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An ALS2-linked functional cellular network-based study on molecular mechanisms of selective upper motor neuron degeneration in ALS

Research Project

Project/Area Number 19H03551
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 51030:Pathophysiologic neuroscience-related
Research InstitutionTokai University

Principal Investigator

Hadano Shinji  東海大学, 医学部, 教授 (60281375)

Co-Investigator(Kenkyū-buntansha) 木村 啓志  東海大学, マイクロ・ナノ研究開発センター, 教授 (40533625)
大友 麻子  東海大学, 医学部, 講師 (50535226)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2022: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2021: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2020: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2019: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords筋萎縮性側索硬化症 / 運動ニューロン / ALS2 / 細胞内オルガネラ動態 / iPS細胞 / マイクロ流体デバイス
Outline of Research at the Start

筋萎縮性側索硬化症(ALS)は、上位および下位運動ニューロンの選択的変性・脱落を特徴とする神経変性疾患である。近年、下位運動ニューロンの変性メカニズム研究が格段に進展しているなか、上位運動ニューロンに関する研究はほとんど進んでいない。本研究は、これまで我々が行ってきたALS原因遺伝子ALS2と患者iPS細胞研究の成果を発展させ、世界初となるヒト上位運動ニューロン変性モデルを作製し、ALS2分子ネットワーク異常を中軸にした細胞機能解析を行うことにより、ALS患者における上位運動ニューロン変性分子メカニズムの解明に挑むものである。

Outline of Final Research Achievements

ALS is a fatal neurodegenerative disorder characterized by a selective loss of motor neurons. The aim of this study was to delineate molecular function of ALS2, a causative gene product of upper motor neuron-dominated motor neuron diseases, thereby clarifying molecular mechanism for upper motor neuron dysfunction and death. We conducted four sets of studies; 1) differentiation methods for upper motor neurons from patient-derived iPSCs, 2) organelle transport in the axon, 3) the structure-function relationship for the ALS2 complex, and 4) the intracellular ALS2 molecular network and their physiological functions. We demonstrated that 1) ALS2 plays a role in the regulation of Rab17-associated endosomal trafficking, 2) the intrinsically disordered region of ALS2 is implicated not only in the regulation of intracellular localization but also in the self-oligomerization of ALS2, and 3) the high-molecular weight ALS2 complex represents a unique form of ALS2-homophilic oligomers in the CNS.

Academic Significance and Societal Importance of the Research Achievements

ALSは、上位運動ニューロンと下位運動ニューロンの両者がともに侵される疾患であるにもかかわらず、これまでの疾患発症メカニズムに関する研究は、ほとんど例外なく脊髄の下位運動ニューロンを対象としていた。ALSの治療法や治療薬を開発するためには、発症メカニズムを正確に理解することが前提条件であり、よって下位運動ニューロンのみならず上位運動ニューロンの変性メカニズムを解明することは極めて重要である。本研究により、ALS2の分子機能の一端が明らかにされたことは、これまでほとんど研究の進んでいない上位運動ニューロン変性メカニズムの解明にとって重要な一歩である。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (61 results)

All 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (3 results) Journal Article (15 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 14 results,  Open Access: 10 results) Presentation (38 results) (of which Int'l Joint Research: 8 results,  Invited: 3 results) Book (1 results) Remarks (4 results)

  • [Int'l Joint Research] University of Torino(イタリア)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] West China Hospital, Sichuan University(中国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] Sichuan University(中国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Central nervous system specific high molecular weight ALS2/alsin homophilic complex is enriched in mouse brain synaptosomes2023

    • Author(s)
      Sato Kai、Suzuki-Utsunomiya Kyoko、Mitsui Shun、Ono Suzuka、Shimakura Kento、Otomo Asako、Hadano Shinji
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 638 Pages: 168-175

    • DOI

      10.1016/j.bbrc.2022.11.061

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic and clinical characteristics of ALS patients with NEK1 gene variants2023

