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Elucidation of the pathogenic mechanism and screening of therapeutic compounds in prion disease

Research Project

Project/Area Number 19H03548
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 51030:Pathophysiologic neuroscience-related
Research InstitutionThe University of Tokushima

Principal Investigator

SAKAGUCHI Suehiro  徳島大学, 先端酵素学研究所, 教授 (60274635)

Co-Investigator(Kenkyū-buntansha) 千田 淳司  徳島大学, 先端酵素学研究所, 助教 (20437651)
原 英之  徳島大学, 先端酵素学研究所, 助教 (40469953)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2021: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2020: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2019: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Keywordsプリオン / プリオン病 / アトラクチン / ソーチリン / エタノールアミン / トランスジェニックマウス / ポストゴルジ輸送 / ポストゴルジ小胞輸送 / 神経変性
Outline of Research at the Start

プリオン病の治療法を開発することは緊急な研究課題である。このためには、プリオン病の病態メカニズムを解明することが重要である。申請者らは、1)プリオン病では細胞膜蛋白質のポストゴルジ輸送が障害されこと、2)プリオン病では蛋白質輸送関連分子ソーチリンが減少しソーチリンを介した蛋白質の輸送が障害されこと、3)ソーチリンを過剰発現しソーチリンによる輸送を改善するとプリオンの産生が抑制されることを報告した。そこで本研究では、プリオンによるポストゴルジ輸送障害およびソーチリンの減少による輸送障害のメカニズムを明らかにし、ソーチリンの減少を改善しプリオンの産生を抑制する化合物を同定する。

Outline of Final Research Achievements

In this study, we produced transgenic (Tg) mice expressing either attractin or sortilin and intracerebrally inoculated prions into them. Attractin-Tg mice developed disease earlier than control mice and sortilin-Tg mice succumbed to disease later than control mice. These results suggest that attractin could promote the pathogenesis of prion disease while sortilin could retard disease progression. We also found that golgin-97, which is expressed in the trans-Golgi network (TGN), could interact with sortilin-containing vesicles. However, golgin-97 was reduced in prion-infected cells. These results suggest that prions could reduce golgin-97, thereby disturbing the retrograde transport of sortilin to the TGN in infected cells. We also identified ethanolamine as a new anti-prion agent.

Academic Significance and Societal Importance of the Research Achievements

プリオン病の病態メカニズムを明らかにし、プリオン病の治療法を開発することは緊急の課題である。本研究では、アトラクチンがプリオン病の進行を促進し、ソーチリンは逆にプリオン病の進行を抑制することを明らかにした。この結果は、アトラクチンとソーチリンがプリオン病の病態に関与することを示し、アトラクチンの機能抑制やソーチリンの活性化がプリオン病の治療法開発につながる可能性を示した。また本研究では、新規のプリオン病の治療薬としてエタノールアミンを同定した。以上の研究成果は、プリオン病の病態メカニズムの解明やプリオン病の治療法開発に貢献するものであり、学術的意義及び社会的意義がある。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (18 results)

All 2022 2021 2020 2019 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 10 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (2 results)

  • [Journal Article] The first non-prion pathogen identified: neurotropic influenza virus2022

    • Author(s)
      Sakaguchi Suehiro、Hara Hideyuki
    • Journal Title

      Prion

      Volume: 16 Issue: 1 Pages: 1-6

    • DOI

      10.1080/19336896.2021.2015224

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Virus Infection, Genetic Mutations, and Prion Infection in Prion Protein Conversion2021

    • Author(s)
      Hara Hideyuki、Sakaguchi Suehiro
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 22 Pages: 12439-12439

    • DOI

      10.3390/ijms222212439

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ethanolamine Is a New Anti-Prion Compound2021

    • Author(s)
      Uchiyama Keiji、Hara Hideyuki、Chida Junji、Pasiana Agriani Dini、Imamura Morikazu、Mori Tsuyoshi、Takatsuki Hanae、Atarashi Ryuichiro、Sakaguchi Suehiro
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 21 Pages: 11742-11742

    • DOI

      10.3390/ijms222111742

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Neurotropic influenza A virus infection causes prion protein misfolding into infectious prions in neuroblastoma cells2021

    • Author(s)
      Hara Hideyuki、Chida Junji、Uchiyama Keiji、Pasiana Agriani Dini、Takahashi Etsuhisa、Kido Hiroshi、Sakaguchi Suehiro
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 10109-10109

    • DOI

      10.1038/s41598-021-89586-6

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Vaporized Hydrogen Peroxide and Ozone Gas Synergistically Reduce Prion Infectivity on Stainless Steel Wire2021

