
  • DOI: 10.1038/s41598-021-98977-8
  • Peer Reviewed / Open Access
• [Journal Article] Microsecond molecular dynamics suggest that a non-synonymous mutation, frequently observed in patients with mild symptoms in Tokyo, alters dynamics of the SARS-CoV-2 main protease (2021)
  • Author(s): Kuroda D, Tsumoto K.
  • Journal Title: Biophysics and Physicobiology Volume: 18 Issue: 0 Pages: 215-222
  • DOI: 10.2142/biophysico.bppb-v18.022
  • NAID: 130008088919
  • ISSN: 2189-4779
  • Peer Reviewed / Open Access
• [Journal Article] Regulation of cadherin dimerization by chemical fragments as a trigger to inhibit cell adhesion (2021)
  • Author(s): Senoo Akinobu、Ito Sho、Nagatoishi Satoru、Saito Yutaro、Ueno Go、Kuroda Daisuke、Yoshida Kouhei、Tashima Takumi、Kudo Shota、Sando Shinsuke、Tsumoto Kouhei
  • Journal Title: Communications Biology Volume: 4 Issue: 1 Pages: 1041-1041
  • DOI: 10.1038/s42003-021-02575-3
  • Peer Reviewed / Open Access
• [Journal Article] Mechanism of dimerization and structural features of human LI-cadherin (2021)
  • Author(s): Yui Anna、Caaveiro Jose M.M.、Kuroda Daisuke、Nakakido Makoto、Nagatoishi Satoru、Goda Shuichiro、Maruno Takahiro、Uchiyama Susumu、Tsumoto Kouhei
  • Journal Title: Journal of Biological Chemistry Volume: 297 Issue: 3 Pages: 101054-101054
  • DOI: 10.1016/j.jbc.2021.101054
  • Peer Reviewed / Open Access
• [Journal Article] Anion solvation enhanced by positive supercharging mutations preserves thermal stability of an antibody in a wide pH range (2021)
  • Author(s): Kasahara Keisuke、Kuroda Daisuke、Tanabe Aki、Kawade Raiji、Nagatoishi Satoru、Tsumoto Kouhei
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 563 Pages: 54-59
  • DOI: 10.1016/j.bbrc.2021.05.053
  • Peer Reviewed
• [Journal Article] Peripheral T cell receptor repertoire features predict durable responses to anti-PD-1 inhibitor monotherapy in advanced renal cell carcinoma (2021)
  • Author(s): Kato T, Kiyotani K, Tomiyama E, Koh Y, Matsushita M, Hayashi Y, Nakano K, Ishizuya Y, Wang C, Hatano K, Kawashima A, Ujike T, Fujita K, Nonomura N, Uemura M.
  • Journal Title: OncoImmunology Volume: 10 Issue: 1 Pages: 1862948-1862948
  • DOI: 10.1080/2162402x.2020.1862948
  • Peer Reviewed / Open Access
• [Journal Article] Immunogenomic landscape of gynecologic carcinosarcoma (2021)
  • Author(s): Gotoh Osamu、Kiyotani Kazuma、Chiba Tomohiro、Sugiyama Yuko、Takazawa Yutaka、Nemoto Kensaku、Kato Kazuyoshi、Tanaka Norio、Nomura Hidetaka、Hasegawa Kosei、Fujiwara Keiichi、Takamatsu Shiro、Matsumura Noriomi、Noda Tetsuo、Mori Seiichi
  • Journal Title: Gynecologic Oncology Volume: 160 Issue: 2 Pages: 547-556
  • DOI: 10.1016/j.ygyno.2020.11.030
  • Peer Reviewed / Open Access
• [Journal Article] Thermodynamic Dissection of Potency and Selectivity of Cytosolic Hsp90 Inhibitors (2021)
  • Author(s): Yoshimura Chihoko、Nagatoishi Satoru、Kuroda Daisuke、Kodama Yasuo、Uno Takao、Kitade Makoto、Chong-Takata Khoontee、Oshiumi Hiromi、Muraoka Hiromi、Yamashita Satoshi、Kawai Yuichi、Ohkubo Shuichi、Tsumoto Kouhei
  • Journal Title: Journal of Medicinal Chemistry Volume: 64 Issue: 5 Pages: 2669-2677
  • DOI: 10.1021/acs.jmedchem.0c01715
  • Peer Reviewed
• [Journal Article] Structural basis for antigen recognition by methylated lysine?specific antibodies (2021)
  • Author(s): Ishii M, Nakakido M, Caaveiro JMM, Kuroda D, Okumura CJ, Maruyama T, Entzminger K, Tsumoto K.