    • Author(s)
      Jiang Qirui、Lin Junyu、Wei Qianqian、Li Chunyu、Hou Yanbing、Zhang Lingyu、Ou Ruwei、Liu Kuncheng、Yang Tianmi、Xiao Yi、Hadano Shinji、Shang Huifang
    • Journal Title

      Neurobiology of Aging

      Volume: 123 Pages: 191-199

    • DOI

      10.1016/j.neurobiolaging.2022.11.001

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] SQSTM1, a protective factor of SOD1-linked motor neuron disease, regulates the accumulation and distribution of ubiquitinated protein aggregates in neuron2022

    • Author(s)
      Mitsui Shun、Otomo Asako、Sato Kai、Ishiyama Masahito、Shimakura Kento、Okada-Yamaguchi Chisa、Warabi Eiji、Yanagawa Toru、Aoki Masashi、Shang Hui-Fang、Hadano Shinji
    • Journal Title

      Neurochemistry International

      Volume: 158 Pages: 105364-105364

    • DOI

      10.1016/j.neuint.2022.105364

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Personalized Treatment for Infantile Ascending Hereditary Spastic Paralysis Based on In Silico Strategies2022

    • Author(s)
      Rossi Sebastiano Matteo、Ermondi Giuseppe、Sato Kai、Otomo Asako、Hadano Shinji、Caron Giulia
    • Journal Title

      Molecules

      Volume: 27 Issue: 20 Pages: 7063-7063

    • DOI

      10.3390/molecules27207063

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] De-erosion of X chromosome dosage compensation by the editing of XIST regulatory regions restores the differentiation potential in hPSCs2022

    • Author(s)
      Motosugi Nami、Sugiyama Akiko、Okada Chisa、Otomo Asako、Umezawa Akihiro、Akutsu Hidenori、Hadano Shinji、Fukuda Atsushi
    • Journal Title

      Cell Reports Methods

      Volume: 2 Issue: 12 Pages: 100352-100352

    • DOI

      10.1016/j.crmeth.2022.100352

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] AI-based protein structure databases have the potential to accelerate rare diseases research: AlphaFoldDB and the case of IAHSP/Alsin2022

    • Author(s)
      Rossi Sebastiano Matteo、Ermondi Giuseppe、Hadano Shinji、Caron Giulia
    • Journal Title

      Drug Discovery Today

      Volume: 27 Issue: 6 Pages: 1652-1660

    • DOI

      10.1016/j.drudis.2021.12.018

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] High-throughput quantitative analysis of axonal transport in cultured neurons from SOD1H46R ALS mice by using a microfluidic device2022

    • Author(s)
      Otomo Asako、Ono Suzuka、Sato Kai、Mitsui Shun、Shimakura Kento、Kimura Hiroshi、Hadano Shinji
    • Journal Title

      Neuroscience Research

      Volume: 174 Pages: 46-52

    • DOI

      10.1016/j.neures.2021.07.005

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The N-terminal intrinsically disordered region mediates intracellular localization and self-oligomerization of ALS22021

    • Author(s)
      Shimakura Kento、Sato Kai、Mitsui Shun、Ono Suzuka、Otomo Asako、Hadano Shinji
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 569 Pages: 106-111

    • DOI

      10.1016/j.bbrc.2021.06.095

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] SQSTM1L341V variant that is linked to sporadic ALS exhibits impaired association with MAP1LC3 in cultured cells2021

    • Author(s)
      Nozaki Masahisa、Otomo Asako、Mitsui Shun、Ono Suzuka、Shirakawa Ryohei、Chen YongPing、Hama Yutaro、Sato Kai、Chen XuePing、Suzuki Toshiyasu、Shang Hui-Fang、Hadano Shinji
    • Journal Title

      eNeurologicalSci

      Volume: 22 Pages: 100301-100301

    • DOI

      10.1016/j.ensci.2020.100301

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Efficient differentiation and polarization of primary cultured neurons on poly(lactic acid) scaffolds with microgrooved structures.2020

    • Author(s)
      Otomo Asako、Takahashi Ueda Mahoko、Fujie Toshinori、Hasebe Arihiro、Suematsu Yoshitaka、Okamura Yosuke、Takeoka Shinji、Hadano Shinji、Nakagawa So
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 6716-6716