    • Author(s)
      Hara Hideyuki、Chida Junji、Pasiana Agriani Dini、Uchiyama Keiji、Kikuchi Yutaka、Naito Tomoko、Takahashi Yuichi、Yamamura Junji、Kuromatsu Hisashi、Sakaguchi Suehiro
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 6 Pages: 3268-3268

    • DOI

      10.3390/ijms22063268

    • NAID

      120007164927

    • Related Report
      2021 Annual Research Report 2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Strain-Dependent Prion Infection in Mice Expressing Prion Protein with Deletion of Central Residues 91?1062020

    • Author(s)
      Uchiyama Keiji、Miyata Hironori、Yamaguchi Yoshitaka、Imamura Morikazu、Okazaki Mariya、Pasiana Agriani Dini、Chida Junji、Hara Hideyuki、Atarashi Ryuichiro、Watanabe Hitomi、Kondoh Gen、Sakaguchi Suehiro
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 19 Pages: 7260-7260

    • DOI

      10.3390/ijms21197260

    • NAID

      120006959718

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] N-Terminal Regions of Prion Protein: Functions and Roles in Prion Diseases2020

    • Author(s)
      Hara Hideyuki、Sakaguchi Suehiro
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 17 Pages: 6233-6233

    • DOI

      10.3390/ijms21176233

    • NAID

      120006959719

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Impairment of cerebellar long-term depression and GABAergic transmission in prion protein deficient mice ectopically expressing PrPLP/Dpl2020

    • Author(s)
      Yasushi Kishimoto、Moritoshi Hirono、Ryuichiro Atarashi、Suehiro Sakaguchi、Tohru Yoshioka、Shigeru Katamine、Yutaka Kirino
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 15900-15900

    • DOI

      10.1038/s41598-020-72753-6

    • NAID

      120006959725

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice2020

    • Author(s)
      Chida Junji、Hara Hideyuki、Uchiyama Keiji、Takahashi Etsuhisa、Miyata Hironori、Kosako Hidetaka、Tomioka Yukiko、Ito Toshihiro、Horiuchi Hiroyuki、Matsuda Haruo、Kido Hiroshi、Sakaguchi Suehiro
    • Journal Title

      PLOS Pathogens

      Volume: 16 Issue: 8 Pages: e1008823-e1008823

    • DOI

      10.1371/journal.ppat.1008823

    • NAID

      120006956844

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prion Protein is a Novel Modulator of Influenza: Potential Implications for Anti-Influenza Therapeutics2020

    • Author(s)
      Sakaguchi Suehiro、Chida Junji
    • Journal Title

      Current Issues in Molecular Biology

      Volume: 37 Pages: 21-32

    • DOI

      10.21775/cimb.037.021

    • NAID

      120006979198

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The N-Terminal Polybasic Region of Prion Protein Is Crucial in Prion Pathogenesis Independently of the Octapeptide Repeat Region.2020

    • Author(s)
      Nandita Rani Das, Hironori Miyata, Hideyuki Hara, Junji Chida, Keiji Uchiyama, Kentaro Masujin, Hitomi Watanabe, Gen Kondoh, Suehiro Sakaguchi
    • Journal Title

      Molecular Neurobiology

      Volume: 57 Pages: 1203-1216

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Presentation] Prion protein in health and disease2022

    • Author(s)
      Suehiro Sakaguchi
    • Organizer
      The 12th BRI International Symposium(第12回新潟大学脳研究所共同拠点国際シンポジウム)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] インフルエンザウイルス感染は神経細胞において感染性プリオンの産生を引き起こす2021

    • Author(s)
      原 英之、千田 淳司、坂口 末廣
    • Organizer
      第 44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Prion protein in influenza virus infection.2021

    • Author(s)
      Suehiro Sakaguchi
    • Organizer
      APPS2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] プリオン蛋白質はインフルエンザAウイルス感染による重症化を軽減する2019

    • Author(s)
      千田 淳司、原 英之、坂口 末廣
    • Organizer
      第 42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ウイルス感染を用いたプリオン病発症機構の解明2019

    • Author(s)
      原 英之、千田 淳司、坂口 末廣
    • Organizer
      第 42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Remarks] 神経変性病態学分野|徳島大学先端酵素学研究所ホームページ

    • URL

      https://www.iams.tokushima-u.ac.jp/lab/sakaguchi/

    • Related Report
      2021 Annual Research Report
  • [Remarks] 徳島大学先端酵素学研究所神経変性病態学分野

    • URL

      http://www.iams.tokushima-u.ac.jp/lab/sakaguchi/

    • Related Report
      2019 Annual Research Report

URL: 

Published: 2019-04-18   Modified: 2023-01-30  

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