  • Journal Title: Journal of Biological Chemistry Volume: 296 Pages: 100176-100176
  • DOI: 10.1074/jbc.ra120.015996
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Potential Mechanism of Anticancer Immune Response Coincident With Immune-related Adverse Events in Patients With Renal Cell Carcinoma (2020)
  • Author(s): Kato T, Tomiyama E, Koh Y, Matsushita M, Hayashi Y, Nakano K, Ishizuya YU, Wang C, Hatano K, Kawashima A, Ujike T, Kawasaki K, Morii E, Gotoh K, Eguchi H, Kiyotani K, Fujita K, Nonomura N, Uemura M.
  • Journal Title: Anticancer Research Volume: 40 Issue: 9 Pages: 4875-4883
  • DOI: 10.21873/anticanres.14490
  • Peer Reviewed / Open Access
• [Journal Article] T-cell complexity and density are associated with sensitivity to neoadjuvant chemoradiotherapy in patients with rectal cancer (2020)
  • Author(s): Akiyoshi T, Gotoh O, Tanaka N, Kiyotani K, Yamamoto N, Ueno M, Fukunaga Y, Mori S.
  • Journal Title: Cancer Immunology, Immunotherapy Volume: 70 Issue: 2 Pages: 509-518
  • DOI: 10.1007/s00262-020-02705-6
  • Peer Reviewed / Open Access
• [Journal Article] SARS-CoV-2 genomic variations associated with mortality rate of COVID-19 (2020)
  • Author(s): Toyoshima Y, Nemoto K, Matsumoto S, Nakamura Y, Kiyotani K.
  • Journal Title: Journal of Human Genetics Volume: 65 Issue: 12 Pages: 1075-1082
  • DOI: 10.1038/s10038-020-0808-9
  • Peer Reviewed / Open Access
• [Journal Article] Intranodal Administration of Neoantigen Peptide-loaded Dendritic Cell Vaccine Elicits Epitope-specific T Cell Responses and Clinical Effects in a Patient with Chemorefractory Ovarian Cancer with Malignant Ascites (2020)
  • Author(s): Morisaki Takashi、Hikichi Tetsuro、Onishi Hideya、Morisaki Takafumi、Kubo Makoto、Hirano Tatsuya、Yoshimura Sachiko、Kiyotani Kazuma、Nakamura Yusuke
  • Journal Title: Immunological Investigations Volume: 13 Issue: 5 Pages: 1-18
  • DOI: 10.1080/08820139.2020.1778721
  • Peer Reviewed / Open Access
• [Journal Article] Clinical significance of clonal hematopoiesis in the interpretation of blood liquid biopsy (2020)
  • Author(s): Chan Hiu Ting、Nagayama Satoshi、Chin Yoon Ming、Otaki Masumi、Hayashi Rie、Kiyotani Kazuma、Fukunaga Yosuke、Ueno Masashi、Nakamura Yusuke、Low Siew‐Kee
  • Journal Title: Molecular Oncology Volume: 14 Issue: 8 Pages: 1719-1730
  • DOI: 10.1002/1878-0261.12727
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Bioinformatic prediction of potential T cell epitopes for SARS-Cov-2 (2020)
  • Author(s): Kiyotani K, Toyoshima Y, Nemoto K, Nakamura Y.
  • Journal Title: Journal of Human Genetics Volume: 65 Issue: 7 Pages: 569-575
  • DOI: 10.1038/s10038-020-0771-5
  • Peer Reviewed / Open Access
• [Journal Article] Cooperation of genes in HPV16 E6/E7-dependent cervicovaginal carcinogenesis trackable by endoscopy and independent of exogenous estrogens or carcinogens (2020)
  • Author(s): Paula Bottinger, Kiyotani Kazuma, et al.
  • Journal Title: Carcinogenesis Volume: . Issue: 11 Pages: 1605-1615
  • DOI: 10.1093/carcin/bgaa027
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Engineering Stability, Viscosity, and Immunogenicity of Antibodies by Computational Design (2020)
  • Author(s): Kuroda Daisuke、Tsumoto Kouhei
  • Journal Title: Journal of Pharmaceutical Sciences Volume: 109 Issue: 5 Pages: 1631-1651
  • DOI: 10.1016/j.xphs.2020.01.011
  • Peer Reviewed / Open Access
• [Journal Article] Per-Residue Program of Multiple Backbone Dihedral Angles of β-Peptoids via Backbone Substitutions (2020)
  • Author(s): Jumpei Morimoto, Jungyeon Kim, Daisuke Kuroda, Satoru Nagatoishi, Kouhei Tsumoto, Shinsuke Sando
  • Journal Title: Journal of the American Chemical Society Volume: 142 Issue: 5 Pages: 2277-2284
  • DOI: 10.1021/jacs.9b10496
  • Peer Reviewed
• [Journal Article] Impact of TCR Diversity on the Development of Transplanted or Chemically Induced Tumors (2019)
  • Author(s): Schreiber K, Karrison G, Wolf SP, Kiyotani K, Steiner M, Littmann ER, Pamer EG, Kammertoens T, Schreiber H, Leisegang M.