    • DOI

      10.1038/s41598-020-63537-z

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Monitoring the autophagy-endolysosomal system using monomeric Keima-fused MAP1LC3B2020

    • Author(s)
      Hayashi Hideki、Wang Ting、Tanaka Masayuki、Ogiwara Sanae、Okada Chisa、Ito Masatoshi、Fukunishi Nahoko、Iida Yumi、Nakamura Ayaka、Sasaki Ayumi、Amano Shunji、Yoshida Kazuhiro、Otomo Asako、Ohtsuka Masato、Hadano Shinji
    • Journal Title

      PLOS ONE

      Volume: 15 Issue: 6 Pages: e0234180-e0234180

    • DOI

      10.1371/journal.pone.0234180

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Alopecia areata susceptibility variant in MHC region impacts expressions of genes contributing to hair keratinization and is involved in hair loss2020

    • Author(s)
      Oka A、Takagi A、Komiyama E、Yoshihara N、Mano S、Hosomichi K、Suzuki S、Haida Y、Motosugi N、Hatanaka T、Kimura M、Ueda M、Nakagawa S、Miura H、Ohtsuka M、Tanaka M、Komiyama T、Otomo A、Hadano S、Mabuchi T、Beck S、Inoko H、Ikeda S.
    • Journal Title

      EBioMedicine

      Volume: 57 Pages: 102810-102810

    • DOI

      10.1016/j.ebiom.2020.102810

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ALS2, the small GTPase Rab17-interacting protein, regulates maturation and sorting of Rab17-associated endosomes2020

    • Author(s)
      Ono Suzuka、Otomo Asako、Murakoshi Shuji、Mitsui Shun、Sato Kai、Fukuda Mitsunori、Hadano Shinji
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 523 Issue: 4 Pages: 908-915

    • DOI

      10.1016/j.bbrc.2019.12.122

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] オートファジーと神経変性疾患 ― 筋萎縮性側索硬化症(ALS)と前頭側頭型認知症(FTD)を中心として2020

    • Author(s)
      秦野伸二
    • Journal Title

      医学のあゆみ

      Volume: 272 Pages: 885-891

    • Related Report
      2019 Annual Research Report
  • [Journal Article] An open-type microdevice to improve the quality of fluorescence labeling for axonal transport analysis in neurons2019

    • Author(s)
      Yokoyama S.、Otomo A.、Hadano S.、Kimura H.
    • Journal Title

      Biomicrofluidics

      Volume: 13 Issue: 3 Pages: 034104-034104

    • DOI

      10.1063/1.5090968

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Dysfunction of the autophagy-endolysosomal system in ALS and FTD2022

    • Author(s)
      Hadano, S.
    • Organizer
      日本神経学会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] マウス由来骨格筋とヒト由来下位運動ニューロンの共培養による神経筋結合部の構築とその機能評価2022

    • Author(s)
      内藤佳津子、大友麻子、秦野伸二
    • Organizer
      日本実験動物技術者協会
    • Related Report
      2022 Annual Research Report
  • [Presentation] FTLD-ALS疾患スペクトラムにおけるオートファジー・エンドリソソーム分解系の異常2022

    • Author(s)
      秦野伸二
    • Organizer
      日本認知症学会/日本老年精神医学会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 光変換蛍光タンパク質Dendra2を用いたSQSTM1細胞内動態観察2022

    • Author(s)
      島倉健人、岡田-山口千沙、湯田平陽香、岡晃、大友麻子、秦野伸二
    • Organizer
      本分子生物学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ヒトiPSCs由来下位運動ニューロンの培養と軸索表現型評価に適したマイクロデバイスの開発2022

    • Author(s)
      大友麻子、西島恵子、村上裕太、石川充、木村啓志、秦野伸二
    • Organizer
      日本分子生物学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 筋萎縮性側索硬化症責任遺伝子NEK1およびALS2の機能的連関が神経細胞表現型に及ぼす影響2022

    • Author(s)
      湯田平陽香、佐藤海、島倉健人、石川充、大友麻子、秦野伸二
    • Organizer
      日本分子生物学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 神経変性疾患のLLPS:光変換蛍光タンパク質Dendra2を用いたSQSTM1/p62-bodyの細胞内動態解析2022

    • Author(s)
      秦野伸二
    • Organizer
      東海大学総合医学研究所第18回研修会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 下位運動ニューロンと骨格筋の共培養による神経筋結合部の構築とその機能評価2022

    • Author(s)
      内藤佳津子、大友麻子、村松優作、西島恵子、木村啓志、秦野伸二
    • Organizer
      東海大学マイクロ・ナノ啓発会【Tμne】第14回学術講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 効率的下位運動ニューロン分化誘導法を用いたALS細胞モデルの作出2022

    • Author(s)
      西島恵子、大友麻子、村松優作、木村啓志、石川充、秦野伸二
    • Organizer
      東海大学マイクロ・ナノ啓発会【Tμne】第14回学術講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Structural architecture and cellular functions of juvenile motor neuron disease-causative gene product ALS2: implication of endosome maturation and endocytic recycling.2021

    • Author(s)
      Otomo, A., Ono, S., Sato, K., Mitsui, S. Shimakura, K., Fukuda, M., and Hadano, S.
    • Organizer
      Keystone Symposia/eSymposia, Neurodegenerative Diseases: Genes, Mechanisms and Therapeutics
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The intrinsically disordered region at the N-terminus of ALS2 acts as a determinant for the intracellular localization and oligomerization of ALS2.2021

    • Author(s)
      Shimakura, K., Sato, K., Mitsui, S., Ono, S., Otomo, A., and Hadano, S.
    • Organizer
      PACTALS 2021 NAGOYA (Pan-Asian Consortium for Treatment and Research in ALS)
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Neural cell-specific SQSTM1 deficiency exacerbates disease symptoms in a SOD1H46R-expressing ALS mouse model by regulating the formation and distribution of ubiquitin-positive aggregates in motor neurons.2021

    • Author(s)
      Mitsui, S., Otomo, A., Sato, K., Shimakura, K., Ishii, T., Yanagawa, T., Aoki, M., and Hadano, S.
    • Organizer
      第44回日本神経科学大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] The intrinsically disordered region at the N-terminus of ALS2 acts as a determinant for the intracellular localization and oligomerization of ALS22021

    • Author(s)
      Shimakura, K., Sato, K., Mitsui, S., Ono, S., Otomo, A., and Hadano, S.
    • Organizer
      第44回日本神経科学大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ALS2 forms a unique homophilic complex in the central nervous system, whose molecular weight is higher than those in the peripheral tissues.2021

    • Author(s)
      Sato, K., Suzuki-Utsunomiya, K., Mitsui, S., Yudahira, H., Ono, S., Shimakura, K., Otomo, A., and Hadano, S.
    • Organizer
      第44回日本神経科学大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 原始的な真核生物における形態変化に関与する分子の網羅的解析2021

    • Author(s)
      岡田-山口千沙、秦野伸二、荒木琢磨、佐々木亜由美、竹腰進、小濱一弘、金子堯子、中村彰男
    • Organizer
      第94回日本生化学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ALS2の天然変性領域はALS2細胞内局在および多量体形成に影響する2021

    • Author(s)
      島倉健人、佐藤海、三井駿、小野鈴花、大友麻子、秦野伸二
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 筋萎縮性側索硬化症(ALS)細胞モデルの構築を目指した骨格筋及下位運動ニューロン培養法の確立2021

    • Author(s)
      内藤佳津子、加納葵、藤田紘暉、大友麻子、木村啓志、秦野伸二
    • Organizer
      東海大学マイクロ・ナノ啓発会【Tμne】第13回学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] マイクロ流体デバイスを用いた神経筋接合部モデルの確立2021