  • Journal Title: Cancer Immunology Research Volume: 8 Issue: 2 Pages: 192-202
  • DOI: 10.1158/2326-6066.cir-19-0567
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Tamoxifen Metabolism and Breast Cancer Recurrence: A Question Unanswered by CYPTAM (2019)
  • Author(s): Goetz MP, Suman VJ, Nakamura Y, Kiyotani K, Jordan VC, Ingle JN.
  • Journal Title: Journal of Clinical Oncology Volume: 37 Issue: 22 Pages: 1982-1983
  • DOI: 10.1200/jco.19.00504
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Diagnostic utility of STAT6YE361 expression in classical Hodgkin lymphoma and related entities (2019)
  • Author(s): Slambrouck CV, Huh J, Suh C, Song JY, Menon MP, Sohani AR, Duffield AS, Goldberg RC, Dama P, Kiyotani K, Godfrey J, Fitzpatrick C, Kline J, Smith SM, Jaffe ES, Hartmann S, Venkataraman G.
  • Journal Title: Modern Pathology Volume: 33 Issue: 5 Pages: 834-845
  • DOI: 10.1038/s41379-019-0428-0
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Secreted PD-L1 variants mediate resistance to PD-L1 blockade therapy in non-small cell lung cancer (2019)
  • Author(s): Gong Bo, Kiyotani Kazuma, Sakata Seiji, Nagano Seiji, Kumehara Shun, Baba Satoko, Besse Benjamin, Yanagitani Noriko, Friboulet Luc, Nishio Makoto, Takeuchi Kengo, Kawamoto Hiroshi, Fujita Naoya, Katayama Ryohei
  • Journal Title: The Journal of Experimental Medicine Volume: 216 Issue: 4 Pages: 982-1000
  • DOI: 10.1084/jem.20180870
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effects of a remote mutation from the contact paratope on the structure of CDR-H3 in the anti-HIV neutralizing antibody PG16 (2019)
  • Author(s): Kondo Hiroko X.、Kiribayashi Ryo、Kuroda Daisuke、Kohda Jiro、Kugimiya Akimitsu、Nakano Yasuhisa、Tsumoto Kouhei、Takano Yu
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 19840-19840
  • DOI: 10.1038/s41598-019-56154-y
  • Peer Reviewed / Open Access
• [Journal Article] How the protonation state of a phosphorylated amino acid governs molecular recognition: insights from classical molecular dynamics simulations (2019)
  • Author(s): Kawade Raiji、Kuroda Daisuke、Tsumoto Kouhei
  • Journal Title: FEBS Letters Volume: 594 Issue: 5 Pages: 903-912
  • DOI: 10.1002/1873-3468.13674
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A Peptoid with Ectended Shape in Water (2019)
  • Author(s): Morimoto J, Fukuda Y, Kuroda D, Watanabe T, Yoshida F, Asada M, Nakamura T, Senoo A, Nagatoishi S, Tsumoto K, *Sando S
  • Journal Title: J Am Chem Soc. Volume: 141 Issue: 37 Pages: 14612-14623
  • DOI: 10.1021/jacs.9b04371
  • Peer Reviewed
• [Journal Article] An epitope‐directed antibody affinity maturation system utilizing mammalian cell survival as readout (2019)
  • Author(s): Eguchi Akihiro、Nakakido Makoto、Nagatoishi Satoru、Kuroda Daisuke、Tsumoto Kouhei、Nagamune Teruyuki、Kawahara Masahiro
  • Journal Title: Biotechnology and Bioengineering Volume: 116 Issue: 7 Pages: 1742-1751
  • DOI: 10.1002/bit.26965
  • Peer Reviewed
• [Presentation] Screening of targetable neoantigens shared by multiple cancer patients. (2021)
  • Author(s): Kiyotani K, Nakamura Y.
  • Organizer: 第80回日本癌学会
• [Presentation] Early dynamics of T cell clonotypes as potential surrogate biomarker for immune checkpoint inhibitors. (2021)
  • Author(s): Kato T, Kiyotani K, Yamamoto A, Uemura T, Yamamichi G, Tomiyama E, Koh Y, Matsushita M, Hatano K, Kawashima A, Uemura M, Nonomura N.