    • Author(s)
      加納葵、藤田紘暉、大友麻子、秦野伸二、木村啓志
    • Organizer
      東海大学マイクロ・ナノ啓発会【Tμne】第13回学術講演会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Altered oligomeric states in pathogenic ALS2 variants associated with juvenile motor neuron diseases cause loss of ALS2-mediated endosomal function.2020

    • Author(s)
      Sato, K., Otomo, A., Ueda, M. T., Hiratsuka, Y., Suzuki-Utsunomiya, K., Sugiyama, J., Murakoshi, S., Mitsui, S., Ono, S., Nakagawa, S., Shang, H. -F., and Hadano, S.
    • Organizer
      Experimental Biology 2020/2020 ASBMB Annual Meeting
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] SQSTM1の欠損は神経細胞と末梢組織細胞のユビキチン化タンパク質動態に異なった影響をもたらす2020

    • Author(s)
      三井駿、石山政仁、島倉健人、佐藤海、小野鈴花、大友麻子、石井哲郎、柳川徹、青木正志、秦野伸二
    • Organizer
      第43回日本神経科学大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 神経終末画分におけるALS2は細胞質画分とは異なる特異的高次構造を形成する2020

    • Author(s)
      佐藤海、鈴木-宇都宮恭子、三井駿、小野鈴花、島倉健人、大友麻子、秦野伸二
    • Organizer
      第43回日本神経科学大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 細胞内膜小胞トラフィッキング異常に焦点を当てた神経変性疾患新規薬剤スクリーニング系の開発2020

    • Author(s)
      秦野伸二
    • Organizer
      東海大学卓越研究員制度シンポジウム
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Neural cell-specific SQSTM1 deficiency accelerates the onset of disease in an ALS mouse model.2020

    • Author(s)
      Mitsui, S., Ishiyama, M., Shimakura, K., Sato, K., Ono, S., Otomo, A., Ishii, T., Yanagawa, T., Aoki, M., and Hadano, S.
    • Organizer
      Keystone Symposia on Molecular and Cellular Biology, Brain Therapeutics: Disruptive Technologies and Opportunities
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ALS2 along with a novel ALS2 interacting protein Rab30 regulates morphological integrity and functions of the Golgi apparatus.2019

    • Author(s)
      Otomo, A., Onodera, W., Matsui, K., Sato, K., Mitsui, S., Ono, S., Fukuda, M., and Hadano, S.
    • Organizer
      30th International Symposium on ALS/MND
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A microdevice-based method for quantifying endolysosomal and mitochondrial axonal transport in neurons derived from a mouse model of ALS.2019

    • Author(s)
      Otomo, A., Kushida, T., Ishida, T., Araki, R., Sato, K., Mitsui, S., Ono, S., Kimura, H., and Hadano, S.
    • Organizer
      30th International Symposium on ALS/MND
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Phosphorylation state of ALS2/alsin alters its intracellular localization and endosome dynamics.2019

    • Author(s)
      Shimakura, K., Sato, K., Mitsui, S., Ono, S., Otomo, A., and Hadano, S.
    • Organizer
      30th International Symposium on ALS/MND
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ALS2 controls the intracellular localization of small GTPase Rab17 and regulates endosome maturation.2019

    • Author(s)
      Ono, S., Murakoshi, S., Sato, K., Mitsui, S., Otomo, A., Fukuda, M., and Hadano, S.
    • Organizer
      ASCB/EMBO 2019 meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 神経系特異的SQSTM1の欠損はALSマウスモデルの発症を早期化させる2019

    • Author(s)
      三井駿、石山政仁、島倉健人、佐藤海、小野鈴花、大友麻子、石井哲郎、柳川徹、青木正志、秦野伸二
    • Organizer
      第42回日本神経科学大会/第62回日本神経化学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ALS2疾患原因変異体の高次構造の変化はALS2が担うエンドソーム機能を喪失させる2019

    • Author(s)
      佐藤海、大友麻子、上田-高橋真保子、平塚結衣、鈴木-宇都宮恭子、杉山純也、村越秀治、三井駿、小野鈴花、中川草、Hui-Fang Shang、秦野伸二
    • Organizer
      第42回日本神経科学大会/第62回日本神経化学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ALSマウスモデル由来初代神経培養細胞のマイクロデバイスを用いた定量的軸索輸送解析2019

    • Author(s)
      大友麻子、串田隆志、石田智之、荒木良介、三井駿、小野鈴花、佐藤海、木村啓志、秦野伸二
    • Organizer
      第42回日本神経科学大会/第62回日本神経化学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] マイクロデバイスによるALSマウスモデル由来初代神経培養細胞の定量的軸索輸送解析2019

    • Author(s)
      大友麻子、串田隆志、石田智之、荒木良介、三井駿、佐藤海、小野鈴花、木村啓志、秦野伸二
    • Organizer
      東海大学マイクロ・ナノ啓発会【Tμne】第11回学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ALS2のリン酸化修飾がエンドゾーム動態に及ぼす影響2019

    • Author(s)
      島倉健人、佐藤海、大友麻子、秦野伸二
    • Organizer
      東海大学マイクロ・ナノ啓発会【Tμne】第11回学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ALS2タンパク質及び新規結合因子Rab17の分子機能解析2019

    • Author(s)
      小野鈴花、大友麻子、福田光則、秦野伸二
    • Organizer
      東海大学マイクロ・ナノ啓発会【Tμne】第11回学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 神経終末におけるALS2 は、特異的な高次構造を形成する2019

    • Author(s)
      佐藤海、鈴木(宇都宮)恭子、島倉健人、大友麻子、秦野伸二
    • Organizer
      東海大学マイクロ・ナノ啓発会【Tμne】第11回学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 単量体Keima融合型LC3Bによるオートファジー・エンドリソソーム系モニタリング法の開発2019

    • Author(s)
      秦野伸二、林英樹、王テイ、田中政之、荻原早苗、岡田千沙、伊藤誠敏、福西菜穂子、飯田裕美、中村彩花、佐々木亜由美、天野俊二、吉田和弘、大友麻子、大塚正人
    • Organizer
      第12回オートファジー研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ALS2はRac1活性依存的に低分子量Gタンパク質Rab17の細胞内局在を調節する2019

    • Author(s)
      小野鈴花、村越秀治、佐藤海、三井駿、大友麻子、福田光則、秦野伸二
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ALS2は神経終末画分において細胞質画分とは異なる特異的高次構造を形成する2019

    • Author(s)
      佐藤海、鈴木-宇都宮恭子、島倉健人、三井駿、小野鈴花、大友麻子、秦野伸二
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 神経系特異的SQSTM1の欠損はALSマウスモデルの発症を早期化させる2019

    • Author(s)
      三井駿、石山政仁、島倉健人、佐藤海、小野鈴花、大友麻子、石井哲郎、柳川徹、青木正志、秦野伸二
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Book] Autophagy dysfunction in Alzheimer's disease and dementia/ Chapter 1 Degradation mechanisms of cells2022

    • Author(s)
      Otomo, A. and Hadano, S. (Edited by Hamano, T. and Mutoh, T.)
    • Total Pages
      19
    • Publisher
      ACADEMIC PRESS (Elsevier)
    • Related Report
      2022 Annual Research Report
  • [Remarks] 東海大学 医学部医学科 基礎医学系生体機能学

    • URL

      http://mls.med.u-tokai.ac.jp/hadano/

    • Related Report
      2022 Annual Research Report
  • [Remarks] 東海大学医学部医学部医学科基礎医学系分子生命科学 分子神経病態科学研究室

    • URL

      http://mls.med.u-tokai.ac.jp/hadano/

    • Related Report
      2021 Annual Research Report
  • [Remarks] 分子神経病態科学研究室

    • URL

      http://mls.med.u-tokai.ac.jp/hadano/

    • Related Report
      2020 Annual Research Report
  • [Remarks] 東海大学 医学部医学科 基礎医学系分子生命科学:分子神経病態科学研究室(秦野研究室)

    • URL

      http://mls.med.u-tokai.ac.jp/hadano/

    • Related Report
      2019 Annual Research Report

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Published: 2019-04-18   Modified: 2024-01-30  

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