  • Organizer: 第80回日本癌学会
• [Presentation] Generation of neoantigen-specific T cells for adoptive cell transfer for treating head and neck squamous cell carcinoma. (2021)
  • Author(s): Lili R, Wei T, Leisegang M, Xia M, Kiyotani K, Li N, Zeng C, Deng C, Jiang J, Harada M, Agrawal N, Li L, Qi H, Nakamura Y.
  • Organizer: 第80回日本癌学会
• [Presentation] Epigenetic alteration of SALL3 contributes to chemoresistance in triple-negative breast cancer. (2021)
  • Author(s): Matsushita Y, Komatsu M, Kiyotani K, Niinuma T, Suzuki H, Yoshimaru T, Tajima A, Imoto I, Honda J, Furukawa Y, Nakamura Y, Miyoshi Y, Sasa M, Katagiri T.
  • Organizer: 第80回日本癌学会
• [Presentation] T細胞受容体レパトア解析を用いた免疫チェックポイント阻害剤奏効予測マーカーの開発. (2021)
  • Author(s): 加藤大悟, 波多野浩士, 河嶋厚成, 植村元秀, 清谷一馬, 中村祐輔, 野々村祝夫.
  • Organizer: 第34回日本バイオセラピィ学会学術集会総会.
• [Presentation] Cancer-reactive/neoantigen-specific T cells in peripheral blood and tumor tissues of cancer patients (2020)
  • Author(s): Kazuma Kiyotani, Yusuke Nakamura
  • Organizer: 第79回日本癌学会
• [Presentation] Combined targeting of tumor-stroma and cancer cells by T cells is essential for eradication of solid tumors (2020)
  • Author(s): Steven P. Wolf, Vasiliki Anastasopoulou, Madeline Steiner, Markus I. Diehl, Kimberley Borutta, Boris Engels, Poh Yin Yew, Kazuma Kiyotani, Yusuke Nakamura, Karin Schreiber, Hans Schreiber, Matthias Leisegang
  • Organizer: 第79回日本癌学会
• [Presentation] リンパ節中の腫瘍反応性T細胞の同定と機能解析 (2020)
  • Author(s): 清谷一馬、岡村和美、建智博、長山聡、中村祐輔
  • Organizer: 第24回日本がん免疫学会総会
• [Presentation] Screening of cancer-reactive T cells in lymph nodes in colorectal cancer patients (2020)
  • Author(s): Kazuma Kiyotani, Kazumi Okamura, Satoshi Nagayama, Yusuke Nakamura
  • Organizer: AACR Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] ゲノムシーケンスデータからのネオアンチゲン予測と特異的T細胞の誘導 (2020)
  • Author(s): 清谷一馬、中村祐輔
  • Organizer: 第24回バイオ治療法研究会学術講演会
  • Invited
• [Presentation] Comparison of TCR repertoires between cancer tissues and lymph nodes in colorectal cancer patients. (2019)
  • Author(s): Kiyotani K, Matsuda T, Miyauchi E, Hsu Y, Nagayama S, Zewde M, Park JH, Kato T, Harada M, Suzuki N, Nagano H, Hazama S, Nakamura Y.
  • Organizer: AACR Annual Meeting 2019
  • Int'l Joint Research
• [Presentation] Identification of cancer-reactive/neoantigen-specific T cells in peripheral blood and tumor tissues of cancer patients. (2019)
  • Author(s): Kiyotani K
  • Organizer: 6th Annual Meeting of Colegio Mexicano para la Investigacion del Cancer
  • Int'l Joint Research / Invited
• [Presentation] Progress and future of cancer immunotherapy (2019)
  • Author(s): Kiyotani K
  • Organizer: 第78回日本癌学会
• [Presentation] Screening of cancer-reactive/neoantigen-specific T cells in peripheral blood and tumor tissues of cancer patients. (2019)
  • Author(s): Kiyotani K, Nakamura Y.
  • Organizer: 第78回日本癌学会
• [Presentation] Secreted PD-L1 variants caused by aberrant splicing mediate antiPD-L1 antibody resistance. (2019)
  • Author(s): Gong B, Kiyotani K, Sakata S, Nishio M, Takeuchi K, Kawamoto H, Fujita N, Katayama R.
  • Organizer: 第78回日本癌学会
• [Presentation] TCR repertoires in lung adenocarcinomas with and without EGFR mutation. (2019)
  • Author(s): Miyauchi E, Matsuda T, Kiyotani K, Low SK, Tsukita Y, Ichinose M, Sakurada A, Okada Y, Saito R, Nakamura Y.
  • Organizer: 第78回日本癌学会
• [Presentation] Clinical significance of clonal hematopoiesis in the interpretation of blood liquid biopsy. (2019)
  • Author(s): Chan HT, Nagayama S, Chin YM, Hayashi R, Kiyotani K, Nakamura Y, Low SK
  • Organizer: 第78回日本癌学会